HO-1/HMOX1/HSP32 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: HO-1/HMOX1/HSP32 Antibody [NBP1-77460] - HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: HO-1/HMOX1/HSP32 Antibody [NBP1-77460] - Analysis of Heme Oxygenase 1 in mouse kidney using DAB with hematoxylin counterstain.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.17 mg/ml

HO-1/HMOX1/HSP32 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HO-1/HMOX1/HSP32 Antibody - BSA Free (NBP1-77460) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-HO-1/HMOX1/HSP32 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to a C-terminal portion of the human Heme Oxygenase 1 protein (between residues 225-275) [Uniprot: P09601]
• Localization: Microsome. Endoplasmic reticulum.
• Marker: Oxidative Stress Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HMOX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
• Application Notes: Based on customer feedback, this antibody may not be suitable for use in western blot.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.17 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HO-1/HMOX1/HSP32 Antibody - BSA Free

• bK286B10
• EC 1.14.99.3
• heat shock protein, 32-kD
• heme oxygenase (decycling) 1
• HMOX1
• HO
• HO1
• HO-1
• HO-1heme oxygenase 1
• HSP32

Background

HO-1 (also know as Heme oxygenase-1, HMOX1, HSP32), a member of the heme oxygenase family, is an enzyme for heme catabolism and cleaves heme to form biliverdin. This inducible protein reacts from hypoxia, cytokines, and oxidative stress. HO-1 is itself an antioxidant that protects cells from ROS (reactive oxygen species) by regulating ROS levels. HO-1 has been shown to inhibit the pathogenesis of immune related inflammatory diseases as well as cardiovascular disease (CVD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HO-1/HMOX1/HSP32 Antibody (NBP1-77460)(2)

Publications using NBP1-77460

• Tamosiuniene R, Manouvakhova O, Mesange P et al. Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension. Circ Res 2018-06-08 [PMID: 29545367] (Rat)
• Fouad AA, Albuali WH, Jresat Iet al. Simvastatin Treatment Ameliorates Injury of Rat Testes Induced by Cadmium Toxicity. Biol Trace Elem Res 2013-04-28 [PMID: 23625729] (IHC-P, Rat)

Bioinformatics

• Gene Symbol: HMOX1
• Entrez:
  • 3162(Human)
  • 15368(Rat)
• Uniprot:
  • P09601()