Western Blot: GFAP Antibody (GA5) [NBP2-29415] - Analysis of GFAP in human brain lysate using GFAP (GA5) antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for ...read more
Immunocytochemistry/ Immunofluorescence: GFAP Antibody (GA5) [NBP2-29415] - Cultured Rat Hippocampal Neurons, image courtesy of customer.
Immunohistochemistry-Paraffin: GFAP Antibody (GA5) [NBP2-29415] - Formalin-paraffin human brain stained with GFAP Ab (GA-5). Note cytoplasmic staining.
Simple Western: GFAP Antibody (GA5) [NBP2-29415] - Simple Western lane view shows a specific band for GFAP in 0.2 mg/ml of h. Brain lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Simple Western: GFAP Antibody (GA5) [NBP2-29415] - Electropherogram image of the corresponding Simple Western lane. GFAP antibody was used at 10 ug/ml dilution of h. Brain lysates(s) respectively.
Glial fibrillary protein from porcine spinal cord was used as the immunogen for the GFAP (GA5) antibody.
This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Protein A or G purified
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Human Chromosome Location:17q21.31 Immunohistochemistry (cryosections): see Tobin et. al. for details.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Glial fibrillary acidic protein (GFAP) is a class-III intermediate-filament (IF) protein that is highly specific for cells of astroglial lineage, although its tissue-specific role is speculative. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. The gene is localized to chromosome 17q21. Alternate splicing of this gene generates several transcript variants encoding three different isoforms.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Fixation: 3% formaldehyde (TAAB) in PBS, 15 minutes RT Blocking: 10% donkey serum in PBS, 1 hour RT Permeabilization: 0.2% Triton-X100 Primary Antibody: 1:100 in 5% horse serum in PBS with 0.2% Triton, overnight, 4°C Wash: three times wash with PBS with 0.2% Triton, 10 min each Secondary Antibody: Invitrogen goat anti-mouse Alexa568, 1:500 in PBS with 0.2% Triton, 2 hours, RT Wash: three times wash with PBS, 10 min each Microscope: Confocal (Zeiss LSM710)
The antibody labeld intermediate filaments in cultured astrocytes.
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