Recombinant Human IFN-beta Protein, CF


Recombinant Human IFN-beta (Catalog # 8499-IF/CF) exhibits anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 5-30 pg/mL.

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IFN-beta Protein, CF Summary

Additional Information
S.A approximately 2.8x10^8 IU/mg compared to WHO standard
Details of Functionality
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 5-30 pg/mL.
The specific activity of Recombinant Human IFN-beta is approximately 2.8 x 108 IU/mg, which is calibrated against human IFN- beta WHO International Standard (NIBSC code: 00/572).Standard (NIBSC code: 00/572).

Chinese Hamster Ovary cell line, CHO-derived human IFN-beta protein
Accession #
N-terminal Sequence
Met22 & Ser23
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
20-26 kDa, reducing conditions
Read Publications using
8499-IF/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS.
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile water.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-beta Protein, CF

  • Fibroblast interferon
  • IFB
  • IFF
  • IFNB
  • IFNB1
  • IFNbeta
  • IFN-beta
  • interferon beta
  • interferon, beta 1, fibroblast
  • MGC96956


Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature human IFN-beta shares 47% and 46% amino acid sequence identity with the mouse and rat proteins, respectively. Fibroblasts are the major producers of IFN-beta , but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogen exposure (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). Following secretion, IFN-beta signals through the heterodimeric IFN-alpha / beta Receptor and activates the JAK/STAT signaling pathway (5-8). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (9). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (10-14).
  1. González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
  2. Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
  3. Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
  4. Häcker, H. et al. (2006) Nature 439:204.
  5. Honda, K. et al. (2006) Nat. Rev. Immunol. 6:644.
  6. Platanias, L.C. (2005) Nat. Rev. Immunol. 5:375.
  7. Fasler-Kan, E. et al. (1998) Eur. J. Biochem. 254:514.
  8. Matikainen, S. et al. (1999) Blood 93:1980.
  9. Teige, I. et al. (2003) J. Immunol. 170:4776.
  10. Shinohara, M.L. et al. (2008) Immunity 29:68.
  11. Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
  12. Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
  13. Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
  14. Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.

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Publications for IFN-beta (8499-IF/CF)(30)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: Bioassay, Cell Culture, Differentiation, Spheroid Culture, Stimulation.

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Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using 8499-IF/CF Applications Species
KK Jang, ME Kaczmarek, S Dallari, YH Chen, T Tada, J Axelrad, NR Landau, KA Stapleford, K Cadwell Variable susceptibility of intestinal organoid-derived monolayers to SARS-CoV-2 infection PloS Biology, 2022;20(3):e3001592. 2022 [PMID: 35358182] (Differentiation, Human) Differentiation Human
A Fedoriw, L Shi, S O'Brien, KN Smitheman, Y Wang, J Hou, C Sherk, S Rajapurkar, J Laraio, LJ Williams, C Xu, G Han, Q Feng, MT Bedford, L Wang, O Barbash, RG Kruger, P Hwu, HP Mohammad, W Peng Inhibiting Type I arginine methyltransferase activity promotes the T cell mediated antitumor immune response Cancer Immunology Research, 2022;0(0):. 2022 [PMID: 35181787] (Bioassay, Human) Bioassay Human
NL Ka, GY Lim, S Hwang, SS Kim, MO Lee IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer Cell Reports, 2021;37(12):110138. 2021 [PMID: 34936865] (Bioassay, Human) Bioassay Human
S Wüst, P Schad, S Burkart, M Binder Comparative Analysis of Six IRF Family Members in Alveolar Epithelial Cell-Intrinsic Antiviral Responses Cells, 2021;10(10):. 2021 [PMID: 34685580] (Stimulation, Human) Stimulation Human
C Winkler, M King, J Berthe, D Ferraioli, A Garuti, F Grillo, J Rodriguez-, L Ferrando, N Chopin, I Ray-Coquar, O Delpuech, D Bedognetti, A Ballestrer, E Leo, G Zoppoli SLFN11 captures cancer-immunity interactions associated with platinum sensitivity in high-grade serous ovarian cancer JCI Insight, 2021;6(18):. 2021 [PMID: 34549724] (Bioassay, Human) Bioassay Human
C Prelli Boz, R Nchioua, M Volcic, L Koepke, J Krüger, D Schütz, S Heller, CM Stürzel, D Kmiec, C Conzelmann, J Müller, F Zech, E Braun, R Gro beta , L Wettstein, T Weil, J Wei beta , F Diofano, AA Rodríguez, S Wiese, D Sauter, J Münch, C Goffinet, A Catanese, M Schön, TM Boeckers, S Stenger, K Sato, S Just, A Kleger, KMJ Sparrer, F Kirchhoff IFITM proteins promote SARS-CoV-2 infection and are targets for virus inhibition in vitro Nature Communications, 2021;12(1):4584. 2021 [PMID: 34321474] (Bioassay, Human) Bioassay Human
X Yang, X Zhao, Y Zhu, Y Shen, Y Wang, P Lu, Z Jiang, H Pan, J Yang, J Xun, L Zhao, J Wang, Z Liang, X Shen, Y Liang, Q Lin, H Liang, L Jin, D Zhang, J Liu, B Wang, S Jiang, J Xu, H Wu, H Lu, H Zhu FKBP3 Induces Human Immunodeficiency Virus Type 1 Latency by Recruiting Histone Deacetylase 1/2 to the Viral Long Terminal Repeat MBio, 2021;0(0):e0079521. 2021 [PMID: 34281390] (Bioassay, Human) Bioassay Human
DP Strange, B Jiyarom, H Sadri-Arde, LH Cazares, TA Kenny, MD Ward, S Verma Paracrine IFN Response Limits ZIKV Infection in Human Sertoli Cells Frontiers in Microbiology, 2021;12(0):667146. 2021 [PMID: 34079533] (Bioassay, Human) Bioassay Human
X Yu, L Zhang, J Shen, Y Zhai, Q Jiang, M Yi, X Deng, Z Ruan, R Fang, Z Chen, X Ning, Z Jiang The STING phase-separator suppresses innate immune signalling Nature Cell Biology, 2021;23(4):330-340. 2021 [PMID: 33833429] (Bioassay, Human) Bioassay Human
R Tian, AJ Samelson, VV Rezelj, M Chen, GN Ramadoss, X Guo, AM Kain, QD Tran, SA Lim, I Lui, J Nunez, SJ Rockwood, N Liu, J Carlson-St, J Oki, T Maures, K Holden, JS Weissman, JA Wells, B Conklin, M Vignuzzi, M Kampmann BRD2 inhibition blocks SARS-CoV-2 infection in vitro by reducing transcription of the host cell receptor ACE2 bioRxiv : the preprint server for biology, 2021;0(0):. 2021 [PMID: 33501440] (Bioassay, Human) Bioassay Human
Show All 30 Publications.

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Gene Symbol IFNB1