GLS2 Antibody


Western Blot: GLS2 Antibody [NBP1-76544] - Glutaminase protein expression in PC3 and PC3M cell lines before/after CB-839 treatment. PC3 & PC3M cells were treated (+) or not treated (-) with GLS1 inhibitor CB-839 (1 uM) more
Immunocytochemistry/ Immunofluorescence: GLS2 Antibody [NBP1-76544] - of GLS2 in Human Kidney cells. Dilution 20 ug/mL.
Immunohistochemistry: GLS2 Antibody [NBP1-76544] - Immunohistochemistry of GLS2 in human kidney tissue with GLS2 antibody at 5 ug/mL.
Simple Western: GLS2 Antibody [NBP1-76544] - Simple Western lane view shows a specific band for GLS2 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P
1 mg/ml

GLS2 Antibody Summary

Antibody was raised against a 18 amino acid peptide near the center terminus of human GLS2 (NP_037399).
Specificity for GLS2 versus GLS1 has not been determined.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 0.5-1 ug/ml
  • Simple Western 1:100
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
GLS2 Peptide (NBP1-76544PEP)
Read Publications using
NBP1-76544 in the following applications:

  • 1 publication
  • WB
    2 publications

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1 mg/ml
Immunogen affinity purified

Alternate Names for GLS2 Antibody

  • EC
  • GAbreast cell glutaminase
  • GLSglutaminase GA
  • glutaminase 2 (liver, mitochondrial)
  • glutaminase I
  • glutaminase liver isoform, mitochondrial
  • hLGA
  • LGA
  • L-glutaminase
  • L-glutamine amidohydrolase
  • MGC71567
  • phosphate-activated glutaminase
  • phosphate-dependent glutaminase
  • truncated glutaminase 2


Phosphate-activated glutaminase, also known as Glutaminase 2 (GLS2), was initially isolated from rat liver, although it has been shown to be expressed in other tissues. Like the functionally similar, larger kidney glutaminase, GLS2 catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Expression of GLS2 is increased by p53 under both stressed and nonstressed conditions, resulting in increased levels of glutamate and alpha-ketoglutarate, which in turn results in enhanced mitochondrial respiration and ATP generation. GLS2 also regulates antioxidant defense function in cells by increasing reduced glutathione levels and decreasing ROS-levels, suggesting that GLS2 acts as a mediator of p53's role in antioxidant defense in addition to its role in energy metabolism.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC
Species: Hu
Applications: IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Fe
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, IHC
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P

Publications for GLS2 Antibody (NBP1-76544)(3)

Reviews for GLS2 Antibody (NBP1-76544) (0)

There are no reviews for GLS2 Antibody (NBP1-76544). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GLS2 Antibody (NBP1-76544). (Showing 1 - 2 of 2 FAQs).

  1. I was wondering if you had any available references for GLS2 antibody?
    • Unfortunately we are not aware of any references for this product at this time; however all of the species and applications listed on the datasheet are 100% guaranteed.
  2. What is the sequence which NBP1-76544 would recognize? It is described as "a 18 amino acid peptide near the center terminus of human GLS2" but may we ask if the specific sequence can be shared?
    • The exact sequence of NBP1-76544 is proprietary, but it is from within amino acids 301-360 of NP_037399.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-76544AF350
Alexa Fluor 405 NBP1-76544AF405
Alexa Fluor 488 NBP1-76544AF488
Alexa Fluor 532 NBP1-76544AF532
Alexa Fluor 594 NBP1-76544AF594
Alexa Fluor 647 NBP1-76544AF647
Alexa Fluor 700 NBP1-76544AF700
Alexa Fluor 750 NBP1-76544AF750
Biotin NBP1-76544B
DyLight 350 NBP1-76544UV
DyLight 405 NBP1-76544V
DyLight 488 NBP1-76544G
DyLight 550 NBP1-76544R
DyLight 594 NBP1-76544DL594
DyLight 650 NBP1-76544C
DyLight 680 NBP1-76544FR
DyLight 755 NBP1-76544IR
FITC NBP1-76544F
HRP NBP1-76544H
Janelia Fluor 549 NBP1-76544JF549
Janelia Fluor 646 NBP1-76544JF646

Additional GLS2 Products

Bioinformatics Tool for GLS2 Antibody (NBP1-76544)

Discover related pathways, diseases and genes to GLS2 Antibody (NBP1-76544). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GLS2 Antibody (NBP1-76544)

Discover more about diseases related to GLS2 Antibody (NBP1-76544).

Pathways for GLS2 Antibody (NBP1-76544)

View related products by pathway.

PTMs for GLS2 Antibody (NBP1-76544)

Learn more about PTMs related to GLS2 Antibody (NBP1-76544).

Blogs on GLS2

There are no specific blogs for GLS2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our GLS2 Antibody and receive a gift card or discount.


Gene Symbol GLS2