Western Blot: NONO Antibody [NB100-1556] - B-cell whole cell lysate 40ug. Image submitted by a verified customer review
Immunocytochemistry: NONO Antibody [NB100-1556] - NONO in human fibroblasts at 1:200 dilution in 1X PBS/1% BSA/0.3% Triton X-100, 16 hours 4C. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: NONO Antibody [NB100-1556] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- NONO used at a ...read more
Independent Antibodies: Western Blot: NONO Antibody [NB100-1556] - Detection of Human NONO on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T cell lystate (50 microgram) using NB100-1556. NONO was also ...read more
Biological Strategies: Western Blot: NONO Antibody [NB100-1556] - Whole cell lysate NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells.
The immunogen recognized by this antibody maps to a region between residues 350 and 400 of human Non-POU Domain Containing, Octamer-binding using the numbering given in entry NP_031389.3 (GeneID 4841).
Primate (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
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NONO antibody validated for ICC/IF, WB from verified customer reviews. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
54 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Based on 100% sequence identity, this antibody is predicted to react with Orangutan.
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
TBS and 0.1% BSA
0.09% Sodium Azide
Immunogen affinity purified
Alternate Names for NONO Antibody
NMT5552 kDa subunit
non-POU domain containing, octamer-binding
Non-POU-domain-containing octamer binding protein (NONO) is a member of the DBHS (drosophila behavior, human splicing) domain-containing family and is an RNA- and DNA- binding protein. NONO and other DBHS domain-containing proteins are multifunctional and are reported to be involved in transcriptional regulation, mRNA processing, and DNA non-homologous end joining (NHEJ). NONO functions as a coregulator of the androgen receptor (AR) and also regulates cAMP transcriptional activity by interacting with gene promoter elements. NONO is also involved in pre-mRNA splicing through an interaction with U5 snRNA and can stimulate DNA nonhomologous end joining (NHEJ) through the interaction with ku70/G22p and ku80/XRCC5 dimers. Alternate names for NONO include 54 kDa nuclear RNA- and DNA-binding protein, p54 (nrb), 55 kDa nuclear protein, NMT55, DNA-binding p52/p100 complex, NRB54, P54, and P54NRB.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.