GLI-2 Antibody

Images

 
GLI‑2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse GLI‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3635) at 15 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

GLI-2 Antibody Summary

Immunogen
E. coli-derived recombinant mouse GLI-2
Met1-Glu416
Accession # NP_001074594
Specificity
Detects mouse GLI-2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GLI2
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF3635 in the following applications:

Publications
Read Publications using
AF3635 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GLI-2 Antibody

  • GLI family zinc finger 2
  • GLI2
  • GLI-2
  • GLI-Kruppel family member GLI2
  • glioma-associated oncogene family zinc finger 2
  • HPE9tax helper protein 1
  • oncogene GLI2
  • tax helper protein 2
  • Tax helper protein
  • tax-responsive element-2 holding protein
  • tax-responsive element-25-bp sequence binding protein
  • THP
  • THP1
  • THP2
  • zinc finger protein GLI2

Background

Mouse GLI-2 is a 185 kDa biopotential transcription regulator of the hedgehog signaling pathway. It contains 5 conserved tandem C2H2 zinc finger domains that are flanked by a repression domain at the N-terminal region and an activation domain in the C-terminal region. At least two additional isoforms that have amino acid substitutions/truncations in the region corresponding to amino acid residues 192‑350 of isoform 1 have been described. Transcriptional activity of GLI-2 is regulated by proteolytic processing and degradation. Within the region used as immunogen, mouse GLI-2 shares 91% and 97% amino acid sequence homology with that of human and rat GLI-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GLI-2 Antibody (AF3635)(17)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
IHC-P
(1)
Immunoprecipitation
(3)
Western Blot
(13)
All Applications
Filter By Species
Human
(3)
Mouse
(14)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using AF3635 Applications Species
J Sun, J Yoon, M Lee, HK Lee, YS Hwang, IO Daar Zic5 stabilizes Gli3 via a non-transcriptional mechanism during retinal development Cell Reports, 2022;38(5):110312. 2022 [PMID: 35108539] (Immunoprecipitation, Human) Immunoprecipitation Human
A Koeniger, A Brichkina, I Nee, L Dempwolff, A Hupfer, I Galperin, F Finkernage, A Nist, T Stiewe, T Adhikary, W Diederich, M Lauth Activation of Cilia-Independent Hedgehog/GLI1 Signaling as a Novel Concept for Neuroblastoma Therapy Cancers, 2021;13(8):. 2021 [PMID: 33921042] (Western Blot, Mouse) Western Blot Mouse
JM Dias, Z Alekseenko, A Jeggari, M Boareto, J Vollmer, M Kozhevniko, H Wang, MP Matise, A Alexeyenko, D Iber, J Ericson A Shh/Gli-driven three-node timer motif controls temporal identity and fate of neural stem cells Science Advances, 2020;6(38):. 2020 [PMID: 32938678] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
L Lospinoso, D Quaglio, I Basili, F Ghirga, F Bufalieri, M Caimano, S Balducci, M Moretti, I Romeo, E Loricchio, M Maroder, B Botta, M Mori, P Infante, L Di Marcotu A Smo/Gli Multitarget Hedgehog Pathway Inhibitor Impairs Tumor Growth Cancers (Basel), 2019;11(10):. 2019 [PMID: 31601026] (Western Blot, Mouse) Western Blot Mouse
A Martin-Hur, R Martin-Mor, N Robledinos, R Blanco, I Palacios-B, I Lastres-Be, A Cuadrado, FR Garcia-Gon NRF2-dependent gene expression promotes ciliogenesis and Hedgehog signaling Sci Rep, 2019;9(1):13896. 2019 [PMID: 31554934] (Western Blot, Mouse) Western Blot Mouse
P Ma, NN Song, Y Li, Q Zhang, L Zhang, L Zhang, Q Kong, L Ma, X Yang, B Ren, C Li, X Zhao, Y Li, Y Xu, X Gao, YQ Ding, B Mao Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning Cell Rep, 2019;28(2):541-553.e4. 2019 [PMID: 31291587] (Western Blot, Human) Western Blot Human
P Infante, R Faedda, F Bernardi, F Bufalieri, L Lospinoso, R Alfonsi, D Mazzà, M Siler, S Coni, A Po, M Petroni, E Ferretti, M Mori, E De Smaele, G Canettieri, C Capalbo, M Maroder, I Screpanti, M Kool, SM Pfister, D Guardavacc, A Gulino, L Di Marcotu Itch/?-arrestin2-dependent non-proteolytic ubiquitylation of SuFu controls Hedgehog signalling and medulloblastoma tumorigenesis Nat Commun, 2018;9(1):976. 2018 [PMID: 29515120] (Western Blot, Mouse) Western Blot Mouse
T Rosengren, LJ Larsen, LB Pedersen, ST Christense, LB Møller TSC1 and TSC2 regulate cilia length and canonical Hedgehog signaling via different mechanisms Cell. Mol. Life Sci., 2018;0(0):. 2018 [PMID: 29396625] (Western Blot, Mouse) Western Blot Mouse
VJ Guen, TE Chavarria, C Kröger, X Ye, RA Weinberg, JA Lees EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 29158396] (IHC-P, Mouse) IHC-P Mouse
J Li, Y Cui, J Xu, Q Wang, X Yang, Y Li, X Zhang, M Qiu, Z Zhang, Z Zhang Suppressor of Fused Restraint of Hedgehog Activity Level is Critical for Osteogenic Proliferation and Differentiation during Calvarial Bone Development J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28794157] (Western Blot, Mouse) Western Blot Mouse
Show All 17 Publications.

Reviews for GLI-2 Antibody (AF3635) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF3635:
Filter by Applications
WB
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot GLI-2 AF3635
Enlarge
4
reviewed by:
Ganesh Pusapati
WB Mouse 11/13/2015
View

Summary

ApplicationWestern Blot
Sample TestedNIH-3T3 cells
SpeciesMouse
Immunofluorescence GLI-2 AF3635
Enlarge
4
reviewed by:
Ganesh Pusapati
IF Mouse 11/13/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedNIH-3T3 cells
SpeciesMouse

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FAQs for GLI-2 Antibody (AF3635) (0)

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Secondary Antibodies

 

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Ganesh Pusapati
11/13/2015
Application: WB
Species: Mouse

Ganesh Pusapati
11/13/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol GLI2
Uniprot