Western Blot: NSP 5 alpha 3 alpha Antibody [NB100-517] - Detection of NSP 5 alpha 3 alpha in HeLa whole cell lysate (RIPA) using NB100-517. 0.5ug/ml dilution, 15 second ECL detection.
Immunocytochemistry/ Immunofluorescence: NSP 5 alpha 3 alpha Antibody [NB100-517] - The NSP 5 alpha 3 antibody was tested in Hela cells at a 1:250 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were ...read more
This NSP 5 alpha 3 alpha antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western blot, where a band is seen at ~80 kDa. A second band may be seen at ~60 kDa. The specificity of this band is not known.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
80 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Human. Immunogen has 83% homology with mouse and rat.
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.1% Sodium Azide
Immunogen affinity purified
Alternate Names for NSP 5 alpha 3 alpha Antibody
Nuclear structure protein 5
sperm antigen HCMOGT 1
Sperm antigen HCMOGT-1
sperm antigen with calponin homology and coiled coil domains 1
sperm antigen with calponin homology and coiled-coil domains 1cytokinesis and spindle organization B
sperm antigen with calponin-like and coiled coil domains 1
structure protein NSP5a3a
structure protein NSP5a3b
structure protein NSP5b3a
structure protein NSP5b3b
Nuclear structure protein 5 (NSP5) or NSP 5 alpha 3 alpha (NSP 5a3a) is a novel structural protein which has been found to be over-expressed differently in certain cancer cell lines such as: HT-29, MCF-7, Hela, HN30, Saos-2, CEM, and H23, however, it is barely detectable in normal body tissues except for testis wherein NSP 5a3a is highly expressed. NSP 5a3a interacts with cytoplasmic - nuclear proteins B23 which is known to implicate in multiple cellular processes such as mitosis, cell growth control, and apoptosis, ribosome biogenesis, and rRNA transcription/processing. NSP 5a3a also interacts with hnRNP-L which plays a role in RNA metabolism and rRNA processing. NSP proteins (NSP 5b3a, 5a3b, and 5b3b) have been implicated in several cellular activities such as intracellular trafficking, cellular/subcellular integrity, protein-protein interactions with other structural proteins as well as in protein complex stabilization and scaffolding thus facilitating homo or hetero dimerization of protein complexes. Over-expression of NSP 5a3a has been shown to induce apoptosis in head and neck carcinoma cell lines in particular in HN30 being p53 independent yet involving a novel p73 dependent mechanism wherein NSP 5a3a induces apoptosis through p73-DAXX and TRAF2-TRADD.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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