STAT2 [p Tyr690] Antibody (1021D) [Unconjugated]

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (11100-1) for 20 minutes. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Phospho-STAT2 (Y690) Monoclonal Antibody (Catalog # MAB2890) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Phospho-STAT2 (Y690) at approximately 113 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (11100-1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-STAT2 (Y690) at approximately 111 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human Phospho-STAT2 (Y690) Monoclonal Antibody (Catalog # MAB2890). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Induction of type I IFN signaling by viral and nonviral stimuli is inhibited in G2/M-arrested cells. Suit2 cells were treated for 25 h with the vehicle, paclitaxel, colchicine, or ruxolitinib at 500 nM. Cells were then treated with the vehicle (untreated), TransIT reagent (0.5%, vol/vol), poly(I:C) at 10 μg/ml plus TransIT reagent, IFN-alpha at 5,000 U/ml, or VSV at an MOI of 30 based on titration on BHK-21 cells. VSV was aspirated 1 h later, and medium was added to infected wells. Cells remained in treatment for a total of 4 h, after which total protein was isolated. Western blot results for STAT1 and -2 proteins and their phosphorylated forms are shown in addition to VSV proteins. GAPDH was used to confirm that protein loading was the same across the gel. Protein names and protein sizes in kilodaltons are indicated on the left and right, respectively. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30487274), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 1021D
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

STAT2 [p Tyr690] Antibody (1021D) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Phosphopeptide containing the human STAT2 Y690 site
• Modification: p Tyr690
• Specificity: Detects human phospho-STAT2 (Y690) in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: STAT2
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 0.5 ug/mL
  • Western Blot 0.05 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT2 [p Tyr690] Antibody (1021D) [Unconjugated]

• interferon alpha induced transcriptional activator
• ISGF-3
• MGC59816
• P113
• signal transducer and activator of transcription 2
• signal transducer and activator of transcription 2, 113kD
• signal transducer and activator of transcription 2, 113kDa
• STAT113
• STAT2

Background

STAT2 (signal transducer and activator of transcription 2) is a 113 kDa member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT2 is associated with type I ( alpha - and beta -) interferon signaling. All STATs contain an N‑terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT2 is phosphorylated at Y690 by receptor-associated Janus kinases (JAKs) leading to STAT2 dimerization and subsequent translocation to the nucleus to activate gene transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STAT2 Antibody (MAB2890)(4)

Publications using MAB2890

• Melania Cruciani, Silvia Sandini, Marilena P. Etna, Elena Giacomini, Romina Camilli, Martina Severa et al. Differential Responses of Human Dendritic Cells to Live or Inactivated Staphylococcus aureus: Impact on Cytokine Production and T Helper Expansion Frontiers in Immunology 2019-11-12 [PMID: 31781115]
• Christian Bressy, Gaith N. Droby, Bryant D. Maldonado, Nury Steuerwald, Valery Z. Grdzelishvili Cell Cycle Arrest in G 2 /M Phase Enhances Replication of Interferon-Sensitive Cytoplasmic RNA Viruses via Inhibition of Antiviral Gene Expression Journal of Virology 2019-02-15 [PMID: 30487274]
• Marcela Cataldi, Nirav R. Shah, Sébastien A. Felt, Valery Z. Grdzelishvili Breaking resistance of pancreatic cancer cells to an attenuated vesicular stomatitis virus through a novel activity of IKK inhibitor TPCA-1 Virology 2015-11-01 [PMID: 26331681]
• Marcela Cataldi, Nirav R. Shah, Sébastien A. Felt, Valery Z. Grdzelishvili Breaking resistance of pancreatic cancer cells to an attenuated vesicular stomatitis virus through a novel activity of IKK inhibitor TPCA-1 Virology 2015-11-01 [PMID: 26331681] (Western Blot, Human)

FAQs for STAT2 Antibody (MAB2890). (Showing 1 - 1 of 1 FAQs).

1. Does your phospho-STAT2 antibody (clone number 1021D) cross-react with mouse protein?
   • I have done a blast search between human and mouse STAT2, and it shows that although the tyrosine (Y) residues are conserved in the both species, the adjacent a.a. are different.  Therefore the peptide that was used to raise this antibody should contain the diverse a.a residues. Hence, I anticipate that this Ab will not cross react with mouse sample. 

Bioinformatics

• Gene Symbol: STAT2