Insulin Antibody (2D11-H5)


Immunohistochemistry-Paraffin: Insulin Antibody (2D11-H5) [NBP2-15195] - Analysisof Insulin in formalin-fixed paraffin embedded human pancreas using Insulin antibody (1:500), peroxidase-conjugate and DAB chromogen. Note more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications Flow, ICC/IF, IHC-Fr, IHC-P
Please see the vial label for concentration. If unlisted please contact technical services.

Insulin Antibody (2D11-H5) Summary

The antibody reacts with the Insulin antigen.
Insulin is a protein consisting of an alpha-chain of 21 amino acids and a beta-chain of 30 amino acids and produced in the beta-cells of the pancreas. 2D11-H5 is a specific insulin mAb as tested by ELISA and on human pancreatic tissue. Source: A BALB/c mouse was immunised with swine insulin. The mouse spleen cells were fused with SP2/0 Ag14 cells and the clones were selected on anti-insulin activity.
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
PBS with 0.05% BSA.
0.05% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
Protein A or G purified


  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry-Frozen 0.5ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
Application Notes
Human Chromosome Location: 11p15.5
Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen and Immunoprecipitation applications cited in literature.
Read Publications using NBP2-15195.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for Insulin Antibody (2D11-H5)

  • IDDM2
  • ILPR
  • INS
  • insulin
  • IRDN
  • MODY10
  • proinsulin


Insulin is a secreted peptide hormone produced by the pancreas that regulates carbohydrate and lipid metabolism. Insulin is released from the pancreatic beta cells of the islets of Langerhans in response to secretagogues. Following its release, insulin quickly acts to both inhibit hepatic glucose production and stimulate peripheral blood glucose utilization. During this process cells in the liver, muscle, and fat are triggered to take up glucose and store it as glycogen, resulting in a decrease of blood glucose levels. From a clinical standpoint, the glucose lowering effect of insulin is its most relevant property. Insulin dysregulation, including insulin resistance, is associated with a number of pathological conditions such as diabetes, insulinoma, metabolic syndrome and polycystic ovary syndrome. Insulin is used to treat some forms of diabetes. Although antibody or auto-antibody against insulin isn't normally found in the blood, antibody may be produced in certain conditions including following an allergic response to insulin treatment for diabetes: IgG, IgM, and IgE antibody have all been described. The presence of auto-antibody, identified by an insulin antibody test, may have clinical significance by contributing to or causing insulin dysregulation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Insulin Antibody (NBP2-15195)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Insulin Antibody (NBP2-15195). (Showing 1 - 1 of 1 FAQs).

  1. Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.
    • I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.

Secondary Antibodies


Isotype Controls

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Blogs on Insulin.

ChREBP, a glucose sensitive transcription factor with role in glucose-lipids homeostasis and cancer
ChREBP (carbohydrate response element-binding protein) is a glucose responsive basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor that binds MLX and then carbohydrate response element /ChoRE for the induction of genes involved in...  Read full blog post.

Ghrelin: Targeting the Hunger Hormone to Combat Obesity and Type 2 Diabetes
As the hormone ghrelin is linked to appetite and weight gain, as well as impaired glucose-induced insulin secretion, there is considerable interest in this peptide as a potential drug target. Although the overall lack of success in this field has been...  Read full blog post.

Novus Adiponectin Reagents Help to Progress Obesity & Diabetes Research
Adiponectin is most well-known for its role in glucose metabolism and fatty acid breakdown. Adiponectin is secreted solely by adipose tissue, and a person with a higher percentage of body fat will express lower levels of Adiponectin. When higher level...  Read full blog post.

Signalling Advances in Adiponectin Antibody Research
Adiponectin is an adipocytokine protein that positively regulates metabolism of lipids and glucose by suppressing glucose production from the liver, stimulating insulin sensitivity, and increasing the rate of fatty acid oxidation and glucose uptake. I...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol INS

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