HO-1/HMOX1/HSP32 Antibody

Images

 
Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Wild-type (WT) and Heme Oxygenase 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 ...read more
Immunocytochemistry/ Immunofluorescence: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - A375 cells. Green: Heme Oxygenase 1 protein stained by Heme Oxygenase 1 antibody. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Paraffin-embedded human renal carcinoma diluted at 1:500.
Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - A. mouse brain lysate/extract.12 % SDS-PAGE Heme Oxygenase 1 antibody dilution: 1:1000
Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - analysis of HO-1 in HEK293 cell lysate using anti-HO-1 antibody. Image from verified customer review.
Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody.
Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody diluted at 1:1500.
Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - A. 30 ug IMR32 whole cell lysate/extract B. 30 ug SK-N-AS whole cell lysate/extract 12 % SDS-PAGE Heme Oxygenase 1 antibodydilution: 1:1000
Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Paraffin-embedded rat spleen antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.96 mg/ml
   

Biological Strategies

 

Genetic Strategies

   

Order Details

HO-1/HMOX1/HSP32 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Heme Oxygenase 1. The exact sequence is proprietary.
Localization
Microsome, Endoplasmic reticulum
Marker
Oxidative Stress Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HMOX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-31341 in the following applications:

Publications
Read Publication using
NBP1-31341 in the following applications:

  • WB
    1 publication

Reactivity Notes

Expected cross reactivity based on sequence homology: Canine (85%), Porcine (82%), Bovine (83%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
0.96 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HO-1/HMOX1/HSP32 Antibody

  • bK286B10
  • EC 1.14.99.3
  • heat shock protein, 32-kD
  • heme oxygenase (decycling) 1
  • HMOX1
  • HO
  • HO1
  • HO-1
  • HO-1heme oxygenase 1
  • HSP32

Background

Stressed mammalian cells up-regulate heme oxygenase 1 (HO-1), which catabolizes heme to biliverdin, carbon monoxide, and free iron. Results provide genetic evidence that up-regulation of HO-1 serves as an adaptive mechanism to protect cells from oxidative damage during stress (1). When cells are injured they release their contents, resulting in a local accumulation of free heme proteins and heme. HO-1 plays a crucial role in the inflammatory process during wound healing (2). TNFalpha accelerates inflammatory responses by down-regulating HO-1 expression in human monocytes. TNF antagonists may block this TNF-dependent suppression of HO-1 expression, resulting in an amelioration of inflammation (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HO-1/HMOX1/HSP32 Antibody (NBP1-31341)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Review for HO-1/HMOX1/HSP32 Antibody (NBP1-31341) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-31341:
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WB
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Images Ratings Applications Species Date Details
Western Blot HO-1/HMOX1/HSP32 NBP1-31341
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reviewed by:
Anonymous
WB Human 03/20/2015
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Summary

ApplicationWestern Blot
Sample TestedHEK-293 whole cell lysate
SpeciesHuman

Blocking

Blocking DetailsLicor Blocking buffer

Primary Anitbody

Dilution Ratio1:1000 overnight at 4degrees

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IRdye800
Secondary Concentration1:15000

Details

Detection NotesScan on LicoR Odyssey

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HO-1/HMOX1/HSP32 Antibody (NBP1-31341) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for HO-1/HMOX1/HSP32 Antibody (NBP1-31341)

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Diseases for HO-1/HMOX1/HSP32 Antibody (NBP1-31341)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
03/20/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol HMOX1
Entrez