Human IL-6 Quantikine ELISA Kit

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

Human IL-6 Quantikine ELISA Kit Summary

Background
The Quantikine Human IL-6 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-6 in cell culture supernates, serum, and plasma. It contains recombinant human IL-6 and antibodies raised against recombinant human IL-6 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural IL-6 showed linear curves that were parallel to the standard curv...es obtained using the E. coli-expressed Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-6.
Show More
Specificity
Natural and recombinant human IL-6
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
IL6

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
D6050 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-6 Quantikine ELISA Kit

  • B-cell differentiation factor
  • B-cell stimulatory factor 2
  • BSF2
  • BSF-2
  • CDF
  • CTL differentiation factor
  • HSF
  • hybridoma growth factor
  • IFNB2
  • IFN-beta-2
  • IL6
  • IL-6
  • Interferon beta-2
  • interleukin 6 (interferon, beta 2)
  • interleukin BSF-2
  • interleukin-6
  • MGI-2A

Background

Interleukin 6 (IL-6) is a pleiotropic, a-helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length and shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. Cells known to express IL-6 include CD8+ T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelins), sympathetic neurons, cerebral cortex neurons, adrenal medulla chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells and pancreatic islet beta cells. IL-6 production is generally correlated with cell activation and is normally kept in control by glucocorticoids, catecholamines, and secondary sex steroids. Normal human circulating IL-6 is in the 1 pg/mL range, with slight elevations during the menstrual cycle, modest elevations in certain cancers, and large elevations after surgery.

IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 Ra, triggering IL-6 Ra association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 Ra are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 Ra elicit responses from gp130-expressing cells that lack cell surface IL-6 Ra. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 Ra is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 Ra but not from other cytokines that use gp130 as a co-receptor. 

IL-6, along with TNF-a and IL-1, drives the acute inflammatory response. IL-6 is almost solely responsible for fever and the acute phase response in the liver, and it is important in the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in conditions such as obesity, insulin resistance, inflammatory bowel disease, arthritis, and sepsis. IL-6 modulates bone resorption and is a major effector of inflammatory joint destruction in rheumatoid arthritis through its promotion of Th17 cell development and activity. It contributes to atherosclerotic plaque development and destabilization as well as the development of inflammation-associated carcinogenesis.

Customers Who Viewed This Item Also Viewed...

MAB208
Species: Hu
Applications: WB, Simple Western, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
NBP2-22471
Species: Hu, Mu, Rt, Pm, Pm
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, KD
AF5739
Species: Hu, Mu, Rt
Applications: WB
NBP2-27406
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56583
Species: Hu, Rt, Rb
Applications: WB, Flow, IHC, IHC-P, KO
NB100-56566
Species: Hu, Mu, Rt, Po, Bv, Ma
Applications: WB, ChIP, DB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, PAGE, B/N, CyTOF-ready, Dual ISH-IHC, ELISA(Cap), Flow-CS, Flow-IC, KD, KO
D6050
Species: Hu
Applications: ELISA
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-6 (D6050)(474)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, House, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio cynocephalus (Yellow Baboon), Rabbit, Xenograft.

We have publications tested in 1 application: ELISA Capture.


Filter By Application
ELISA Capture
(5)
All Applications
Filter By Species
Human
(443)
Mouse
(9)
Rat
(2)
House
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(2)
Primate - Macaca mulatta (Rhesus Macaque)
(5)
Primate - Papio cynocephalus (Yellow Baboon)
(1)
Rabbit
(2)
Xenograft
(2)
All Species
Showing Publications 1 - 10 of 474. Show All 474 Publications.
Publications using D6050 Applications Species
H Sun, Y Jiang, Y Song, X Zhang, J Wang, J Zhang, J Kang The MUC5B Mucin Is Involved in Paraquat-Induced Lung Inflammation Oxid Med Cell Longev, 2020;2020(0):7028947. 2020 [PMID: 32724493] (Mouse) Mouse
T Vlaykova, M Kurzawski, T Tacheva, D Dimov, A Anastasov, D Vlaykova, A Miteva, N O'donoghue, M Drozdzik Effects of the IL6 -174G>C promoter polymorphism and IL-6 serum levels on the progression of cutaneous malignant melanoma Oncol Lett, 2020;20(2):1781-1791. 2020 [PMID: 32724421] (Human) Human
T Pan, F Zhang, F Li, X Gao, Z Li, X Li, X Ren Shikonin blocks human lung adenocarcinoma cell migration and invasion in the inflammatory microenvironment via the IL?6/STAT3 signaling pathway Oncol. Rep., 2020;44(3):1049-1063. 2020 [PMID: 32705271] (Human) Human
HF Zhang, HB Zhang, XP Wu, YL Guo, WD Cheng, F Qian Fisetin alleviates sepsis-induced multiple organ dysfunction in mice via inhibiting p38 MAPK/MK2 signaling Acta Pharmacol. Sin., 2020;0(0):. 2020 [PMID: 32661350] (Mouse) Mouse
B Smok, K Domagalski, M Paw?owska Diagnostic and Prognostic Value of IL-6 and sTREM-1 in SIRS and Sepsis in Children Mediators Inflamm., 2020;2020(0):8201585. 2020 [PMID: 32655314] (Human) Human
JH Seo, CS Hwang, JY Yoo MTFMT deficiency correlates with reduced mitochondrial integrity and enhanced host susceptibility to intracellular infection Sci Rep, 2020;10(1):11183. 2020 [PMID: 32636430] (Human) Human
A Fujiwara, S Funaki, E Fukui, K Kimura, T Kanou, N Ose, M Minami, Y Shintani Effects of pirfenidone targeting the tumor microenvironment and tumor-stroma interaction as a novel treatment for non-small cell lung cancer Sci Rep, 2020;10(1):10900. 2020 [PMID: 32616870] (Human) Human
Z Xu, T Ke, Y Zhang, C Fu, W He Agonism of GPR120 prevented IL-1&beta-induced reduction of extracellular matrix through SOX-9 Aging (Albany NY), 2020;12(12):12074-12085. 2020 [PMID: 32580167] (Human) Human
Ž Strezoska, SM Dickerson, E Maksimova, E Chou, MM Gross, K Hemphill, T Hardcastle, M Perkett, J Stombaugh, GW Miller, EM Anderson, A Vermeulen, AVB Smith CRISPR-mediated transcriptional activation with synthetic guide RNA J. Biotechnol., 2020;319(0):25-35. 2020 [PMID: 32470463] (Human) Human
S Shin, SH Kim, KK Park, SJ Kim, JC Bae, YS Choi Effects of Anesthesia Techniques on Outcomes after Hip Fracture Surgery in Elderly Patients: A Prospective, Randomized, Controlled Trial J Clin Med, 2020;9(6):. 2020 [PMID: 32466360] (Human) Human
Show All 474 Publications.

Reviews for IL-6 (D6050) (0)

There are no reviews for IL-6 (D6050). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-6 (D6050). (Showing 1 - 2 of 2 FAQs).

  1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
    • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.
  2. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP D6050
HRP D6050
HRP D6050

Additional IL-6 Products

Bioinformatics Tool for IL-6 (D6050)

Discover related pathways, diseases and genes to IL-6 (D6050). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-6 (D6050)

Discover more about diseases related to IL-6 (D6050).
 

Pathways for IL-6 (D6050)

View related products by pathway.

PTMs for IL-6 (D6050)

Learn more about PTMs related to IL-6 (D6050).

Blogs on IL-6. Showing 1-10 of 11 blog posts - Show all blog posts.

COVID-19-Related Neurological Problems: Uncovering the Mechanisms
By Jamshed Arslan, Pharm D, PhDThere is good news and bad news related to COVID-19, a disease caused by SARS-COV-2 infection. Good news first: the vast majority of otherwise healthy individuals remain asymptomatic...  Read full blog post.

Rest in Peace: Is the Receptor Interacting Protein (RIP) Kinase-3 (RIPK3) a Protector or Offender?
By Jamshed Arslan, Pharm. D., PhD. Receptor interacting protein kinases (RIPKs) in necroptosisDeath is perhaps inevitable. Cell death can be programed and immunologically silent (apoptosis), unprogrammed and inflamm...  Read full blog post.

Gut-brain axis: microbiota influence behavior and mental well-being
By Jennifer Sokolowski, MD, PhD.Gut-microbiome interactionsOn and in our bodies, microbes outnumber our cells by about ten-to-one. Studies have revealed that the microbiome influences neurogenesis, cognition, and...  Read full blog post.

Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.

Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein...  Read full blog post.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

VEGF: Vascular endothelial growth factor
VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g...  Read full blog post.

TLR2 - I can recognize many foreign pathogens!
TLR2 is a member of the broad family of Toll-like receptors (TLR) that play an important role in pathogen recognition and innate immunity. TLRs, like other pattern recognition receptors (PRRs), recognize endogenous ligands released during cell or tiss...  Read full blog post.

MyD88 Antibodies for IL Signaling and Immunity Research
The myeloid differentiation protein MyD88 (myeloid differentiation primary response protein) was originally identified and characterized as a primary upregulated response gene in interleukin-6 mediated myeloid differentiation. Now, MyD88 is known to b...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Review this Product

Be the first to review our Human IL-6 Quantikine ELISA Kit and receive a gift card or discount.

Bioinformatics

Gene Symbol IL6