HO-1/HMOX1/HSP32 Antibody

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Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody diluted at 1:1000. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at endoplasmic reticulum by immunofluorescent analysis. Mock and treated HeLa ...read more
Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Mouse liver. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Analysis of HO-1 in HEK293 cell lysate using anti-HO-1 antibody. WB Image submitted by a verified customer review.
Biological Strategies: Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme ...read more
Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody diluted at 1:1500.
Biological Strategies: Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Nrf2-pathway activation by hybrids: nuclear translocation and targets induction. Protein levels by Western blot. Anti-actin was used ...read more
Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Human renal carcinoma . Heme Oxygenase 1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Rat spleen. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
ELISA: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - ELISA detection of recombinant full-length Heme Oxygenase 1 protein using as capture antibody at concentration of 5 ug/mL and as detection antibody at concentration of 1 ...read more
Biological Strategies: Knockout Validated: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Wild-type (WT) and Heme Oxygenase 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Order Details

HO-1/HMOX1/HSP32 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Heme Oxygenase 1. The exact sequence is proprietary.
Localization
Microsome, Endoplasmic reticulum
Marker
Oxidative Stress Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HMOX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Sandwich ELISA
  • Western Blot 1:500-1:3000
Application Notes
Use in ELISA reported in scientific literature (PMID: 30422019). HO-1/HMOX1/HSP32 antibody validated for WB from a verified customer review. ELISA/ Sandwich Elisa- Assay dependent.
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-31341 in the following applications:

Publications
Read Publications using
NBP1-31341 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (85%), Porcine (82%), Bovine (83%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for HO-1/HMOX1/HSP32 Antibody

  • bK286B10
  • EC 1.14.99.3
  • heat shock protein, 32-kD
  • heme oxygenase (decycling) 1
  • HMOX1
  • HO
  • HO1
  • HO-1
  • HO-1heme oxygenase 1
  • HSP32

Background

Stressed mammalian cells up-regulate heme oxygenase 1 (HO-1), which catabolizes heme to biliverdin, carbon monoxide, and free iron. Results provide genetic evidence that up-regulation of HO-1 serves as an adaptive mechanism to protect cells from oxidative damage during stress (1). When cells are injured they release their contents, resulting in a local accumulation of free heme proteins and heme. HO-1 plays a crucial role in the inflammatory process during wound healing (2). TNFalpha accelerates inflammatory responses by down-regulating HO-1 expression in human monocytes. TNF antagonists may block this TNF-dependent suppression of HO-1 expression, resulting in an amelioration of inflammation (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HO-1/HMOX1/HSP32 Antibody (NBP1-31341)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ELISA, ICC/IF, IHC-P, WB.


Filter By Application
ELISA
(1)
ICC/IF
(1)
IHC-P
(1)
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(8)
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(4)
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(5)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NBP1-31341 Applications Species
Kovacevic S, Ivanov M, Zivotic M Et Al. Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats Antioxidants Jul 22 2021 [PMID: 34439411] (IHC-P) IHC-P
Jing M, Cen Y, Gao F Et Al. Nephroprotective Effects of Tetramethylpyrazine Nitrone TBN in Diabetic Kidney Disease Frontiers in pharmacology Jun 24 2021 [PMID: 34248629] (WB) WB
Wen J, Li S, Zheng C et al. Tetramethylpyrazine nitrone improves motor dysfunction and pathological manifestations by activating the PGC-1 alpha/Nrf2/HO-1 pathway in ALS mice Neuropharmacology Nov 1 2020 [PMID: 33152451] (WB, Mouse) WB Mouse
Serafini M, Catanzaro M, Fagiani F et al. Modulation of Keap1/Nrf2/ARE Signaling Pathway by Curcuma- and Garlic-Derived Hybrids Front. Pharmacol. Jan 28 2020 [PMID: 32047434] (WB, Human) WB Human
Rosini M, Simoni E, Caporaso R et al. Merging memantine and ferulic acid to probe connections between NMDA receptors, oxidative stress and amyloid-beta peptide in Alzheimer's disease Eur J Med Chem Jul 5 2019 [PMID: 31301562] (Human) Human
Phelps CA, Lindsey-Boltz L, Sancar A, Mu D Selenium Deficiency Is Associated with Pro-longevity Mechanisms Cell Rep May 28 2019 [PMID: 31141699] (WB, Mouse) WB Mouse
Mathew G, Sharma A, Pickering RJ et al. A novel synthetic small molecule DMFO targets Nrf2 in modulating proinflammatory/antioxidant mediators to ameliorate inflammation. Free Radic. Res. Nov 13 2018 [PMID: 30422019] (ELISA, Mouse) ELISA Mouse
Moon Su-Jin, Kim Eun-Kyung, Jhun Joo Yeon et al. The active metabolite of leflunomide, A77 1726, attenuates inflammatory arthritis in mice with spontaneous arthritis via induction of heme oxygenase-1. Journal of Translational Medicine 2017 [PMID: 28193225] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Bakkar N, Kousari A, Kovalik T et al. RBM45 modulates the antioxidant response in ALS through interactions with KEAP1 Mol Cell Biol. 2015 May 4 [PMID: 25939382] (WB, Human) WB Human
Pambianchi E, Hagenberg Z, Pecorelli A Et al. Alaskan Bog Blueberry (Vaccinium uliginosum) Extract as an Innovative Topical Approach to Prevent UV-Induced Skin Damage Cosmetics Nov 27 2021 (WB, Human) WB Human
Show All 12 Publications.

Review for HO-1/HMOX1/HSP32 Antibody (NBP1-31341) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-31341:
Filter by Applications
WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot HO-1/HMOX1/HSP32 NBP1-31341
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5
reviewed by:
Nadine Bakkar
WB Human 03/20/2015
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Summary

ApplicationWestern Blot
Sample TestedHEK-293 whole cell lysate
SpeciesHuman

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Recent Reviews

5
5
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1
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Nadine Bakkar
03/20/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol HMOX1