Galectin-9 Antibody [Alexa Fluor® 647]

• Reactivity: Mu
• Applications: WB, CyTOF-ready, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

Galectin-9 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant mouse Galectin‑9
  Ala2-Thr353; aa 148-178 deletion
  Accession # O08573
• Specificity: Detects mouse Galectin‑9 in direct ELISAs and Western blots. In Western blots, approximately 30% cross-reactivity with recombinant human (rh) Galectin-9 is observed. In direct ELISAs and Western blots, less than 2% cross‑reactivity with rhGalect
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry
  • Intracellular Staining by Flow Cytometry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-9 Antibody [Alexa Fluor® 647]

• Ecalectin
• GAL9
• gal-9
• galectin 9
• Galectin9
• Galectin-9
• HUAT
• lectin, galactoside-binding, soluble, 9
• LGALS9
• LGALS9A
• MGC117375
• MGC125973
• MGC125974
• Tumor antigen HOM-HD-21
• urate transporter/channel protein

Background

Galectin-9, also known as Ecalectin, belongs to the Galectin family (S-type lectins) of galactoside-binding proteins. It is a 36‑39 kDa protein that lacks a secretion signal sequence, but can be secreted by a non-classical pathway. Galectin-9 is a tandem-repeat Galectin with two Galectin domains joined by a linker region. Two alternative spliced variants, a short and a long isoform, exist. Galectin-9 short isoform is expressed in a variety of tissues and cells, including fibroblasts, hepatocytes, endothelial cells and astrocytes. The long isoform, however, is expressed primarily in the intestine. Galectin-9 binds TIM-3 on Th1 cells, inducing apoptosis. Mouse Galectin-9 shares 69% and 85% amino acid sequence identity with human and rat Galectin-9, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.