This Nucleophosmin antibody is useful for Western blot, Immunoprecipitation, Immunofluorescence/Immunocytochemistry, Immunohistochemistry on paraffin-embedded section and Flow Cytometry. In WB a band can be seen at approximately 37 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
37 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Nucleophosmin (NPM; also known as B23, numatrin or NO38) is a highly conserved phosphoprotein that belongs to nucleoplasmin/nucleophosmin family of nuclear chaperones. Expressed ubiquitously with nucleolar localization, NPM shuttles between nucleoli and cytoplasm during cell cycle progression. It binds ribosome to drive ribosome nuclear export and has been associated with nucleolar ribonucleoprotein structures. Chaperonin are the proteins that provide favourable conditions for the correct folding of other proteins for preventing aggregation; and NPM has the ability to acts as chaperonin for the core histones H3, H2B and H4 proteins. NPM stimulates APEX1 endonuclease activity on A/P double-stranded DNA while inhibit the same on A/P single-stranded RNA, and regulate APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA as well as the removal of oxidized rRNA molecules. BRCA2 helps NPM in regulating centrosome duplication, whereas, PLK2 mediated phosphorylation controls the centriole duplication. NPM is implicated in diverse cellular processes including cell proliferation, and regulation of tumor suppressors such as p53/TP53 and ARF.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for NPM1 Antibody (NB110-61646). (Showing 1 - 1 of 1 FAQ).
Does this antibody require antigen retrieval? If so, what antigen retrieval method do you recommend?
For the validation of our Nucleophosmin antibody (NB110-61646), we did not use any customized protocol and in our routine protocol, we use Sodium Citrate buffer pH 6.0 based antigen retrieval method. Please note that formalin fixation as well as paraffin embedding crosslinks the proteins which decreases staining signal by causing conformational changes on the antigens. The inclusion/conditions of antigen retrieval depends primarily upon for how long the samples were in the fixative solution. If the fixation time for samples was more than 4 hours, we would recommend performing Sodium Citrate buffer ph 6.0 based antigen retrieval.
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