HMGB1/HMG-1 Antibody [DyLight 405]


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Product Details

Reactivity Hu, Mu, Rt, Bv, Ca, Rb, ShSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Please see the vial label for concentration. If unlisted please contact technical services.

HMGB1/HMG-1 Antibody [DyLight 405] Summary

A synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100-200). [UniProt #P09429]
Predicted Species
Porcine (100%), Equine (100%)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C in the dark.
DyLight 405
DyLight 405 (A=400, E=420)
50mM Sodium Borate
0.05% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
Immunogen affinity purified


Application Notes
This HMGB1 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot, where a band is seen ~29 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
29 kDa

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 27401639). Predicted to react with porcine, and equine based on 100% sequence homology.


Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for HMGB1/HMG-1 Antibody [DyLight 405]

  • Amphoterin
  • high mobility group box 1
  • High mobility group protein 1
  • high mobility group protein B1
  • high-mobility group (nonhistone chromosomal) protein 1
  • high-mobility group box 1
  • HMG1
  • HMG-1
  • HMG1DKFZp686A04236
  • HMG3
  • SBP-1
  • Sulfoglucuronyl carbohydrate binding protein


HMGB1 (high mobility group protein B1) is a member of HMGB family and it has been extensively studied as a transcription/growth factor that is most ubiquitous and evolutionarily conserved protein in eukaryotic organisms. HMGB1 is localized in nucleus, however gets translocated to cytoplasm or may get secreted also. It is a DNA binding protein that preferentially binds with ssDNA and contains 2 HMG box DNA-binding domains with ability of chromatin association for bending DNA. HMGB1 implicates in in V(D)J recombination by acting as a cofactor of RAG complex that contains core components RAG1 and RAG2, as well as an associated component HMGB1 or HMGB2. HMGB1 acts by stimulating cleavage and RAG protein binding at 23bp spacer of conserved RSS (recombination signal sequences). Originally described as a nonhistone nuclear protein with transcriptional regulatory properties, HMGB1 (especially the cytoplasmic/secreted form) has also now been recognized as a late mediator in septic shock as well as a pro-inflammatory cytokine. It gets translocated to cytoplasm or secreted when cells get exposed to inflammatory mediators (LPS, TNF-alpha, IFN-gamma) or via passive process during cellular necrosis. HMGB1 has been implicated in the pathogenesis of various inflammatory and autoimmune diseases, such as SLE, RA and ankylosing spondylitis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol HMGB1

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