Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody [NBP1-77455] - HSP70 antibody was tested at 1:25 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: HSP70/HSPA1A Antibody [NBP1-77455] - Analysis of Hsp70 in human xenograft kidney cancer using DAB with hematoxylin counterstain.
Flow Cytometry: HSP70/HSPA1A Antibody [NBP1-77455] - An intracellular stain was performed on Jurkat cells with HSP70/HSPA1A Antibody NBP1-77455AF488 (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Flow Cytometry: HSP70/HSPA1A Antibody [NBP1-77455] - Single cell suspension generated from B16-F10 tumor excised from C57BL/6 Mouse stained with Hsp70 & FITC secondary (blue) or unstained (red). [Image provided by a ...read more
Flow Cytometry: HSP70/HSPA1A Antibody [NBP1-77455] - An intracellular stain was performed on A549 cells with HSP70/HSPA1A Antibody NBP1-77455AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Simple Western: HSP70/HSPA1A Antibody [NBP1-77455] - Lane view shows a specific band for Hsp70 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 26755821). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Read 1 Review rated 3 using NBP1-77455 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for HSP70/HSPA1A Antibody
dnaK-type molecular chaperone HSP70-1
Heat shock 70 kDa protein 1/2
heat shock 70 kDa protein 1A/1B
heat shock 70kD protein 1A
heat shock 70kDa protein 1A
heat shock-induced protein
Heat shock protein 70 1A/1B (also known as HSP70, HSPA1A, HSPA1B, HSP70 1/2) is a chaperone protein which can stabilize proteins against aggregation and assist in protein folding. It has been shown to play a role in aging, viral replication, lupus, and tumor progression. Accordingly, HSP70 has been used in anti-tumor vaccines, as well as HIV and Parkinson's research.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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