HMGB1/HMG-1 Antibody [PE]


There are currently no images for HMGB1/HMG-1 Antibody (NB100-2322PE).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity Hu, Mu, Rt, Bv, Ca, Rb, ShSpecies Glossary
Applications Flow
Please see the vial label for concentration. If unlisted please contact technical services.

HMGB1/HMG-1 Antibody [PE] Summary

A synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100-200). [UniProt #P09429]
Predicted Species
Porcine (100%), Equine (100%)
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C in the dark.
PE (A=488, E=575)
0.05% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
Immunogen affinity purified


Application Notes
This HMGB1 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot, where a band is seen ~29 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
29 kDa

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 27401639). Predicted to react with porcine, and equine based on 100% sequence homology.

Alternate Names for HMGB1/HMG-1 Antibody [PE]

  • Amphoterin
  • high mobility group box 1
  • High mobility group protein 1
  • high mobility group protein B1
  • high-mobility group (nonhistone chromosomal) protein 1
  • high-mobility group box 1
  • HMG1
  • HMG-1
  • HMG1DKFZp686A04236
  • HMG3
  • SBP-1
  • Sulfoglucuronyl carbohydrate binding protein


HMGB1 (high mobility group protein B1) is a member of HMGB family and it has been extensively studied as a transcription/growth factor that is most ubiquitous and evolutionarily conserved protein in eukaryotic organisms. HMGB1 is localized in nucleus, however gets translocated to cytoplasm or may get secreted also. It is a DNA binding protein that preferentially binds with ssDNA and contains 2 HMG box DNA-binding domains with ability of chromatin association for bending DNA. HMGB1 implicates in in V(D)J recombination by acting as a cofactor of RAG complex that contains core components RAG1 and RAG2, as well as an associated component HMGB1 or HMGB2. HMGB1 acts by stimulating cleavage and RAG protein binding at 23bp spacer of conserved RSS (recombination signal sequences). Originally described as a nonhistone nuclear protein with transcriptional regulatory properties, HMGB1 (especially the cytoplasmic/secreted form) has also now been recognized as a late mediator in septic shock as well as a pro-inflammatory cytokine. It gets translocated to cytoplasm or secreted when cells get exposed to inflammatory mediators (LPS, TNF-alpha, IFN-gamma) or via passive process during cellular necrosis. HMGB1 has been implicated in the pathogenesis of various inflammatory and autoimmune diseases, such as SLE, RA and ankylosing spondylitis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Mu
Applications: Flow, ICC
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu, Ca
Applications: B/N, Flow, ICC/IF, IP, In vitro, Flow-CS, Flow-IC
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
Applications: Func-Inh
Species: Hu
Applications: WB
Species: Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ChIP, ICC/IF, IP
Species: Hu, Mu
Applications: WB, Simple Western
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Flow
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
Species: Hu, Mu, Rt, Bv, Ca, Rb, Sh, Equine, Porcine
Applications: Flow

Publications for HMGB1/HMG-1 Antibody (NB100-2322PE) (0)

There are no publications for HMGB1/HMG-1 Antibody (NB100-2322PE).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HMGB1/HMG-1 Antibody (NB100-2322PE) (0)

There are no reviews for HMGB1/HMG-1 Antibody (NB100-2322PE). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for HMGB1/HMG-1 Antibody (NB100-2322PE) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional HMGB1/HMG-1 Antibody [PE] Products

Related Products by Gene

Bioinformatics Tool for HMGB1/HMG-1 Antibody (NB100-2322PE)

Discover related pathways, diseases and genes to HMGB1/HMG-1 Antibody (NB100-2322PE). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HMGB1/HMG-1 Antibody (NB100-2322PE)

Discover more about diseases related to HMGB1/HMG-1 Antibody (NB100-2322PE).

Pathways for HMGB1/HMG-1 Antibody (NB100-2322PE)

View related products by pathway.

PTMs for HMGB1/HMG-1 Antibody (NB100-2322PE)

Learn more about PTMs related to HMGB1/HMG-1 Antibody (NB100-2322PE).

Research Areas for HMGB1/HMG-1 Antibody (NB100-2322PE)

Find related products by research area.

Blogs on HMGB1/HMG-1

There are no specific blogs for HMGB1/HMG-1, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol HMGB1

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB100-2322PE below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought