Smoothened Antibody

Images

 
Immunohistochemistry-Paraffin: Smoothened Antibody [NLS2666] - Analysis of anti-Smoothened antibody with human kidney.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bt, Bv, Ca, Eq, Ha, Pm, Rb, Ma-Op, PmSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Smoothened Antibody Summary

Immunogen
Synthetic 15 amino acid peptide from N-terminal extracellular domain of human SMO.
Specificity
Human Smoothened. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Species
Opossum (93%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMO
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
Control
Smoothened Knockout 293T Cell Lysate
Publications
Read Publication using
NLS2666 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%), Turkey (87%), Chicken (87%), Xenopus (87%), Zebrafish (80%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Smoothened Antibody

  • bnb
  • CRJS
  • FZD11
  • Gx
  • Protein Gx
  • SMO
  • SMOH
  • SMOHseven transmembrane helix receptor
  • smoothened (Drosophila) homolog
  • smoothened homolog (Drosophila)
  • smoothened homolog
  • Smoothened

Background

Smoothened, a Frizzled Receptor, is homologous to the Drosophila segment polarity Smo gene. It associates with the Patched protein to mediate the cellular response to the Hedgehog secreted protein signal, which controls patterning and growth during vertebrate development. Smoothened is activated in a number of human tumors and can function as an oncogene in basal-cell carcinomas. Smoothened expression has been documented in bladder, blood, brain, the central nervous system, skin, and ureter. ESTs have been isolated from amnion, bone, brain, breast, embryo, eye, heart, nerve, and skin libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NLS2666
Species: Hu, Mu, Rt, Po, Bt, Bv, Ca, Eq, Ha, Pm, Rb
Applications: ICC/IF, IHC, IHC-P
NBP1-57016
Species: Hu
Applications: WB
H00003077-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, S-ELISA
NBP1-71662
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NB600-600
Species: Hu, Mu, Ca
Applications: WB, ChIP, ELISA, Flow, IB, IHC, IHC-P, Flow-IC, KD
AF482
Species: Mu
Applications: WB, IHC
NBP1-33581
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P
NBP2-80532
Species: Hu, Pm
Applications: WB, IHC, IHC-P
NBP1-85351
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF3635
Species: Mu
Applications: WB, IHC
NBP2-55775
Species: Hu
Applications: WB, ICC/IF
AF3690
Species: Hu, Mu
Applications: WB, ChIP, ICC
NBP2-20536
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
AF1568
Species: Mu
Applications: WB
H00027148-D01P
Species: Hu, Mu
Applications: WB, PLA
NBP1-92172
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NLS2666
Species: Hu, Mu, Rt, Po, Bt, Bv, Ca, Eq, Ha, Pm, Rb, Ma-Op, Pm
Applications: ICC/IF, IHC, IHC-P

Publications for Smoothened Antibody (NLS2666)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Reviews for Smoothened Antibody (NLS2666) (0)

There are no reviews for Smoothened Antibody (NLS2666). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Smoothened Antibody (NLS2666). (Showing 1 - 1 of 1 FAQ).

  1. Please let us know the best substitute product for Smoothened Antibody (NBP2-24543 ) which got discontinued?
    • Based on the species and applications that Smoothened antibody (NBP2-24543 ) was suggested for, the possible alternative would be NLS2666. The latter is a rabbit polyclonal that is good for WB and IHC-P applications in Human, mouse, and Primate species. 

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional Smoothened Products

Bioinformatics Tool for Smoothened Antibody (NLS2666)

Discover related pathways, diseases and genes to Smoothened Antibody (NLS2666). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Smoothened Antibody (NLS2666)

Discover more about diseases related to Smoothened Antibody (NLS2666).
 

Pathways for Smoothened Antibody (NLS2666)

View related products by pathway.

PTMs for Smoothened Antibody (NLS2666)

Learn more about PTMs related to Smoothened Antibody (NLS2666).
 

Research Areas for Smoothened Antibody (NLS2666)

Find related products by research area.

Blogs on Smoothened.

The role of Smoothened in pulmonary pathologies
The Hedgehog (Hh) family of secreted proteins is involved in a number of developmental processes, one of which is the development of cancer. Past data suggests that the Sonic hedgehog (Shh) receptor is composed of two transmembrane proteins, Patche...  Read full blog post.

Pancreatic Cancer Research Targets Hedgehog Signaling Pathway
The Hedgehog Signaling Pathway (HSP) is an important pathway involved in embryonic development by regulating cell differentiation. This pathway has also become an increasingly hot topic in cancer research in recent years. The HSP involves the interact...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Smoothened Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol SMO