HO-1/HMOX1/HSP32 Antibodies: Novus Biologicals

NBP1-97507

HO-1/HMOX1/HSP32 Antibody (HO-1-1) - BSA Free

(1 Review)

(20 Publications)

0.05 mg

NBP1-97507

Flow Cytometry: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Analysis of 10^6 Jurkat cells stained using HO-1 monoclonal antibody (HO-1-1), (Prod. No. ADI-OSA-110), at a concentration of 50 ug/mL

Effect of Sepsis with Isoflurane or Propofol anesthesia on Liver HIF1a, Bcl2, iNOS and HO-1, Protein expression levels. (A) HIF-1 alpha protein was increased in both anesthetic groups by sepsis (Isoflurane +Sepsis, **p = 0.004 and Propofol + Sepsis, *p = 0.04). (B) Bcl-2 protein was increased in Isoflurane +Sepsis group (**p = 0.004) and no significant change in Propofol + Sepsis (*p = 0.104). (C) Inducible nitric oxide synthase [iNOS, (**p = 0.002)] and (D) Heme oxygenase-1 (HO-1, ***p = 0.0006) was increased in Isoflurane +Sepsis group. Whereas, in animals anesthetized with Propofol, three organ-protective proteins, Bcl-2, iNOS, and HO-1 expression was unchanged significantly. Data are shown in mean +/- S.D, n = 12. *denotes a statistically significant (P ⩽ 0.05) difference between animals exposed to sepsis and controls. Statistical differences between results were evaluated using the t-test. “ns

Protein expression data (upper) and representative raw data (lower) for animals anesthetized with isoflurane (bcl-2 [a median + IQR], HIF-1 alpha [b mean + 95% confidence interval (CI)], HO-1 [c mean + 95% CI for isoflurane, median + IQR for propofol], iNOS [d mean + 95% CI for isoflurane, median + IQR for propofol) *denotes a statistically significant difference between animals exposed to sepsis and controls Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31307382), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

KEAP1 modulates ferroptosis through NRF2/HO‐1 pathway in hiPSC‐derived cINs. (A) Western blot showed the expression of NRF2 and HO‐1. (B) Quantification of Fe2⁺ levels, using an iron content determination assay. (C) Measurement of MDA levels using an MDA assay kit. (D) Quantification of GSH levels using a GSH assay kit. (E) Assessment of GPX4 activity using an ELISA kit. (F) GPX4 mRNA and protein were evaluated by qRT‐PCR and Western blot analysis, respectively. (G) Measurement of lipid oxidation using C11‐BODIPY staining, a dye specific for detecting lipid reactive oxygen species. (H) Quantification of inflammatory cytokines in the cell culture supernatant using ELISA kits. *p < 0.05 and **p < 0.01 show statistical significance when compared to SCZ‐pcDNA KEAP1 group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40021790), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

ARE-primed PD-MSCs increased antioxidant and anti-inflammatory effects in ARPE19 cells. (A) Oxidative stress levels evaluated in ARPE19 cells using MitoSOX/MitoTracker staining and (B) intensity quantified by image J. The gray bar, gray hatch bar, black bar, black hatch bar means ARPE19 cells with H2O2 and ARE low, ARPE19 cells with with H2O2, ARE low and PD-MSCs, ARPE19 cells with H2O2 and ARE high and ARPE19 cells with with H2O2, ARE high and PD-MSCs. The mRNA levels of (C) SOD1 and (D) HMOX1 analyzed by qRT-PCR. The protein levels of (E) SOD1 and (F) HMOX1 analyzed by Western blot. The mRNA levels of (G) TGFB1, (H) IL6 analyzed by qRT-PCR. The protein levels of (I) TGFB1 and (J) IL6 analyzed by Western blot. Scale bar = 200 μm. Each experiment was replicated three times, resulting in a total sample size of three (n = 3). The data represent the mean +/- SEM. Statistical significance was determined by using one-way ANOVA and Tukey’s post hoc test for the comparison of groups, * p < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39056810), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-97507-0.2mg
BSA Free	NBP1-97507SS

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NBP1-31341

HO-1/HMOX1/HSP32 Antibody - BSA Free

(1 Review)

(14 Publications)

0.1 ml

NBP1-31341

<b>Biological Strategies Validation. </b>HO-1-HMOX1-HSP32-Antibody-Western-Blot-NBP1-31341-img0032.jpg

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Human renal carcinoma . Heme Oxygenase 1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Rat spleen. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HO-1/HMOX1/HSP32 antibody (NBP1-31341) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - HO-1/HMOX1/HSP32 antibody detects HO-1/HMOX1/HSP32 protein at cytoplasm by immunohistochemical analysis.</br>Sample: Paraffin-embedded mouse spleen.</br>HO-1/HMOX1/HSP32 stained by HO-1/HMOX1/HSP32 antibody (NBP1-31341) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HO-1/HMOX1/HSP32 antibody (NBP1-31341) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

<b>Genetic Strategies Validation. </b>Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Wild-type (WT) and Heme Oxygenase 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HO-1/HMOX1/HSP32 antibody (NBP1-31341) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

<b>Orthogonal Strategies Validation. </b>Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HO-1/HMOX1/HSP32 antibody (NBP1-31341) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Sandwich ELISA detection of recombinant full-length HO-1/HMOX1/HSP32 protein using GTX633677 as capture antibody at concentration of 5 ug/mL and NBP1-31341 as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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AF3776

HO-1/HMOX1/HSP32 Antibody [Unconjugated]

(8 Publications)

100 μg

AF3776

HAF017). For additional reference, recombinant human HO-1 and HO-2 (5 ng/lane) were included. A specific band for HO-1/HMOX1/HSP32 was detected at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_lightbox">

Western blot shows lysates of A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse HO-1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3776) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). For additional reference, recombinant human HO-1 and HO-2 (5 ng/lane) were included. A specific band for HO-1/HMOX1/HSP32 was detected at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse HO-1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3776) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human HO-1 and HO-2 (5 ng/lane) were included. A specific band for HO-1/HMOX1/HSP32 was detected at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of A20 mouse B cell lymphoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for HO-1/HMOX1/HSP32 at approximately 37 kDa (as indicated) using 25 µg/mL of Goat Anti-Human/Mouse HO-1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3776) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

HAF017). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HO-1/HMOX1/HSP32 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse HO-1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3776) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3776U-100UG
Alexa Fluor 405	AF3776V-100UG
Alexa Fluor 488	AF3776G-100UG
Alexa Fluor 532	AF3776X-100UG
Alexa Fluor 594	AF3776T-100UG
Alexa Fluor 647	AF3776R-100UG
Alexa Fluor 700	AF3776N-100UG
Alexa Fluor 750	AF3776S-100UG
Alexa Fluor Plus 405	AF3776AFP405-100UG
Alexa Fluor Plus 488	AF3776AFP488-100UG
Alexa Fluor Plus 555	AF3776AFP555-100UG
Alexa Fluor Plus 594	AF3776AFP594-100UG
Alexa Fluor Plus 647	AF3776AFP647-100UG
Alexa Fluor Plus 680	AF3776AFP680-100UG
Allophycocyanin	AF3776A
Biotin	AF3776B
CoraFluor 1	AF3776CL1
DyLight 350	AF3776D
DyLight 405	AF3776E
DyLight 488	AF3776K
DyLight 550	AF3776L
DyLight 594	AF3776M
DyLight 650	AF3776W
DyLight 680	AF3776Y
DyLight 755	AF3776Z
FITC	AF3776F
HRP	AF3776H
Janelia Fluor 525	AF3776JF525
Janelia Fluor 549	AF3776I
Janelia Fluor 646	AF3776J
Janelia Fluor 669	AF3776JF669
PE	AF3776P
PerCP	AF3776C
mFluor Violet 450 SE	AF3776MFV450
mFluor Violet 500 SE	AF3776MFV500
mFluor Violet 610 SE	AF3776MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3776-SP

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MAB3776

HO-1/HMOX1/HSP32 Antibody (412811) [Unconjugated]

(9 Publications)

100 μg

MAB3776

HAF005). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_lightbox">

Western blot shows lysates of A549 human lung carcinoma cell line and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse HO-1/HMOX1/HSP32 Monoclonal Antibody (Catalog # MAB3776) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=

HAF005). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of A549 human lung carcinoma cell line and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse HO-1/HMOX1/HSP32 Monoclonal Antibody (Catalog # MAB3776) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />

HAF005). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

HAF005). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

beta 2-AR and EGFR pathways regulate the ROS metabolism NRF-2 mediated. Evaluation of the ROS metabolism in cancer cells directly obtained from the HNSCC-bearing mice. a Western Blot analysis of Nrf-2 expression in both cytoplasmic and nuclear protein extracts. ICI treatment reduced the Nrf-2 nuclear translocation, in a more evident way if in combination with CTX. b RT-PCR for the HO-1, NQO-1, GCLC, and G6PD gene expression level analysis. The beta 2-AR blockade diminishes the gene expression level of HO-1, with a synergistic effect in combination with CTX, which is able to induce a milder effect by itself. The MEK 1/2 inhibition did not replicate this effect. No significant effects have been observed about the NQO-1 expression. The expression of GCLC and G6PDH is significantly reduced only after treatment with ICI in combination with CTX. c Immunohistochemistry assay on mice tongues engrafted with UMSCC 103 for the HO-1 and NQO1 detection. The ICI and CTX treatments sensibly reduced the expression of HO1, which increases if we combine the drugs. The expression of NQO1 was affected only in mice subjected to the combination of ICI plus CTX. d Cell-ROX assay for the evaluation of the oxidative stress in UMSCC 103 induced by our drugs. With both flow cytometer and e fluorescent microscopy analysis, we observed an increased level of oxidation after treatment with the beta 2-AR inhibitor, as well as with CTX and U0126. The same drugs are even more effective in combination, but this increased effect is counteracted by the KI696. (*P ≤ 0.05; **P ≤ 0.01; ****P ≤ 0.0001 vs. CTR). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37723219), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3776U-100UG
Alexa Fluor 405	FAB3776V-100UG
Alexa Fluor 488	FAB3776G-100UG
Alexa Fluor 532	FAB3776X-100UG
Alexa Fluor 594	FAB3776T-100UG
Alexa Fluor 647	FAB3776R-100UG
Alexa Fluor 700	FAB3776N-100UG
Alexa Fluor 750	FAB3776S-100UG
Alexa Fluor Plus 405	FAB3776AFP405-100UG
Alexa Fluor Plus 488	FAB3776AFP488-100UG
Alexa Fluor Plus 555	FAB3776AFP555-100UG
Alexa Fluor Plus 594	FAB3776AFP594-100UG
Alexa Fluor Plus 647	FAB3776AFP647-100UG
Alexa Fluor Plus 680	FAB3776AFP680-100UG
Allophycocyanin	FAB3776A
Biotin	FAB3776B
CoraFluor 1	FAB3776CL1
DyLight 350	FAB3776D
DyLight 405	FAB3776E
DyLight 488	FAB3776K
DyLight 550	FAB3776L
DyLight 594	FAB3776M
DyLight 650	FAB3776W
DyLight 680	FAB3776Y
DyLight 755	FAB3776Z
FITC	FAB3776F
HRP	FAB3776H
Janelia Fluor 525	FAB3776JF525
Janelia Fluor 549	FAB3776I
Janelia Fluor 646	FAB3776J
Janelia Fluor 669	FAB3776JF669
PE	FAB3776P
PerCP	FAB3776C
mFluor Violet 450 SE	FAB3776MFV450
mFluor Violet 500 SE	FAB3776MFV500
mFluor Violet 610 SE	FAB3776MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB3776-SP

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AF3169

HO-1/HMOX1/HSP32 Antibody [Unconjugated]

(1 Review)

(4 Publications)

100 μg

AF3169

Immunoblot Buffer Group 2." class="big_lightbox">

Western blot shows lysates of C2C12 mouse myoblast cell line, A20 mouse B cell lymphoma cell line, NRK rat normal kidney cells, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse/Rat HO‑1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3169) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated). This experiment was conducted using <a class=NoLineLink href=

Immunoblot Buffer Group 2." alt="Western blot shows lysates of C2C12 mouse myoblast cell line, A20 mouse B cell lymphoma cell line, NRK rat normal kidney cells, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse/Rat HO‑1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3169) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2." class="big_thumb" />

Effect of prodigiosins on the mRNA and protein expression of HO-1 in gastric tissue.Data (means ± SEM of three assays) were normalized to the levels of GAPDH and expressed as fold induction (log2 scale), relative to the level of HO-1 in the control. aP < 0.05, significant difference vs. the health rats; bP < 0.05, significant difference vs. HCl/ethanol-induced rats, Duncan's post-hoc test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31194753), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3169U-100UG
Alexa Fluor 405	AF3169V-100UG
Alexa Fluor 488	AF3169G-100UG
Alexa Fluor 532	AF3169X-100UG
Alexa Fluor 594	AF3169T-100UG
Alexa Fluor 647	AF3169R-100UG
Alexa Fluor 700	AF3169N-100UG
Alexa Fluor 750	AF3169S-100UG
Alexa Fluor Plus 405	AF3169AFP405-100UG
Alexa Fluor Plus 488	AF3169AFP488-100UG
Alexa Fluor Plus 555	AF3169AFP555-100UG
Alexa Fluor Plus 594	AF3169AFP594-100UG
Alexa Fluor Plus 647	AF3169AFP647-100UG
Alexa Fluor Plus 680	AF3169AFP680-100UG
Allophycocyanin	AF3169A
Biotin	AF3169B
CoraFluor 1	AF3169CL1
DyLight 350	AF3169D
DyLight 405	AF3169E
DyLight 488	AF3169K
DyLight 550	AF3169L
DyLight 594	AF3169M
DyLight 650	AF3169W
DyLight 680	AF3169Y
DyLight 755	AF3169Z
FITC	AF3169F
HRP	AF3169H
Janelia Fluor 525	AF3169JF525
Janelia Fluor 549	AF3169I
Janelia Fluor 646	AF3169J
Janelia Fluor 669	AF3169JF669
PE	AF3169P
PerCP	AF3169C
mFluor Violet 450 SE	AF3169MFV450
mFluor Violet 500 SE	AF3169MFV500
mFluor Violet 610 SE	AF3169MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3169-SP

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NBP1-77460

HO-1/HMOX1/HSP32 Antibody - BSA Free

(2 Publications)

0.1 ml

NBP1-77460

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77460AF350
Alexa Fluor 405	NBP1-77460AF405
Alexa Fluor 488	NBP1-77460AF488
Alexa Fluor 532	NBP1-77460AF532
Alexa Fluor 594	NBP1-77460AF594
Alexa Fluor 647	NBP1-77460AF647
Alexa Fluor 700	NBP1-77460AF700
Alexa Fluor 750	NBP1-77460AF750
Alexa Fluor Plus 405	NBP1-77460AFP405
Alexa Fluor Plus 488	NBP1-77460AFP488
Alexa Fluor Plus 555	NBP1-77460AFP555
Alexa Fluor Plus 594	NBP1-77460AFP594
Alexa Fluor Plus 647	NBP1-77460AFP647
Alexa Fluor Plus 680	NBP1-77460AFP680
Allophycocyanin	NBP1-77460APC
Biotin	NBP1-77460B
CoraFluor 1	NBP1-77460CL1
DyLight 350	NBP1-77460UV
DyLight 405	NBP1-77460V
DyLight 488	NBP1-77460G
DyLight 550	NBP1-77460R
DyLight 594	NBP1-77460DL594
DyLight 650	NBP1-77460C
DyLight 680	NBP1-77460FR
DyLight 755	NBP1-77460IR
FITC	NBP1-77460F
HRP	NBP1-77460H
Janelia Fluor 525	NBP1-77460JF525
Janelia Fluor 549	NBP1-77460JF549
Janelia Fluor 646	NBP1-77460JF646
Janelia Fluor 669	NBP1-77460JF669
PE	NBP1-77460PE
PerCP	NBP1-77460PCP
mFluor Violet 450 SE	NBP1-77460MFV450
mFluor Violet 500 SE	NBP1-77460MFV500
mFluor Violet 610 SE	NBP1-77460MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77460SS

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NBP3-15640

HO-1/HMOX1/HSP32 Antibody (7H7W10)

100 ul

NBP3-15640

analysis of extracts of Raw264.7 cells, using Heme Oxygenase 1 (HO-1/HMOX1) antibody (A19062) at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.

<b>Genetic Strategies Validation. </b>Western Blot: Rabbit HO-1/HMOX1/HSP32 mAb (7H7W10) [NBP3-15640] - Analysis of extracts from wild type(WT) and Heme Oxygenase 1 (HO-1/HMOX1) knockout (KO) HeLa cells, using Heme Oxygenase 1 (HO-1/HMOX1) antibody at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Immunohistochemistry: HO-1/HMOX1/HSP32 Antibody (7H7W10) [NBP3-15640] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] HO-1/HMOX1/HSP32 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry: HO-1/HMOX1/HSP32 Antibody (7H7W10) [NBP3-15640] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using [KD Validated] HO-1/HMOX1/HSP32 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry: HO-1/HMOX1/HSP32 Antibody (7H7W10) [NBP3-15640] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KD Validated] HO-1/HMOX1/HSP32 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: HO-1/HMOX1/HSP32 Antibody (7H7W10) [NBP3-15640] - Immunofluorescence analysis of paraffin-embedded human spleen using [KD Validated] HO-1/HMOX1/HSP32 Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP3-25498

HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free

100 ul

NBP3-25498

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Heme Oxygenase 1 antibody [HL1780] detects secreted Heme Oxygenase 1 protein by immunohistochemical analysis. Sample: Paraffin-embedded cat spleen. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

<b>Biological Strategies Validation. </b>Western Blot: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

<b>Biological Strategies Validation. </b>Western Blot: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Heme Oxygenase 1 antibody [HL1780] detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Reactivity:

Hu, Mu, Rt, Fe

Species Glossary

Reactivity: Hu, Mu, Rt, Fe

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-89964

HO-1/HMOX1/HSP32 Antibody - BSA Free

0.1 ml

NBP1-89964

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-89964] - Staining of human skeletal muscle shows shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-89964] - Staining of human Fallopian tube shows shows no positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-89964] - Staining of human prostate shows no positivity in glandular cells as expected.

<b>Orthogonal Strategies Validation. </b>Analysis in human cell lines A-549 and MCF-7 using Anti-HMOX1 antibody. Corresponding HMOX1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-89964-25ul

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NBP1-77459

HO-1/HMOX1/HSP32 Antibody - BSA Free

0.1 ml

NBP1-77459

Reactivity:

Hu, Mu, Pm-Or

Species Glossary

Reactivity: Hu, Mu, Pm-Or

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77459AF350
Alexa Fluor 405	NBP1-77459AF405
Alexa Fluor 488	NBP1-77459AF488
Alexa Fluor 532	NBP1-77459AF532
Alexa Fluor 594	NBP1-77459AF594
Alexa Fluor 647	NBP1-77459AF647
Alexa Fluor 700	NBP1-77459AF700
Alexa Fluor 750	NBP1-77459AF750
Alexa Fluor Plus 405	NBP1-77459AFP405
Alexa Fluor Plus 488	NBP1-77459AFP488
Alexa Fluor Plus 555	NBP1-77459AFP555
Alexa Fluor Plus 594	NBP1-77459AFP594
Alexa Fluor Plus 647	NBP1-77459AFP647
Alexa Fluor Plus 680	NBP1-77459AFP680
Allophycocyanin	NBP1-77459APC
Biotin	NBP1-77459B
CoraFluor 1	NBP1-77459CL1
DyLight 350	NBP1-77459UV
DyLight 405	NBP1-77459V
DyLight 488	NBP1-77459G
DyLight 550	NBP1-77459R
DyLight 594	NBP1-77459DL594
DyLight 650	NBP1-77459C
DyLight 680	NBP1-77459FR
DyLight 755	NBP1-77459IR
FITC	NBP1-77459F
HRP	NBP1-77459H
Janelia Fluor 525	NBP1-77459JF525
Janelia Fluor 549	NBP1-77459JF549
Janelia Fluor 646	NBP1-77459JF646
Janelia Fluor 669	NBP1-77459JF669
PE	NBP1-77459PE
PerCP	NBP1-77459PCP
mFluor Violet 450 SE	NBP1-77459MFV450
mFluor Violet 500 SE	NBP1-77459MFV500
mFluor Violet 610 SE	NBP1-77459MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77459SS

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NBP2-59338

HO-1/HMOX1/HSP32 Antibody (6B8-2F2) - BSA Free

100 μg

NBP2-59338

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-59338AF350
Alexa Fluor 405	NBP2-59338AF405
Alexa Fluor 488	NBP2-59338AF488
Alexa Fluor 532	NBP2-59338AF532
Alexa Fluor 594	NBP2-59338AF594
Alexa Fluor 647	NBP2-59338AF647
Alexa Fluor 700	NBP2-59338AF700
Alexa Fluor 750	NBP2-59338AF750
Alexa Fluor Plus 405	NBP2-59338AFP405
Alexa Fluor Plus 488	NBP2-59338AFP488
Alexa Fluor Plus 555	NBP2-59338AFP555
Alexa Fluor Plus 594	NBP2-59338AFP594
Alexa Fluor Plus 647	NBP2-59338AFP647
Alexa Fluor Plus 680	NBP2-59338AFP680
Allophycocyanin	NBP2-59338APC
Biotin	NBP2-59338B
CoraFluor 1	NBP2-59338CL1
DyLight 350	NBP2-59338UV
DyLight 405	NBP2-59338V
DyLight 488	NBP2-59338G
DyLight 550	NBP2-59338R
DyLight 594	NBP2-59338DL594
DyLight 650	NBP2-59338C
DyLight 680	NBP2-59338FR
DyLight 755	NBP2-59338IR
FITC	NBP2-59338F
HRP	NBP2-59338H
Janelia Fluor 525	NBP2-59338JF525
Janelia Fluor 549	NBP2-59338JF549
Janelia Fluor 646	NBP2-59338JF646
Janelia Fluor 669	NBP2-59338JF669
PE	NBP2-59338PE
PerCP	NBP2-59338PCP
mFluor Violet 450 SE	NBP2-59338MFV450
mFluor Violet 500 SE	NBP2-59338MFV500
mFluor Violet 610 SE	NBP2-59338MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-59338-25ug

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NBP3-21199

HO-1/HMOX1/HSP32 Antibody (CL11661) - BSA Free

100 ul

NBP3-21199

Staining of human prostate shows no positivity in glandular cells as expected.

Staining of human fallopian tube shows no positivity in glandular cells as expected.

Staining of human spleen shows strong granular cytoplasmic positivity in cells in red pulp.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44138AF350
Alexa Fluor 405	NBP3-44138AF405
Alexa Fluor 488	NBP3-44138AF488
Alexa Fluor 532	NBP3-44138AF532
Alexa Fluor 594	NBP3-44138AF594
Alexa Fluor 647	NBP3-44138AF647
Alexa Fluor 700	NBP3-44138AF700
Alexa Fluor 750	NBP3-44138AF750
Alexa Fluor Plus 405	NBP3-44138AFP405
Alexa Fluor Plus 488	NBP3-44138AFP488
Alexa Fluor Plus 555	NBP3-44138AFP555
Alexa Fluor Plus 594	NBP3-44138AFP594
Alexa Fluor Plus 647	NBP3-44138AFP647
Alexa Fluor Plus 680	NBP3-44138AFP680
Allophycocyanin	NBP3-44138APC
Biotin	NBP3-44138B
CoraFluor 1	NBP3-44138CL1
DyLight 350	NBP3-44138UV
DyLight 405	NBP3-44138V
DyLight 488	NBP3-44138G
DyLight 550	NBP3-44138R
DyLight 594	NBP3-44138DL594
DyLight 650	NBP3-44138C
DyLight 680	NBP3-44138FR
DyLight 755	NBP3-44138IR
FITC	NBP3-44138F
HRP	NBP3-44138H
Janelia Fluor 525	NBP3-44138JF525
Janelia Fluor 549	NBP3-44138JF549
Janelia Fluor 646	NBP3-44138JF646
Janelia Fluor 669	NBP3-44138JF669
PE	NBP3-44138PE
PerCP	NBP3-44138PCP
mFluor Violet 450 SE	NBP3-44138MFV450
mFluor Violet 500 SE	NBP3-44138MFV500
mFluor Violet 610 SE	NBP3-44138MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-21199-25ul
Azide and BSA Free	NBP3-44138

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

HO-1/HMOX1/HSP32 Antibodies

HO-1/HMOX1/HSP32 Antibody (HO-1-1) - BSA Free

HO-1/HMOX1/HSP32 Antibody - BSA Free

HO-1/HMOX1/HSP32 Antibody [Unconjugated]

HO-1/HMOX1/HSP32 Antibody (412811) [Unconjugated]

HO-1/HMOX1/HSP32 Antibody [Unconjugated]

HO-1/HMOX1/HSP32 Antibody - BSA Free

HO-1/HMOX1/HSP32 Antibody (7H7W10)

HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free

HO-1/HMOX1/HSP32 Antibody - BSA Free

HO-1/HMOX1/HSP32 Antibody - BSA Free

HO-1/HMOX1/HSP32 Antibody (6B8-2F2) - BSA Free

HO-1/HMOX1/HSP32 Antibody (CL11661) - BSA Free