Glutamate Dehydrogenase Antibody

Images

 
Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamate Dehydrogenase antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Glutamate Dehydrogenase Antibody [NBP2-16679] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Glutamate Dehydrogenase protein stained by Glutamate Dehydrogenase ...read more
Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679] - Paraffin-embedded rat brain. Glutamate Dehydrogenase antibody diluted at 1:750.
Immunocytochemistry/ Immunofluorescence: Glutamate Dehydrogenase Antibody [NBP2-16679] - U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glutamate Dehydrogenase protein stained by Glutamate ...read more
Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Sh, XpSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.07 mg/ml

Order Details

Glutamate Dehydrogenase Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Glutamate Dehydrogenase. The exact sequence is proprietary.
Localization
Mitochondrion matrix
Marker
Mitochondria Marker
Predicted Species
Porcine (99%), Sheep (99%), Xenopus (91%), Bovine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLUD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-16679 in the following applications:

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (91%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7)
Preservative
0.025% Proclin 300
Concentration
0.07 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Glutamate Dehydrogenase Antibody

  • EC 1.4.1
  • EC 1.4.1.3
  • GDH 1
  • GDH
  • GDH1
  • GLUD
  • glutamate dehydrogenase (NAD(P)+)
  • Glutamate Dehydrogenase 1
  • glutamate dehydrogenase 1, mitochondrial
  • MGC132003

Background

L-glutamate dehydrogenase (EC 1.4.1.3) has a central role in nitrogen metabolism in plants and animals. Glutamate dehydrogenase is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate (Smith et al., 2001 [PubMed 11254391]). Glutamate, the main substrate of GLUD, is present in brain in concentrations higher than in other organs. In nervous tissue, GLUD appears to function in both the synthesis and the catabolism of glutamate and perhaps in ammonia detoxification (Mavrothalassitis et al., 1988 [PubMed 3368458]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glutamate Dehydrogenase Antibody (NBP2-16679)(3)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GLUD1
Entrez
Uniprot