Galectin-9 Antibody [Biotin] - Short Isoform

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Galectin-9 Antibody [Biotin] - Short Isoform Summary

• Immunogen: E. coli-derived recombinant mouse Galectin‑9 short isoform
  Ala2-Thr353; aa148-178 deleted
  Accession # O08573
• Specificity: Detects mouse Galectin-9 in Western blots. In this format, less than 1% cross-reactivity with recombinant human (rh) Galectin‑2, rhGalectin‑8, recombinant mouse (rm) Galectin-1, rmGalectin-3, rmGalectin-4, and rmGalectin-7 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LGALS9
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-9 Antibody [Biotin] - Short Isoform

• Ecalectin
• GAL9
• gal-9
• galectin 9
• Galectin9
• Galectin-9
• HUAT
• lectin, galactoside-binding, soluble, 9
• LGALS9
• LGALS9A
• MGC117375
• MGC125973
• MGC125974
• Tumor antigen HOM-HD-21
• urate transporter/channel protein

Background

Galectin-9, also known as Ecalectin, belongs to the Galectin family (S-type lectins) of galactoside-binding proteins. It is a 36 - 39 kDa protein that lacks a secretion signal sequence, but can be secreted by a non-classical pathway. Galectin-9 is a tandem-repeat Galectin with two Galectin domains joined by a linker region. Two alternative spliced variants, a short and a long isoform, exist. Galectin-9 short isoform is expressed in a variety of tissues and cells, including fibroblasts, hepatocytes, endothelial cells and astrocytes. The long isoform, however, is expressed primarily in the intestine. Galectin-9 binds TIM-3 on Th1 cells, inducing apoptosis. Mouse Galectin-9 shares 69% and 85% amino acid sequence identity with human and rat Galectin-9, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Galectin-9 Antibody (BAF3535)(2)

Publications using BAF3535

• CC Liang, CS Li, IC Weng, HY Chen, HH Lu, CC Huang, FT Liu Galectin-9 is Critical for Mucosal Adaptive Immunity through the TH17-IgA Axis Am. J. Pathol., 2018-02-17;0(0):. 2018-02-17 [PMID: 29458010] (ELISA Detection, Mouse)
• Reddy PB, Sehrawat S, Suryawanshi A Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency. J. Immunol., 2011-10-21;187(11):5745-55. 2011-10-21 [PMID: 22021615] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: LGALS9
• Uniprot:
  • O08573()