NPM1 Antibody - BSA Free

Western Blot: Nucleophosmin Antibody [NB110-61646] - Detection of mutant Nucleophosmin in OCI-AML3 lysates.

Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in HL-60 cells (negative control).

Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in OCI-AML3 cells.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

NPM1 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to a C-terminal region of human mutant Nucleophosmin (within residues 250-294). [Accession# AAW67752]
• Localization: Nucleophosmin typically resides in the nucleus. However, in patients with acute myelogenous leukemia (AML) it mutates and localizes to the cytoplasm.
• Specificity: This antibody is specific for the mutant form of Nucleophosmin.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NPM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 1:50 - 1:100
  • Immunohistochemistry-Paraffin 1:50 - 1:100
  • Immunoprecipitation 1:50
  • Western Blot 1:500
• Application Notes: In WB a band can be seen at ~37 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NPM1 Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30214626).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NPM1 Antibody - BSA Free

• B23
• MGC104254
• NPM1
• NPMnucleophosmin/nucleoplasmin family, member 1
• Nucleolar phosphoprotein B23
• Nucleolar protein NO38
• nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Nucleophosmin
• Numatrin

Background

Nucleophosmin (NPM; also known as B23, numatrin or NO38) is a highly conserved phosphoprotein that belongs to nucleoplasmin/nucleophosmin family of nuclear chaperones. Expressed ubiquitously with nucleolar localization, NPM shuttles between nucleoli and cytoplasm during cell cycle progression. It binds ribosome to drive ribosome nuclear export and has been associated with nucleolar ribonucleoprotein structures. Chaperonin are the proteins that provide favourable conditions for the correct folding of other proteins for preventing aggregation; and NPM has the ability to acts as chaperonin for the core histones H3, H2B and H4 proteins. NPM stimulates APEX1 endonuclease activity on A/P double-stranded DNA while inhibit the same on A/P single-stranded RNA, and regulate APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA as well as the removal of oxidized rRNA molecules. BRCA2 helps NPM in regulating centrosome duplication, whereas, PLK2 mediated phosphorylation controls the centriole duplication. NPM is implicated in diverse cellular processes including cell proliferation, and regulation of tumor suppressors such as p53/TP53 and ARF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NPM1 Antibody (NB110-61646)(4)

Publications using NB110-61646

• Wang XQD, Fan D, Han Q et al. Mutant NPM1 Hijacks Transcriptional Hubs to Maintain Pathogenic Gene Programs in Acute Myeloid Leukemia Cancer discovery 2023-03-01 [PMID: 36455589] (WB, IP, ICC/IF)
  
  Details:
  Cut & Run
• Li Y, Ivica NA, Dong T et al. MFSD7C switches mitochondrial ATP synthesis to thermogenesis in response to heme Nat Commun 2020-09-24 [PMID: 32973183] (WB, Mouse)
• El-Gamal RAE, Hashem AE, Habashy DM et al. Flow cytometry in detection of Nucleophosmin 1 mutation in acute myeloid leukemia patients: A reproducible tertiary hospital experience Int J Lab Hematol 2020-08-27 [PMID: 32856429]
  
  Details:
  Citation using the DyLight 488 format of this antibody.
• Zhang W, Zhao C, Zhao J et al. Inactivation of PBX3 and HOXA9 by down-regulating H3K79 methylation represses NPM1-mutated leukemic cell survival Theranostics 2018-08-12 [PMID: 30214626] (WB, ICC/IF, Mouse)

Reviews for NPM1 Antibody (NB110-61646) (2)

Average Rating: 3

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 03/16/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: fibroblasts
• Species: Human
• Lot: E-1

Comments

• Comments: There was strong signal, but the signal was strong in the both the nucleus and the cytoplasm. 1:100 dilution was used, so perhaps using more diluted antibody would help.

reviewed by: Verified Customer WB Human 01/18/2018

Summary

• Application: Western Blot
• Sample Tested: Human lymphocytes
• Species: Human
• Lot: E-1

Comments

• Comments: 1:500 16 hours 4C in 5% milk-TBS/T
  1 minute exposure, 25ug protein.

FAQs for NPM1 Antibody (NB110-61646). (Showing 1 - 1 of 1 FAQs).

1. Does this antibody require antigen retrieval? If so, what antigen retrieval method do you recommend?
   • For the validation of our Nucleophosmin antibody (NB110-61646), we did not use any customized protocol and in our routine protocol, we use Sodium Citrate buffer pH 6.0 based antigen retrieval method. Please note that formalin fixation as well as paraffin embedding crosslinks the proteins which decreases staining signal by causing conformational changes on the antigens. The inclusion/conditions of antigen retrieval depends primarily upon for how long the samples were in the fixative solution. If the fixation time for samples was more than 4 hours, we would recommend performing Sodium Citrate buffer ph 6.0 based antigen retrieval.

Bioinformatics

• Gene Symbol: NPM1