APE Antibody (13B8E5C2)

Images

 
Western Blot: APE Antibody (13B8E5C2) [NB100-116] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% ...read more
Immunocytochemistry/ Immunofluorescence: APE Antibody (13B8E5C2) [NB100-116] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells ...read more
Immunohistochemistry-Paraffin: APE Antibody (13B8E5C2) [NB100-116] - APE1 antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain.
Western Blot: APE Antibody (13B8E5C2) [NB100-116] - Ovarian Cancer cell lines.
Immunocytochemistry/ Immunofluorescence: APE Antibody (13B8E5C2) [NB100-116] - Immunocytochemical detection of APE-ref-1 in breast cancer cell line MDA MB 231.
Immunocytochemistry/ Immunofluorescence: APE Antibody (13B8E5C2) [NB100-116] - HeLa cells stained NB100-116 (Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are ...read more
Simple Western: APE Antibody (13B8E5C2) [NB100-116] - Image shows a specific band for APE1 in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ChIP, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA
Clone
13B8E5C2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

APE Antibody (13B8E5C2) Summary

Immunogen
Purified human APE1 [Uniprot: P27695]
Epitope
The epitope is within amino acids 80-100.
Localization
In immunohistochemical experiments punctate nuclear staining is observed in most cell types. Cytoplasmic staining may be seen in hepatocytes, large neurons and granule cells.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
APEX1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Simple Western 1:25
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Gel Super Shift Assays
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10 - 1:500
  • Proximity Ligation Assay
Application Notes
Gel Super Shift Assays was reported in scientific literature. In Western blot, this antibody detects a single band at 37 kDa. In IHC, it can be competitively inhibited from recognizing the APE1 antigen in tissues using APE1 protein. It can also be used on frozen and fixed-paraffin sections and cytospin preps. In IHC-P, staining was observed in the nucleus of a human breast cancer xenograft. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in Proximity Ligation Assay reported in scientific literature (PMID: 27808278). Use in immunoblotting reported in scientific literature (PMID: 27608656).
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Antagonist
Control
HeLa Whole Cell Lysate
APE Overexpression Lysate
APE Knockout 293T Cell Lysate
Reviewed Applications
Read 3 Reviews rated 5
using
NB100-116 in the following applications:

Publications
Read Publications using
NB100-116 in the following applications:

  • 7 publications
  • 1 publication
  • 1 publication
  • GS
    1 publication
  • 11 publications
  • IHC
    8 publications
  • 9 publications
  • IP
    8 publications
  • PLA
    1 publication
  • WB
    37 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for APE Antibody (13B8E5C2)

  • AP endonuclease 1
  • APE
  • APE1
  • APE-1
  • APEAPEX nuclease (multifunctional DNA repair enzyme)
  • APENAP endonuclease class I
  • APEX deoxyribonuclease (apurinic or apyrimidinic)
  • APEX nuclease (multifunctional DNA repair enzyme) 1
  • APEX nuclease
  • APEX1
  • Apurinic-apyrimidinic endonuclease 1
  • APXAP lyase
  • DNA-(apurinic or apyrimidinic site) lyase
  • EC 3.1
  • EC 4.2.99.18
  • HAP1apurinic/apyrimidinic (abasic) endonuclease
  • multifunctional DNA repair enzyme
  • protein REF-1
  • redox factor 1
  • Redox factor-1
  • REF1 apurinic/apyrimidinic exonuclease
  • REF-1

Background

The mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is responsible for the repair of AP sites in DNA. In addition, this enzyme functions as a redox factor facilitating the DNA binding capability of FOS, JUN, NfkB, HIF-1 alpha, Pax-5, Pax-8 and other transcription factors. APE/ref-1 has also been shown to control p53 activity through redox alteration. APE is linked to apoptosis, associated with thioredoxin, and altered levels of APE/ref-1 have been found in some cancers. APE appears to form a unique link between the DNA base excision pathway, oxidative signaling, transcription regulation, cancer and cell-cycle control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-116
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ChIP, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA

Publications for APE Antibody (NB100-116)(88)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate.

We have publications tested in 10 applications: ChIP, ChiP, EMSA, GS, ICC/IF, IHC, IHC-P, IP, PLA, WB.


Filter By Application
ChIP
(7)
ChiP
(1)
EMSA
(1)
GS
(1)
ICC/IF
(11)
IHC
(8)
IHC-P
(9)
IP
(8)
PLA
(1)
WB
(37)
All Applications
Filter By Species
Human
(55)
Mouse
(17)
Rat
(6)
Primate
(1)
All Species
Showing Publications 1 - 10 of 88. Show All 88 Publications.
Publications using NB100-116 Applications Species
Sun Y, Feng Y, Zhang G, Xu Y The endonuclease APE1 processes miR-92b formation, thereby regulating expression of the tumor suppressor LDLR in cervical cancer cells Ther Adv Med Oncol Jul 10 2019 [PMID: 31320936] (IHC, Human) IHC Human
Malfatti MC, Gerratana L, Dalla E et al. APE1 and NPM1 protect cancer cells from platinum compounds cytotoxicity and their expression pattern has a prognostic value in TNBC J. Exp. Clin. Cancer Res. Jul 15 2019 [PMID: 31307523] (WB, IHC, Human) WB, IHC Human
Lee YR, Joo HK, Lee EO et al. ATP Binding Cassette Transporter A1 is Involved in Extracellular Secretion of Acetylated APE1/Ref-1 Int J Mol Sci Jun 28 2019 [PMID: 31261750] (IP, Human) IP Human
George JW, Bessho M, Bessho T. Inactivation of XPF Sensitizes Cancer Cells to Gemcitabine J Nucleic Acids Mar 3 2019 [PMID: 30941207] (WB, Human) WB Human
Zhang, Q;Chao, TC;Patil, VS;Qin, Y;Tiwari, SK;Chiou, J;Dobin, A;Tsai, CM;Li, Z;Dang, J;Gupta, S;Urdahl, K;Nizet, V;Gingeras, TR;Gaulton, KJ;Rana, TM; The long noncoding RNA ROCKI regulates inflammatory gene expression EMBO J. Apr 15 2019 [PMID: 30918008] (ChIP, Human) ChIP Human
Cho W, Jin X, Pang J et al. The Molecular Chaperone HSP70 Controls Liver Cancer Initiation and Progression by Regulating Adaptive DNA-Damage and MAPK/ERK Signaling Pathways Mol. Cell. Biol. Feb 11 2019 [PMID: 30745413] (WB) WB
Frossi B, Antoniali G, Yu K et al. Endonuclease and redox activities of human apurinic/apyrimidinic endonuclease 1 have distinctive and essential functions in IgA class switch recombination J. Biol. Chem. Jan 31 2019 [PMID: 30705092] (WB, Mouse) WB Mouse
Antoniali G, Serra F, Lirussi L et al. Mammalian APE1 controls miRNA processing and its interactome is linked to cancer RNA metabolism. Nat Commun. 2017 Oct 06 [PMID: 28986522] (IHC-P) IHC-P
Nath S, Roychoudhury S, Kling M et al. The extracellular role of DNA damage repair protein APE1 in regulation of IL-6 expression Cell. Signal. 2017 Jul 24 [PMID: 28751279]
Roychoudhury S, Nath S, Song H et al. Human Apurinic/Apyrimidinic Endonuclease (APE1) Is Acetylated at DNA Damage Sites in Chromatin, and Acetylation Modulates Its DNA Repair Activity. Mol Cell Biol 2017 Mar 15 [PMID: 27994014] (Human) Human
Show All 88 Publications.

Reviews for APE Antibody (NB100-116) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-116:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Luiz Alexandre Magno
WB Mouse 07/15/2018
View

Summary

ApplicationWestern Blot
Sample TestedBlood mononuclear cells (PBMCs)
SpeciesMouse
LotC-3
 
reviewed by:
Lisa Lirussi
WB Human 03/28/2014
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman

Blocking

Blocking Details5% milk in PBS-T for 1hr at RT

Primary Anitbody

Dilution Ratio1:2000 for 2hr at RT in blocking solution

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG
Secondary Concentration1:10000

Details

Detection NotesFluorescence
 
reviewed by:
Anonymous
WB Human 09/08/2009
View

Summary

ApplicationWestern Blot
Sample TestedHuman melanoma cells, Sample Amount: 25ug
SpeciesHuman
LotB7

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat milk, Blocking Time: 2 hours, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1/3000, Incubation Dilution Buffer: 5% non-fat milk, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: Santa Cruz, Secondary Ab Dilution Ratio: 1/1000

Details

Detection NotesDetection Method: substrate hrp, Exposure Time: 5 seconds, Specific Bands: 39 kDa

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for APE Antibody (NB100-116). (Showing 1 - 2 of 2 FAQs).

  1. I have problems performing IF on HeLa cells with this antibody (NB100-116). I have a very weird pattern of staining. Is there a special protocol I need to follow?
    • Please see the following link for our product specific protocol for NB100-116.
  2. Today I have a customer who is having some difficulty with NB100-116 in his IP experiments. I have sent him a link to a journal that references use of the antibody in IP, but he has now asked if the antibody recognizes the reduced form of APE1 and not the oxidized form. Do you have any information available?
    • It seems that in Western blot, Novus as well as our customers use reducing conditions, as is standard. I have also looked at our publications using this antibody in IP and nothing suggests that it needs to be used in reducing conditions for IP. It would seem that it recognizes both forms and should work in IP in most standard IP protocols.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-116AF350
Alexa Fluor 405 NB100-116AF405
Alexa Fluor 488 NB100-116AF488
Alexa Fluor 532 NB100-116AF532
Alexa Fluor 594 NB100-116AF594
Alexa Fluor 647 NB100-116AF647
Alexa Fluor 700 NB100-116AF700
Alexa Fluor 750 NB100-116AF750
Biotin NB100-116B
DyLight 350 NB100-116UV
DyLight 405 NB100-116V
DyLight 488 NB100-116G
DyLight 550 NB100-116R
DyLight 594 NB100-116DL594
DyLight 650 NB100-116C
DyLight 680 NB100-116FR
DyLight 755 NB100-116IR
FITC NB100-116F
HRP NB100-116H
Janelia Fluor 549 NB100-116JF549
Janelia Fluor 646 NB100-116JF646

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Bioinformatics Tool for APE Antibody (NB100-116)

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Blogs on APE

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

 
Luiz Alexandre Magno
07/15/2018
Application: WB
Species: Mouse

 
Lisa Lirussi
03/28/2014
Application: WB
Species: Human

 
Anonymous
09/08/2009
Application: WB
Species: Human

Bioinformatics

Gene Symbol APEX1
Entrez
Uniprot