Ret Antibody [Unconjugated]

Ret was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Mouse Ret Antigen Affinity-purified Polyclonal Antibody (Catalog # AF482) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the ventral horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Ret Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Ret
  Leu29-Arg637 (Phe174Ser)
  Accession # P35546
• Specificity: Detects mouse Ret in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RET
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ret Antibody [Unconjugated]

• CDHF12MTC1
• CDHR16
• EC 2.7.10
• Hirschsprung disease 1
• Hirschsprung disease)
• MEN2A
• MEN2B
• multiple endocrine neoplasia and medullary thyroid carcinoma 1
• ret proto-oncogene
• RET transforming sequence
• Ret

Background

The GDNF family of neurotrophic factors consititute a new family of factors within the TGF-beta superfamily. These proteins are potent survival factors for various central and peripheral neurons during development and in the adult animal. The GDNF family members (GDNF, neurturin and persephin) signal through multicomponent receptors that consist of the Ret receptor tyrosine kinase and one of four glycosyl-phosphatidylinositol (GPI)-linked ligand-binding subunits (GFR alpha ‑1 ‑ 4). GFR alpha -1, -2, and -4 are the preferred ligand-binding subunits for GDNF, neurturin and persephin, respectively. To date, the preferred ligand for GFR alpha ‑3 has not been identified. The Ret tyrosine-kinase receptor is encoded by the c-ret proto-oncogene. Mutations of the ret gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung’s disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma. Mouse Ret cDNA encodes a 1115 amino acid (aa) residue transmembrane tyrosine kinase with a 28 aa residue signal peptide, a 609 aa residue cysteine-rich extracellular domain and a 456 aa residue cytoplasmic domain. A cadherin-related sequence is also present in the extracellular domain of Ret. Human and mouse Ret share 83% amino acid sequence homology (77% homology in the extracellular domain and 93% homology in the cytoplasmic domain). Although Ret does not bind GDNF ligands directly, the extracellular domain of Ret binds the GDNF-GFR-alpha  complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha .

1. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
2. Enokido, Y. et al. (1998) Curr. Biol. 8:1019.
3. Carlomagno, F. et al. (1998) Endocrinology, 139:3613.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ret Antibody (AF482)(28)

Publications using AF482

• D Huzard, M Martin, F Maingret, J Chemin, F Jeanneteau, PF Mery, P Fossat, E Bourinet, A François The impact of C-tactile low-threshold mechanoreceptors on affective touch and social interactions in mice Oncogene, 2022-06-29;8(26):eabo7566. 2022-06-29 [PMID: 35767616] (IHC, Mouse)
• L Faure, Y Wang, ME Kastriti, P Fontanet, KKY Cheung, C Petitpré, H Wu, LL Sun, K Runge, L Croci, MA Landy, HC Lai, GG Consalez, A de Chevign, F Lallemend, I Adameyko, S Hadjab Single cell RNA sequencing identifies early diversity of sensory neurons forming via bi-potential intermediates Nat Commun, 2020-08-21;11(1):4175. 2020-08-21 [PMID: 32826903] (IHC, Mouse)
• Y Wang, H Wu, P Fontanet, S Codeluppi, N Akkuratova, C Petitpré, Y Xue-Franzé, K Niederreit, A Sharma, F Da Silva, G Comai, G Agirman, D Palumberi, S Linnarsson, I Adameyko, A Moqrich, A Schedl, G La Manno, S Hadjab, F Lallemend A cell fitness selection model for neuronal survival during development Nat Commun, 2019-09-12;10(1):4137. 2019-09-12 [PMID: 31515492] (ICC, Mouse)
• D Acton, X Ren, S Di Costanz, A Dalet, S Bourane, I Bertocchi, C Eva, M Goulding Spinal Neuropeptide Y1 Receptor-Expressing Neurons Form an Essential Excitatory Pathway for Mechanical Itch Cell Rep, 2019-07-16;28(3):625-639.e6. 2019-07-16 [PMID: 31315043] (IHC-Fr, Mouse)
• L Bartesaghi, Y Wang, P Fontanet, S Wanderoy, F Berger, H Wu, N Akkuratova, F Bouçanova, JJ Médard, C Petitpré, MA Landy, MD Zhang, P Harrer, C Stendel, R Stucka, M Dusl, ME Kastriti, L Croci, HC Lai, GG Consalez, A Pattyn, P Ernfors, J Senderek, I Adameyko, F Lallemend, S Hadjab, R Chrast PRDM12 Is Required for Initiation of the Nociceptive Neuron Lineage during Neurogenesis Cell Rep, 2019-03-26;26(13):3484-3492.e4. 2019-03-26 [PMID: 30917305] (IHC, Mouse)
• SC Lu, YS Chang, HW Kan, YL Hsieh Tumor necrosis factor-? mediated pain hypersensitivity through Ret receptor in resiniferatoxin neuropathy Kaohsiung J. Med. Sci., 2018-09-01;34(9):494-502. 2018-09-01 [PMID: 30173779] (IHC-P, Mouse)
• HJ Park, EC Bolton RET-mediated glial cell line derived neurotrophic factor signaling inhibits mouse prostate development Development, 2017-05-15;0(0):. 2017-05-15 [PMID: 28506996] (IHC, Western Blot, Mouse)
• JL Trevaskis, CB Sacramento, H Jouihan, S Ali, J Le Lay, S Oldham, N Bhagroo, BB Boland, J Cann, Y Chang, T O'Day, V Howard, C Reers, MS Winzell, DM Smith, M Feigh, P Barkholt, K Schreiter, M Austen, U Andag, S Thompson, L Jermutus, MP Coghlan, J Grimsby, C Dohrmann, CJ Rhodes, CM Rondinone, A Sharma Neurturin and a Glp-1 Analogue Act Synergistically to Alleviate Diabetes in Zucker Diabetic Fatty Rats Diabetes, 2017-04-13;0(0):. 2017-04-13 [PMID: 28408435] (IHC, Rat)
• L Urien, S Gaillard, L Lo Re, P Malapert, M Bohic, A Reynders, A Moqrich Genetic ablation of GINIP-expressing primary sensory neurons strongly impairs Formalin-evoked pain Sci Rep, 2017-02-27;7(0):43493. 2017-02-27 [PMID: 28240741] (IHC-Fr, Mouse)
• NA Gabreski, JK Vaghasia, SS Novakova, NQ McDonald, BA Pierchala Exon Skipping in the RET Gene Encodes Novel Isoforms That Differentially Regulate RET Protein Signal Transduction J. Biol. Chem., 2016-05-23;291(31):16249-62. 2016-05-23 [PMID: 27226544] (Western Blot, Mouse)
• Elziere L, Sar C, Venteo S, Bourane S, Puech S, Sonrier C, Boukhadaoui H, Fichard A, Pattyn A, Valmier J, Carroll P, Mechaly I CaMKK-CaMK1a, a new post-traumatic signalling pathway induced in mouse somatosensory neurons. PLoS ONE, 2014-05-19;9(5):e97736. 2014-05-19 [PMID: 24840036] (IHC, Mouse)
• Gorokhova S, Gaillard S, Urien L, Malapert P, Legha W, Baronian G, Desvignes J, Alonso S, Moqrich A Uncoupling of molecular maturation from peripheral target innervation in nociceptors expressing a chimeric TrkA/TrkC receptor. PLoS Genet, 2014-02-06;10(2):e1004081. 2014-02-06 [PMID: 24516396] (IHC, Mouse)
• Gatto G, Dudanova I, Suetterlin P, Davies A, Drescher U, Bixby J, Klein R Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling. J Neurosci, 2013-03-20;33(12):5399-410. 2013-03-20 [PMID: 23516305] (IHC-Fr, Mouse)
• Vaegter CB, Jansen P, Fjorback AW Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling. Nat. Neurosci., 2010-11-21;14(1):54-61. 2010-11-21 [PMID: 21102451] (Western Blot, Mouse)
• Kawaguchi J, Nichols J, Gierl MS, Faial T, Smith A Isolation and propagation of enteric neural crest progenitor cells from mouse embryonic stem cells and embryos. Development, 2010-03-01;137(5):693-704. 2010-03-01 [PMID: 20147374] (ICC, Mouse)
• Milenkovic N, Frahm C, Gassmann M, Griffel C, Erdmann B, Birchmeier C, Lewin GR, Garratt AN Nociceptive tuning by stem cell factor/c-Kit signaling. Neuron, 2007-12-06;56(5):893-906. 2007-12-06 [PMID: 18054864] (ICC, Mouse)
• He Z, Jiang J, Kokkinaki M, Golestaneh N, Hofmann MC, Dym M Gdnf upregulates c-Fos transcription via the Ras/Erk1/2 pathway to promote mouse spermatogonial stem cell proliferation. Stem Cells, 2007-10-25;26(1):266-78. 2007-10-25 [PMID: 17962702] (ICC, Mouse)
• D'Autréaux F, Morikawa Y, Cserjesi P, Gershon MD Hand2 is necessary for terminal differentiation of enteric neurons from crest-derived precursors but not for their migration into the gut or for formation of glia. Development, 2007-05-16;134(12):2237-49. 2007-05-16 [PMID: 17507395] (IHC-P, Mouse)
• Yin L, Puliti A, Bonora E, Evangelisti C, Conti V, Tong WM, Medard JJ, Lavoue MF, Forey N, Wang LC, Manie S, Morel G, Raccurt M, Wang ZQ, Romeo G C620R mutation of the murine ret proto-oncogene: loss of function effect in homozygotes and possible gain of function effect in heterozygotes. Int. J. Cancer, 2007-07-15;121(2):292-300. 2007-07-15 [PMID: 17372903] (ICC, IHC-Fr, Mouse)
• Damon DH, Teriele JA, Marko SB Vascular-derived artemin: a determinant of vascular sympathetic innervation? Am. J. Physiol. Heart Circ. Physiol., 2007-03-02;293(1):H266-73. 2007-03-02 [PMID: 17337595] (ICC, Western Blot, Rat)
• Gossrau G, Thiele J, Konang R, Schmandt T, Brustle O Bone morphogenetic protein-mediated modulation of lineage diversification during neural differentiation of embryonic stem cells. Stem Cells, 2007-01-11;25(4):939-49. 2007-01-11 [PMID: 17218404] (ICC, Mouse)
• Igarashi Y, Aita M, Suzuki A, Nandasena T, Kawano Y, Nozawa-Inoue K, Maeda T Involvement of GDNF and its receptors in the maturation of the periodontal Ruffini endings. Neurosci. Lett., 2006-12-18;412(3):222-6. 2006-12-18 [PMID: 17178438] (IHC, Rat)
• Lindfors PH, Lindahl M, Rossi J, Saarma M, Airaksinen MS Ablation of persephin receptor glial cell line-derived neurotrophic factor family receptor alpha4 impairs thyroid calcitonin production in young mice. Endocrinology, 2006-02-23;147(5):2237-44. 2006-02-23 [PMID: 16497798] (IHC-Fr, Mouse)
• Hofmann MC, Braydich-Stolle L, Dettin L, Johnson E, Dym M Immortalization of mouse germ line stem cells. Stem Cells, 2005-02-01;23(2):200-10. 2005-02-01 [PMID: 15671143] (ICC, Mouse)
• Leitner ML, Wang LH, Osborne PA, Golden JP, Milbrandt J, Johnson EM Expression and function of GDNF family ligands and receptors in the carotid body. Exp. Neurol., 2005-02-01;191(0):S68-79. 2005-02-01 [PMID: 15629763] (ICC, Rat)
• Lee CS, Tee LY, Dusenbery S, Takata T, Golden JP, Pierchala BA, Gottlieb DI, Johnson EM, Choi DW, Snider BJ Neurotrophin and GDNF family ligands promote survival and alter excitotoxic vulnerability of neurons derived from murine embryonic stem cells. Exp. Neurol., 2005-01-01;191(1):65-76. 2005-01-01 [PMID: 15589513] (ICC, Mouse)
• Schuetz G, Rosario M, Grimm J, Boeckers TM, Gundelfinger ED, Birchmeier W The neuronal scaffold protein Shank3 mediates signaling and biological function of the receptor tyrosine kinase Ret in epithelial cells. J. Cell Biol., 2004-11-29;167(5):945-52. 2004-11-29 [PMID: 15569713] (Immunoprecipitation, Mouse)
• Tamura S, Morikawa Y, Senba E TRPV2, a capsaicin receptor homologue, is expressed predominantly in the neurotrophin-3-dependent subpopulation of primary sensory neurons. Neuroscience, 2005-01-01;130(1):223-8. 2005-01-01 [PMID: 15561438] (ICC, Mouse)

Bioinformatics

• Gene Symbol: RET
• Uniprot:
  • P35546()