NONO Antibody

Western Blot: NONO Antibody [NB100-1556] - B-cell whole cell lysate 40ug. This image was submitted via customer review.

Immunocytochemistry: NONO Antibody [NB100-1556] - NONO in human fibroblasts at 1:200 dilution in 1X PBS/1% BSA/0.3% Triton X-100, 16 hours 4C. This image was submitted by customer review.

Immunohistochemistry-Paraffin: NONO Antibody [NB100-1556] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- NONO used at a dilution of 1:1,000 (0.2 ug/ml) and 1:200 (1ug/ml). Detection: DAB

Western Blot: NONO Antibody [NB100-1556] - Detection of Human NONO on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T cell lystate (50 microgram) using NB100-1556. NONO was also immunoprecipitated using anti-NONO antibody NB100-1554 or NB100-1555 at 3 microg/mg lysate.

Western Blot: NONO Antibody [NB100-1556] - Whole cell lysate NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells.

Proximity Ligation Assay: NONO Antibody [NB100-1556] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human NONO (NB100-1556). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

Proximity Ligation Assay: NONO Antibody [NB100-1556] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMARCA4 (NBP2-22234) and rabbit anti-human NONO (NB100-1556). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

Proximity Ligation Assay: NONO Antibody [NB100-1556] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human FUS (NBP2-14860) and rabbit anti-human NONO (NB100-1556). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

Proximity Ligation Assay: NONO Antibody [NB100-1556] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human POLR2A (NBP2-14859) and rabbit anti-human NONO (NB100-1556). Image merged from DAPI (2ms), FITC/Green (25ms) and TRITC/Orange (250ms) exposures, 40X magnification.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IHC-P, IP, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

NONO Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 350 and 400 of human Non-POU Domain Containing, Octamer-binding using the numbering given in entry NP_031389.3 (GeneID 4841).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NONO
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:200 - 1:1:000
  • Immunohistochemistry-Paraffin 1:200 - 1:1:000
  • Immunoprecipitation 1-4 ug/mg
  • Immunocytochemistry
• Application Notes: ICC reactivity per customer review.
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Positive Control:

  NONO Lysate (NBL1-13717)

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Orangutan.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NONO Antibody

• NMT5552 kDa subunit
• non-POU domain containing, octamer-binding
• NRB54non-POU-domain-containing, octamer-binding
• p54(nrb)

Background

Non-POU-domain-containing octamer binding protein (NONO) is a member of the DBHS (drosophila behavior, human splicing) domain-containing family and is an RNA- and DNA- binding protein. NONO and other DBHS domain-containing proteins are multifunctional and are reported to be involved in transcriptional regulation, mRNA processing, and DNA non-homologous end joining (NHEJ). NONO functions as a coregulator of the androgen receptor (AR) and also regulates cAMP transcriptional activity by interacting with gene promoter elements. NONO is also involved in pre-mRNA splicing through an interaction with U5 snRNA and can stimulate DNA nonhomologous end joining (NHEJ) through the interaction with ku70/G22p and ku80/XRCC5 dimers. Alternate names for NONO include 54 kDa nuclear RNA- and DNA-binding protein, p54 (nrb), 55 kDa nuclear protein, NMT55, DNA-binding p52/p100 complex, NRB54, P54, and P54NRB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NONO Antibody (NB100-1556)(3)

Publications using NB100-1556

• Krietsch J, Caron MC, Gagne JP et al. PARP activation regulates the RNA-binding protein NONO in the DNA damage response to DNA double-strand breaks. Nucleic Acids Res 2012 Nov [PMID: 22941645]
• Isabelle M, Gagne JP, Gallouzi IE et al. Quantitative proteomics and dynamic imaging reveal that G3BP-mediated stress granule assembly is poly(ADP-ribose)-dependent following exposure to MNNG-induced DNA alkylation. J Cell Sci 2012 Oct [PMID: 22767504]
• Zhang C, Zhang MX, Shen YH et al. TNF-alpha suppresses prolyl-4-hydroxylase alpha1 expression via the ASK1-JNK-NonO pathway. Arterioscler Thromb Vasc Biol 2007 Aug [PMID: 17478756]

Reviews for NONO Antibody (NB100-1556) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Anonymous WB Human 03/09/2018

Summary

• Application: Western Blot
• Sample Tested: B-cell whole cell lysate 40ug
• Species: Human
• Lot: A1

Comments

• Comments: BIOSCARF

reviewed by: Anonymous ICC Human 01/11/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: fibroblasts
• Species: Human
• Lot: A1

Comments

• Comments: 1:200 in 1X PBS/1% BSA/0.3% Triton X-100, 16 hours 4C.

Bioinformatics

• Gene Symbol: NONO
• Entrez:
  • 4841(Human)
• Uniprot:
  • Q15233 (Human)