Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] - Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Glutamate Dehydrogenase Antibody [NBP2-16679] - U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glutamate Dehydrogenase protein stained by Glutamate ...read more
Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679] - Paraffin-embedded rat brain. Glutamate Dehydrogenase antibody diluted at 1:750.
Immunocytochemistry/ Immunofluorescence: Glutamate Dehydrogenase Antibody [NBP2-16679] - Analysis of methanol-fixed HeLa, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
1XPBS, 1%BSA, 20% Glycerol (pH7).
Immunogen affinity purified
suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
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Alternate Names for Glutamate Dehydrogenase Antibody
glutamate dehydrogenase (NAD(P)+)
glutamate dehydrogenase 1
glutamate dehydrogenase 1, mitochondrial
L-glutamate dehydrogenase (EC 188.8.131.52) has a central role in nitrogen metabolism in plants and animals. Glutamate dehydrogenase is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate (Smith et al., 2001 [PubMed 11254391]). Glutamate, the main substrate of GLUD, is present in brain in concentrations higher than in other organs. In nervous tissue, GLUD appears to function in both the synthesis and the catabolism of glutamate and perhaps in ammonia detoxification (Mavrothalassitis et al., 1988 [PubMed 3368458]).[supplied by OMIM]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.