Human E-Cadherin Quantikine ELISA Kit

Product Discontinued

Human E-Cadherin Quantikine ELISA Kit Summary

• Background: The Quantikine Human E-Cadherin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human E-Cadherin in cell culture supernates, serum, plasma, urine, and saliva. It contains NS0-expressed recombinant human E-Cadherin/Fc Chimera and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human E-Cadherin showed linear curves that were parall...el to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring E-Cadherin.
  Show More
• Additional Information: Product is in the process of discontinuation and replaced by catalog # DCADE0B.
• Specificity: Natural and recombinant human E-Cadherin
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CDH1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human E-Cadherin Quantikine ELISA Kit

• Arc-1
• CAD1
• cadherin 1, E-cadherin (epithelial)
• cadherin 1, type 1, E-cadherin (epithelial)
• Cadherin-1
• calcium-dependent adhesion protein, epithelial
• CAM 120/80
• CD324 antigen
• CD324
• CDH1
• CDHE
• cell-CAM 120/80
• Cell-CAM120/80
• ECAD
• ECadherin
• E-Cadherin
• Epithelial cadherin
• LCAM
• L-CAM
• UVOE-Cadherin
• Uvomorulin

Background

The cadherin superfamily comprises a large number of cell surface glycoproteins with one or more cadherin repeats, which are involved in Ca2⁺-dependent cell-cell adhesion. Classical cadherins contain five extracellular cadherin repeats and a conserved cytoplasmic region that interacts with the actin cytoskeleton via catenins. In addition to cell adhesion, cadherins also mediate morphogenesis, cytoskeletal organization, and cell migration. In addition to the classical cadherins, the cadherin superfamily also includes desmosomal cadherins, protocadherins, 7TM cadherins, FAT family cadherins, and T-cadherin.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for E-Cadherin (DCADE0)(9)

Publications using DCADE0

• N Ali, CB Nguyen, P Chandrakes, RF Wolf, D Qu, R May, T Goretsky, J Fazili, TA Barrett, M Li, MM Huycke, MS Bronze, CW Houchen Doublecortin-like kinase 1 promotes hepatocyte clonogenicity and oncogenic programming via non-canonical &beta-catenin-dependent mechanism Sci Rep, 2020-06-29;10(1):10578. 2020-06-29 [PMID: 32601309] (Human)
• MJ Kim, BH Chi, JJ Yoo, YM Ju, YM Whang, IH Chang Structure establishment of three-dimensional (3D) cell culture printing model for bladder cancer PLoS ONE, 2019-10-22;14(10):e0223689. 2019-10-22 [PMID: 31639124] (Human)
• M Singh, XJ Tian, VS Donnenberg, AM Watson, J Zhang, LP Stabile, SC Watkins, J Xing, S Sant Targeting the temporal dynamics of hypoxia-induced tumor-secreted factors halts tumor migration Cancer Res., 2019-04-05;0(0):. 2019-04-05 [PMID: 30952634] (Human, Xenograft)
• Dong , Xiaofeng, Li , Rui, Xiu , Peng, Dong , Xuesong, Xu , Zongzhen, Zhai , Bo, Liu , Feng, Jiang , Hongchi, Sun , Xueying, Li , Jie, Qiao , Haiquan Meloxicam executes its antitumor effects against hepatocellular carcinoma in COX-2- dependent and -independent pathways. PLoS ONE, 2014-03-27;9(3):e92864. 2014-03-27 [PMID: 24675684] (Human)
• Gaida M, Steffen T, Gunther F, Tschaharganeh D, Felix K, Bergmann F, Schirmacher P, Hansch G Polymorphonuclear neutrophils promote dyshesion of tumor cells and elastase-mediated degradation of E-cadherin in pancreatic tumors. Eur J Immunol, 2012-11-02;42(12):3369-80. 2012-11-02 [PMID: 23001948] (Human)
• Zekri A, Bahnassy A, Alam El-Din H, Morsy H, Shaarawy S, Moharram N, Daoud S Serum levels of beta-catenin as a potential marker for genotype 4/hepatitis C-associated hepatocellular carcinoma. Oncol Rep, 2011-06-21;26(4):825-31. 2011-06-21 [PMID: 21701780] (Human)
• Streeck H, Kwon DS, Pyo A, Flanders M, Chevalier MF, Law K, Julg B, Trocha K, Jolin JS, Anahtar MN, Lian J, Toth I, Brumme Z, Chang JJ, Caron T, Rodig SJ, Milner DA, Piechoka-Trocha A, Kaufmann DE, Walker BD, Altfeld M Epithelial adhesion molecules can inhibit HIV-1-specific CD8+ T-cell functions. Blood, 2011-03-14;117(19):5112-22. 2011-03-14 [PMID: 21403126] (Human)
• Hung YJ, Lee CH, Chu NF Plasma protein growth arrest-specific 6 levels are associated with altered glucose tolerance, inflammation, and endothelial dysfunction. Diabetes Care, 2010-05-26;33(8):1840-4. 2010-05-26 [PMID: 20504897] (Human)
• Jonsson MV, Salomonsson S, Oijordsbakken G, Skarstein K Elevated serum levels of soluble E-cadherin in patients with primary Sjogren's syndrome. Scand. J. Immunol., 2005-12-01;62(6):552-9. 2005-12-01 [PMID: 16316423] (Human)

FAQs for E-Cadherin (DCADE0). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CDH1