Human IL-6 Quantikine ELISA Kit

Product Details

Summary
Product Discontinued
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    • Catalog Number
      D6050
    • Availability
      Product Discontinued

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Human IL-6 Quantikine ELISA Kit Summary

Background
The Quantikine Human IL-6 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-6 in cell culture supernates, serum, and plasma. It contains recombinant human IL-6 and antibodies raised against recombinant human IL-6 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural IL-6 showed linear curves that were parallel to the standard curv...es obtained using the E. coli-expressed Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-6.
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Specificity
Natural and recombinant human IL-6
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
IL6

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
D6050 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-6 Quantikine ELISA Kit

  • B-cell differentiation factor
  • B-cell stimulatory factor 2
  • BSF2
  • BSF-2
  • CDF
  • CTL differentiation factor
  • HSF
  • hybridoma growth factor
  • IFNB2
  • IFN-beta-2
  • IL6
  • IL-6
  • Interferon beta-2
  • interleukin 6 (interferon, beta 2)
  • interleukin BSF-2
  • interleukin-6
  • MGI-2A

Background

Interleukin 6 (IL-6) is a pleiotropic, a-helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length and shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. Cells known to express IL-6 include CD8+ T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelins), sympathetic neurons, cerebral cortex neurons, adrenal medulla chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells and pancreatic islet beta cells. IL-6 production is generally correlated with cell activation and is normally kept in control by glucocorticoids, catecholamines, and secondary sex steroids. Normal human circulating IL-6 is in the 1 pg/mL range, with slight elevations during the menstrual cycle, modest elevations in certain cancers, and large elevations after surgery.

IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 Ra, triggering IL-6 Ra association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 Ra are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 Ra elicit responses from gp130-expressing cells that lack cell surface IL-6 Ra. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 Ra is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 Ra but not from other cytokines that use gp130 as a co-receptor. 

IL-6, along with TNF-a and IL-1, drives the acute inflammatory response. IL-6 is almost solely responsible for fever and the acute phase response in the liver, and it is important in the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in conditions such as obesity, insulin resistance, inflammatory bowel disease, arthritis, and sepsis. IL-6 modulates bone resorption and is a major effector of inflammatory joint destruction in rheumatoid arthritis through its promotion of Th17 cell development and activity. It contributes to atherosclerotic plaque development and destabilization as well as the development of inflammation-associated carcinogenesis.

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D6050
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-6 (D6050)(759)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Porcine, Primate - Chlorocebus aethiops (African Green Monkey), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio cynocephalus (Yellow Baboon), Rabbit, Xenograft.

We have publications tested in 1 application: ELISA Capture.


Filter By Application
ELISA Capture
(5)
All Applications
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Human
(719)
Mouse
(17)
Rat
(5)
Porcine
(1)
Primate - Chlorocebus aethiops (African Green Monkey)
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(2)
Primate - Macaca mulatta (Rhesus Macaque)
(7)
Primate - Papio cynocephalus (Yellow Baboon)
(1)
Rabbit
(2)
Xenograft
(3)
All Species
Showing Publications 1 - 10 of 759. Show All 759 Publications.
Publications using D6050 Applications Species
Ma?uj?o-Balcerska, E;Pietras, T; Deiodinase Types 1 and 3 and Proinflammatory Cytokine Values May Discriminate Depressive Disorder Patients from Healthy Controls Journal of clinical medicine 2023-09-24 [PMID: 37834806] (Human) Human
Nederveen, JP;Mastrolonardo, AJ;Xhuti, D;Di Carlo, A;Manta, K;Fuda, MR;Tarnopolsky, MA; Novel Multi-Ingredient Supplement Facilitates Weight Loss and Improves Body Composition in Overweight and Obese Individuals: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial Nutrients 2023-08-23 [PMID: 37686725] (Human) Human
Sturm, L;Hirose, M;Stolz, L;Schultheiss, M;Zoldan, K;Reincke, M;Huber, JP;Kaeser, R;Boettler, T;Thimme, R;Albert, E;Busch, H;Künstner, A;Bettinger, D; Proton pump inhibitor treatment aggravates bacterial translocation in patients with advanced cirrhosis and portal hypertension mBio 2023-08-25 [PMID: 37623323] (Human) Human
Zhang, X;Yao, J;Li, X;Niu, N;Liu, Y;Hajek, RA;Peng, G;Westin, S;Sood, AK;Liu, J; Targeting polyploid giant cancer cells potentiates a therapeutic response and overcomes resistance to PARP inhibitors in ovarian cancer Science advances 2023-07-21 [PMID: 37478190] (Human) Human
Kitamura, N;Yamamoto, Y;Yamamoto, N;Murase, T; Rosmarinic acid ameliorates HCl-induced cystitis in rats PloS one 2023-07-18 [PMID: 37463180] (Human) Human
Katsura, D;Tsuji, S;Hayashi, K;Tokoro, S;Hoshiyama, T;Kita, N;Murakami, T; Amniotic fluid interleukin-6 and neutrophil gelatinase-associated lipocalin for predicting fetal inflammatory response syndrome based on histological chorioamnionitis and funisitis Taiwanese journal of obstetrics & gynecology 2023-07-01 [PMID: 37407186] (Human) Human
Chow, FC;Mundada, NS;Abohashem, S;La Joie, R;Iaccarino, L;Arechiga, VM;Swaminathan, S;Rabinovici, GD;Epel, ES;Tawakol, A;Hsue, PY; Psychological stress is associated with arterial inflammation in people living with treated HIV infection Brain, behavior, and immunity 2023-06-25 [PMID: 37369339] (Human) Human
Benson, S;Labrenz, F;Kotulla, S;Brotte, L;Rödder, P;Tebbe, B;Theysohn, N;Engler, H;Elsenbruch, S; Amplified gut feelings under inflammation and depressed mood: A randomized fMRI trial on interoceptive pain in healthy volunteers Brain, behavior, and immunity 2023-06-10 [PMID: 37302437] (Human) Human
Belkin, S;Benthien, J;Axt, PN;Mohr, T;Mortensen, K;Weckmann, M;Drömann, D;Franzen, KF; Impact of Heated Tobacco Products, E-Cigarettes, and Cigarettes on Inflammation and Endothelial Dysfunction International journal of molecular sciences 2023-05-29 [PMID: 37298381] (Human) Human
Komoto, S;Noma, K;Kato, T;Kobayashi, T;Nishiwaki, N;Narusaka, T;Sato, H;Katsura, Y;Kashima, H;Kikuchi, S;Ohara, T;Tazawa, H;Fujiwara, T; Conventional Cancer Therapies Can Accelerate Malignant Potential of Cancer Cells by Activating Cancer-Associated Fibroblasts in Esophageal Cancer Models Cancers 2023-05-30 [PMID: 37296933] (Human) Human