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Angiogenesis Pathway Bioinformatics

Angiogenesis is the process of the formation of new blood vessels from those that already exist. There are multiple factors that work as angiogenic stimulants, including FGF, TGF-beta, and VEGF, which results in the MAPK pathway to initiate the growth process. MMPs are also important in angiogenesis, as they degrade the extracellular matrix and allow for the new blood vessels to grow from this location. There are two methods of angiogenesis: sprouting angiogenesis, where new cells grow off of a blood vessel and fuse together to form a shared lumen, and intussusceptive angiogenesis, where the capillary wall moves into the lumen and causes the blood vessel to split into two. Angiogenesis is an important function in the oxygenation of tissues, and can help in various functions including wound healing and the treatment of vascular diseases such as heart disease, high blood pressure, and diabetes. An abnormal rate of angiogenesis, however, can cause many problems including the proliferation of cancerous tumors, diabetic ulcers, and cardiovascular diseases. Many scientists have studied factors of angiogenesis, and treatments have been created that involve either the inhibition or activation of blood vessel growth.

Angiogenesis Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Angiogenesis below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 2964 products for the study of the Angiogenesis Pathway that can be applied to Flow Cytometry, Chromatin Immunoprecipitation, Western Blot, Immunocytochemistry/Immunofluorescence, Chromatin Immunoprecipitation (ChIP), Immunohistochemistry from our catalog of antibodies and ELISA kits.

NB100-1642
Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - MS1 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100.  The cells were incubated with anti-CD31/PECAM-1 (MEC 7.46) at 5.0 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution.  Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution.  Nuclei were counterstained with DAPI (Blue).  Cells were imaged using a 40X objective.Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - A surface stain was performed on MS-1 Cells with CD31 (MEC 7.46) antibody NB100-1642(blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems).

Rat Monoclonal
Species Mouse
Applications Flow, ICC/IF, IHC

17 Publications
AF644
Recombinant Mouse VEGF R2/KDR/Flk‑1 Fc Chimera (Catalog # <A class=NoLineLink href=VEGF R2/KDR/Flk‑1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using 15 µg/mL Mouse VEGF R2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF644) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit  (brown; Catalog # <A class=

Goat Polyclonal
Species Mouse
Applications WB, Flow, IHC

     2 Reviews

35 Publications
NB110-41083

Goat Polyclonal
Species Human
Applications WB, ChIP, ELISA

10 Publications
NB100-56749
Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Akt1 [p Ser473] Antibody (104A282) [NB100-56749] - Total protein from mouse 3T3 cells treated with and without PDGF (50 ng/ml) for the indicated times was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-AKT1 (NBP2-01725) and 2 ug/ml pS473 AKT1 in 1% BSA in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the detection of phosphorylated AKT1 in response to PDGF treatment compared to total AKT1 protein.Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:250 dilution of pSer473 AKT1 antibody (clone 104A282) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of phosphor-AKT (Ser-473) in the cancer cells as well as the stromal cells. Some cancer cells depicted nuclear stianing also.

Mouse Monoclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

     1 Review

8 Publications
NB100-105
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review.Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the HRP conjugate of NB100-105. Detection of 50ug cobalt chloride induced COS-7 nuclear extracts (NB800-PC26) using NB100-105.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ChIP, ELISA

     32 Reviews

679 Publications
NB600-1071
Immunocytochemistry/Immunofluorescence: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 antibody was tested in WEHI-3 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse small intestine using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. This antibody specifically labelled the endothelial cells in blood vessels located primarily in the sub-mucosa, and of that of the mucosa muscularis and the mucosal lacteal.

Rat Monoclonal
Species Mouse, Rat
Applications WB, ELISA, Flow

17 Publications
AF1095
Western blot shows lysates of A431 human epithelial carcinoma cell line untreated<br>(‑) or treated (+) with 100 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Phospho-EGFR (Y1173) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human EGF (Catalog # <a class=

Rabbit Polyclonal
Species Human
Applications WB, Simple Western, IHC

5 Publications
NBP1-33050
Western Blot: MVD Antibody [NBP1-33050] - A. 30 ug 293T whole lysate/extract, B. 30 ug A431 whole cell lysate/extract, C. 30 ug HeLa whole cell lysate/extract. D. 30 ug A37C whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.Immunocytochemistry/Immunofluorescence: MVD Antibody [NBP1-33050] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

NB100-689
Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded colon carcinoma stained with COX-2 antibody.Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded human colon carcinoma stained with COX2 antibody.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, IHC

     1 Review

14 Publications
NB200-193
Western Blot: MMP-2 Antibody [NB200-193] - WB analysis of recombinant pro and active forms of Human MMP-2 protein (left lane) and crude homogenate of the injured Rat peripheral nerve (right lane) using MMP-2 antibody at 0.5 ug/ml concentration. The antibody detected both pro as well as cleaved/active forms of MMP-2.Immunocytochemistry/Immunofluorescence: MMP-2 Antibody [NB200-193] - analysis of MMP-2 in HeLa cells using anti-MMP-2 antibody. Image from verified customer review.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     4 Reviews

38 Publications