hnRNP L Antibody (4D11) - BSA Free

Western Blot: hnRNP L Antibody (4D11) [NB120-6106] - analysis of hnRNP L in HEK whole cell lysate using anti-hnRNP L antibody. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: hnRNP L Antibody (4D11) [NB120-6106] - hnRNP L antibody was tested in HeLa cells at 5 ug/ml using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.

Immunohistochemistry-Frozen: hnRNP L Antibody (4D11) [NB120-6106]

Western Blot: hnRNP L Antibody (4D11) [NB120-6106] - Western blot for anti-hnRNP-L on HeLa cell extracts

Western Blot: hnRNP L Antibody (4D11) [NB120-6106] - Whole cell protein from HeLa (lane 1), A431 (lane 2), NIH-3T3 (lane 3) and PC12 (lane 4) was separated by SDS-PAGE and transferred to PVDF membrane. The membrane was probed with Anti-hnRNP L at 1 ug/ml and detected with an HRP conjugated anti-mouse secondary antibody by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Am, Bv, Ch, Pm
• Applications: WB, Simple Western, ELISA, ICC/IF, IHC, IP
• Clone: 4D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

hnRNP L Antibody (4D11) - BSA Free Summary

• Description: Novus Biologicals Mouse hnRNP L Antibody (4D11) - BSA Free (NB120-6106) is a monoclonal antibody validated for use in IHC, WB, ELISA, ICC/IF, Simple Western and IP. Anti-hnRNP L Antibody: Cited in 10 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human hnRNP proteins from HeLa cells purified by affinity chromatography on ssDNA agarose.
• Localization: Nuclear
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HNRNPL
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Simple Western 1:10 - 1:250
  • Western Blot 1:5000
• Control:

  NIH 3T3 Whole Cell Lysate
  A-431 Whole Cell Lysate
  HeLa Whole Cell Lysate
  PC-12 Whole Cell Lysate

Reactivity Notes

Newt.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for hnRNP L Antibody (4D11) - BSA Free

• FLJ35509
• heterogeneous nuclear ribonucleoprotein L
• hnRNP L
• hnRNP-L
• HNRPL

Background

Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (HNRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with HNRNP complexes and along with other HNRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of HNRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions.Strucutrally, hnRPL contains 2 segments of approximately 80 amino acids each which are weakly related to each other and to the ribonucleoprotein consensus sequence-type RNA-binding domains of other HNRNP and snRNP proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for hnRNP L Antibody (NB120-6106)(11)

Publications using NB120-6106

• Yao P, Wu J, Lindner D, Fox PL. Interplay between miR-574-3p and hnRNP L regulates VEGFA mRNA translation and tumorigenesis. Nucleic Acids Res. 2017-05-17 [PMID: 28520992] (IP, Mouse)
• Li Y, Collins M, An J et al. Immunoprecipitation and mass spectrometry defines an extensive RBM45 protein-protein interaction network Brain Res. 2016-03-12 [PMID: 26979993] (ICC/IF, WB, IP, Human)
• Yao P, Potdar AA, Ray PS et al. The HILDA Complex Coordinates a Conditional Switch in the 3'-Untranslated Region of the VEGFA mRNA. PLoS Biol. 2013-08-01 [PMID: 23976881] (WB, Human)
• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01
• Sun C, Liu M, Chang J et Al. hnRNP L negatively regulates foot-and-mouth disease virus replication through inhibiting viral RNA synthesis by interacting with IRES in 5' untranslated region J. Virol. 2020-03-11 [PMID: 32161169]
• Hung LH, Heiner M, Hui J et al. Diverse roles of hnRNP L in mammalian mRNA processing: a combined microarray and RNAi analysis. RNA. 2008-02-01 [PMID: 18073345] (WB, Human)
• Hui J, Stangl K, Lane WS, Bindereif A. HnRNP L stimulates splicing of the eNOS gene by binding to variable-length CA repeats. Nat Struct Biol. 2003-01-01 [PMID: 12447348] (WB, Human)
• Soderberg M, Raffalli-Mathieu F, Lang MA. Inflammation modulates the interaction of heterogeneous nuclear ribonucleoprotein (hnRNP) I/polypyrimidine tract binding protein and hnRNP L with the 3'untranslated region of the murine inducible nitric-oxide synthase mRNA. Mol Pharmacol. 2002-08-01 [PMID: 12130696] (IP, Mouse)
• Shih SC, Claffey KP. Regulation of human vascular endothelial growth factor mRNA stability in hypoxia by heterogeneous nuclear ribonucleoprotein L. J Biol Chem. 1999-01-15 [PMID: 9880507] (IP, WB, Human)
• Hahm B, Kim YK, Kim JH et al. Heterogeneous nuclear ribonucleoprotein L interacts with the 3' border of the internal ribosomal entry site of hepatitis C virus. J Virol. 1998-11-01 [PMID: 9765422] (WB, Human)
• Liu X, Mertz JE. HnRNP L binds a cis-acting RNA sequence element that enables intron-dependent gene expression. Genes Dev. 1995-07-15 [PMID: 7542615] (IP, Human)

Reviews for hnRNP L Antibody (NB120-6106) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Human, Rat.

reviewed by: Verified Customer IF Rat 10/22/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Rat Primary Motor Neurons
• Species: Rat

reviewed by: Verified Customer WB Human 11/14/2014

Summary

• Application: Western Blot
• Sample Tested: HEK whole cell lysates
• Species: Human

reviewed by: Nadine Bakkar WB Human 08/19/2014

Summary

• Application: Western Blot
• Sample Tested: HEK nuclear lysate
• Species: Human

Bioinformatics

• Gene Symbol: HNRNPL
• Entrez:
  • 3191(Human)
  • 15388(Mouse)
• Uniprot:
  • P14866(Human)
  • Q8R081(Mouse)