NPM1 Antibody

Images

 
Western Blot: Nucleophosmin Antibody [NB110-61646] - Detection of mutant Nucleophosmin in OCI-AML3 lysates.
Immunocytochemistry/ Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in HL-60 cells (negative control).
Immunocytochemistry/ Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in OCI-AML3 cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

NPM1 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal region of human mutant Nucleophosmin (within residues 250-294). [Accession# AAW67752]
Localization
Nucleophosmin typically resides in the nucleus. However, in patients with acute myelogenous leukemia (AML) it mutates and localizes to the cytoplasm.
Specificity
This antibody is specific for the mutant form of Nucleophosmin.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NPM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation 1:50
Application Notes
This Nucleophosmin antibody is useful for Western blot, Immunoprecipitation, Immunofluorescence/Immunocytochemistry, Immunohistochemistry on paraffin-embedded section and Flow Cytometry. In WB a band can be seen at approximately 37 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
NPM1 Lysate (NBL1-13748)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NPM1 Antibody

  • B23
  • MGC104254
  • NPM1
  • NPMnucleophosmin/nucleoplasmin family, member 1
  • Nucleolar phosphoprotein B23
  • Nucleolar protein NO38
  • nucleophosmin (nucleolar phosphoprotein B23, numatrin)
  • Nucleophosmin
  • Numatrin

Background

Nucleophosmin (NPM; also known as B23, numatrin or NO38) is a highly conserved phosphoprotein that belongs to nucleoplasmin/nucleophosmin family of nuclear chaperones. Expressed ubiquitously with nucleolar localization, NPM shuttles between nucleoli and cytoplasm during cell cycle progression. It binds ribosome to drive ribosome nuclear export and has been associated with nucleolar ribonucleoprotein structures. Chaperonin are the proteins that provide favourable conditions for the correct folding of other proteins for preventing aggregation; and NPM has the ability to acts as chaperonin for the core histones H3, H2B and H4 proteins. NPM stimulates APEX1 endonuclease activity on A/P double-stranded DNA while inhibit the same on A/P single-stranded RNA, and regulate APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA as well as the removal of oxidized rRNA molecules. BRCA2 helps NPM in regulating centrosome duplication, whereas, PLK2 mediated phosphorylation controls the centriole duplication. NPM is implicated in diverse cellular processes including cell proliferation, and regulation of tumor suppressors such as p53/TP53 and ARF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for NPM1 Antibody (NB110-61646): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NPM1 Antibody (NB110-61646). (Showing 1 - 1 of 1 FAQ).

  1. Does this antibody require antigen retrieval? If so, what antigen retrieval method do you recommend?
    • For the validation of our Nucleophosmin antibody (NB110-61646), we did not use any customized protocol and in our routine protocol, we use Sodium Citrate buffer pH 6.0 based antigen retrieval method. Please note that formalin fixation as well as paraffin embedding crosslinks the proteins which decreases staining signal by causing conformational changes on the antigens. The inclusion/conditions of antigen retrieval depends primarily upon for how long the samples were in the fixative solution. If the fixation time for samples was more than 4 hours, we would recommend performing Sodium Citrate buffer ph 6.0 based antigen retrieval.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-61646AF405
Alexa Fluor 488 Labeled NB110-61646AF488
Alexa Fluor 647 Labeled NB110-61646AF647
Alexa Fluor 700 Labeled NB110-61646AF700
Biotin Labeled NB110-61646B
DyLight 405 Labeled NB110-61646V
DyLight 488 Labeled NB110-61646G
DyLight 550 Labeled NB110-61646R
DyLight 650 Labeled NB110-61646C
FITC Labeled NB110-61646F
HRP Labeled NB110-61646H
PE Labeled NB110-61646PE

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Bioinformatics

Gene Symbol NPM1
Entrez