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NONO Antibody

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Western Blot: NONO Antibody [NB100-1556] - B-cell whole cell lysate 40ug. This image was submitted via customer review.
Western Blot: NONO Antibody [NB100-1556] - B-cell whole cell lysate 40ug. This image was submitted via customer review.
Immunocytochemistry: NONO Antibody [NB100-1556] - NONO in human fibroblasts at 1:200 dilution in 1X PBS/1% BSA/0.3% Triton X-100, 16 hours 4C. This image was submitted by customer review.
Immunohistochemistry-Paraffin: NONO Antibody [NB100-1556] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- NONO used at a ...read more
Independent Antibodies: Western Blot: NONO Antibody [NB100-1556] - Detection of Human NONO on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T cell lystate (50 microgram) using NB100-1556. NONO was also ...read more
Biological Strategies: Western Blot: NONO Antibody [NB100-1556] - Whole cell lysate NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, IP, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

  

Biological Strategies

      

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NONO Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 350 and 400 of human Non-POU Domain Containing, Octamer-binding using the numbering given in entry NP_031389.3 (GeneID 4841).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NONO
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:200 - 1:1:000
  • Immunohistochemistry-Paraffin 1:200 - 1:1:000
  • Immunoprecipitation 1-4 ug/mg
  • Immunocytochemistry
Application Notes
ICC reactivity per customer review.
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
NONO Lysate (NBL1-13717)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-1556 in the following applications:
Publications
Read Publications using NB100-1556.

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Orangutan.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NONO Antibody

  • NMT5552 kDa subunit
  • non-POU domain containing, octamer-binding
  • NRB54non-POU-domain-containing, octamer-binding
  • p54(nrb)

Background

Non-POU-domain-containing octamer binding protein (NONO) is a member of the DBHS (drosophila behavior, human splicing) domain-containing family and is an RNA- and DNA- binding protein. NONO and other DBHS domain-containing proteins are multifunctional and are reported to be involved in transcriptional regulation, mRNA processing, and DNA non-homologous end joining (NHEJ). NONO functions as a coregulator of the androgen receptor (AR) and also regulates cAMP transcriptional activity by interacting with gene promoter elements. NONO is also involved in pre-mRNA splicing through an interaction with U5 snRNA and can stimulate DNA nonhomologous end joining (NHEJ) through the interaction with ku70/G22p and ku80/XRCC5 dimers. Alternate names for NONO include 54 kDa nuclear RNA- and DNA-binding protein, p54 (nrb), 55 kDa nuclear protein, NMT55, DNA-binding p52/p100 complex, NRB54, P54, and P54NRB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: SFPQ Antibody [NBP2-20329] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SFPQ antibody diluted by 1:10000.
Immunocytochemistry/Immunofluorescence: SFPQ Antibody [NBP2-20329] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SFPQ protein stained by SFPQ antibody [N2C1], Internal diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.
Immunohistochemistry-Paraffin: SFPQ Antibody [NBP2-20329] - Paraffin-embedded mouse fore brain. SFPQ antibody [N2C1], Internal diluted at 1:500.
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Western Blot: APH1A Antibody [NB100-74360] - Analysis of mouse brain and kidney extracts.
Immunocytochemistry/Immunofluorescence: APH1A Antibody [NB100-74360] - Staining of APH-1 in human KNS-42 glioma cells.
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Western blot shows lysates of CHP-100 human neuroblastoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
JNK1 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Western Blot: DDX6 Antibody [NB200-191] - Detection of human DDX6 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX6 antibody NB200-191 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP. DDX6 was also immunoprecipitated using rabbit anti-DDX6 antibody NB200-192, but not with 'DDX6 Ab', which is to another epitope from DDX6. Normal rabbit IgG was used as a negative control. Detection: Chemiluminescence with an exposure time of 1 second (A & B).
Immunohistochemistry: DDX6 Antibody [NB200-191] - Sample: FFPE section of human colon carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDX6 used at a dilution of 1:200 (1ug/ml). Detection: DAB.
Immunofluorescence: DDX6 Antibody [NB200-191] - In normal condition, cells were fixed and stained for DDX6, that usually punctuate in process bodies (PBs). Primary Antibody rabbit-anti-DDX6 diluted 1:1000. This image was submitted via customer Review.
(1 Review)
(29 Publications)
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Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Western blot analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Immunofluorescence analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: c-jun Antibody (5B1) [NBP2-37576] - Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using c-Jun mouse mAb with DAB staining.
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Immunocytochemistry/Immunofluorescence: PSPC1 Antibody [NBP1-83801] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining shown in green.
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Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.
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Western Blot: SKAR Antibody [NBP1-92392] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
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Immunohistochemistry-Paraffin: SKAR Antibody [NBP1-92392] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
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Immunocytochemistry/Immunofluorescence: PSMC6 Antibody (2C4) [H00005706-M02] - Analysis of monoclonal antibody to PSMC6 on HeLa cell. Antibody concentration 10 ug/ml.
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Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for p38 alpha at approximately 43 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
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COLO 205 human colorectal adenocarcinoma cell line was stained with Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled histogram) or isotype control antibody (Catalog #AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.
D3 mouse embryonic stem cell line was stained with Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled histogram) or isotype control antibody (Catalog #AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.
EphB2 was detected in immersion fixed paraffin-embedded sections of human esophageal squamous cell carcinoma using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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SF-1/NR5A1/Steroidogen ...
PP-N1665-00
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(2 Reviews)
(1 Publication)
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Western Blot: POLDIP2 Antibody [NBP1-68874] - (1ug/ml) Analysis of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: POLDIP2 Antibody [NBP1-68874] - (0.5ug/ml) Analysis of Rat VSMC lysate (35ug protein in RIPA buffer). Primary incubation was overnight at 4C. Detected by chemiluminescence. Data provided Dr. B. Lassegue, Emory University, Atlanta, USA
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, PEP-ELISA
Crk Antibody
(3G11E8)
NBP2-37568
Western Blot: Crk Antibody (3G11E8) [NBP2-37568] - Western blot analysis using CRK mAb against HEK293 (1) and CRK(AA: 1-204)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: Crk Antibody (3G11E8) [NBP2-37568] - Immunofluorescence analysis of 3T3-L1 cells using CRK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: Crk Antibody (3G11E8) [NBP2-37568] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using CRK mouse mAb with DAB staining.
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC
GRAP2 Antibody
(OTI1G2)
NBP2-00764
Western Blot: GRAP2 Antibody (1G2) [NBP2-00764] Analysis of extracts (35ug) from 9 different cell lines by using anti-GRAP2 monoclonal antibody.
Immunohistochemistry-Paraffin: GRAP2 Antibody (1G2) [NBP2-00764] - Staining of paraffin-embedded Human endometrium tissue using anti-GRAP2 mouse monoclonal antibody.
Flow Cytometry: GRAP2 Antibody (1G2) [NBP2-00764] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GRAP2 antibody, and then analyzed by flow cytometry.
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NONO Antibody
NB100-1556
NONO Antibody
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP, ICC/IF

Publications for NONO Antibody (NB100-1556)(3)


Reviews for NONO Antibody (NB100-1556) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB100-1556:
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WB
(1)
ICC
(1)
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(2)
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Images Ratings Applications Species Date Details
Western Blot NONO NB100-1556
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reviewed by:
Anonymous
 WB Human 03/09/2018
View

Summary

ApplicationWestern Blot
Sample TestedB-cell whole cell lysate 40ug
SpeciesHuman
LotA1

Comments

CommentsBIOSCARF
Immunocytochemistry NONO NB100-1556
Enlarge
reviewed by:
Anonymous
 ICC Human 01/11/2018
View

Summary

ApplicationImmunocytochemistry
Sample Tested fibroblasts
SpeciesHuman
LotA1

Comments

Comments1:200 in 1X PBS/1% BSA/0.3% Triton X-100, 16 hours 4C.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for NONO Antibody (NB100-1556) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

NONO Overexpression Lysate (Native)

Secondary Antibodies

 

Isotype Controls

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Discover related pathways, diseases and genes to NONO Antibody (NB100-1556). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for NONO Antibody (NB100-1556)

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Recent Reviews

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Western Blot NONO NB100-1556
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 Anonymous
03/09/2018
Application: WB
Species: Human
read this review
Immunocytochemistry NONO NB100-1556
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 Anonymous
01/11/2018
Application: ICC
Species: Human
read this review

Bioinformatics

Gene Symbol NONO
Entrez
Uniprot