Recombinant Human IFN-beta Protein, CF

Recombinant Human IFN-beta (Catalog # 8499-IF/CF) exhibits anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 5.00-30.0 pg/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IFN-beta Protein, CF Summary

• Additional Information: S.A approximately 2.8x10^8 IU/mg compared to WHO standard
• Details of Functionality: Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED₅₀ for this effect is 5.00-30.0 pg/mL.
  

• Source: Chinese Hamster Ovary cell line, CHO-derived human IFN-beta protein
  Met22-Asn187
• Accession #: P01574
• N-terminal Sequence: Met22 & Ser23
• Protein/Peptide Type: Recombinant Proteins
• Gene: IFNB1
• Purity: >95%, by SDS-PAGE with silver staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 20-26 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS.
• Purity: >95%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-beta Protein, CF

• Fibroblast interferon
• IFB
• IFBIFNB
• IFF
• IFNB
• IFNB1
• IFNbeta
• IFN-beta
• interferon beta
• interferon, beta 1, fibroblast
• MGC96956

Background

Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature human IFN-beta shares 47% and 46% amino acid sequence identity with the mouse and rat proteins, respectively. Fibroblasts are the major producers of IFN-beta , but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogen exposure (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). Following secretion, IFN-beta signals through the heterodimeric IFN-alpha / beta Receptor and activates the JAK/STAT signaling pathway (5-8). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (9). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (10-14).

1. González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
2. Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
3. Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
4. Häcker, H. et al. (2006) Nature 439:204.
5. Honda, K. et al. (2006) Nat. Rev. Immunol. 6:644.
6. Platanias, L.C. (2005) Nat. Rev. Immunol. 5:375.
7. Fasler-Kan, E. et al. (1998) Eur. J. Biochem. 254:514.
8. Matikainen, S. et al. (1999) Blood 93:1980.
9. Teige, I. et al. (2003) J. Immunol. 170:4776.
10. Shinohara, M.L. et al. (2008) Immunity 29:68.
11. Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
12. Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
13. Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
14. Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.

Publications for IFN-beta (8499-IF/CF)(38)

Publications using 8499-IF/CF

• A Ta, R Ricci-Azev, SO Vasudevan, SS Wright, P Kumari, MS Havira, M Surendran, VA Rathinam, SK Vanaja A bacterial autotransporter impairs innate immune responses by targeting the transcription factor TFE3 Nature Communications, 2023-04-11;14(1):2035. 2023-04-11 [PMID: 37041208] (Bioassay, Human)
• J Nassour, LG Aguiar, A Correia, TT Schmidt, L Mainz, S Przetocka, C Haggblom, N Tadepalle, A Williams, MN Shokhirev, SC Akincilar, V Tergaonkar, GS Shadel, J Karlseder Telomere-to-mitochondria signalling by ZBP1 mediates replicative crisis Nature, 2023-02-08;614(7949):767-773. 2023-02-08 [PMID: 36755096] (Bioassay, Human)
• WC Ng, SS Kwek, B Sun, M Yousefi, EZ Ong, HC Tan, AS Puschnik, KR Chan, YS Ooi, EE Ooi A fast-growing dengue virus mutant reveals a dual role of STING in response to infection Open Biology, 2022-12-14;12(12):220227. 2022-12-14 [PMID: 36514984] (Bioassay, Human)
• MW Kenaston, OH Pham, MJ Petit, PS Shah Transcriptomic profiling implicates PAF1 in both active and repressive immune regulatory networks BMC Genomics, 2022-11-30;23(1):787. 2022-11-30 [PMID: 36451099] (Stimulation, Human)
• LE Langbein, E Sementino, Z Zhong, W Jiang, L Li, JR Testa, H Yang Data supporting the roles of BAP1, STING, and IFN-beta in ISGF3 activation in ccRCC Data in Brief, 2022-11-11;45(0):108743. 2022-11-11 [PMID: 36426057] (Bioassay, Human)
• ND Furtado, MM Gómez, IS de Mello, DR Fernandes, MC Bonaldo Phenotypic and Genetic Studies of the Viral Lineage Associated with the Recent Yellow Fever Outbreak in Brazil Viruses, 2022-08-19;14(8):. 2022-08-19 [PMID: 36016440] (Bioassay, Primate - C. aethiops)
• E Padron-Reg, M Ulaszewska, AD Douglas, AVS Hill, AJ Spencer STING-pathway modulation to enhance the immunogenicity of adenoviral-vectored vaccines Scientific Reports, 2022-08-24;12(1):14464. 2022-08-24 [PMID: 36002507] (Bioassay, Human, Mouse)
• NR Choudhury, I Trus, G Heikel, M Wolczyk, J Szymanski, A Bolembach, RM Dos Santos, N Smith, M Trubitsyna, E Gaunt, P Digard, G Michlewski TRIM25 inhibits influenza A virus infection, destabilizes viral mRNA, but is redundant for activating the RIG-I pathway Nucleic Acids Research, 2022-07-08;0(0):. 2022-07-08 [PMID: 35736141] (Bioassay, Human)
• KK Jang, ME Kaczmarek, S Dallari, YH Chen, T Tada, J Axelrad, NR Landau, KA Stapleford, K Cadwell Variable susceptibility of intestinal organoid-derived monolayers to SARS-CoV-2 infection PloS Biology, 2022-03-31;20(3):e3001592. 2022-03-31 [PMID: 35358182] (Differentiation, Differentiation, Human)
• A Fedoriw, L Shi, S O'Brien, KN Smitheman, Y Wang, J Hou, C Sherk, S Rajapurkar, J Laraio, LJ Williams, C Xu, G Han, Q Feng, MT Bedford, L Wang, O Barbash, RG Kruger, P Hwu, HP Mohammad, W Peng Inhibiting Type I arginine methyltransferase activity promotes the T cell mediated antitumor immune response Cancer Immunology Research, 2022-04-01;0(0):. 2022-04-01 [PMID: 35181787] (Bioassay, Human)
• NL Ka, GY Lim, S Hwang, SS Kim, MO Lee IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer Cell Reports, 2021-12-21;37(12):110138. 2021-12-21 [PMID: 34936865] (Bioassay, Human)
• S Wüst, P Schad, S Burkart, M Binder Comparative Analysis of Six IRF Family Members in Alveolar Epithelial Cell-Intrinsic Antiviral Responses Cells, 2021-09-30;10(10):. 2021-09-30 [PMID: 34685580] (Stimulation, Human)
• C Winkler, M King, J Berthe, D Ferraioli, A Garuti, F Grillo, J Rodriguez-, L Ferrando, N Chopin, I Ray-Coquar, O Delpuech, D Bedognetti, A Ballestrer, E Leo, G Zoppoli SLFN11 captures cancer-immunity interactions associated with platinum sensitivity in high-grade serous ovarian cancer JCI Insight, 2021-09-22;6(18):. 2021-09-22 [PMID: 34549724] (Bioassay, Human)
• C Prelli Boz, R Nchioua, M Volcic, L Koepke, J Krüger, D Schütz, S Heller, CM Stürzel, D Kmiec, C Conzelmann, J Müller, F Zech, E Braun, R Gro beta , L Wettstein, T Weil, J Wei beta , F Diofano, AA Rodríguez, S Wiese, D Sauter, J Münch, C Goffinet, A Catanese, M Schön, TM Boeckers, S Stenger, K Sato, S Just, A Kleger, KMJ Sparrer, F Kirchhoff IFITM proteins promote SARS-CoV-2 infection and are targets for virus inhibition in vitro Nature Communications, 2021-07-28;12(1):4584. 2021-07-28 [PMID: 34321474] (Bioassay, Human)
• X Yang, X Zhao, Y Zhu, Y Shen, Y Wang, P Lu, Z Jiang, H Pan, J Yang, J Xun, L Zhao, J Wang, Z Liang, X Shen, Y Liang, Q Lin, H Liang, L Jin, D Zhang, J Liu, B Wang, S Jiang, J Xu, H Wu, H Lu, H Zhu FKBP3 Induces Human Immunodeficiency Virus Type 1 Latency by Recruiting Histone Deacetylase 1/2 to the Viral Long Terminal Repeat MBio, 2021-07-20;0(0):e0079521. 2021-07-20 [PMID: 34281390] (Bioassay, Human)
• DP Strange, B Jiyarom, H Sadri-Arde, LH Cazares, TA Kenny, MD Ward, S Verma Paracrine IFN Response Limits ZIKV Infection in Human Sertoli Cells Frontiers in Microbiology, 2021-05-17;12(0):667146. 2021-05-17 [PMID: 34079533] (Bioassay, Human)
• X Yu, L Zhang, J Shen, Y Zhai, Q Jiang, M Yi, X Deng, Z Ruan, R Fang, Z Chen, X Ning, Z Jiang The STING phase-separator suppresses innate immune signalling Nature Cell Biology, 2021-04-08;23(4):330-340. 2021-04-08 [PMID: 33833429] (Bioassay, Human)
• R Tian, AJ Samelson, VV Rezelj, M Chen, GN Ramadoss, X Guo, AM Kain, QD Tran, SA Lim, I Lui, J Nunez, SJ Rockwood, N Liu, J Carlson-St, J Oki, T Maures, K Holden, JS Weissman, JA Wells, B Conklin, M Vignuzzi, M Kampmann BRD2 inhibition blocks SARS-CoV-2 infection in vitro by reducing transcription of the host cell receptor ACE2 bioRxiv : the preprint server for biology, 2021-09-20;0(0):. 2021-09-20 [PMID: 33501440] (Bioassay, Human)
• S Trump, S Lukassen, MS Anker, RL Chua, J Liebig, L Thürmann, VM Corman, M Binder, J Loske, C Klasa, T Krieger, BP Hennig, M Messingsch, F Pott, J Kazmierski, S Twardziok, JP Albrecht, J Eils, S Hadzibegov, A Lena, B Heidecker, T Bürgel, J Steinfeldt, C Goffinet, F Kurth, M Witzenrath, MT Völker, SD Müller, UG Liebert, N Ishaque, L Kaderali, LE Sander, C Drosten, S Laudi, R Eils, C Conrad, U Landmesser, I Lehmann Hypertension delays viral clearance and exacerbates airway hyperinflammation in patients with COVID-19 Nature Biotechnology, 2020-12-24;0(0):. 2020-12-24 [PMID: 33361824] (Bioassay, Human)
• JE Rexach, D Polioudaki, A Yin, V Swarup, TS Chang, T Nguyen, A Sarkar, L Chen, J Huang, LC Lin, W Seeley, JQ Trojanowsk, D Malhotra, DH Geschwind Tau Pathology Drives Dementia Risk-Associated Gene Networks toward Chronic Inflammatory States and Immunosuppression Cell Rep, 2020-11-17;33(7):108398. 2020-11-17 [PMID: 33207193] (Cell Culture, Human)
• J Stebbing, G Sánchez Ni, M Falcone, S Youhanna, P Richardson, S Ottaviani, JX Shen, C Sommerauer, G Tiseo, L Ghiadoni, A Virdis, F Monzani, LR Rizos, F Forfori, A Avendaño-C, S De Marco, L Carrozzi, F Lena, PM Sánchez-Ju, LG Lacerenza, N Cesira, D Caldevilla, A Perrella, L Niccoli, LS Méndez, D Matarrese, D Goletti, YJ Tan, V Monteil, G Dranitsari, F Cantini, A Farcomeni, S Dutta, SK Burley, H Zhang, M Pistello, W Li, M Mas Romero, F Andrés Pre, RS Simón-Tale, R García-Mol, C Kutter, JH Felce, ZF Nizami, AG Miklosi, JM Penninger, F Menichetti, A Mirazimi, P Abizanda, VM Lauschke JAK inhibition reduces SARS-CoV-2 liver infectivity and modulates inflammatory responses to reduce morbidity and mortality Sci Adv, 2021-01-01;0(0):. 2021-01-01 [PMID: 33187978] (Spheroid Culture, Human)
• R da Silva A, LG Quiambao, F Soldevila, A Sutherland, B Peters, A Sette Lack of evidence supporting a role of IFN-&amp;beta and TGF-&amp;beta in differential polarization of Bordetella pertussis specific-T cell responses Cytokine, 2020-09-29;137(0):155313. 2020-09-29 [PMID: 33002739] (Bioassay, Human)
• CH Kerr, MA Skinnider, DDT Andrews, AM Madero, QWT Chan, RG Stacey, N Stoynov, E Jan, LJ Foster Dynamic rewiring of the human interactome by interferon signaling Genome Biol., 2020-06-15;21(1):140. 2020-06-15 [PMID: 32539747] (Bioassay, Human)
• D Li, R Wu, W Guo, L Xie, Z Qiao, S Chen, J Zhu, C Huang, J Huang, B Chen, Y Qin, F Xu, F Ma STING-Mediated IFI16 Degradation Negatively Controls Type I Interferon Production Cell Rep, 2019-10-29;29(5):1249-1260.e4. 2019-10-29 [PMID: 31665637] (Cell Culture, Human)
• TE Ginting, S Christian, YO Larasati, J Suryatengg, IM Suriaprana, G Mathew Antiviral interferons induced by Newcastle disease virus (NDV) drive a tumor-selective apoptosis Sci Rep, 2019-10-22;9(1):15160. 2019-10-22 [PMID: 31641164] (Bioassay, Human)
• W Guo, J Wei, X Zhong, R Zang, H Lian, MM Hu, S Li, HB Shu, Q Yang SNX8 modulates the innate immune response to RNA viruses by regulating the aggregation of VISA Cell. Mol. Immunol., 2019-09-11;0(0):. 2019-09-11 [PMID: 31511639] (Bioassay, Human)
• C Good, AI Wells, CB Coyne Type III interferon signaling restricts enterovirus 71 infection of goblet cells Sci Adv, 2019-03-06;5(3):eaau4255. 2019-03-06 [PMID: 30854425] (Bioassay, Human)
• H Xu, SJ Xu, SJ Xie, Y Zhang, JH Yang, WQ Zhang, MN Zheng, H Zhou, LH Qu MicroRNA-122 supports robust innate immunity in hepatocytes by targeting the RTKs/STAT3 signaling pathway Elife, 2019-02-08;8(0):. 2019-02-08 [PMID: 30735121] (Bioassay, Human)
• P An, MT Sáenz Robl, AM Duray, PG Cantalupo, JM Pipas Human polyomavirus BKV infection of endothelial cells results in interferon pathway induction and persistence PLoS Pathog., 2019-01-08;15(1):e1007505. 2019-01-08 [PMID: 30620752] (Bioassay, Human)
• I Cañadas, R Thummalapa, JW Kim, S Kitajima, RW Jenkins, CL Christense, M Campisi, Y Kuang, Y Zhang, E Gjini, G Zhang, T Tian, DR Sen, D Miao, Y Imamura, T Thai, B Piel, H Terai, AR Aref, T Hagan, S Koyama, M Watanabe, H Baba, AE Adeni, CA Lydon, P Tamayo, Z Wei, M Herlyn, TU Barbie, R Uppaluri, LM Sholl, E Sicinska, J Sands, S Rodig, KK Wong, CP Paweletz, H Watanabe, DA Barbie Tumor innate immunity primed by specific interferon-stimulated endogenous retroviruses Nat. Med., 2018-07-23;0(0):. 2018-07-23 [PMID: 30038220] (Bioassay, Human)
• A Singanayag, N Glanville, JL Girkin, YM Ching, A Marcellini, JD Porter, M Toussaint, RP Walton, LJ Finney, J Aniscenko, J Zhu, MB Trujillo-T, MA Calderazzo, C Grainge, SL Loo, PC Veerati, PS Pathinayak, KS Nichol, AT Reid, PL James, R Solari, PAB Wark, DA Knight, MF Moffatt, WO Cookson, MR Edwards, P Mallia, NW Bartlett, SL Johnston Corticosteroid suppression of antiviral immunity increases bacterial loads and mucus production in COPD exacerbations Nat Commun, 2018-06-08;9(1):2229. 2018-06-08 [PMID: 29884817] (Bioassay, Human)
• MK Holly, JG Smith Adenovirus infection of human enteroids reveals interferon sensitivity and preferential infection of goblet cells J. Virol., 2018-04-13;0(0):. 2018-04-13 [PMID: 29467318] (Bioassay, Human)
• T Vougioukla, R Bao, Y Nakamura, V Saloura Protein methyltransferases and demethylases dictate CD8+ T-cell exclusion in squamous cell carcinoma of the head and neck Oncotarget, 2017-11-22;8(68):112797-112808. 2017-11-22 [PMID: 29348866] (Bioassay, Human)
• R Fang, C Wang, Q Jiang, M Lv, P Gao, X Yu, P Mu, R Zhang, S Bi, JM Feng, Z Jiang NEMO-IKK? Are Essential for IRF3 and NF-?B Activation in the cGAS-STING Pathway J. Immunol., 2017-09-22;0(0):. 2017-09-22 [PMID: 28939760] (Bioassay, Human)
• J Corry, N Arora, CA Good, Y Sadovsky, CB Coyne Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface Proc. Natl. Acad. Sci. U.S.A., 2017-08-07;0(0):. 2017-08-07 [PMID: 28784796] (Bioassay, Human)
• X Dai, M Tohyama, M Murakami, K Sayama Epidermal keratinocytes sense dsRNA via the NLRP3 inflammasome, mediating interleukin (IL)-1? and IL-18 release Exp. Dermatol, 2017-05-12;0(0):. 2017-05-12 [PMID: 28266737] (Bioassay, Human)
• HC Hsia, JE Hutti, AS Baldwin Cytosolic DNA Promotes Signal Transducer and Activator of Transcription 3 (STAT3) Phosphorylation by TANK-binding kinase 1 (TBK1) to Restrain STAT3 Activity J. Biol. Chem, 2017-02-10;0(0):. 2017-02-10 [PMID: 28188292] (Bioassay, Human)
• J Worthingto, G Spain, JF Timms Effects of ErbB2 Overexpression on the Proteome and ErbB Ligand-specific Phosphosignaling in Mammary Luminal Epithelial Cells Mol. Cell Proteomics, 2017-02-07;16(4):608-621. 2017-02-07 [PMID: 28174229] (Bioassay, Human)

Bioinformatics

• Gene Symbol: IFNB1
• Uniprot:
  • P01574()