Smoothened Antibody - BSA Free

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Immunohistochemistry-Paraffin: Smoothened Antibody [NLS2666] - Analysis of anti-Smoothened antibody with human kidney.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bt, Bv, Ca, Eq, Ha, Pm, Rb, PmSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Smoothened Antibody - BSA Free Summary

Description
Product can be stored undiluted at 4C for up to 1 month.
Immunogen
Synthetic 15 amino acid peptide from N-terminal extracellular domain of human Smoothened.
Epitope
N-Terminus extracellular domain
Specificity
Human Smoothened. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMO
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
Publications
Read Publication using
NLS2666 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%), Turkey (87%), Chicken (87%), Xenopus (87%), Zebrafish (80%).

Packaging, Storage & Formulations

Storage
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Smoothened Antibody - BSA Free

  • bnb
  • CRJS
  • FZD11
  • Gx
  • Protein Gx
  • SMO
  • SMOH
  • SMOHseven transmembrane helix receptor
  • smoothened (Drosophila) homolog
  • smoothened homolog (Drosophila)
  • smoothened homolog
  • Smoothened

Background

Smoothened, a Frizzled Receptor, is homologous to the Drosophila segment polarity Smo gene. It associates with the Patched protein to mediate the cellular response to the Hedgehog secreted protein signal, which controls patterning and growth during vertebrate development. Smoothened is activated in a number of human tumors and can function as an oncogene in basal-cell carcinomas. Smoothened expression has been documented in bladder, blood, brain, the central nervous system, skin, and ureter. ESTs have been isolated from amnion, bone, brain, breast, embryo, eye, heart, nerve, and skin libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Smoothened Antibody (NLS2666)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Smoothened Antibody (NLS2666). (Showing 1 - 1 of 1 FAQ).

  1. Please let us know the best substitute product for Smoothened Antibody (NBP2-24543 ) which got discontinued?
    • Based on the species and applications that Smoothened antibody (NBP2-24543 ) was suggested for, the possible alternative would be NLS2666. The latter is a rabbit polyclonal that is good for WB and IHC-P applications in Human, mouse, and Primate species. 

Secondary Antibodies

 

Isotype Controls

Additional Smoothened Products

Research Areas for Smoothened Antibody (NLS2666)

Find related products by research area.

Blogs on Smoothened.

The role of Smoothened in pulmonary pathologies
The Hedgehog (Hh) family of secreted proteins is involved in a number of developmental processes, one of which is the development of cancer. Past data suggests that the Sonic hedgehog (Shh) receptor is composed of two transmembrane proteins, Patche...  Read full blog post.

Pancreatic Cancer Research Targets Hedgehog Signaling Pathway
The Hedgehog Signaling Pathway (HSP) is an important pathway involved in embryonic development by regulating cell differentiation. This pathway has also become an increasingly hot topic in cancer research in recent years. The HSP involves the interact...  Read full blog post.

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Bioinformatics

Gene Symbol SMO