NONO Antibody

Images

 
Western Blot: NONO Antibody [NB100-1556] - B-cell whole cell lysate 40ug. This image was submitted via customer review.
Immunocytochemistry: NONO Antibody [NB100-1556] - NONO in human fibroblasts at 1:200 dilution in 1X PBS/1% BSA/0.3% Triton X-100, 16 hours 4C. This image was submitted by customer review.
Immunohistochemistry-Paraffin: NONO Antibody [NB100-1556] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- NONO used at a ...read more
Independent Antibodies: Western Blot: NONO Antibody [NB100-1556] - Detection of Human NONO on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T cell lystate (50 microgram) using NB100-1556. NONO was also ...read more
Biological Strategies: Western Blot: NONO Antibody [NB100-1556] - Whole cell lysate NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells.
Proximity Ligation Assay: NONO Antibody [NB100-1556] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human NONO (NB100-1556). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: NONO Antibody [NB100-1556] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMARCA4 (NBP2-22234) and rabbit anti-human NONO (NB100-1556). Image merged from DAPI (2ms) and ...read more
Proximity Ligation Assay: NONO Antibody [NB100-1556] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human FUS (NBP2-14860) and rabbit anti-human NONO (NB100-1556). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: NONO Antibody [NB100-1556] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human POLR2A (NBP2-14859) and rabbit anti-human NONO (NB100-1556). Image merged from DAPI (2ms), ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, IP, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

NONO Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 350 and 400 of human Non-POU Domain Containing, Octamer-binding using the numbering given in entry NP_031389.3 (GeneID 4841).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NONO
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:200 - 1:1:000
  • Immunohistochemistry-Paraffin 1:200 - 1:1:000
  • Immunoprecipitation 1-4 ug/mg
  • Immunocytochemistry
Application Notes
ICC reactivity per customer review.
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
NONO Lysate (NBL1-13717)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-1556 in the following applications:

Publications
Read Publications using NB100-1556.

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Orangutan.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NONO Antibody

  • NMT5552 kDa subunit
  • non-POU domain containing, octamer-binding
  • NRB54non-POU-domain-containing, octamer-binding
  • p54(nrb)

Background

Non-POU-domain-containing octamer binding protein (NONO) is a member of the DBHS (drosophila behavior, human splicing) domain-containing family and is an RNA- and DNA- binding protein. NONO and other DBHS domain-containing proteins are multifunctional and are reported to be involved in transcriptional regulation, mRNA processing, and DNA non-homologous end joining (NHEJ). NONO functions as a coregulator of the androgen receptor (AR) and also regulates cAMP transcriptional activity by interacting with gene promoter elements. NONO is also involved in pre-mRNA splicing through an interaction with U5 snRNA and can stimulate DNA nonhomologous end joining (NHEJ) through the interaction with ku70/G22p and ku80/XRCC5 dimers. Alternate names for NONO include 54 kDa nuclear RNA- and DNA-binding protein, p54 (nrb), 55 kDa nuclear protein, NMT55, DNA-binding p52/p100 complex, NRB54, P54, and P54NRB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-1556
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Applications: WB, IHC, IHC-P, IP, ICC/IF

Publications for NONO Antibody (NB100-1556)(3)

Reviews for NONO Antibody (NB100-1556) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB100-1556:
Filter by Applications
WB
(1)
ICC
(1)
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Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
Western Blot NONO NB100-1556
Enlarge
reviewed by:
Anonymous
WB Human 03/09/2018
View

Summary

ApplicationWestern Blot
Sample TestedB-cell whole cell lysate 40ug
SpeciesHuman
LotA1

Comments

CommentsBIOSCARF
Immunocytochemistry NONO NB100-1556
Enlarge
reviewed by:
Anonymous
ICC Human 01/11/2018
View

Summary

ApplicationImmunocytochemistry
Sample Tested fibroblasts
SpeciesHuman
LotA1

Comments

Comments1:200 in 1X PBS/1% BSA/0.3% Triton X-100, 16 hours 4C.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NONO Antibody (NB100-1556) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for NONO Antibody (NB100-1556)

Discover related pathways, diseases and genes to NONO Antibody (NB100-1556). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for NONO Antibody (NB100-1556)

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Pathways for NONO Antibody (NB100-1556)

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PTMs for NONO Antibody (NB100-1556)

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Research Areas for NONO Antibody (NB100-1556)

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Blogs on NONO

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Anonymous
03/09/2018
Application: WB
Species: Human

Anonymous
01/11/2018
Application: ICC
Species: Human

Bioinformatics

Gene Symbol NONO
Entrez
Uniprot