Ret Antibody [Unconjugated]


Ret was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Mouse Ret Antigen Affinity-purified Polyclonal Antibody (Catalog # AF482) at 15 µg/mL overnight at 4 °C. Tissue was stained more

Product Details

Reactivity MuSpecies Glossary
Applications WB, IHC

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Ret Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant mouse Ret
Leu29-Arg637 (Phe174Ser)
Accession # P35546
Detects mouse Ret in direct ELISAs and Western blots.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Read Publications using
AF482 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ret Antibody [Unconjugated]

  • CDHF12MTC1
  • CDHR16
  • EC 2.7.10
  • Hirschsprung disease 1
  • Hirschsprung disease)
  • MEN2A
  • MEN2B
  • multiple endocrine neoplasia and medullary thyroid carcinoma 1
  • ret proto-oncogene
  • RET transforming sequence
  • Ret


The GDNF family of neurotrophic factors consititute a new family of factors within the TGF-beta superfamily. These proteins are potent survival factors for various central and peripheral neurons during development and in the adult animal. The GDNF family members (GDNF, neurturin and persephin) signal through multicomponent receptors that consist of the Ret receptor tyrosine kinase and one of four glycosyl-phosphatidylinositol (GPI)-linked ligand-binding subunits (GFR alpha ‑1 ‑ 4). GFR alpha -1, -2, and -4 are the preferred ligand-binding subunits for GDNF, neurturin and persephin, respectively. To date, the preferred ligand for GFR alpha ‑3 has not been identified. The Ret tyrosine-kinase receptor is encoded by the c-ret proto-oncogene. Mutations of the ret gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung’s disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma. Mouse Ret cDNA encodes a 1115 amino acid (aa) residue transmembrane tyrosine kinase with a 28 aa residue signal peptide, a 609 aa residue cysteine-rich extracellular domain and a 456 aa residue cytoplasmic domain. A cadherin-related sequence is also present in the extracellular domain of Ret. Human and mouse Ret share 83% amino acid sequence homology (77% homology in the extracellular domain and 93% homology in the cytoplasmic domain). Although Ret does not bind GDNF ligands directly, the extracellular domain of Ret binds the GDNF-GFR-alpha  complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha .

  1. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
  2. Enokido, Y. et al. (1998) Curr. Biol. 8:1019.
  3. Carlomagno, F. et al. (1998) Endocrinology, 139:3613.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ret Antibody (AF482)(28)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 6 applications: ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.

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Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using AF482 Applications Species
D Huzard, M Martin, F Maingret, J Chemin, F Jeanneteau, PF Mery, P Fossat, E Bourinet, A François The impact of C-tactile low-threshold mechanoreceptors on affective touch and social interactions in mice Oncogene, 2022-06-29;8(26):eabo7566. 2022-06-29 [PMID: 35767616] (IHC, Mouse) IHC Mouse
L Faure, Y Wang, ME Kastriti, P Fontanet, KKY Cheung, C Petitpré, H Wu, LL Sun, K Runge, L Croci, MA Landy, HC Lai, GG Consalez, A de Chevign, F Lallemend, I Adameyko, S Hadjab Single cell RNA sequencing identifies early diversity of sensory neurons forming via bi-potential intermediates Nat Commun, 2020-08-21;11(1):4175. 2020-08-21 [PMID: 32826903] (IHC, Mouse) IHC Mouse
Y Wang, H Wu, P Fontanet, S Codeluppi, N Akkuratova, C Petitpré, Y Xue-Franzé, K Niederreit, A Sharma, F Da Silva, G Comai, G Agirman, D Palumberi, S Linnarsson, I Adameyko, A Moqrich, A Schedl, G La Manno, S Hadjab, F Lallemend A cell fitness selection model for neuronal survival during development Nat Commun, 2019-09-12;10(1):4137. 2019-09-12 [PMID: 31515492] (ICC, Mouse) ICC Mouse
D Acton, X Ren, S Di Costanz, A Dalet, S Bourane, I Bertocchi, C Eva, M Goulding Spinal Neuropeptide Y1 Receptor-Expressing Neurons Form an Essential Excitatory Pathway for Mechanical Itch Cell Rep, 2019-07-16;28(3):625-639.e6. 2019-07-16 [PMID: 31315043] (IHC-Fr, Mouse) IHC-Fr Mouse
L Bartesaghi, Y Wang, P Fontanet, S Wanderoy, F Berger, H Wu, N Akkuratova, F Bouçanova, JJ Médard, C Petitpré, MA Landy, MD Zhang, P Harrer, C Stendel, R Stucka, M Dusl, ME Kastriti, L Croci, HC Lai, GG Consalez, A Pattyn, P Ernfors, J Senderek, I Adameyko, F Lallemend, S Hadjab, R Chrast PRDM12 Is Required for Initiation of the Nociceptive Neuron Lineage during Neurogenesis Cell Rep, 2019-03-26;26(13):3484-3492.e4. 2019-03-26 [PMID: 30917305] (IHC, Mouse) IHC Mouse
SC Lu, YS Chang, HW Kan, YL Hsieh Tumor necrosis factor-? mediated pain hypersensitivity through Ret receptor in resiniferatoxin neuropathy Kaohsiung J. Med. Sci., 2018-09-01;34(9):494-502. 2018-09-01 [PMID: 30173779] (IHC-P, Mouse) IHC-P Mouse
HJ Park, EC Bolton RET-mediated glial cell line derived neurotrophic factor signaling inhibits mouse prostate development Development, 2017-05-15;0(0):. 2017-05-15 [PMID: 28506996] (IHC, Western Blot, Mouse) IHC, Western Blot Mouse
JL Trevaskis, CB Sacramento, H Jouihan, S Ali, J Le Lay, S Oldham, N Bhagroo, BB Boland, J Cann, Y Chang, T O'Day, V Howard, C Reers, MS Winzell, DM Smith, M Feigh, P Barkholt, K Schreiter, M Austen, U Andag, S Thompson, L Jermutus, MP Coghlan, J Grimsby, C Dohrmann, CJ Rhodes, CM Rondinone, A Sharma Neurturin and a Glp-1 Analogue Act Synergistically to Alleviate Diabetes in Zucker Diabetic Fatty Rats Diabetes, 2017-04-13;0(0):. 2017-04-13 [PMID: 28408435] (IHC, Rat) IHC Rat
L Urien, S Gaillard, L Lo Re, P Malapert, M Bohic, A Reynders, A Moqrich Genetic ablation of GINIP-expressing primary sensory neurons strongly impairs Formalin-evoked pain Sci Rep, 2017-02-27;7(0):43493. 2017-02-27 [PMID: 28240741] (IHC-Fr, Mouse) IHC-Fr Mouse
NA Gabreski, JK Vaghasia, SS Novakova, NQ McDonald, BA Pierchala Exon Skipping in the RET Gene Encodes Novel Isoforms That Differentially Regulate RET Protein Signal Transduction J. Biol. Chem., 2016-05-23;291(31):16249-62. 2016-05-23 [PMID: 27226544] (Western Blot, Mouse) Western Blot Mouse
Show All 28 Publications.

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Gene Symbol RET