Recombinant Human IFN-alpha 1 Protein

SDS-Page: Human Interferon alpha 1b Protein [NBP2-35891]

Product Discontinued

Recombinant Human IFN-alpha 1 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 167 amino acids corresponding to IFN-alpha 1 Source: E. coli

  Uniprot ID: P01562

  Amino Acid Sequence: MCDLPETHSL DNRRTLMLLA QMSRISPSSC LMDRHDFGFP QEEFDGNQFQ KAPAISVLHE LIQQIFNLFT TKDSSAAWDE DLLDKFCTEL YQQLNDLEAC VMQEERVGET PLMNVDSILA VKKYFRRITL YLTEKKYSPC AWEVVRAEIM RSLSLSTNLQ ERLRRKE

• Preparation Method: Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
• Details of Functionality: IFN-alpha 1 protein is fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.0 x 10^8 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IFNA1
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of IFN-alpha 1 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 19.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4, containing 4 % mannitol and 1 % HSA.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Human IFN-alpha 1 Protein

• IFL;IFN;IFNA@;IFNA13;IFN-ALPHA;IFN-alphaD;Interferon alpha-1/13;leIF D
• IFNA1
• IFNalpha 1
• IFN-alpha 1

Background

Interferon alpha-1, also named interferon alpha-D, is belonging to the type I interferon family and is encoded by the IFNA1gene in humans. Interferon alpha-1 is expressed in peripheral blood leukocytes and lymphoblastoid cells. IFNalpha-1 contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity. It plays an important role in inducing non-specific resistance against a broad range of viral infections, and also affects cell proliferation and modulates immune responses. IFNalpha-1a and IFNalpha-1b are two forms of interferon alpha-1 except for the substitution of alanine for valine at position 137. Interferon-alpha represents a group of related but distinct proteins that share over 95 % amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IFNA1
• Uniprot:
  • P01562(Human)