Glutaminase Antibody

Images

 
Western Blot: Glutaminase Antibody [NBP1-89766] - Analysis in human cell line EFO-21.
Immunocytochemistry/ Immunofluorescence: Glutaminase Antibody [NBP1-89766] - Staining of human cell line SiHa shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Glutaminase Antibody [NBP1-89766] - Staining in human kidney and skeletal muscle tissues using NBP1-89766 antibody. Corresponding GLS RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Glutaminase Antibody [NBP1-89766] - Staining of human cerebral cortex shows moderate to strong granular cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: Glutaminase Antibody [NBP1-89766] - Staining of human duodenum shows moderate granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Glutaminase Antibody [NBP1-89766] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: Glutaminase Antibody [NBP1-89766] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Glutaminase Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DRWNNTPMDEALHFGHHDVFKILQEYQVQYTPQGDSDNGKENQTVHKNLDGL
Specificity
Specificity of human Glutaminase antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04 - 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Glutaminase Protein (NBP1-89766PEP)
Publications
Read Publications using
NBP1-89766 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25798620). Rat reactivity reported in scientific literature (PMID: 31265816).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Glutaminase Antibody

  • AAD20
  • EC 3.5.1.2
  • GLS
  • GLS1DKFZp686O15119
  • glutaminase C
  • glutaminase kidney isoform, mitochondrial
  • Glutaminase
  • glutaminase, phosphate-activated
  • K-Glutaminase
  • KIAA0838FLJ10358
  • L-Glutamine Amidohydrolase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glutaminase Antibody (NBP1-89766)(2)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: IHC, WB.


Filter By Application
IHC
(1)
WB
(1)
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Filter By Species
Mouse
(1)
Rat
(1)
All Species

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol GLS