Western Blot: GFAP Antibody (GA5) [NBP2-29415] - Analysis of GFAP in human brain lysate using GFAP (GA5) antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for ...read more
Immunohistochemistry-Paraffin: GFAP Antibody (GA5) [NBP2-29415] - Formalin-paraffin human brain stained with GFAP Ab (GA-5). Note cytoplasmic staining.
Simple Western: GFAP Antibody (GA5) [NBP2-29415] - Simple Western lane view shows a specific band for GFAP in 0.2 mg/ml of h. Brain lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Simple Western: GFAP Antibody (GA5) [NBP2-29415] - Electropherogram image of the corresponding Simple Western lane. GFAP antibody was used at 10 ug/ml dilution of h. Brain lysates(s) respectively.
Hu, Mu, Rt, Po, Bv, Ch, RbSpecies Glossary
WB, Simple Western, Flow, ICC/IF, IHC-Fr, IHC-P
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Glial fibrillary protein from porcine spinal cord was used as the immunogen for the GFAP (GA5) antibody.
This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Protein A or G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Human Chromosome Location:17q21.31 Immunohistochemistry (cryosections): see Tobin et. al. for details. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP2-29415 in the following applications:
Glial fibrillary acidic protein (GFAP) is a class-III intermediate-filament (IF) protein that is highly specific for cells of astroglial lineage, although its tissue-specific role is speculative. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. The gene is localized to chromosome 17q21. Alternate splicing of this gene generates several transcript variants encoding three different isoforms.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
GFAP: Roles in Alzheimer's and Schizophrenia Glial fibrillary acidic protein (GFAP) is a class III intermediate filament (IF) protein and is used as a marker to distinguish astrocytes from other glial cells during development. GFP may play a role in maintaining mechanical strength and shape in a... Read full blog post.
Vimentin in Wound Healing Vimentin is a fundamental 10 nm type III intermediate filament (IF) protein found in many mesenchymal and epithelia tissues, tissue culture cells, and developing neuronal and astrocytic precursor cells of the central nervous system. It frequently co-p... Read full blog post.
"Whats the Hap" with GFAP? Glial Fibrillary Acidic Protein (GFAP) is one of the major intermediate filament axonal proteins found in mature astrocytes, the star-shaped glial cells that comprise the majority of cells within the central nervous system (1). Astrocytes perform a wi... Read full blog post.