HO-1/HMOX1/HSP32 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: HO-1/HMOX1/HSP32 Antibody [NBP1-77459] - HepG2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).
Immunohistochemistry: HO-1/HMOX1/HSP32 Antibody [NBP1-77459] - Analysis of heme oxygenase mouse kidney using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.16 mg/ml

HO-1/HMOX1/HSP32 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human Heme Oxygenase 1 protein (between residues 100-150) [UniProt P09601]
Localization
Microsome. Endoplasmic reticulum.
Marker
Oxidative Stress Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HMOX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:25
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This Heme Oxygenase 1 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections.

Reactivity Notes

Human and Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.16 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HO-1/HMOX1/HSP32 Antibody

  • bK286B10
  • EC 1.14.99.3
  • heat shock protein, 32-kD
  • heme oxygenase (decycling) 1
  • HMOX1
  • HO
  • HO1
  • HO-1
  • HO-1heme oxygenase 1
  • HSP32

Background

HO-1 (also know as Heme oxygenase-1, HMOX1, HSP32), a member of the heme oxygenase family, is an enzyme for heme catabolism and cleaves heme to form biliverdin. This inducible protein reacts from hypoxia, cytokines, and oxidative stress. HO-1 is itself an antioxidant that protects cells from ROS (reactive oxygen species) by regulating ROS levels. HO-1 has been shown to inhibit the pathogenesis of immune related inflammatory diseases as well as cardiovascular disease (CVD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for HO-1/HMOX1/HSP32 Antibody (NBP1-77459) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-77459AF405
Alexa Fluor 488 Labeled NBP1-77459AF488
Alexa Fluor 647 Labeled NBP1-77459AF647
Alexa Fluor 700 Labeled NBP1-77459AF700
Biotin Labeled NBP1-77459B
DyLight 405 Labeled NBP1-77459V
DyLight 488 Labeled NBP1-77459G
DyLight 550 Labeled NBP1-77459R
DyLight 650 Labeled NBP1-77459C
FITC Labeled NBP1-77459F

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Bioinformatics

Gene Symbol HMOX1
Entrez
Uniprot