FDPS Antibody

Western Blot: FDPS Antibody [NBP2-16463] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FDPS antibody [N1C3] diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: FDPS Antibody [NBP2-16463] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: FDPS protein stained by FDPS antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: FDPS Antibody [NBP2-16463] - Mouse kidney. FDPS stained by FDPS antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: FDPS Antibody [NBP2-16463] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:3000.

Western Blot: FDPS Antibody [NBP2-16463] - A. 50 ug rat liver lysate/extract 10% SDS-PAGE FDPS antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Simple Western: FDPS Antibody [NBP2-16463] - SVG-A whole cell lysate. Antibody at 1:25. Image from verified customer review.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

FDPS Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human FDPS. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Bovine (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FDPS
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:1000
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
  • Simple Western
  • Western Blot 1:500-1:3000
• Application Notes: FDPS antibody validated for Simple Western from a verified customer review.
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Porcine (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol (pH7).
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for FDPS Antibody

• 6E)-farnesyl diphosphate synthase
• Dimethylallyltranstransferase
• EC 2.5.1.1
• EC 2.5.1.10
• farnesyl diphosphate synthaseFPP synthetase
• farnesyl pyrophosphate synthase
• farnesyl pyrophosphate synthetase, dimethylallyltranstransferase
• FDPS
• FPP synthase
• FPPS
• FPSgeranyltranstransferase
• Geranyltranstransferase
• KIAA1293

Background

This gene encodes an enzyme that catalyzes the production of geranyl pyrophosphate and farnesyl pyrophosphate from isopentenyl pyrophosphate and dimethylallyl pyrophosphate. The resulting product, farnesyl pyrophosphate, is a key intermediate in cholesterol and sterol biosynthesis, a substrate for protein farnesylation and geranylgeranylation, and a ligand or agonist for certain hormone receptors and growth receptors. Drugs that inhibit this enzyme prevent the post-translational modifications of small GTPases and have been used to treat diseases related to bone resorption. Multiple pseudogenes have been found on chromosomes 1, 7, 14, 15, 21 and X. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Review for FDPS Antibody (NBP2-16463) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Julian Wong Simple Western Human 07/03/2018

Summary

• Application: Simple Western
• Sample Tested: SVG-A whole cell lysate
• Species: Human
• Lot: 40261

Bioinformatics

• Gene Symbol: FDPS
• Uniprot:
  • P14324(Human)