Human Factor IX ELISA Kit (Colorimetric)

ELISA: Human Factor IX ELISA Kit (Colorimetric) [NBP2-60518] - Standard curve.

ELISA: Human Factor IX ELISA Kit (Colorimetric) [NBP2-60518] - Standard curve.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell Culture, CSF
• Standard Curve Range: 1.56 - 100 ng/mL; 0.313 - 20 mIU/mL (example only; lot dependent)
• Sensitivity: 0.4 ng/mL (example only; lot dependent)

Human Factor IX ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 1.56 - 100 ng/mL; 0.313 - 20 mIU/mL (example only; lot dependent)
• Sensitivity: 0.4 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 8.90 (example only; lot dependent)
• Intra-Assay: %CV < 3.50 (example only; lot dependent)
• Spike Recovery: 99.00% (example only; lot dependent)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: F9

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Factor IX ELISA Kit (Colorimetric)

• Christmas Factor
• Coagulation Factor IX
• EC 3.4.21
• EC 3.4.21.22
• F9
• factor 9
• FIX
• HEMB
• MGC129641
• MGC129642
• P19
• Plasma Thromboplastic Component
• Plasma thromboplastin component
• PTC
• THPH8

Background

Factor IX encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Factor IX ELISA Kit (NBP2-60518)(1)

Publication using NBP2-60518

• Fang, Y;Liu, Y;Chen, L;Wang, J;Zhang, J;Zhang, H;Tian, S;Zhang, A;Zhang, J;Zhang, JH;Wang, X;Yu, J;Chen, S; Cerebrospinal fluid markers of neuroinflammation and coagulation in severe cerebral edema and chronic hydrocephalus after subarachnoid hemorrhage: a prospective study Journal of neuroinflammation 2024-09-27 [PMID: 39334416]

FAQs for Factor IX ELISA Kit (NBP2-60518). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: F9