Ret Antibody [Unconjugated]

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Ret was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Mouse Ret Antigen Affinity-purified Polyclonal Antibody (Catalog # AF482) at 15 µg/mL overnight at 4 °C. Tissue was stained ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Ret Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Ret
Leu29-Arg637 (Phe174Ser)
Accession # P35546
Specificity
Detects mouse Ret in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RET
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF482 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ret Antibody [Unconjugated]

  • CDHF12MTC1
  • CDHR16
  • EC 2.7.10
  • Hirschsprung disease 1
  • Hirschsprung disease)
  • MEN2A
  • MEN2B
  • multiple endocrine neoplasia and medullary thyroid carcinoma 1
  • ret proto-oncogene
  • RET transforming sequence
  • Ret

Background

The GDNF family of neurotrophic factors consititute a new family of factors within the TGF-beta superfamily. These proteins are potent survival factors for various central and peripheral neurons during development and in the adult animal. The GDNF family members (GDNF, neurturin and persephin) signal through multicomponent receptors that consist of the Ret receptor tyrosine kinase and one of four glycosyl-phosphatidylinositol (GPI)-linked ligand-binding subunits (GFR alpha ‑1 ‑ 4). GFR alpha -1, -2, and -4 are the preferred ligand-binding subunits for GDNF, neurturin and persephin, respectively. To date, the preferred ligand for GFR alpha ‑3 has not been identified. The Ret tyrosine-kinase receptor is encoded by the c-ret proto-oncogene. Mutations of the ret gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung’s disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma. Mouse Ret cDNA encodes a 1115 amino acid (aa) residue transmembrane tyrosine kinase with a 28 aa residue signal peptide, a 609 aa residue cysteine-rich extracellular domain and a 456 aa residue cytoplasmic domain. A cadherin-related sequence is also present in the extracellular domain of Ret. Human and mouse Ret share 83% amino acid sequence homology (77% homology in the extracellular domain and 93% homology in the cytoplasmic domain). Although Ret does not bind GDNF ligands directly, the extracellular domain of Ret binds the GDNF-GFR-alpha  complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha.

  1. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
  2. Enokido, Y. et al. (1998) Curr. Biol. 8:1019.
  3. Carlomagno, F. et al. (1998) Endocrinology, 139:3613.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ret Antibody (AF482)(22)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 6 applications: ICC, IHC, IHC Frozen, IHC Paraffin-embedded, IP, WB.


Filter By Application
ICC
(9)
IHC
(4)
IHC Frozen
(3)
IHC Paraffin-embedded
(1)
IP
(1)
WB
(4)
All Applications
Filter By Species
Mouse
(18)
Rat
(4)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using AF482 Applications Species
HJ Park, EC Bolton RET-mediated glial cell line derived neurotrophic factor signaling inhibits mouse prostate development Development, 2017;0(0):. 2017 [PMID: 28506996] (WB,, Mouse) WB Mouse
JL Trevaskis, CB Sacramento, H Jouihan, S Ali, J Le Lay, S Oldham, N Bhagroo, BB Boland, J Cann, Y Chang, T O'Day, V Howard, C Reers, MS Winzell, DM Smith, M Feigh, P Barkholt, K Schreiter, M Austen, U Andag, S Thompson, L Jermutus, MP Coghlan, J Grimsby, C Dohrmann, CJ Rhodes, CM Rondinone, A Sharma Neurturin and a Glp-1 Analogue Act Synergistically to Alleviate Diabetes in Zucker Diabetic Fatty Rats Diabetes, 2017;0(0):. 2017 [PMID: 28408435] (IHC,, Rat) IHC Rat
L Urien, S Gaillard, L Lo Re, P Malapert, M Bohic, A Reynders, A Moqrich Genetic ablation of GINIP-expressing primary sensory neurons strongly impairs Formalin-evoked pain Sci Rep, 2017;7(0):43493. 2017 [PMID: 28240741] (IHC Frozen,, Mouse) IHC Frozen Mouse
NA Gabreski, JK Vaghasia, SS Novakova, NQ McDonald, BA Pierchala Exon Skipping in the RET Gene Encodes Novel Isoforms That Differentially Regulate RET Protein Signal Transduction J. Biol. Chem., 2016;291(31):16249-62. 2016 [PMID: 27226544] (WB,, Mouse) WB Mouse
Elziere L, Sar C, Venteo S, Bourane S, Puech S, Sonrier C, Boukhadaoui H, Fichard A, Pattyn A, Valmier J, Carroll P, Mechaly I CaMKK-CaMK1a, a new post-traumatic signalling pathway induced in mouse somatosensory neurons. PLoS ONE, 2014;9(5):e97736. 2014 [PMID: 24840036] (IHC,, Mouse) IHC Mouse
Gorokhova S, Gaillard S, Urien L, Malapert P, Legha W, Baronian G, Desvignes J, Alonso S, Moqrich A Uncoupling of molecular maturation from peripheral target innervation in nociceptors expressing a chimeric TrkA/TrkC receptor. PLoS Genet, 2014;10(2):e1004081. 2014 [PMID: 24516396] (IHC,, Mouse) IHC Mouse
Gatto G, Dudanova I, Suetterlin P, Davies A, Drescher U, Bixby J, Klein R Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling. J Neurosci, 2013;33(12):5399-410. 2013 [PMID: 23516305] (IHC Frozen,, Mouse) IHC Frozen Mouse
Vaegter CB, Jansen P, Fjorback AW Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling. Nat. Neurosci., 2011;14(1):54-61. 2011 [PMID: 21102451] (WB,, Mouse) WB Mouse
Kawaguchi J, Nichols J, Gierl MS, Faial T, Smith A Isolation and propagation of enteric neural crest progenitor cells from mouse embryonic stem cells and embryos. Development, 2010;137(5):693-704. 2010 [PMID: 20147374] (ICC,, Mouse) ICC Mouse
Milenkovic N, Frahm C, Gassmann M, Griffel C, Erdmann B, Birchmeier C, Lewin GR, Garratt AN Nociceptive tuning by stem cell factor/c-Kit signaling. Neuron, 2007;56(5):893-906. 2007 [PMID: 18054864] (ICC,, Mouse) ICC Mouse
Show All 22 Publications.

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Bioinformatics

Gene Symbol RET
Entrez
Uniprot