HSP70/HSPA1A Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody [NBP1-77455] - HSP70 antibody was tested at 1:25 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: HSP70/HSPA1A Antibody [NBP1-77455] - Analysis of Hsp70 in human xenograft kidney cancer using DAB with hematoxylin counterstain.
Flow Cytometry: HSP70/HSPA1A Antibody [NBP1-77455] - An intracellular stain was performed on Jurkat cells with HSP70/HSPA1A Antibody NBP1-77455AF488 (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Flow Cytometry: HSP70/HSPA1A Antibody [NBP1-77455] - Single cell suspension generated from B16-F10 tumor excised from C57BL/6 Mouse stained with Hsp70 & FITC secondary (blue) or unstained (red). [Image provided by a ...read more
Flow Cytometry: HSP70/HSPA1A Antibody [NBP1-77455] - An intracellular stain was performed on A549 cells with HSP70/HSPA1A Antibody NBP1-77455AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Simple Western: HSP70/HSPA1A Antibody [NBP1-77455] - Lane view shows a specific band for Hsp70 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, PmSpecies Glossary
Applications Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.18 mg/ml

HSP70/HSPA1A Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human Hsp70 protein (between residues 550-600) [Uniprot: P08107]
Localization
Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Predicted Species
Rat (92%), Porcine (100%), Bovine (100%), Canine (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPA1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Simple Western 1:50
  • Flow Cytometry 1 : 200
  • Immunocytochemistry/Immunofluorescence 1:25
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:400
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 26755821). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 1 Review rated 3
using
NBP1-77455 in the following applications:

Publications
Read Publications using
NBP1-77455 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.18 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HSP70/HSPA1A Antibody

  • dnaK-type molecular chaperone HSP70-1
  • FLJ54303
  • FLJ54370
  • FLJ54392
  • FLJ54408
  • FLJ75127
  • Heat shock 70 kDa protein 1/2
  • heat shock 70 kDa protein 1A/1B
  • heat shock 70kD protein 1A
  • heat shock 70kDa protein 1A
  • heat shock-induced protein
  • HSP70
  • HSP70.1/HSP70.2
  • HSP70-1
  • HSP70-1/HSP70-2
  • HSP70-1A
  • HSP70I
  • HSP72
  • HSPA1
  • HSPA1A
  • HSPA1B

Background

Heat shock protein 70 1A/1B (also known as HSP70, HSPA1A, HSPA1B, HSP70 1/2) is a chaperone protein which can stabilize proteins against aggregation and assist in protein folding. It has been shown to play a role in aging, viral replication, lupus, and tumor progression. Accordingly, HSP70 has been used in anti-tumor vaccines, as well as HIV and Parkinson's research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-77455
Species: Hu, Mu, Rt, Po, Bv, Ca, Pm
Applications: Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for HSP70/HSPA1A Antibody (NBP1-77455)(3)

Review for HSP70/HSPA1A Antibody (NBP1-77455) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-77455:
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(1)
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Images Ratings Applications Species Date Details
Flow Cytometry HSP70/HSPA1A NBP1-77455
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3
reviewed by:
Subhash Gangar
Flow Mouse 08/20/2014
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Summary

ApplicationFlow Cytometry
Sample TestedMouse Melanoma B16-F10
SpeciesMouse
LotA1
CommentsRed - unstained sample, Blue - stained/treated sample and staining of Hsp70 was found to be distinct with two clear populations on the plot. Antibody did not appear to have non-specific binding.

Primary Anitbody

Dilution Ratio1:200 in 0.5% BSA/PBS; 30 minutes at 4C

Details

Detection NotesLSR II Flow Cytometer
Fixation Details4% PFA at end of staining prior to acquisition
Wash Description0.5% BSA in PBS 1 time

Comments

CommentsRed - unstained sample, Blue - stained/treated sample and staining of Hsp70 was found to be distinct with two clear populations on the plot. Antibody did not appear to have non-specific binding.

Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for HSP70/HSPA1A Antibody (NBP1-77455) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-77455AF350
Alexa Fluor 405 NBP1-77455AF405
Alexa Fluor 488 NBP1-77455AF488
Alexa Fluor 532 NBP1-77455AF532
Alexa Fluor 594 NBP1-77455AF594
Alexa Fluor 647 NBP1-77455AF647
Alexa Fluor 700 NBP1-77455AF700
Alexa Fluor 750 NBP1-77455AF750
Biotin NBP1-77455B
DyLight 350 NBP1-77455UV
DyLight 405 NBP1-77455V
DyLight 488 NBP1-77455G
DyLight 550 NBP1-77455R
DyLight 594 NBP1-77455DL594
DyLight 650 NBP1-77455C
DyLight 680 NBP1-77455FR
DyLight 755 NBP1-77455IR
FITC NBP1-77455F
Janelia Fluor 549 NBP1-77455JF549
Janelia Fluor 646 NBP1-77455JF646

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Recent Reviews

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Subhash Gangar
08/20/2014
Application: Flow
Species: Mouse

Bioinformatics

Gene Symbol HSPA1A
Uniprot