APE Antibody (13B8E5C2) (NB100-116)

Images

 
Western Blot: APE Antibody (13B8E5C2) [NB100-116] - Ovarian Cancer cell lines.
Immunocytochemistry/ Immunofluorescence: APE Antibody (13B8E5C2) [NB100-116] - HeLa cells stained NB100-116 (Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are ...read more
Immunohistochemistry-Paraffin: APE Antibody (13B8E5C2) [NB100-116] - APE1 antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: APE Antibody (13B8E5C2) [NB100-116] - Immunocytochemical detection of APE-ref-1 in breast cancer cell line MDS231.
Simple Western: APE Antibody (13B8E5C2) [NB100-116] - Simple Western lane view shows a specific band for APE1 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ChIP, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA
Clone
13B8E5C2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

APE Antibody (13B8E5C2) Summary

Immunogen
Purified human APE1 [UniProt# P27695]
Epitope
The epitope is within amino acids 80-100.
Localization
In immunohistochemical experiments punctate nuclear staining is observed in most cell types. Cytoplasmic staining may be seen in hepatocytes, large neurons and granule cells.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
APEX1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Simple Western 1:25
  • Chromatin Immunoprecipitation 1:10-1:500
  • Gel Super Shift Assays
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
  • Proximity Ligation Assay
Application Notes
This APE1 (13B8E5C2) antibody is useful for Western Blot, Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Gel Super Shift Assays was reported in scientific literature. In WB this antibody detects a single band at 37 kDa. In IHC, it can be competitively inhibited from recognizing the APE1 antigen in tissues using APE1 protein. It can also be used on frozen and fixed-paraffin sections and cytospin preps. In IHC-P, staining was observed in the nucleus of a human breast cancer xenograft. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Proximity Ligation Assay reported in scientific literature (PMID 27808278).
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Antagonist
Positive Control
HeLa Lysate (NB800-PC1)
APE Lysate (NBL1-07597)
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-116 in the following applications:

Publications
Read Publications using
NB100-116 in the following applications:

  • 5 publications
  • 1 publication
  • 1 publication
  • GS
    1 publication
  • 11 publications
  • IHC
    6 publications
  • 8 publications
  • IP
    7 publications
  • PLA
    1 publication
  • WB
    32 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for APE Antibody (13B8E5C2)

  • AP endonuclease 1
  • APE
  • APE1
  • APE-1
  • APEAPEX nuclease (multifunctional DNA repair enzyme)
  • APENAP endonuclease class I
  • APEX deoxyribonuclease (apurinic or apyrimidinic)
  • APEX nuclease (multifunctional DNA repair enzyme) 1
  • APEX nuclease
  • APEX1
  • Apurinic-apyrimidinic endonuclease 1
  • APXAP lyase
  • DNA-(apurinic or apyrimidinic site) lyase
  • EC 3.1
  • EC 4.2.99.18
  • HAP1apurinic/apyrimidinic (abasic) endonuclease
  • multifunctional DNA repair enzyme
  • protein REF-1
  • redox factor 1
  • Redox factor-1
  • REF1 apurinic/apyrimidinic exonuclease
  • REF-1

Background

The mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is responsible for the repair of AP sites in DNA. In addition, this enzyme functions as a redox factor facilitating the DNA binding capability of FOS, JUN, NfkB, HIF-1 alpha, Pax-5, Pax-8 and other transcription factors. APE/ref-1 has also been shown to control p53 activity through redox alteration. APE is linked to apoptosis, associated with thioredoxin, and altered levels of APE/ref-1 have been found in some cancers. APE appears to form a unique link between the DNA base excision pathway, oxidative signaling, transcription regulation, cancer and cell-cycle control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-116
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ChIP, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA

Publications for APE Antibody (NB100-116)(78)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate.

We have publications tested in 10 applications: ChIP, ChiP, EMSA, GS, ICC/IF, IHC, IHC-P, IP, PLA, WB.


Filter By Application
ChIP
(5)
ChiP
(1)
EMSA
(1)
GS
(1)
ICC/IF
(11)
IHC
(6)
IHC-P
(8)
IP
(7)
PLA
(1)
WB
(32)
All Applications
Filter By Species
Human
(49)
Mouse
(15)
Rat
(6)
Primate
(1)
All Species
Showing Publications 1 - 10 of 78. Show All 78 Publications.
Publications using NB100-116 Applications Species
Nath S, Roychoudhury S, Kling M et al. The extracellular role of DNA damage repair protein APE1 in regulation of IL-6 expression Cell. Signal. 2017 Jul 24 [PMID: 28751279]
Roychoudhury S, Nath S, Song H et al. Human Apurinic/Apyrimidinic Endonuclease (APE1) Is Acetylated at DNA Damage Sites in Chromatin, and Acetylation Modulates Its DNA Repair Activity. Mol Cell Biol 2017 Mar 15 [PMID: 27994014] (Human) Human
Szczesny B, Marcatti M, Zatarain JR et al. Inhibition of hydrogen sulfide biosynthesis sensitizes lung adenocarcinoma to chemotherapeutic drugs by inhibiting mitochondrial DNA repair and suppressing cellular bioenergetics. Sci Rep. Nov 3 2016 [PMID: 27808278] (PLA, Human) PLA Human
Yang C, Sengupta S, Hegde PM et al. Regulation of oxidized base damage repair by chromatin assembly factor 1 subunit A. Nucleic Acids Res. Oct 27 2016 [PMID: 27794043]
Sengupta S, Mantha AK, Song H et al. Elevated level of acetylation of APE1 in tumor cells modulates DNA damage repair. Oncotarget. Nov 15 2016 [PMID: 27655688] (WB, Human) WB Human
Akhter N, Takeda Y, Nara H et al. Apurinic/apyrimidinic endonuclease 1/redox factor-1 (Ape1/Ref-1) modulates antigen presenting cell-mediated T helper cell type 1 responses J. Biol. Chem. Sep 16 2016 [PMID: 27637330] (WB, Mouse) WB Mouse
Kelley Mr, Wikel Jh, Guo C et al. Identification of new chemical entities targeting APE1 for the prevention of chemotherapy-induced peripheral neuropathy (CIPN). J. Pharmacol. Exp. Ther. Sep 8 2016 [PMID: 27608656]
Nikitaki Z, Nikolov V, Mavragani IV et al. Measurement of complex DNA damage induction and repair in human cellular systems after exposure to ionizing radiations of varying linear energy transfer (LET). Free Radic Res 2016 Nov [PMID: 27593437] (ICC/IF, Human) ICC/IF Human
Lee YR, Lim JS, Shin JH et al. Altered Secretory Activity of APE1/Ref-1 D148E Variants Identified in Human Patients With Bladder Cancer. Int Neurourol J. May 1 2016 [PMID: 27230458] (WB, Human) WB Human
Wang H, Cai S, Bailey BJ et al. Combination therapy in a xenograft model of glioblastoma: enhancement of the antitumor activity of temozolomide by an MDM2 antagonist. J. Neurosurg. May 13 2016 [PMID: 27177180]
Show All 78 Publications.

Reviews for APE Antibody (NB100-116) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 review tested in 1 application: WB.

Reviews using NB100-116:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Lisa Lirussi
WB Human 03/28/2014
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman

Blocking

Blocking Details5% milk in PBS-T for 1hr at RT

Primary Anitbody

Dilution Ratio1:2000 for 2hr at RT in blocking solution

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG
Secondary Concentration1:10000

Details

Detection NotesFluorescence
 
reviewed by:
Anonymous
WB Human 09/08/2009
View

Summary

ApplicationWestern Blot
Sample TestedHuman melanoma cells, Sample Amount: 25ug
SpeciesHuman
LotB7

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat milk, Blocking Time: 2 hours, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1/3000, Incubation Dilution Buffer: 5% non-fat milk, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: Santa Cruz, Secondary Ab Dilution Ratio: 1/1000

Details

Detection NotesDetection Method: substrate hrp, Exposure Time: 5 seconds, Specific Bands: 39 kDa

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for APE Antibody (NB100-116). (Showing 1 - 2 of 2 FAQs).

  1. I have problems performing IF on HeLa cells with this antibody (NB100-116). I have a very weird pattern of staining. Is there a special protocol I need to follow?
    • Please see the following link for our product specific protocol for NB100-116.
  2. Today I have a customer who is having some difficulty with NB100-116 in his IP experiments. I have sent him a link to a journal that references use of the antibody in IP, but he has now asked if the antibody recognizes the reduced form of APE1 and not the oxidized form. Do you have any information available?
    • It seems that in Western blot, Novus as well as our customers use reducing conditions, as is standard. I have also looked at our publications using this antibody in IP and nothing suggests that it needs to be used in reducing conditions for IP. It would seem that it recognizes both forms and should work in IP in most standard IP protocols.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

 
Lisa Lirussi
03/28/2014
Application: WB
Species: Human

 
Anonymous
09/08/2009
Application: WB
Species: Human

Bioinformatics

Gene Symbol APEX1
Entrez
Uniprot