APE Antibody (13B8E5C2) - BSA Free

Images

 
Western Blot: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, ...read more
Genetic Strategies: Knockdown Validated: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - Repression of p21 by APE1 in p53-null cells and effect of ectopic p53 in this ...read more
Immunocytochemistry/ Immunofluorescence: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - HeLa cells stained NB100-116 (Green) detected with DyLight Fluor 488 conjugated anti-rabbit ...read more
Immunohistochemistry: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - Immunohistochemical staining of APE1. A. Nuclear staining in the luminal epithelium of normal breast ducts and ...read more
Chromatin Immunoprecipitation (ChIP): APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - Association of p53 and APE1 on p53-binding sites in p21 promoter. Re-ChIP analysis (first IP ...read more
EBNA1 promotes the upregulation of oxidative DNA damage repair pathways in EBV converted cell lines and EBV positive BLs. a Representative western blots illustrating the expression of MTH1, OGG1, and MUTYH in pairs of ...read more
Western blot images (A) and quantifications (B–G) of cell lysates from C2C12 myotubes with control or UCHL1 siRNA knockdown for perilipin 2 (B), perilipin 5 (C), CD36 (D), HSL (E), MAGL (F), and SDHB (G). n = 4 per ...read more
BDNF lowered the mechanical withdrawal threshold further and promoted activation of astrocytes and microglia, and enhanced the p38/JNK pathway to aggravate the release of IL-1 beta and TNF-alpha in the SDH of ...read more
Primary human grade IV glioblastoma xenotransplant.(A) Dynamic PET image (coronal) of advanced tumor in the right hemisphere of the brain with [18F]PBR06 uptake confined primarily to the tumor. Arrows indicate tumor and ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ELISA, Func, GS, ICC/IF, IHC, IP, WB, ChIP, KD
Clone
13B8E5C2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

APE Antibody (13B8E5C2) - BSA Free Summary

Immunogen
Purified human APE1 [Uniprot: P27695]
Epitope
The epitope is within amino acids 80-100.
Localization
In immunohistochemical experiments punctate nuclear staining is observed in most cell types. Cytoplasmic staining may be seen in hepatocytes, large neurons and granule cells.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
APEX1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • ELISA reported in scientific literature (PMID 21769563)
  • Gel Super Shift Assays reported in scientific literature
  • Immunoblotting reported in scientific literature (PMID 27608656)
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10 - 1:500. Use reported in scientific literature (PMID 35286386)
  • Knockdown Validated
  • Proximity Ligation Assay reported in scientific literature (PMID 27808278)
  • Simple Western 1:25
  • Western Blot 1:100-1:2000
Application Notes
In Western blot, this antibody detects a single band at 37 kDa. In IHC, it can be competitively inhibited from recognizing the APE1 antigen in tissues using APE1 protein. It can also be used on frozen and fixed-paraffin sections and cytospin preps. In IHC-P, staining was observed in the nucleus of a human breast cancer xenograft. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Antagonist
Control
HeLa Whole Cell Lysate
APE Overexpression Lysate
Reviewed Applications
Read 3 Reviews rated 5
using
NB100-116 in the following applications:

Publications
Read Publications using
NB100-116 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for APE Antibody (13B8E5C2) - BSA Free

  • AP endonuclease 1
  • APE
  • APE1
  • APE-1
  • APEAPEX nuclease (multifunctional DNA repair enzyme)
  • APENAP endonuclease class I
  • APEX deoxyribonuclease (apurinic or apyrimidinic)
  • APEX nuclease (multifunctional DNA repair enzyme) 1
  • APEX nuclease
  • APEX1
  • Apurinic-apyrimidinic endonuclease 1
  • APXAP lyase
  • DNA-(apurinic or apyrimidinic site) lyase
  • EC 3.1
  • EC 4.2.99.18
  • HAP1apurinic/apyrimidinic (abasic) endonuclease
  • multifunctional DNA repair enzyme
  • protein REF-1
  • redox factor 1
  • Redox factor-1
  • REF1 apurinic/apyrimidinic exonuclease
  • REF-1

Background

The mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is responsible for the repair of AP sites in DNA. In addition, this enzyme functions as a redox factor facilitating the DNA binding capability of FOS, JUN, NfkB, HIF-1 alpha, Pax-5, Pax-8 and other transcription factors. APE/ref-1 has also been shown to control p53 activity through redox alteration. APE is linked to apoptosis, associated with thioredoxin, and altered levels of APE/ref-1 have been found in some cancers. APE appears to form a unique link between the DNA base excision pathway, oxidative signaling, transcription regulation, cancer and cell-cycle control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APE Antibody (NB100-116)(105)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate.

We have publications tested in 9 applications: Chemotaxis, EMSA, GS, ICC/IF, IF/IHC, IHC-P, IP, PLA, WB.


Filter By Application
Chemotaxis
(8)
EMSA
(1)
GS
(1)
ICC/IF
(15)
IF/IHC
(10)
IHC-P
(11)
IP
(10)
PLA
(1)
WB
(48)
All Applications
Filter By Species
Human
(61)
Mouse
(21)
Rat
(7)
Primate
(1)
All Species
Showing Publications 1 - 10 of 105. Show All 105 Publications.
Publications using NB100-116 Applications Species
Ito M, Ducasa GM, Molina JD et al. ABCA1 deficiency contributes to podocyte pyroptosis priming via the APE1/IRF1 axis in diabetic kidney disease Scientific reports 2023-06-14 [PMID: 37316538] (WB, Mouse)

Details:
1:500 dilution, fresh sections used
WB Mouse
Chen YH, Kuo YY, You YQ et al. Endonuclease VIII-like 1 deficiency potentiates nigrostriatal dopaminergic neuron degeneration in a male mouse model of Parkinson's disease Journal of neurochemistry 2023-02-24 [PMID: 36840377]
Latino D, Chieffi Baccari G, Di Fiore MM et al. Autophagy and mitochondrial damage in the testis of high-fat diet fed rats General and comparative endocrinology 2022-08-13 [PMID: 35973585] (WB, Rat)

Details:
Dilution used for WB 1:1500
WB Rat
Chieffi Baccari G, Falvo S, Di Fiore MM et al. High-fat diet affects autophagy and mitochondrial compartment in rat Harderian gland Journal of experimental zoology. Part A, Ecological and integrative physiology 2022-08-04 [PMID: 35927786]
Rios-Covian D, Butcher LD, Ablack AL et al. A novel hypomorphic Apex1 mouse model implicates apurinic-apyrimidinic endonuclease 1 in oxidative DNA damage repair in gastric epithelial cells Antioxidants & redox signaling 2022-06-25 [PMID: 35754343] (WB) WB
Pramanik S, Chen Y, Song H et al. The human AP-endonuclease 1 (APE1) is a DNA G-quadruplex structure binding protein and regulates KRAS expression in pancreatic ductal adenocarcinoma cells Nucleic acids research 2022-03-14 [PMID: 35286386] (IP, WB, ICC/IF, Human) IP, WB, ICC/IF Human
Hernandez JOR Hepatic and Systemic Effects of Angiotensin II Type 1 (AT1) Receptor Inhibition on the Oxidative and Inflammatory Response in Healthy Rhesus Monkeys (Macaca mulatta) During Aging Thesis 2020-01-01
Song H, Xi S, Chen Y Et Al. Histone chaperone FACT complex inhibitor CBL0137 interferes with DNA damage repair and enhances sensitivity of medulloblastoma to chemotherapy and radiation Cancer letters 2021-07-14 [PMID: 34271103] (IP, WB, ICC/IF) IP, WB, ICC/IF
Shen J, Varshney D, Simeone A et al. Promoter G-quadruplex folding precedes transcription and is controlled by chromatin Genome biology 2021-05-07 [PMID: 33962653] (WB, Human) WB Human
Gampala S, Shah F, Zhang C et al. Exploring transcriptional regulators Ref-1 and STAT3 as therapeutic targets in malignant peripheral nerve sheath tumours British journal of cancer 2021-03-03 [PMID: 33658640] (IHC-P, WB) IHC-P, WB
Show All 105 Publications.

Reviews for APE Antibody (NB100-116) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-116:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Luiz Alexandre Magno
WB Mouse 07/15/2018
View

Summary

ApplicationWestern Blot
Sample TestedBlood mononuclear cells (PBMCs)
SpeciesMouse
LotC-3
  5
reviewed by:
Lisa Lirussi
WB Human 03/28/2014
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
  5
reviewed by:
Verified Customer
WB Human 09/08/2009
View

Summary

ApplicationWestern Blot
Sample TestedHuman melanoma cells, Sample Amount: 25ug
SpeciesHuman
LotB7

Product General Protocols

View specific protocols for APE Antibody (NB100-116): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for APE Antibody (NB100-116). (Showing 1 - 2 of 2 FAQs).

  1. I have problems performing IF on HeLa cells with this antibody (NB100-116). I have a very weird pattern of staining. Is there a special protocol I need to follow?
    • Please see the following link for our product specific protocol for NB100-116.
  2. Today I have a customer who is having some difficulty with NB100-116 in his IP experiments. I have sent him a link to a journal that references use of the antibody in IP, but he has now asked if the antibody recognizes the reduced form of APE1 and not the oxidized form. Do you have any information available?
    • It seems that in Western blot, Novus as well as our customers use reducing conditions, as is standard. I have also looked at our publications using this antibody in IP and nothing suggests that it needs to be used in reducing conditions for IP. It would seem that it recognizes both forms and should work in IP in most standard IP protocols.
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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

 
Luiz Alexandre Magno
07/15/2018
Application: WB
Species: Mouse

 
Lisa Lirussi
03/28/2014
Application: WB
Species: Human

 
Verified Customer
09/08/2009
Application: WB
Species: Human

Bioinformatics

Gene Symbol APEX1
Entrez
Uniprot