HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free

Western Blot: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Wild-type (WT) and HMOX1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Heme Oxygenase 1 antibody [HL1780] detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Heme Oxygenase 1 antibody [HL1780] detects secreted Heme Oxygenase 1 protein by immunohistochemical analysis. Sample: Paraffin-embedded cat spleen. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Heme Oxygenase 1 antibody [HL1780] detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Ca, Fe
• Applications: WB, IHC
• Clone: HL1780
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free (NBP3-25498) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human HO-1/HMOX1/HSP32
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HMOX1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free

• bK286B10
• EC 1.14.99.3
• heat shock protein, 32-kD
• heme oxygenase (decycling) 1
• HMOX1
• HO
• HO1
• HO-1
• HO-1heme oxygenase 1
• HSP32

Background

Stressed mammalian cells up-regulate heme oxygenase 1 (HO-1), which catabolizes heme to biliverdin, carbon monoxide, and free iron. Results provide genetic evidence that up-regulation of HO-1 serves as an adaptive mechanism to protect cells from oxidative damage during stress (1). When cells are injured they release their contents, resulting in a local accumulation of free heme proteins and heme. HO-1 plays a crucial role in the inflammatory process during wound healing (2). TNFalpha accelerates inflammatory responses by down-regulating HO-1 expression in human monocytes. TNF antagonists may block this TNF-dependent suppression of HO-1 expression, resulting in an amelioration of inflammation (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HMOX1