IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free

Western Blot: IL-3R alpha/CD123 Antibody (8E11C5) [NBP2-37354] - Analysis using IL3RA mAb against HEK293 (1) and IL3RA (AA: 200-305)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: IL-3R alpha/CD123 Antibody (8E11C5) [NBP2-37354] - Analysis of Hela cells using IL3RA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry: IL-3R alpha/CD123 Antibody (8E11C5) [NBP2-37354] - Analysis of lung cancer tissues using IL3RA mouse mAb with DAB staining.

Flow Cytometry: IL-3R alpha/CD123 Antibody (8E11C5) [NBP2-37354] - Analysis of Hela cells using IL3RA mouse mAb (green) and negative control (red).

Western Blot: IL-3R alpha/CD123 Antibody (8E11C5) [NBP2-37354] - Analysis using IL3RA mAb against human IL3RA recombinant protein. (Expected MW is 38.3 kDa)

Immunohistochemistry-Paraffin: IL-3R alpha/CD123 Antibody (8E11C5) [NBP2-37354] - Analysis of ovarian cancer tissues using IL3RA mouse mAb with DAB staining.

ELISA: IL-3R alpha/CD123 Antibody (8E11C5) [NBP2-37354] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 8E11C5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free Summary

• Description: Novus Biologicals Mouse IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free (NBP2-37354) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human IL-3R alpha/CD123 (AA: 200-305) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL3RA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 43.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free

• CD123 antigen
• CD123
• hIL3Ra
• hIL-3Ra
• IL-3 R alpha
• IL-3 receptor subunit alpha
• IL3R alpha
• IL-3R alpha
• IL-3R subunit alpha
• IL3R
• IL3RA
• IL-3Ra
• IL-3R-alpha
• IL3RAY
• IL3RX
• IL3RY
• interleukin 3 receptor, alpha (low affinity)
• interleukin-3 receptor subunit alpha
• MGC34174

Background

IL3RA (CD123) is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either common beta chain (CDw131) or the IL3RB chain to form high affinity heterodimeric IL3 receptors. CDw131 can complex with the Alpha subunits of the mouse IL3R, IL5R and GMCSFR to form high affinity receptors, while the IL3RB subunit is specific for IL3 but binds with low affinity. IL3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. It is present in Hematopoietic progenitors, basophils, mast cells and megakaryocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL3RA