hnRNP L Antibody (4D11)

Images

 
Western Blot: hnRNP L Antibody (4D11) [NB120-6106] - analysis of hnRNP L in HEK whole cell lysate using anti-hnRNP L antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: hnRNP L Antibody (4D11) [NB120-6106] - hnRNP L antibody was tested in HeLa cells at 5 ug/ml using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) ...read more
Immunohistochemistry-Frozen: hnRNP L Antibody (4D11) [NB120-6106]
Western Blot: hnRNP L Antibody (4D11) [NB120-6106] - Western blot for anti-hnRNP-L on HeLa cell extracts
Western Blot: hnRNP L Antibody (4D11) [NB120-6106] - Whole cell protein from HeLa (lane 1), A431 (lane 2), NIH-3T3 (lane 3) and PC12 (lane 4) was separated by SDS-PAGE and transferred to PVDF membrane. The membrane was ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Am, Bv, Ch, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-Fr, IP
Clone
4D11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

hnRNP L Antibody (4D11) Summary

Immunogen
Human hnRNP proteins from HeLa cells purified by affinity chromatography on ssDNA agarose.
Localization
Nuclear
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HNRNPL
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunohistochemistry-Frozen 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
Control
NIH 3T3 Lysate (NB800-PC8)
A-431 Lysate (NB800-PC12)
HeLa Lysate (NB800-PC1)
PC-12 Lysate (NBP2-13170)
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB120-6106 in the following applications:

Publications
Read Publications using
NB120-6106 in the following applications:

  • 1 publication
  • IP
    5 publications
  • WB
    6 publications

Reactivity Notes

Human, Mouse, Rat, Bovine, Monkey, Chicken and Newt.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for hnRNP L Antibody (4D11)

  • FLJ35509
  • heterogeneous nuclear ribonucleoprotein L
  • hnRNP L
  • hnRNP-L
  • HNRPL

Background

Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (HNRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with HNRNP complexes and along with other HNRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of HNRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions.Strucutrally, hnRPL contains 2 segments of approximately 80 amino acids each which are weakly related to each other and to the ribonucleoprotein consensus sequence-type RNA-binding domains of other HNRNP and snRNP proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Am, Bv, Ch, Pm
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP

Publications for hnRNP L Antibody (NB120-6106)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(1)
IP
(5)
WB
(6)
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Filter By Species
Human
(7)
Mouse
(2)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB120-6106 Applications Species
Yao P, Wu J, Lindner D, Fox PL. Interplay between miR-574-3p and hnRNP L regulates VEGFA mRNA translation and tumorigenesis. Nucleic Acids Res. May 17 2017 [PMID: 28520992] (IP, Mouse) IP Mouse
Li Y, Collins M, An J et al. Immunoprecipitation and mass spectrometry defines an extensive RBM45 protein-protein interaction network Brain Res. Mar 12 2016 [PMID: 26979993] (WB, ICC/IF, IP, Human) WB, ICC/IF, IP Human
Yao P, Potdar AA, Ray PS et al. The HILDA Complex Coordinates a Conditional Switch in the 3'-Untranslated Region of the VEGFA mRNA. PLoS Biol. 2013 Aug [PMID: 23976881] (WB, Human) WB Human
Hung LH, Heiner M, Hui J et al. Diverse roles of hnRNP L in mammalian mRNA processing: a combined microarray and RNAi analysis. RNA. 2008 Feb [PMID: 18073345] (WB, Human) WB Human
Hui J, Stangl K, Lane WS, Bindereif A. HnRNP L stimulates splicing of the eNOS gene by binding to variable-length CA repeats. Nat Struct Biol. 2003 Jan [PMID: 12447348] (WB, Human) WB Human
Soderberg M, Raffalli-Mathieu F, Lang MA. Inflammation modulates the interaction of heterogeneous nuclear ribonucleoprotein (hnRNP) I/polypyrimidine tract binding protein and hnRNP L with the 3'untranslated region of the murine inducible nitric-oxide synthase mRNA. Mol Pharmacol. 2002 Aug [PMID: 12130696] (IP, Mouse) IP Mouse
Shih SC, Claffey KP. Regulation of human vascular endothelial growth factor mRNA stability in hypoxia by heterogeneous nuclear ribonucleoprotein L. J Biol Chem. 1999 Jan 15 [PMID: 9880507] (IP, WB, Human) IP, WB Human
Hahm B, Kim YK, Kim JH et al. Heterogeneous nuclear ribonucleoprotein L interacts with the 3' border of the internal ribosomal entry site of hepatitis C virus. J Virol. 1998 Nov [PMID: 9765422] (WB, Human) WB Human
Liu X, Mertz JE. HnRNP L binds a cis-acting RNA sequence element that enables intron-dependent gene expression. Genes Dev. 1995 Jul 15 [PMID: 7542615] (IP, Human) IP Human

Reviews for hnRNP L Antibody (NB120-6106) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Rat.

Reviews using NB120-6106:
Filter by Applications
IF
(1)
WB
(2)
All Applications
Filter by Species
Human
(2)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence hnRNP L NB120-6106
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reviewed by:
Anonymous
IF Rat 10/22/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedRat Primary Motor Neurons
SpeciesRat

Blocking

Blocking DetailsScytek Super-block

Primary Anitbody

Dilution Ratio1:200, 1h at room temperature

Secondary Antibody

Secondary DescriptionGoat anti-mouse Alexafluor 594
Secondary Concentration1:250

Details

Detection NotesImaged on Zeiss microscope.
Fixation DetailsCells fixed in methanol; no permeabiliation
Wash Description3 washes in PBS, 5 minutes each.
Western Blot hnRNP L NB120-6106
Enlarge
reviewed by:
Anonymous
WB Human 11/14/2014
View

Summary

ApplicationWestern Blot
Sample TestedHEK whole cell lysates
SpeciesHuman

Blocking

Blocking DetailsLicor Blocking buffer

Primary Anitbody

Dilution Ratio1:1000 overnight, at 4C

Secondary Antibody

Secondary DescriptionGoat anti-mouse, IR680
Secondary Concentration1:15000

Details

Detection NotesScan on LicoR Odyssey
 
reviewed by:
Nadine Bakkar
WB Human 08/19/2014
View

Summary

ApplicationWestern Blot
Sample TestedHEK nuclear lysate
SpeciesHuman

Blocking

Blocking DetailsLicoR

Primary Anitbody

Dilution Ratio1:1000 overnight

Secondary Antibody

Secondary Descriptionanti-mouse licor secondary antibody IDdye 800
Secondary Concentration1:15000

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for hnRNP L Antibody (NB120-6106) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB120-6106AF405
Alexa Fluor 488 Labeled NB120-6106AF488
Alexa Fluor 647 Labeled NB120-6106AF647
Alexa Fluor 700 Labeled NB120-6106AF700
DyLight 350 Labeled NB120-6106UV
DyLight 405 Labeled NB120-6106V
DyLight 488 Labeled NB120-6106G
DyLight 680 Labeled NB120-6106FR
DyLight 755 Labeled NB120-6106IR

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Anonymous
10/22/2015
Application: IF
Species: Rat

Anonymous
11/14/2014
Application: WB
Species: Human

 
Nadine Bakkar
08/19/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol HNRNPL
Entrez
Uniprot