APE Antibody (13B8E5C2)

Images

 
Western Blot: APE Antibody (13B8E5C2) [NB100-116] - Ovarian Cancer cell lines.
Immunocytochemistry/ Immunofluorescence: APE Antibody (13B8E5C2) [NB100-116] - HeLa cells stained NB100-116 (Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are ...read more
Immunohistochemistry-Paraffin: APE Antibody (13B8E5C2) [NB100-116] - APE1 antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: APE Antibody (13B8E5C2) [NB100-116] - Immunocytochemical detection of APE-ref-1 in breast cancer cell line MDS231.
Simple Western: APE Antibody (13B8E5C2) [NB100-116] - Simple Western lane view shows a specific band for APE1 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ChIP, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
13B8E5C2
Clonality
Monoclonal
Host
Mouse

Order Details

APE Antibody (13B8E5C2) Summary

Immunogen
Purified human APE1 [UniProt# P27695]
Epitope
The epitope is within amino acids 80-100.
Localization
In immunohistochemical experiments punctate nuclear staining is observed in most cell types. Cytoplasmic staining may be seen in hepatocytes, large neurons and granule cells.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
APEX1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
Ascites with PBS
Preservative
0.05% Sodium Azide
Purity
Unpurified

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Simple Western 1:25
  • Chromatin Immunoprecipitation 1:10-1:500
  • Gel Super Shift Assays
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
This APE1 (13B8E5C2) antibody is useful for Western Blot, Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Gel Super Shift Assays was reported in scientific literature. In WB this antibody detects a single band at 37 kDa. In IHC, it can be competitively inhibited from recognizing the APE1 antigen in tissues using APE1 protein. It can also be used on frozen and fixed-paraffin sections and cytospin preps. In IHC-P, staining was observed in the nucleus of a human breast cancer xenograft. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Antagonist
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-116 in the following applications:

Publications
Read Publications using
NB100-116 in the following applications:

  • 5 publications
  • 1 publication
  • 1 publication
  • GS
    1 publication
  • 10 publications
  • IHC
    6 publications
  • 8 publications
  • IP
    7 publications
  • WB
    31 publications

Alternate Names for APE Antibody (13B8E5C2)

  • AP endonuclease 1
  • APE
  • APE1
  • APE-1
  • APEAPEX nuclease (multifunctional DNA repair enzyme)
  • APENAP endonuclease class I
  • APEX deoxyribonuclease (apurinic or apyrimidinic)
  • APEX nuclease (multifunctional DNA repair enzyme) 1
  • APEX nuclease
  • APEX1
  • Apurinic-apyrimidinic endonuclease 1
  • APXAP lyase
  • DNA-(apurinic or apyrimidinic site) lyase
  • EC 3.1
  • EC 4.2.99.18
  • HAP1apurinic/apyrimidinic (abasic) endonuclease
  • multifunctional DNA repair enzyme
  • protein REF-1
  • redox factor 1
  • Redox factor-1
  • REF1 apurinic/apyrimidinic exonuclease
  • REF-1

Background

The mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is responsible for the repair of AP sites in DNA. In addition, this enzyme functions as a redox factor facilitating the DNA binding capability of FOS, JUN, NfkB, HIF-1 alpha, Pax-5, Pax-8 and other transcription factors. APE/ref-1 has also been shown to control p53 activity through redox alteration. APE is linked to apoptosis, associated with thioredoxin, and altered levels of APE/ref-1 have been found in some cancers. APE appears to form a unique link between the DNA base excision pathway, oxidative signaling, transcription regulation, cancer and cell-cycle control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ChIP, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for APE Antibody (NB100-116)(69)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate.

We have publications tested in 9 applications: ChIP, ChiP, EMSA, GS, ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ChIP
(5)
ChiP
(1)
EMSA
(1)
GS
(1)
ICC/IF
(10)
IHC
(6)
IHC-P
(8)
IP
(7)
WB
(31)
All Applications
Filter By Species
Human
(45)
Mouse
(15)
Rat
(6)
Primate
(1)
All Species
Showing Publications 1 - 10 of 69. Show All 69 Publications.
Publications using NB100-116 Applications Species
Akhter N, Takeda Y, Nara H et al. Apurinic/apyrimidinic endonuclease 1/redox factor-1 (Ape1/Ref-1) modulates antigen presenting cell-mediated T helper cell type 1 responses J. Biol. Chem. Sep 16 2016 12:00AM [PMID:27637330] (WB, Mouse) WB Mouse
Lee YR, Lim JS, Shin JH et al. Altered Secretory Activity of APE1/Ref-1 D148E Variants Identified in Human Patients With Bladder Cancer. Int Neurourol J. May 1 2016 12:00AM [PMID:27230458] (WB, Human) WB Human
Wang H, Cai S, Bailey BJ et al. Combination therapy in a xenograft model of glioblastoma: enhancement of the antitumor activity of temozolomide by an MDM2 antagonist. J. Neurosurg. May 13 2016 12:00AM [PMID:27177180]
Moreli JB, Santos JH, Lorenzon-Ojea AR et al. Hyperglycemia Differentially Affects Maternal and Fetal DNA Integrity and DNA Damage Response Int. J. Biol. Sci. Mar 29 2016 12:00AM [PMID:27019630]
Ahuja AK, Jodkowska K, Teloni F et al. A short G1 phase imposes constitutive replication stress and fork remodelling in mouse embryonic stem cells. Nat Commun. 2016 Feb 15 [PMID:26876348] (WB, Mouse) WB Mouse
Aamann MD, Norregaard R, Kristensen ML et al. Unilateral ureteral obstruction induces DNA repair by APE1. Am. J. Physiol. Renal Physiol. 2015 Nov 25 [PMID:26608791] (IHC-P, Rat) IHC-P Rat
Pojo M, Goncalves CS, Xavier-Magalhaes A et al. A transcriptomic signature mediated by HOXA9 promotes human glioblastoma initiation, aggressiveness and resistance to temozolomide. Oncotarget. 2015 Feb 20 [PMID:25762636] (WB, Human) WB Human
Yang S, Zhang J, Zhang Y et al. KDM1A triggers androgen-induced miRNA transcription via H3K4me2 demethylation and DNA oxidation Prostate 2015 Mar 01 [PMID:25728837] (ChiP, Human) ChiP Human
Fang Q, Inanc B, Schamus S et al. HSP90 regulates DnA repair via the interaction between XRCC1 and DnA polymerase B. Nat Commun. 2014 Nov 26 [PMID:25423885] (WB) WB
Woo J, Park H, Sung SH et al. Prognostic Value of Human Apurinic/Apyrimidinic Endonuclease 1 (APE1) Expression in Breast Cancer PLoS ONE. 2014 Jun 11 [PMID:24914806] (IHC-P, Human) IHC-P Human
Show All 69 Publications.

Reviews for APE Antibody (NB100-116) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 review tested in 1 application: WB.

Reviews using NB100-116:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Lisa Lirussi
WB Human 03/28/2014
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman

Blocking

Blocking Details5% milk in PBS-T for 1hr at RT

Primary Anitbody

Dilution Ratio1:2000 for 2hr at RT in blocking solution

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG
Secondary Concentration1:10000

Details

Detection NotesFluorescence
 
reviewed by:
Anonymous
WB Human 09/08/2009
View

Summary

ApplicationWestern Blot
Sample TestedHuman melanoma cells, Sample Amount: 25ug
SpeciesHuman
LotB7

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat milk, Blocking Time: 2 hours, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1/3000, Incubation Dilution Buffer: 5% non-fat milk, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: Santa Cruz, Secondary Ab Dilution Ratio: 1/1000

Details

Detection NotesDetection Method: substrate hrp, Exposure Time: 5 seconds, Specific Bands: 39 kDa

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for APE Antibody (NB100-116). (Showing 1 - 2 of 2 FAQs).

  1. I have problems performing IF on HeLa cells with this antibody (NB100-116). I have a very weird pattern of staining. Is there a special protocol I need to follow?
    • Please see the following link for our product specific protocol for NB100-116.
  2. Today I have a customer who is having some difficulty with NB100-116 in his IP experiments. I have sent him a link to a journal that references use of the antibody in IP, but he has now asked if the antibody recognizes the reduced form of APE1 and not the oxidized form. Do you have any information available?
    • It seems that in Western blot, Novus as well as our customers use reducing conditions, as is standard. I have also looked at our publications using this antibody in IP and nothing suggests that it needs to be used in reducing conditions for IP. It would seem that it recognizes both forms and should work in IP in most standard IP protocols.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol APEX1
Entrez
Uniprot

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