GFAP Antibody (GA5)


Western Blot: GFAP Antibody (GA5) [NBP2-29415] - Analysis of GFAP in human brain lysate using GFAP (GA5) antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for more
Immunohistochemistry-Paraffin: GFAP Antibody (GA5) [NBP2-29415] - Formalin-paraffin human brain stained with GFAP Ab (GA-5). Note cytoplasmic staining.
Simple Western: GFAP Antibody (GA5) [NBP2-29415] - Simple Western lane view shows a specific band for GFAP in 0.2 mg/ml of h. Brain lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa more
Simple Western: GFAP Antibody (GA5) [NBP2-29415] - Electropherogram image of the corresponding Simple Western lane. GFAP antibody was used at 10 ug/ml dilution of h. Brain lysates(s) respectively.

Product Details

Reactivity Hu, Mu, Rt, Po, Bv, Ch, RbSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC-Fr, IHC-P
Please see the vial label for concentration. If unlisted please contact technical services.

GFAP Antibody (GA5) Summary

Glial fibrillary protein from porcine spinal cord was used as the immunogen for the GFAP (GA5) antibody.
Astrocyte Marker
This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
10mM PBS and 0.05% BSA
0.05% Sodium Azide
Protein A or G purified


  • Western Blot 0.5-1ug/ml
  • Simple Western 10 ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
Application Notes
Human Chromosome Location:17q21.31
Immunohistochemistry (cryosections): see Tobin et. al. for details. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NBP2-29415 in the following applications:

Alternate Names for GFAP Antibody (GA5)

  • FLJ45472
  • GFAP astrocytes
  • GFAP elisa
  • GFAP flow cytometry
  • glial fibrillary acidic protein


Glial fibrillary acidic protein (GFAP) is a class-III intermediate-filament (IF) protein that is highly specific for cells of astroglial lineage, although its tissue-specific role is speculative. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. The gene is localized to chromosome 17q21. Alternate splicing of this gene generates several transcript variants encoding three different isoforms.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GFAP Antibody (NBP2-29415)(3)

Reviews for GFAP Antibody (NBP2-29415) (0)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GFAP Antibody (NBP2-29415). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in native or recombinant GFAP protein without GST Tag or in urea buffer. Can I use your GFAP antibody (NB100-53809) to detect P0809, P0810 and P1691 GFAP proteins?
    • P0809, P0810 and P1691 are blocking peptides that are not suitable for use with NB100-53809. Unfortunately, we do not carry a GFAP protein without a GST tag. I apologize for the inconvenience.

Secondary Antibodies


Isotype Controls

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Blogs on GFAP.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain. ¬†Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).¬† The importance of t...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.¬† Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

Glial Fibrillary Acidic Protein (GFAP), The Most Popular Astrocyte Marker
GFAP, a class-III intermediate filament, is a 50kDa protein which is found in the mature and developing astrocytes in the CNS, non-myelinating Schwann cells in the PNS, enteric glial cells (enteric nervous system/ENS), ependymal cells, and radial g...  Read full blog post.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

GFAP: Roles in Alzheimer's and Schizophrenia
Glial fibrillary acidic protein (GFAP) is a class III intermediate filament (IF) protein and is used as a marker to distinguish astrocytes from other glial cells during development. GFP may play a role in maintaining mechanical strength and shape in a...  Read full blog post.

Vimentin in Wound Healing
Vimentin is a fundamental 10 nm type III intermediate filament (IF) protein found in many mesenchymal and epithelia tissues, tissue culture cells, and developing neuronal and astrocytic precursor cells of the central nervous system. It frequently co-p...  Read full blog post.

"Whats the Hap" with GFAP?
Glial Fibrillary Acidic Protein (GFAP) is one of the major intermediate filament axonal proteins found in mature astrocytes, the star-shaped glial cells that comprise the majority of cells within the central nervous system (1). Astrocytes perform a wi...  Read full blog post.

GFAP Antibodies are a 'No Brainer' for Neurodegenerative Research
Glial fibrillary acidic protein (GFAP) is the main intermediate filament protein in mature astrocytes, but also an important component of the cytoskeleton in astrocytes during development. Recent developments using GFAP antibodies in astrocyte biology...  Read full blog post.

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Product PDFs


Gene Symbol GFAP

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