GFAP Antibody (273807) [Unconjugated]

Images

 
GFAP was detected in immersion fixed differentiated rat cortical stem cells using Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB2594) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more
Western blot shows lysates of human brain (motor cortex) tissue, human brain (cerebellum) tissue, and human brain (hypothalamus) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GFAP Monoclonal ...read more
Simple Western lane view shows lysates of human brain (cerebellum) tissue and human brain (motor cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for GFAP at approximately 51-52 kDa (as ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clone
273807
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

GFAP Antibody (273807) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GFAP
Leu292-Met432
Accession # P14136
Specificity
Detects human GFAP in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GFAP
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 50 ug/mL
  • Immunocytochemistry 8-25 ug/mL
Control
GFAP Lysate (NBP2-66169)
Reviewed Applications
Read 1 Review rated 5
using
MAB2594 in the following applications:

Publications
Read Publications using
MAB2594 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GFAP Antibody (273807) [Unconjugated]

  • FLJ45472
  • GFAP astrocytes
  • GFAP
  • glial fibrillary acidic protein

Background

Glial Fibrillary Acidic Protein (GFAP) is the predominant component of astrocyte intermediate filaments in the central nervous system. It has also been detected in the glial cells of the enteric nervous system and some Schwann cells in the peripheral nervous systems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GFAP Antibody (MAB2594)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC, IHC, IHC - Not specified, IHC-OCT embedded.


Filter By Application
ICC
(3)
IHC
(1)
IHC - Not specified
(1)
IHC-OCT embedded
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(2)
All Species
Showing Publications 1 - 6 of 6.
Publications using MAB2594 Applications Species
Cruz-Orengo L, Daniels B, Dorsey D, Basak S, Grajales-Reyes J, McCandless E, Piccio L, Schmidt R, Cross A, Crosby S, Klein R Enhanced sphingosine-1-phosphate receptor 2 expression underlies female CNS autoimmunity susceptibility. J Clin Invest, 2014;124(6):2571-84. 2014 [PMID: 24812668] (IHC - Not specified,, Human) IHC - Not specified Human
Zeng, L, He, X, Wang, Y, Tang, Y, Zheng, C, Cai, H, Liu, J, Wang, Y, Fu, Y, Yang, G-Y MicroRNA-210 overexpression induces angiogenesis and neurogenesis in the normal adult mouse brain. Gene Ther, 2014;21(1):37-43. 2014 [PMID: 24152581] (IHC-OCT embedded,, Mouse) IHC-OCT embedded Mouse
Mangiola, Annunzia, Saulnier, Nathalie, De Bonis, Pasquale, Orteschi, Daniela, Sica, Gigliola, Lama, Gina, Pettorini, Benedett, Sabatino, Giovanni, Zollino, Marcella, Lauriola, Libero, Colabianchi, Anna, Proietti, Gabriell, Kovacs, Gyula, Maira, Giulio, Anile, Carmelo Gene expression profile of glioblastoma peritumoral tissue: an ex vivo study. PLoS ONE, 2013;8(3):e57145. 2013 [PMID: 23472076] (IHC,, Human) IHC Human
Walter D, Satheesha S, Albrecht P, Bornhauser BC, D'Alessandro V, Oesch SM, Rehrauer H, Leuschner I, Koscielniak E, Gengler C, Moch H, Bernasconi M, Niggli FK, Schafer BW CD133 positive embryonal rhabdomyosarcoma stem-like cell population is enriched in rhabdospheres. PLoS ONE, 2011;6(5):e19506. 2011 [PMID: 21602936] (ICC,, Human) ICC Human
Thon N, Damianoff K, Hegermann J, Grau S, Krebs B, Schnell O, Tonn JC, Goldbrunner R Presence of pluripotent CD133+ cells correlates with malignancy of gliomas. Mol. Cell. Neurosci., 2010;43(1):51-9. 2010 [PMID: 18761091] (ICC,, Human) ICC Human
Ponomarev ED, Novikova M, Maresz K, Shriver LP, Dittel BN Development of a culture system that supports adult microglial cell proliferation and maintenance in the resting state. J. Immunol. Methods, 2005;300(1):32-46. 2005 [PMID: 15893321] (ICC,, Mouse) ICC Mouse

Review for GFAP Antibody (MAB2594) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB2594:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Human 02/19/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22985741
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22985741

Details

Detection NotesSee PMID 22985741

Comments

CommentsIndependent review from antibodyresource.com

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GFAP Antibody (MAB2594). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in native or recombinant GFAP protein without GST Tag or in urea buffer. Can I use your GFAP antibody (NB100-53809) to detect P0809, P0810 and P1691 GFAP proteins?
    • P0809, P0810 and P1691 are blocking peptides that are not suitable for use with NB100-53809. Unfortunately, we do not carry a GFAP protein without a GST tag. I apologize for the inconvenience.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for GFAP Antibody (MAB2594)

Discover related pathways, diseases and genes to GFAP Antibody (MAB2594). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for GFAP Antibody (MAB2594)

Discover more about diseases related to GFAP Antibody (MAB2594).
 

Pathways for GFAP Antibody (MAB2594)

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PTMs for GFAP Antibody (MAB2594)

Learn more about PTMs related to GFAP Antibody (MAB2594).

Blogs on GFAP.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

Glial Fibrillary Acidic Protein (GFAP), The Most Popular Astrocyte Marker
GFAP, a class-III intermediate filament, is a 50kDa protein which is found in the mature and developing astrocytes in the CNS, non-myelinating Schwann cells in the PNS, enteric glial cells (enteric nervous system/ENS), ependymal cells, and radial g...  Read full blog post.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

GFAP: Roles in Alzheimer's and Schizophrenia
Glial fibrillary acidic protein (GFAP) is a class III intermediate filament (IF) protein and is used as a marker to distinguish astrocytes from other glial cells during development. GFP may play a role in maintaining mechanical strength and shape in a...  Read full blog post.

Vimentin in Wound Healing
Vimentin is a fundamental 10 nm type III intermediate filament (IF) protein found in many mesenchymal and epithelia tissues, tissue culture cells, and developing neuronal and astrocytic precursor cells of the central nervous system. It frequently co-p...  Read full blog post.

"Whats the Hap" with GFAP?
Glial Fibrillary Acidic Protein (GFAP) is one of the major intermediate filament axonal proteins found in mature astrocytes, the star-shaped glial cells that comprise the majority of cells within the central nervous system (1). Astrocytes perform a wi...  Read full blog post.

GFAP Antibodies are a 'No Brainer' for Neurodegenerative Research
Glial fibrillary acidic protein (GFAP) is the main intermediate filament protein in mature astrocytes, but also an important component of the cytoskeleton in astrocytes during development. Recent developments using GFAP antibodies in astrocyte biology...  Read full blog post.

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Recent Reviews

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Anonymous
02/19/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol GFAP
Entrez
Uniprot