Recombinant Human IFN-beta Protein, CF


Recombinant Human IFN-beta (Catalog # 8499-IF/CF) exhibits anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 5.00-30.0 more

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IFN-beta Protein, CF Summary

Additional Information
S.A approximately 2.8x10^8 IU/mg compared to WHO standard
Details of Functionality
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 5.00-30.0 pg/mL.

Chinese Hamster Ovary cell line, CHO-derived human IFN-beta protein
Accession #
N-terminal Sequence
Met22 & Ser23
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
20-26 kDa, reducing conditions
Read Publications using
8499-IF/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS.
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile water.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-beta Protein, CF

  • Fibroblast interferon
  • IFB
  • IFF
  • IFNB
  • IFNB1
  • IFNbeta
  • IFN-beta
  • interferon beta
  • interferon, beta 1, fibroblast
  • MGC96956


Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature human IFN-beta shares 47% and 46% amino acid sequence identity with the mouse and rat proteins, respectively. Fibroblasts are the major producers of IFN-beta , but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogen exposure (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). Following secretion, IFN-beta signals through the heterodimeric IFN-alpha / beta Receptor and activates the JAK/STAT signaling pathway (5-8). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (9). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (10-14).
  1. González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
  2. Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
  3. Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
  4. Häcker, H. et al. (2006) Nature 439:204.
  5. Honda, K. et al. (2006) Nat. Rev. Immunol. 6:644.
  6. Platanias, L.C. (2005) Nat. Rev. Immunol. 5:375.
  7. Fasler-Kan, E. et al. (1998) Eur. J. Biochem. 254:514.
  8. Matikainen, S. et al. (1999) Blood 93:1980.
  9. Teige, I. et al. (2003) J. Immunol. 170:4776.
  10. Shinohara, M.L. et al. (2008) Immunity 29:68.
  11. Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
  12. Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
  13. Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
  14. Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.

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Publications for IFN-beta (8499-IF/CF)(38)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - C. aethiops.

We have publications tested in 5 applications: Bioassay, Cell Culture, Differentiation, Spheroid Culture, Stimulation.

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Cell Culture
Spheroid Culture
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Primate - C. aethiops
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Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using 8499-IF/CF Applications Species
A Ta, R Ricci-Azev, SO Vasudevan, SS Wright, P Kumari, MS Havira, M Surendran, VA Rathinam, SK Vanaja A bacterial autotransporter impairs innate immune responses by targeting the transcription factor TFE3 Nature Communications, 2023-04-11;14(1):2035. 2023-04-11 [PMID: 37041208] (Bioassay, Human) Bioassay Human
J Nassour, LG Aguiar, A Correia, TT Schmidt, L Mainz, S Przetocka, C Haggblom, N Tadepalle, A Williams, MN Shokhirev, SC Akincilar, V Tergaonkar, GS Shadel, J Karlseder Telomere-to-mitochondria signalling by ZBP1 mediates replicative crisis Nature, 2023-02-08;614(7949):767-773. 2023-02-08 [PMID: 36755096] (Bioassay, Human) Bioassay Human
WC Ng, SS Kwek, B Sun, M Yousefi, EZ Ong, HC Tan, AS Puschnik, KR Chan, YS Ooi, EE Ooi A fast-growing dengue virus mutant reveals a dual role of STING in response to infection Open Biology, 2022-12-14;12(12):220227. 2022-12-14 [PMID: 36514984] (Bioassay, Human) Bioassay Human
MW Kenaston, OH Pham, MJ Petit, PS Shah Transcriptomic profiling implicates PAF1 in both active and repressive immune regulatory networks BMC Genomics, 2022-11-30;23(1):787. 2022-11-30 [PMID: 36451099] (Stimulation, Human) Stimulation Human
LE Langbein, E Sementino, Z Zhong, W Jiang, L Li, JR Testa, H Yang Data supporting the roles of BAP1, STING, and IFN-beta in ISGF3 activation in ccRCC Data in Brief, 2022-11-11;45(0):108743. 2022-11-11 [PMID: 36426057] (Bioassay, Human) Bioassay Human
ND Furtado, MM Gómez, IS de Mello, DR Fernandes, MC Bonaldo Phenotypic and Genetic Studies of the Viral Lineage Associated with the Recent Yellow Fever Outbreak in Brazil Viruses, 2022-08-19;14(8):. 2022-08-19 [PMID: 36016440] (Bioassay, Primate - C. aethiops) Bioassay Primate - C. aethiops
E Padron-Reg, M Ulaszewska, AD Douglas, AVS Hill, AJ Spencer STING-pathway modulation to enhance the immunogenicity of adenoviral-vectored vaccines Scientific Reports, 2022-08-24;12(1):14464. 2022-08-24 [PMID: 36002507] (Bioassay, Human, Mouse) Bioassay Human, Mouse
NR Choudhury, I Trus, G Heikel, M Wolczyk, J Szymanski, A Bolembach, RM Dos Santos, N Smith, M Trubitsyna, E Gaunt, P Digard, G Michlewski TRIM25 inhibits influenza A virus infection, destabilizes viral mRNA, but is redundant for activating the RIG-I pathway Nucleic Acids Research, 2022-07-08;0(0):. 2022-07-08 [PMID: 35736141] (Bioassay, Human) Bioassay Human
KK Jang, ME Kaczmarek, S Dallari, YH Chen, T Tada, J Axelrad, NR Landau, KA Stapleford, K Cadwell Variable susceptibility of intestinal organoid-derived monolayers to SARS-CoV-2 infection PloS Biology, 2022-03-31;20(3):e3001592. 2022-03-31 [PMID: 35358182] (Differentiation, Differentiation, Human) Differentiation, Differentiation Human
A Fedoriw, L Shi, S O'Brien, KN Smitheman, Y Wang, J Hou, C Sherk, S Rajapurkar, J Laraio, LJ Williams, C Xu, G Han, Q Feng, MT Bedford, L Wang, O Barbash, RG Kruger, P Hwu, HP Mohammad, W Peng Inhibiting Type I arginine methyltransferase activity promotes the T cell mediated antitumor immune response Cancer Immunology Research, 2022-04-01;0(0):. 2022-04-01 [PMID: 35181787] (Bioassay, Human) Bioassay Human
Show All 38 Publications.

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STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also ...  Read full blog post.

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Gene Symbol IFNB1