APE Antibody (13B8E5C2)

Western Blot: APE Antibody (13B8E5C2) [NB100-116] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-APE-1 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

Immunocytochemistry/Immunofluorescence: APE Antibody (13B8E5C2) [NB100-116] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-APE (13B8E5C2) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: APE Antibody (13B8E5C2) [NB100-116] - APE1 antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain.

Western Blot: APE Antibody (13B8E5C2) [NB100-116] - Ovarian Cancer cell lines.

Immunocytochemistry/Immunofluorescence: APE Antibody (13B8E5C2) [NB100-116] - Immunocytochemical detection of APE-ref-1 in breast cancer cell line MDA MB 231.

Immunocytochemistry/Immunofluorescence: APE Antibody (13B8E5C2) [NB100-116] - HeLa cells stained NB100-116 (Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).

Simple Western: APE Antibody (13B8E5C2) [NB100-116] - Simple Western lane view shows a specific band for APE1 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Simple Western, ChIP, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA
• Clone: 13B8E5C2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

APE Antibody (13B8E5C2) Summary

• Immunogen: Purified human APE1 [UniProt# P27695]
• Epitope: The epitope is within amino acids 80-100.
• Localization: In immunohistochemical experiments punctate nuclear staining is observed in most cell types. Cytoplasmic staining may be seen in hepatocytes, large neurons and granule cells.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: APEX1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • Simple Western 1:25
  • Chromatin Immunoprecipitation 1:10-1:500
  • Gel Super Shift Assays
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
  • Proximity Ligation Assay
• Application Notes: This APE1 (13B8E5C2) antibody is useful for Western Blot, Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Gel Super Shift Assays was reported in scientific literature. In WB this antibody detects a single band at 37 kDa. In IHC, it can be competitively inhibited from recognizing the APE1 antigen in tissues using APE1 protein. It can also be used on frozen and fixed-paraffin sections and cytospin preps. In IHC-P, staining was observed in the nucleus of a human breast cancer xenograft. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Proximity Ligation Assay reported in scientific literature (PMID 27808278).
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Antagonist:
  • APE1 Redox Inhibitor
• Positive Control:

  HeLa Lysate (NB800-PC1)
  APE Lysate (NBL1-07597)
  APE Lysate (NBP2-65552)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for APE Antibody (13B8E5C2)

• AP endonuclease 1
• APE
• APE1
• APE-1
• APEAPEX nuclease (multifunctional DNA repair enzyme)
• APENAP endonuclease class I
• APEX deoxyribonuclease (apurinic or apyrimidinic)
• APEX nuclease (multifunctional DNA repair enzyme) 1
• APEX nuclease
• APEX1
• Apurinic-apyrimidinic endonuclease 1
• APXAP lyase
• DNA-(apurinic or apyrimidinic site) lyase
• EC 3.1
• EC 4.2.99.18
• HAP1apurinic/apyrimidinic (abasic) endonuclease
• multifunctional DNA repair enzyme
• protein REF-1
• redox factor 1
• Redox factor-1
• REF1 apurinic/apyrimidinic exonuclease
• REF-1

Background

The mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is responsible for the repair of AP sites in DNA. In addition, this enzyme functions as a redox factor facilitating the DNA binding capability of FOS, JUN, NfkB, HIF-1 alpha, Pax-5, Pax-8 and other transcription factors. APE/ref-1 has also been shown to control p53 activity through redox alteration. APE is linked to apoptosis, associated with thioredoxin, and altered levels of APE/ref-1 have been found in some cancers. APE appears to form a unique link between the DNA base excision pathway, oxidative signaling, transcription regulation, cancer and cell-cycle control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APE Antibody (NB100-116)(79)

Publications using NB100-116

• Antoniali G, Serra F, Lirussi L et al. Mammalian APE1 controls miRNA processing and its interactome is linked to cancer RNA metabolism. Nat Commun. 2017 Oct 06 [PMID: 28986522] (IHC-P)
• Nath S, Roychoudhury S, Kling M et al. The extracellular role of DNA damage repair protein APE1 in regulation of IL-6 expression Cell. Signal. 2017 Jul 24 [PMID: 28751279]
• Roychoudhury S, Nath S, Song H et al. Human Apurinic/Apyrimidinic Endonuclease (APE1) Is Acetylated at DNA Damage Sites in Chromatin, and Acetylation Modulates Its DNA Repair Activity. Mol Cell Biol 2017 Mar 15 [PMID: 27994014] (Human)
• Szczesny B, Marcatti M, Zatarain JR et al. Inhibition of hydrogen sulfide biosynthesis sensitizes lung adenocarcinoma to chemotherapeutic drugs by inhibiting mitochondrial DNA repair and suppressing cellular bioenergetics. Sci Rep. Nov 3 2016 [PMID: 27808278] (PLA, Human)
• Yang C, Sengupta S, Hegde PM et al. Regulation of oxidized base damage repair by chromatin assembly factor 1 subunit A. Nucleic Acids Res. Oct 27 2016 [PMID: 27794043]
• Sengupta S, Mantha AK, Song H et al. Elevated level of acetylation of APE1 in tumor cells modulates DNA damage repair. Oncotarget. Nov 15 2016 [PMID: 27655688] (WB, Human)
• Akhter N, Takeda Y, Nara H et al. Apurinic/apyrimidinic endonuclease 1/redox factor-1 (Ape1/Ref-1) modulates antigen presenting cell-mediated T helper cell type 1 responses J. Biol. Chem. Sep 16 2016 [PMID: 27637330] (WB, Mouse)
• Kelley Mr, Wikel Jh, Guo C et al. Identification of new chemical entities targeting APE1 for the prevention of chemotherapy-induced peripheral neuropathy (CIPN). J. Pharmacol. Exp. Ther. Sep 8 2016 [PMID: 27608656]
• Nikitaki Z, Nikolov V, Mavragani IV et al. Measurement of complex DNA damage induction and repair in human cellular systems after exposure to ionizing radiations of varying linear energy transfer (LET). Free Radic Res 2016 Nov [PMID: 27593437] (ICC/IF, Human)
• Lee YR, Lim JS, Shin JH et al. Altered Secretory Activity of APE1/Ref-1 D148E Variants Identified in Human Patients With Bladder Cancer. Int Neurourol J. May 1 2016 [PMID: 27230458] (WB, Human)
• Wang H, Cai S, Bailey BJ et al. Combination therapy in a xenograft model of glioblastoma: enhancement of the antitumor activity of temozolomide by an MDM2 antagonist. J. Neurosurg. May 13 2016 [PMID: 27177180]
• Moreli JB, Santos JH, Lorenzon-Ojea AR et al. Hyperglycemia Differentially Affects Maternal and Fetal DNA Integrity and DNA Damage Response Int. J. Biol. Sci. Mar 29 2016 [PMID: 27019630]
• Bhakat KK, Sengupta S, Adeniyi VF et al. Regulation of limited N-terminal proteolysis of APE1 in tumor via acetylation and its role in cell proliferation. Oncotarget 2016 Apr 19 [PMID: 26981776]
• Ahuja AK, Jodkowska K, Teloni F et al. A short G1 phase imposes constitutive replication stress and fork remodelling in mouse embryonic stem cells. Nat Commun. 2016 Feb 15 [PMID: 26876348] (WB, Mouse)
  
  Details:
  APE antibody used for WB on mouse embryonic stem cells (ESCs) that were transfected with APE1 siRNA (Supplementary Figure 6A).
• Aamann MD, Norregaard R, Kristensen ML et al. Unilateral ureteral obstruction induces DNA repair by APE1. Am. J. Physiol. Renal Physiol. 2015 Nov 25 [PMID: 26608791] (IHC-P, Rat)
• Bonora M, Wieckowsk MR, Chinopoulos C et al. Molecular mechanisms of cell death: central implication of ATP synthase in mitochondrial permeability transition. Oncogene 2015 Mar 19 [PMID: 25790189]
• Pojo M, Goncalves CS, Xavier-Magalhaes A et al. A transcriptomic signature mediated by HOXA9 promotes human glioblastoma initiation, aggressiveness and resistance to temozolomide. Oncotarget. 2015 Feb 20 [PMID: 25762636] (WB, Human)
• Yang S, Zhang J, Zhang Y et al. KDM1A triggers androgen-induced miRNA transcription via H3K4me2 demethylation and DNA oxidation Prostate 2015 Mar 01 [PMID: 25728837] (ChiP, Human)
• Fang Q, Inanc B, Schamus S et al. HSP90 regulates DnA repair via the interaction between XRCC1 and DnA polymerase B. Nat Commun. 2014 Nov 26 [PMID: 25423885] (WB)
• Woo J, Park H, Sung SH et al. Prognostic Value of Human Apurinic/Apyrimidinic Endonuclease 1 (APE1) Expression in Breast Cancer PLoS ONE. 2014 Jun 11 [PMID: 24914806] (IHC-P, Human)
  
  Details:
  APE1 antibody used for IHC-P ( pH 6.0/10 minutes microwave antigen retreival, permeablization with TBS - 0.1% Tween 20; 1:100 primary dilution, 4C ON incubation) on FFPE tissue sections of normal breast or tumors from stages I, II and III primary invasive breast cancer patients. Luminal epithelium of normal breast ducts/lobules and invasive breast cancer tissues showed nuclear APE1 expression (Figure 1 A-C).
• Kim HL, Koedrith P, Lee SM et al. Base excision DNA repair defect in thioredoxin-1 (Trx1)-deficient cells. Mutat Res 2013 Nov-Dec [PMID: 24459704] (Human)
• Xie J, Zhang L, Li M et al. Functional analysis of the involvement of apurinic/apyrimidinic endonuclease 1 in the resistance to melphalan in multiple myeloma. BMC Cancer 2014 Jan 8 [PMID: 24400589] (WB, IP, Human)
• Karmahapatra SK, Saha T, Adhikari S et al. Redox regulation of apurinic/apyrimidinic endonuclease 1 activity in Long-Evans Cinnamon rats during spontaneous hepatitis. Mol Cell Biochem 2013 Dec 15 [PMID: 24337968] (WB, Rat)
• Madlener S, Strobel T, Vose S et al. Essential role for mammalian apurinic/apyrimidinic (AP) endonuclease Ape1/Ref-1 in telomere maintenance. Proc Natl Acad Sci U S A. 2013 Oct 29 [PMID: 24127576] (IP, ChIP, Human)
• Sengupta S, Mitra S, Bhakat KK et al. Dual Regulatory Roles of Human AP-Endonuclease (APE1/Ref-1) in CDKN1A/p21 Expression. PLoS One 2013 Jul 16 [PMID: 23874636] (IP, ChIP, Human)
• Cun Y, Dai N, Xiong C et al. Silencing of APE1 Enhances Sensitivity of Human Hepatocellular Carcinoma Cells to Radiotherapy In Vitro and in a Xenograft Model. PLoS One 2013 [PMID: 23418439] (WB, Human)
• Chen S, Xiong G, Wu S, Mo J. Downregulation of Apurinic/Apyrimidinic Endonuclease 1/Redox Factor-1 Enhances the Sensitivity of Human Pancreatic Cancer Cells to Radiotherapy In Vitro. Cancer Biother Radiopharm 2012 Dec 26 [PMID: 23268706] (WB, Human)
• Yang S, Yang Z, Ji H et al. Targeting nitric oxide (NO) signaling with nNOS inhibitors as a novel strategy for the therapy and prevention of human melanoma Antioxid Redox Signal 2012 Dec 2 [PMID: 23199242] (Human)
• Linke M, May A, Reifenberg K et al. The Impact of Ovarian Stimulation on the Expression of Candidate Reprogramming Genes in Mouse Preimplantation Embryos. Cytogenet Genome Res 2012 Nov 15 [PMID: 23154201] (ICC/IF, Mouse)
• Corniola R, Zou Y, Leu D et al. Paradoxical Relationship between Mn Superoxide Dismutase Deficiency and Radiation-Induced Cognitive Defects PLoS One 2012 [PMID: 23145165] (WB, Mouse)
• Singh S, Englander EW. Nuclear depletion of apurinic/apyrimidinic endonuclease 1 (Ape1/Ref-1)is an indicator of energy disruption in neurons Free Radic Biol Med 2012 Jul 26 [PMID: 22841870] (ICC/IF, Mouse)
• Cai X, Wang J, Xin X. CIAPIN1 nuclear accumulation predicts poor clinical outcome in epithelial ovarian cancer World J Surg Oncol 2012 Jun 19 [PMID: 22713669] (IHC, Human)
• Hill JW, Poddar R, Thompson JF et al. Intranuclear matrix metalloproteinases promote DNA damage and apoptosis induced by oxygen-glucose deprivation in neurons Neuroscience 2012 Jun 15 [PMID: 22710064] (WB, Rat)
• Siddiqui A, Rivera-Sanchez S, Castro MD et al. Mitochondrial DNA damage is associated with reduced mitochondrial bioenergetics in Huntington's disease Free Radic Biol Med 2012 Jun 15 [PMID: 22709585] (ICC/IF, Mouse)
• Clark DW, Phang T, Edwards MG, Geraci MW, Gillespie MN. Promoter G-quadruplex sequences are targets for base oxidation and strand cleavage during hypoxia-induced transcription. Free Radic Biol Med. 2012 May 1. [PMID: 22583700] (ChIP, Human, Rat)
• Li M, Vascotto C, Xu S, Dai N, Qing Y, Zhong Z, Tell G, Wang D. Human AP endonuclease/redox factor APE1/ref-1modulates mitochondrial function after oxidative stress by regulating the transcriptional activity of NRF1. Free Radic Biol Med. 2012 May 11. [PMID: 22580151] (WB, Human)
• Mantha AK, Dhiman M, Taglialatela G et al. Proteomic study of amyloid beta (25-35) peptide exposure to neuronal cells: Impact on APE1/Ref-1's protein-protein interaction. J Neurosci Res;90(6):1230-9. 2012 Jun. [PMID: 22488727] (IP, Human)
• Castro MDR, Suarez E, Kraiselburd E et al. Aging increases mitochondrial DNA damage and oxidative stress in liver of rhesus monkeys. Experimental Gerontology. 2011 Oct 12. [PMID: 22027539] (WB, Primate)
• Kim YJ, Kim D, Illuzzi JL et al. S-glutathionylation of cysteine 99 in the APE1 protein impairs abasic endonuclease activity. J Mol Biol 2011 Dec 2 [PMID: 22024594] (Human)
• Weis E, Schoen H, Victor A et al. Reduced mRNA and protein expression of the genomic caretaker RAD9A in primary fibroblasts of individuals with childhood and independent second cancer. PLoS One 2011 [PMID: 21991345] (Human)
• Zhang ZM, Yang XQ, Wang D et al. Nm23-H1 protein binds to APE1 at AP sites and stimulates AP endonuclease activity following ionizing radiation of the human lung cancer A549 cells. Cell Biochem Biophys;61(3):561-72. 2011 Dec. [PMID: 21769563] (IP, WB, EMSA, Human)
• Singh-Gupta V, Joiner MC, Runyan L et al. Soy isoflavones augment radiation effect by inhibiting APE1/Ref-1 DNA repair activity in non-small cell lung cancer. J Thorac Oncol;6(4):688-98. 2011 Apr. [PMID: 21325978] (WB, Human)
• Jaiswal AS, Narayan S. Assembly of the Base Excision Repair Complex on Abasic DNA and Role of Adenomatous Polyposis Coli on Its Functional Activity. Biochemistry;50(11):1901-9. 2011 Mar 22. [PMID: 21261287] (WB, Human)
• Bobola MS, Jankowski P, Gross ME et al. Apurinic/apyrimidinic endonuclease is inversely associated with response to radiotherapy in pediatric ependymoma. Int J Cancer;129(10):2370-9. 2011 Nov 15. [PMID: 21207372] (IHC-P, Human)
• Karimi-Busheri F, Rasouli-Nia A, Mackey JR, Weinfeld M. Senescence evasion by MCF-7 human breast tumor-initiating cells. Breast Cancer Res 2010 [PMID: 20525204] (Human)
• Warters RL, Cassidy PB, Sunseri JA et al. The nuclear matrix shell proteome of human epidermis. J Dermatol Sci 2010 May [PMID: 20363599] (WB, Human)
• Jelezcova E, Trivedi RN, Wang XH et al. Parp1 activation in mouse embryonic fibroblasts promotes Pol beta-dependent cellular hypersensitivity to alkylation damage. Mutat Res 2010 Apr 1 [PMID: 20096707] (Mouse)
• Ray S, Lee C, Hou T et al. Regulation of Signal Transducer and Activator of Transcription 3 Enhanceosome Formation by Apurinic/Apyrimidinic Endonuclease 1 in Hepatic Acute Phase Response. Mol Endocrinol. 2009 Dec 23. [PMID: 20032196] (IP, ChIP, Human, Mouse)
• Jones LE Jr, Ying L, Hofseth AB et al. Differential effects of reactive nitrogen species on DNA base excision repair initiated by the alkyladenine DNA glycosylase. Carcinogenesis. 2009 Dec [PMID: 19864471] (IHC-P, Mouse)
• Batuello CN, Kelley MR, Dynlacht JR. Role of Ape1 Base Excision Repair in the Radioresponse Heat-radiosensitization of HeLa Cells. Anticancer Res;29(4):1319-1325. 2009 [PMID: 19414382] (WB, Human)
• Unnikrishnan A, Raffoul JJ, Patel HV et al. Oxidative stress alters base excision repair pathway and increases apoptotic response in apurinic/apyrimidinic endonuclease 1/redox factor-1 haploinsufficient mice. Free Radic Biol Med 2009 Jun 1 [PMID: 19268524] (WB, Mouse)
• Chattopadhyay R, Das S, Maiti AK et al. Regulatory role of human AP-endonuclease (APE1/Ref-1) in YB-1-mediated activation of the multidrug resistance gene MDR1. Mol Cell Biol;28(23):7066-80. 2008 Dec. [PMID: 18809583] (ChIP, Human)
• May A, Kirchner R, Muller H et al. Multiplex rt-PCR expression analysis of developmentally important genes in individual mouse preimplantation embryos and blastomeres. Biol Reprod;80(1):194-202. 2009 Jan. [PMID: 18784354] (ICC/IF, Mouse)
• Breit JF, Ault-Ziel K, Al-Mehdi AB et al. Nuclear protein-induced bending and flexing of the hypoxic response element of the rat vascular endothelial growth factor promoter. FASEB J 2008 Jan [PMID: 17766324] (IP, Rat)
• Yang, S et al. Redox effector factor-1, combined with reactive oxygen species, plays an important role in the transformation of JB6 cells. Carcinogenesis;28(11):2382-90. 2007 Nov. [PMID: 17566060] (WB, Human)
• Lam, W et al. The impact of hypoxic treatment on the expression of phosphoglycerate kinase and the cytotoxicity of troxacitabine and gemcitabine. Mol Pharmacol;72(3):536-44. 2007 Sep. [PMID: 17565005] (ICC/IF, Human)
• Kundu, CN et al. Adenomatous polyposis coli-mediated hypersensitivity of mouse embryonic fibroblast cell lines to methylmethane sulfonate treatment: implication of base excision repair pathways. Carcinogenesis;28(10):2089-95. 2007 Oct. [PMID: 17522063] (WB, Mouse)
• Raffoul, JJ et al. Down-regulation of Apurinic/Apyrimidinic Endonuclease 1/Redox Factor-1 Expression by Soy Isoflavones Enhances Prostate Cancer Radiotherapy In vitro In vivo. Cancer Res 67: 2141-2149. 2007 [PMID: 17332344] (WB, IHC-P, ICC/IF, Human)
• Maki A, Kono H, Gupta M et al. Predictive power of biomarkers of oxidative stress and inflammation in patients with hepatitis C virus-associated hepatocellular carcinoma. Ann Surg Oncol 2007 Mar [PMID: 17195915]
• Lam, W et al. Apurinic/apyrimidinic endonuclease-1 protein level is associated with the cytotoxicity of L-configuration deoxycytidine analogs (troxacitabine and beta-L-2',3'-dideoxy-2',3'-didehydro-5-fluorocytidine) but not D-configuration deoxycytidine analogs (gemcitabine and beta-D-arabinofuranosylcytosine). Mol Pharmacol;69(5):1607-14. 2006 May. [PMID: 16481390] (ICC/IF, Human)
• Yang, S et al. Alterations in the expression of the apurinic/apyrimidinic endonuclease-1/redox factor-1 (APE/Ref-1) in human melanoma and identification of the therapeutic potential of resveratrol as an APE/Ref-1 inhibitor. Mol Cancer Ther;4(12):1923-35. 2005 Dec. [PMID: 16373707] (ICC/IF, GS, Human)
• Bobola MS, Finn LS, Ellenbogen RG et al. Apurinic/apyrimidinic endonuclease activity is associated with response to radiation and chemotherapy in medulloblastoma and primitive neuroectodermal tumors. Clin Cancer Res;11(20):7405-14. 2005 Oct 15. [PMID: 16243814] (IHC-P, Human)
• Ziel KA, Grishko V, Campbell CC et al. Oxidants in signal transduction: impact on DNA integrity and gene expression. FASEB J 2005 Mar [PMID: 15746182] (Rat)
• Elchuri S, Oberley TD, Qi W et al. CuZnSOD deficiency leads to persistent and widespread oxidative damage and hepatocarcinogenesis later in life. Oncogene 2005 Jan 13 [PMID: 15531919]
• Cabelof, D et al. Imbalanced base excision repair in response to folate deficiency is accelerated by polymerase beta haploinsufficiency. J Biol Chem;279(35):36504-13. 2004 Aug 27. [PMID: 15218023] (WB, Mouse)
• Wang, D et al. Human apurinic endonuclease 1 (APE1) expression and prognostic significance in osteosarcoma: enhanced sensitivity of osteosarcoma to DNA damaging agents using silencing RNA APE1 expression inhibition. Mol Cancer Ther;3(6):679-86. 2004 Jun. [PMID: 15210853] (IHC, Human)
• Marenstein DR, Wilson DM 3rd, Teebor GW. Human AP endonuclease (APE1) demonstrates endonucleolytic activity against AP sites in single-stranded DNA. DNA Repair (Amst) 2004 May 4 [PMID: 15084314] (Human)
• Raffoul, J et al. Apurinic/apyrimidinic endonuclease (APE/REF-1) haploinsufficient mice display tissue-specific differences in DNA polymerase beta-dependent base excision repair. J Biol Chem;279(18):18425-33. 2004 Apr 30. [PMID: 14973123] (WB, Mouse)
• Shen, G et al. Decline of nuclear and mitochondrial oxidative base excision repair activity in late passage human diploid fibroblasts. DNA Repair (Amst);2(6):673-93. 2003 Jun 11. [PMID: 12767347] (WB, ICC/IF, Human)
• Fishel, M et al. Imbalancing the DNA base excision repair pathway in the mitochondria; targeting and overexpressing N-methylpurine DNA glycosylase in mitochondria leads to enhanced cell killing. Cancer Res;63(3):608-15. 2003 Feb 1. [PMID: 12566303] (WB, Human)
• Seo, YR et al. Implication of p53 in base excision DNA repair: in vivo evidence. Oncogene;21(5):731-7. 2002 Jan 24. [PMID: 11850801] (WB, Human, Mouse)
• Thomson, B et al. Histology-specific expression of a DNA repair protein in pediatric rhabdomyosarcomas. J Pediatr Hematol Oncol. 2001 May2001 Aug-Sep. [PMID: 11846302] (IHC, Human)
• Park, S et al. X-ray repair cross-complementing gene I protein plays an important role in camptothecin resistance. Cancer Res;62(2):459-65. 2002 Jan 15. [PMID: 11809696] (ICC/IF, WB, Human)
• Schindl, M et al. DNA repair-redox enzyme apurinic endonuclease in cervical cancer: evaluation of redox control of HIF-1alpha and prognostic significance. Int J Oncol;19(4):799-802. 2001 Oct. [PMID: 11562758] (IHC, Human)
• Kelley, Mark R et al. Elevated and altered expression of the multifunctional DNA base excision repair and redox enzyme Ape1/ref-1 in prostate cancer. Clin Cancer Res;7(4):824-30. 2001 Apr. [PMID: 11309329] (WB, IHC, Human)
• Robertson, Kent A et al. Altered expression of Ape1/ref-1 in germ cell tumors and overexpression in NT2 cells confers resistance to bleomycin and radiation. Cancer Res;61(5):2220-5. 2001 Mar 1. [PMID: 11280790] (IHC-P, Human)
• Thomson, B et al. Apurinic/apyrimidinic endonuclease expression in pediatric yolk sac tumors. Anticancer Res;20(6B):4153-7. 2000 Nov-Dec. [PMID: 11205241] (IHC, Human)
• Chou, K et al. A novel action of human apurinic/apyrimidinic endonuclease: excision of L-configuration deoxyribonucleoside analogs from the 3' termini of DNA. J Biol Chem;275(40):31009-15. 2000 Oct 6. [PMID: 10906132] (WB, Human)
• Moore, David H et al. Alterations in the expression of the DNA repair/redox enzyme APE/ref-1 in epithelial ovarian cancers. Clin Cancer Res;6(2):602-9. 2000 Feb. [PMID: 10690545] (WB, IHC-P, Human)

Reviews for APE Antibody (NB100-116) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Lisa Lirussi WB Human 03/28/2014

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human

Blocking

• Blocking Details: 5% milk in PBS-T for 1hr at RT

Primary Anitbody

• Dilution Ratio: 1:2000 for 2hr at RT in blocking solution

Secondary Antibody

• Secondary Description: Goat anti-mouse IgG
• Secondary Concentration: 1:10000

Details

• Detection Notes: Fluorescence

reviewed by: Anonymous WB Human 09/08/2009

Summary

• Application: Western Blot
• Sample Tested: Human melanoma cells, Sample Amount: 25ug
• Species: Human
• Lot: B7

Blocking

• Blocking Details: Blocking Buffer: 5% non-fat milk, Blocking Time: 2 hours, Blocking Temp: Room temperature

Primary Anitbody

• Dilution Ratio: Dilution Ratio: 1/3000, Incubation Dilution Buffer: 5% non-fat milk, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

• Secondary Description: Secondary Ab: Santa Cruz, Secondary Ab Dilution Ratio: 1/1000

Details

• Detection Notes: Detection Method: substrate hrp, Exposure Time: 5 seconds, Specific Bands: 39 kDa

FAQs for APE Antibody (NB100-116). (Showing 1 - 2 of 2 FAQs).

1. I have problems performing IF on HeLa cells with this antibody (NB100-116). I have a very weird pattern of staining. Is there a special protocol I need to follow?
   • Please see the following link for our product specific protocol for NB100-116.
2. Today I have a customer who is having some difficulty with NB100-116 in his IP experiments. I have sent him a link to a journal that references use of the antibody in IP, but he has now asked if the antibody recognizes the reduced form of APE1 and not the oxidized form. Do you have any information available?
   • It seems that in Western blot, Novus as well as our customers use reducing conditions, as is standard. I have also looked at our publications using this antibody in IP and nothing suggests that it needs to be used in reducing conditions for IP. It would seem that it recognizes both forms and should work in IP in most standard IP protocols.

Bioinformatics

• Gene Symbol: APEX1
• Entrez:
  • 328(Human)
  • 11792(Mouse)
  • 305605(Rat)
  • 79116(Rat)
• Uniprot:
  • P27695()