GLI-2 Antibody

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GLI‑2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse GLI‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3635) at 15 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

GLI-2 Antibody Summary

Immunogen
E. coli-derived recombinant mouse GLI-2
Met1-Glu416
Accession # NP_001074594
Specificity
Detects mouse GLI-2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GLI2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF3635 in the following applications:

Publications
Read Publications using
AF3635 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GLI-2 Antibody

  • GLI family zinc finger 2
  • GLI2
  • GLI-2
  • GLI-Kruppel family member GLI2
  • glioma-associated oncogene family zinc finger 2
  • HPE9tax helper protein 1
  • oncogene GLI2
  • tax helper protein 2
  • Tax helper protein
  • tax-responsive element-2 holding protein
  • tax-responsive element-25-bp sequence binding protein
  • THP
  • THP1
  • THP2
  • zinc finger protein GLI2

Background

Mouse GLI-2 is a 185 kDa biopotential transcription regulator of the hedgehog signaling pathway. It contains 5 conserved tandem C2H2 zinc finger domains that are flanked by a repression domain at the N-terminal region and an activation domain in the C-terminal region. At least two additional isoforms that have amino acid substitutions/truncations in the region corresponding to amino acid residues 192‑350 of isoform 1 have been described. Transcriptional activity of GLI-2 is regulated by proteolytic processing and degradation. Within the region used as immunogen, mouse GLI-2 shares 91% and 97% amino acid sequence homology with that of human and rat GLI-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GLI-2 Antibody (AF3635)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: IHC-P, IP, WB, Western Blot.


Filter By Application
IHC-P
(1)
IP
(1)
WB
(7)
Western Blot
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(9)
All Species
Showing Publications 1 - 10 of 10.
Publications using AF3635 Applications Species
P Infante, R Faedda, F Bernardi, F Bufalieri, L Lospinoso, R Alfonsi, D Mazzà, M Siler, S Coni, A Po, M Petroni, E Ferretti, M Mori, E De Smaele, G Canettieri, C Capalbo, M Maroder, I Screpanti, M Kool, SM Pfister, D Guardavacc, A Gulino, L Di Marcotu Itch/?-arrestin2-dependent non-proteolytic ubiquitylation of SuFu controls Hedgehog signalling and medulloblastoma tumorigenesis Nat Commun, 2018;9(1):976. 2018 [PMID: 29515120] (WB,, Mouse) WB Mouse
VJ Guen, TE Chavarria, C Kröger, X Ye, RA Weinberg, JA Lees EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 29158396] (IHC-P,, Mouse) IHC-P Mouse
J Li, Y Cui, J Xu, Q Wang, X Yang, Y Li, X Zhang, M Qiu, Z Zhang, Z Zhang Suppressor of Fused Restraint of Hedgehog Activity Level is Critical for Osteogenic Proliferation and Differentiation during Calvarial Bone Development J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28794157] (WB,, Mouse) WB Mouse
Y Han, Y Xiong, X Shi, J Wu, Y Zhao, J Jiang Regulation of Gli ciliary localization and Hedgehog signaling by the PY-NLS/karyopherin-?2 nuclear import system PLoS Biol., 2017;15(8):e2002063. 2017 [PMID: 28777795] (WB,, Mouse) WB Mouse
H Cai, A Liu Spop regulates Gli3 activity and Shh signaling in dorsoventral patterning of the mouse spinal cord Dev. Biol., 2017;0(0):. 2017 [PMID: 28412462] (WB,, Mouse) WB Mouse
Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):. 2016 [PMID: 27930311] (Western Blot,, Human) Western Blot Human
Ciliary Entry of the Hedgehog Transcriptional Activator Gli2 Is Mediated by the Nuclear Import Machinery but Differs from Nuclear Transport in Being Imp-?/?1-Independent PLoS ONE, 2016;11(8):e0162033. 2016 [PMID: 27579771] (WB,, Mouse) WB Mouse
Kramann R, Fleig S, Schneider R, Fabian S, DiRocco D, Maarouf O, Wongboonsin J, Ikeda Y, Heckl D, Chang S, Rennke H, Waikar S, Humphreys B Pharmacological GLI2 inhibition prevents myofibroblast cell-cycle progression and reduces kidney fibrosis. J Clin Invest, 2015;125(8):2935-51. 2015 [PMID: 26193634] (WB,, Mouse) WB Mouse
Liu Y, Tsai I, Morleo M, Oh E, Leitch C, Massa F, Lee B, Parker D, Finley D, Zaghloul N, Franco B, Katsanis N Ciliopathy proteins regulate paracrine signaling by modulating proteasomal degradation of mediators. J Clin Invest, 2014;124(5):2059-70. 2014 [PMID: 24691443] (WB,, Mouse) WB Mouse
Humke EW, Dorn KV, Milenkovic L, Scott MP, Rohatgi R The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins. Genes Dev., 2010;24(7):670-82. 2010 [PMID: 20360384] (IP,, Mouse) IP Mouse

Reviews for GLI-2 Antibody (AF3635) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF3635:
Filter by Applications
WB
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot GLI-2 AF3635
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reviewed by:
Anonymous
WB Mouse 11/13/2015
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Summary

ApplicationWestern Blot
Sample TestedNIH-3T3 cells
SpeciesMouse

Blocking

Blocking Details5% milk in 1xTBS-Tween (0.1%)

Primary Anitbody

Dilution Ratio1:200 dilution in blocking buffer; incubation at 4 deg rotator for 16 hr

Secondary Antibody

Secondary DescriptionPeroxidase AffiniPure Donkey Anti-Goat IgG (H+L)
Secondary Manufacturer Cat#705-035-003
Secondary Concentration1:10,000 dilution

Details

Detection NotesECL pico; exposure time was 5 min; Amersham Hyperfilm ECL was used

Comments

CommentsNIH-3T3 cells was seeded on 6 cm plates. Confluent cells were serum starved overnight and then left untreated or treated with Sonic hedgehog conditioned medium for 2 hr. Nuclear and cytoplasmic fractions were prepared and equal volumes were loaded for Western Blot analysis of Gli2 using AF3635. The antibody gives a weak signal but it was specific (considering the size of Gli2) and as published in previous papers, Gli2 nicely translocate to nucleus after Sonic hedgehog treatment.
Immunofluorescence GLI-2 AF3635
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reviewed by:
Anonymous
IF Mouse 11/13/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedNIH-3T3 cells
SpeciesMouse

Blocking

Blocking Details2% donkey serum + 0.2% triton X-100, in 1xPBS; incubation was done for 30 min at room temperature

Primary Anitbody

Dilution Ratio1:20; 1 hr at room temperature; antibody was diluted in blocking buffer

Secondary Antibody

Secondary DescriptionDonkey anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate
Secondary Manufacturer Cat#A-11055
Secondary Concentration1:1000 dilution

Details

Detection NotesConfocal Microscopy using Leica TCS SP8; 488 laser was used for excitation; 63X oil immersion objective was used
Fixation Details4% formaldehyde
Wash Descriptionquick washes with 1X PBS (5 ml of 1x PBS was rapidly flown on coverslips)

Comments

CommentsNIH-3T3 cells was seeded on glass coverslips coated with poly-lysine. Confluent cells were serum starved overnight and then treated with a Hh agonist for 4 hr. The staining of Gli2 in these cells appears to be cytoplasmic and also enriched in nucleus.

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Recent Reviews

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5
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4
2
3
0
2
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1
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Anonymous
11/13/2015
Application: WB
Species: Mouse

Anonymous
11/13/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol GLI2
Entrez
Uniprot