Immunogen | Purified human APE1 [Uniprot: P27695] |
Epitope | The epitope is within amino acids 80-100. |
Localization | In immunohistochemical experiments punctate nuclear staining is observed in most cell types. Cytoplasmic staining may be seen in hepatocytes, large neurons and granule cells. |
Isotype | IgG2b |
Clonality | Monoclonal |
Host | Mouse |
Gene | APEX1 |
Purity | Protein G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Use in IP reported in scientific literature (PMID:35286386) Gel Super Shift Assays was reported in scientific literature. In Western blot, this antibody detects a single band at 37 kDa. In IHC, it can be competitively inhibited from recognizing the APE1 antigen in tissues using APE1 protein. It can also be used on frozen and fixed-paraffin sections and cytospin preps. In IHC-P, staining was observed in the nucleus of a human breast cancer xenograft. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in Proximity Ligation Assay reported in scientific literature (PMID: 27808278). Use in immunoblotting reported in scientific literature (PMID: 27608656). Use in ELISA reported in scientific literature (PMID: 21769563).. |
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Theoretical MW | 37 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | PBS |
Preservative | 0.02% Sodium Azide |
Concentration | 1.0 mg/ml |
Purity | Protein G purified |
Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
WB | Mouse | 07/15/2018 |
Summary
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reviewed by:
Verified Customer |
WB | Human | 03/28/2014 |
Summary
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reviewed by:
System Admin |
WB | Human | 09/08/2009 |
Summary
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Secondary Antibodies |
Isotype Controls |
Diseases for APE Antibody (NB100-116)Discover more about diseases related to APE Antibody (NB100-116).
| Pathways for APE Antibody (NB100-116)View related products by pathway.
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PTMs for APE Antibody (NB100-116)Learn more about PTMs related to APE Antibody (NB100-116).
| Research Areas for APE Antibody (NB100-116)Find related products by research area.
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Verified Customer 07/15/2018 |
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Application: | WB | |
Species: | Mouse |
Verified Customer 03/28/2014 |
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Application: | WB | |
Species: | Human |
System Admin 09/08/2009 |
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Application: | WB | |
Species: | Human |