Heparanase/HPSE Antibody - BSA Free

Western Blot: Heparanase/HPSE Antibody [NBP3-03846] - Analysis of extracts of various cells, using Heparanase/HPSE antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry/ Immunofluorescence: Heparanase/HPSE Antibody - Azide and BSA Free [Heparanase/HPSE] - Immunofluorescence analysis of NIH/3T3 cells using Heparanase/HPSE Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: Heparanase/HPSE Antibody - Azide and BSA Free [Heparanase/HPSE] - Immunofluorescence analysis of HeLa cells using Heparanase/HPSE Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Heparanase/HPSE Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Heparanase/HPSE Antibody - BSA Free (NBP3-03846) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-Heparanase/HPSE Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 36-275 of human Heparanase/HPSE (NP_001092010.1). QDVVDLDFFTQEPLHLVSPSFLSVTIDANLATDPRFLILLGSPKLRTLARGLSPAYLRFGGTKTDFLIFDPKKESTFEERSYWQSQVNQDICKYGSIPPDVEEKLRLEWPYQEQLLLREHYQKKFKNSTYSRSSVDVLYTFANCSGLDLIFGLNALLRTADLQWNSSNAQLLLDYCSSKGYNISWELGNEPNSFLKKADIFINGSQLGEDFIQLHKLLRKSTFKNAKLYGPDVGQPRRKT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HPSE
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for Heparanase/HPSE Antibody - BSA Free

• EC 3.2
• Endo-glucoronidase
• HEP
• Heparanase
• Heparanase-1
• HPA1
• HPAheparanase exon 9 and 10 deletion
• HPR1
• HPSE
• HPSE1heparanase-1
• HSE1
• HSE1heparanase exon10-deletion

Background

Heparanase is an endo-beta-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasations of tumor cells and inflammatory leukocytes. Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis. Heparanase is a heterodimer comprised of a 50 kDa subunit harboring the active site and an 8 kDa subunit. It is produced as a latent 65 kDa precursor and proteolytically processed to its active form. Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Publications for Heparanase/HPSE Antibody (NBP3-03846)(1)

Publication using NBP3-03846

• Sheren Farrag Mahmoud Ahmed, Mona Mohammed Abdelrahman, Maisa Hashem Mohammed Evaluation of Immunohistochemical Expression of Heparanase in Helicobacter pylori -Associated Chronic Gastritis Clinical Pathology 2023-07-31 [PMID: 37534373]

Bioinformatics

• Gene Symbol: HPSE