GPIHBP1 Antibody

Western Blot: GPIHBP1 Antibody [NB110-41539] - Detection of mouse Gpihbp1 in transfected lysate (Lane 1). Empty vector lysate was used as a negative control (Lane 2).

Immunocytochemistry/Immunofluorescence: GPIHBP1 Antibody [NB110-41539] - GPIHBP1 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.9 mg/ml

GPIHBP1 Antibody Summary

• Immunogen: A synthetic peptide corresponding to an internal region [within residues 170-250] of mouse GPIHBP1. [Swiss-Prot# Q9D1N2]
• Localization: Plasma membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPIHBP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:20 - 1:50
  • Western Blot 2.0 ug/ml
• Application Notes: This GPIHBP1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. A flash ECL exposure is recommended for first try. In ICC/IF membrane staining was observed.
• Control:

  Mouse Kidney Whole Tissue Lysate (Adult Whole Normal)

• Control Peptide:

  GPIHBP1 Peptide (NB110-41539PEP)

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 0.9 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPIHBP1 Antibody

• glycosylphosphatidylinositol anchored high density lipoprotein binding protein1
• glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein1
• GPI anchored high density lipoprotein binding protein 1
• GPI-Anchored HDL-Binding Protein 1
• GPIHBP1
• GPI-HBP1
• GPI-HBP1LOC338328
• HBP1
• High density lipoprotein-binding protein 1
• HYPL1D

Background

GPIHBP1 (glycosylphosphatidylinositol-anchored HDL-binding protein 1) is a glycosylphosphatidylinositol-anchored glycoprotein of capillary endothelial cells that shuttle lipoprotein lipase (LPL) from the interstitial spaces to capillary lumen, and is essential for triglyceride-rich lipoproteins metabolism in mammalian plasma. GPIHBP1 is localized on luminal/abluminal capillary endothelial cell surfaces where it is bound by a glycosylphosphatidylinositol anchor and associates strongly with LPL. It serves as LPL transporter from sub-endothelial spaces to luminal face of capillaries, enabling lipolysis of circulating triglycerides localized within plasma chylomicrons. It has high affinity for HDL and binds to LPL, chylomicrons as well as APOA5. In the absence of GPIHBP1, the stores of catalytically active LPL within tissues are normal, but the LPL is mislocalized to interstitial spaces and is absent from capillary lumen. LPL mislocalization interferes with lipoprotein lipolysis and causes chylomicronemia. In humans, loss of function GPIHBP1 mutations leads to familial chylomicronemia. It binds LPL and apoA-V strongly for serving as a platform for lipolysis within capillaries, particularly in tissues which show high expression levels for both GPIHBP1 and LPL genes, such as heart, skeletal muscle and adipose tissue. Gpihbp1-/Gpihbp1- knock out mice have shown that GPIHBP1-deficiency causes severe hypertriglyceridemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for GPIHBP1 Antibody (NB110-41539). (Showing 1 - 1 of 1 FAQ).

1. Can you recommend a streptavidin 647 secondary for biotinylated GPIHBP1 antibody NB110-41539B? Also, can I use another primary rabbit IgG antibody with an anti-Rabbit IgG secondary in the 561 nm channel along with this biotinylated GPIHBP1 or will the rabbit IgG host isotype cause issues?
   • Ideally, you would not need a secondary for most applications. However, if you are looking for an anti-streptavidin that is conjugated with a "647" dye, I am afraid we do not have this dye available. If you are planning to use another antibody that is a rabbit polyclonal antibody, I would suggest not to use this pair, as this NB110-41539B is also rabbit polyclonal and it would cross react with the secondary antibody.

Bioinformatics

• Gene Symbol: GPIHBP1