SREBP2 Antibody

Images

 
Western Blot: SREBP2 Antibody [NB100-74543] - Analysis of 25 ug of mouse liver (lane 1), rat liver (lane 2) and HepG2 (lane 3) cell lysates.
Immunocytochemistry/ Immunofluorescence: SREBP2 Antibody [NB100-74543] - Analysis of SREBP2 (green) showing staining in the cytoplasm of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS ...read more
Immunocytochemistry/ Immunofluorescence: SREBP2 Antibody [NB100-74543] - Analysis of SREBP2 (green) showing staining in the cytoplasm of PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS ...read more
Immunocytochemistry/ Immunofluorescence: SREBP2 Antibody [NB100-74543] - Analysis of SREBP2 (green) showing staining in the cytoplasm of L6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SREBP2 Antibody Summary

Immunogen
Synthetic peptide corresponding to residues A(843) I S W L Q G D D A A V R S H(857) of rat SREBP 2.
Localization
Precursor form found in ER, shuttles to Golgi for activation by proteolytic cleavage, mature form in nucleus.
Specificity
SREBP2
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SREBF2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:20 - 1:200
Publications
Read Publications using
NB100-74543 in the following applications:

  • WB
    6 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (see J Biol Chem. 2007 Mar).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS containing 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SREBP2 Antibody

  • bHLhd2
  • bHLHd2sterol regulatory element-binding protein 2
  • Class D basic helix-loop-helix protein 2
  • SREBF2
  • SREBP2
  • SREBP2SREBP-2
  • sterol regulatory element binding transcription factor 2
  • Sterol regulatory element-binding transcription factor 2

Background

Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF

Publications for SREBP2 Antibody (NB100-74543)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(6)
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Filter By Species
Human
(1)
Mouse
(4)
All Species
Showing Publications 1 - 6 of 6.
Publications using NB100-74543 Applications Species
Kondo Yoshitaka, Masutomi Hirofumi, Noda Yoshihiro et al. Senescence marker protein-30/superoxide dismutase 1 double knockout mice exhibit increased oxidative stress and hepatic steatosis. FEBS Open Bio. 2014 May 24 [PMID: 25003023] (WB, Mouse) WB Mouse
Takahashi K, Kishimoto Y, Konishi T et al. Ascorbic acid deficiency affects genes for oxidation-reduction and lipid metabolism in livers from SMP30/GNL knockout mice. Biochim. Biophys. Acta 2014 Apr 03 [PMID: 24704458] (WB, Mouse) WB Mouse
Duan Y, Chen Y, Hu W et al. Peroxisome Proliferator-activated Receptor Activation by Ligands and Dephosphorylation Induces Proprotein Convertase Subtilisin Kexin Type 9 and Low Density Lipoprotein Receptor Expression J Biol Chem 2012 Jul 6 [PMID: 22593575] (WB, Human, Mouse) WB Human, Mouse
Slana I, Pribylova R, Kralova A, Pavlik I. Persistence of Mycobacterium avium subsp. paratuberculosis at a farm-scale biogas plant supplied with manure from paratuberculosis-affected dairy cattle. Appl Environ Microbiol 2011 May [PMID: 21398476] (WB) WB
Rabot S, Membrez M, Bruneau A et al. Germ-free C57BL/6J mice are resistant to high-fat-diet-induced insulin resistance and have altered cholesterol metabolism. FASEB J 2010 Dec [PMID: 20724524] (WB, Mouse) WB Mouse
Huster D, Purnat TD et al. High copper selectively alters lipid metabolism and cell cycle machinery in the mouse model of Wilson disease. J Biol Chem;282(11):8343-55. 2007 Mar 16. [PMID: 17205981] (WB) WB

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SREBP2 Antibody (NB100-74543) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on SREBP2.

SREBP2 - regulating cholesterol homeostasis and lipid metabolism
Sterol regulatory element-binding proteins (SREBP) are important transcription factors regulating the synthesis and uptake of lipids including cholesterol. This essential role in lipid metabolism makes investigations into the functions SREBPs im...  Read full blog post.

SREBP: Gatekeeper of Cholesterol Homeostasis
SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g....  Read full blog post.

SREBPs: Global Regulator of Lipid Metabolism
Sterol regulatory element binding proteins (SREBPs) are indirectly required for cholesterol biosynthesis and for uptake and fatty acid biosynthesis. There are three known SREBP isoforms, SREBP1a, 1c and SREBP2; these have different roles in lipid synt...  Read full blog post.

SREBP2: From Cholesterol Homeostasis to Cancer Invasion
Sterol-regulatory-element-binding protein 2 (SREBP2) is a transcription factor that regulates cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g. HMG-CoA. Along with another transcription factor LXR, SREBP...  Read full blog post.

ABCA1 Expression is Down-Regulated by SREBP microRNA
The ABCA1 (ATP-binding cassette transporter-A1) gene encodes a transmembrane protein, which plays a major role in phospholipid homeostasis by regulating cholesterol efflux from the cell. ABCA1 antibody studies have shown ABCA1 expression is up/down re...  Read full blog post.

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Bioinformatics

Gene Symbol SREBF2
Entrez
Uniprot