Galectin-9 Antibody [Unconjugated]

Mouse thymocytes were stained with Goat Anti-Mouse Galectin-9 Short Isoform Affinity-purified Polyclonal Antibody (Catalog # AF3535, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Galectin-9 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Galectin-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3535) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: WB, CyTOF-ready, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Galectin-9 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Galectin‑9
  Ala2-Thr353; aa 148-178 deletion
  Accession # O08573
• Specificity: Detects mouse Galectin‑9 in direct ELISAs and Western blots. In Western blots, approximately 30% cross-reactivity with recombinant human (rh) Galectin-9 is observed. In direct ELISAs and Western blots, less than 2% cross‑reactivity with rhGalectin-1, rhGalectin-2, rhGalectin-3, rhGalectin-7, rhGalectin-8, recombinant mouse (rm) Galectin-1, rmGalectin-3, rmGalectin-4, and rmGalectin-7 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LGALS9
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-9 Antibody [Unconjugated]

• Ecalectin
• GAL9
• gal-9
• galectin 9
• Galectin9
• Galectin-9
• HUAT
• lectin, galactoside-binding, soluble, 9
• LGALS9
• LGALS9A
• MGC117375
• MGC125973
• MGC125974
• Tumor antigen HOM-HD-21
• urate transporter/channel protein

Background

Galectin-9, also known as Ecalectin, belongs to the Galectin family (S-type lectins) of galactoside-binding proteins. It is a 36‑39 kDa protein that lacks a secretion signal sequence, but can be secreted by a non-classical pathway. Galectin-9 is a tandem-repeat Galectin with two Galectin domains joined by a linker region. Two alternative spliced variants, a short and a long isoform, exist. Galectin-9 short isoform is expressed in a variety of tissues and cells, including fibroblasts, hepatocytes, endothelial cells and astrocytes. The long isoform, however, is expressed primarily in the intestine. Galectin-9 binds TIM-3 on Th1 cells, inducing apoptosis. Mouse Galectin-9 shares 69% and 85% amino acid sequence identity with human and rat Galectin-9, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Galectin-9 Antibody (AF3535)(8)

Publications using AF3535

• Richard Jennemann, Sylvia Kaden, Martina Volz, Viola Nordström, Silke Herzer, Roger Sandhoff et al. Gangliosides modulate insulin secretion by pancreatic beta cells under glucose stress Glycobiology 2020-08-20 [PMID: 32149357]
• Laabiah Wasim, Fathima Hifza Mohamed Buhari, Myuran Yoganathan, Taylor Sicard, June Ereño-Orbea, Jean-Philippe Julien et al. N-Linked Glycosylation Regulates CD22 Organization and Function Frontiers in Immunology 2019-04-04 [PMID: 31019513] (Flow Cytometry, Mouse)
• C Lhuillier, C Barjon, V Baloche, T Niki, A Gelin, R Mustapha, L Claër, S Hoos, Y Chiba, M Ueno, M Hirashima, M Wei, O Morales, B Raynal, N Delhem, O Dellis, P Busson Characterization of neutralizing antibodies reacting with the 213-224 amino-acid segment of human galectin-9 PLoS ONE, 2018-09-11;13(9):e0202512. 2018-09-11 [PMID: 30204750] (Western Blot, Mouse)
• CC Liang, CS Li, IC Weng, HY Chen, HH Lu, CC Huang, FT Liu Galectin-9 is Critical for Mucosal Adaptive Immunity through the TH17-IgA Axis Am. J. Pathol., 2018-02-17;0(0):. 2018-02-17 [PMID: 29458010] (ELISA Capture, Mouse)
• Steelman , Andrew J, Li , Jianrong Astrocyte galectin-9 potentiates microglial TNF secretion. J Neuroinflammation, 2014-08-27;11(0):144. 2014-08-27 [PMID: 25158758] (Western Blot, Rat)
• Steelman A, Smith R, Welsh C, Li J Galectin-9 protein is up-regulated in astrocytes by tumor necrosis factor and promotes encephalitogenic T-cell apoptosis. J Biol Chem, 2013-07-08;288(33):23776-87. 2013-07-08 [PMID: 23836896] (Western Blot, Mouse)
• Leitner , Judith, Rieger , Armin, Pickl , Winfried, Zlabinger , Gerhard, Grabmeier-Pfistershammer , Katharin, Steinberger , Peter TIM-3 does not act as a receptor for galectin-9. PLoS Pathog, 2013-03-21;9(3):e1003253. 2013-03-21 [PMID: 23555261] (Flow Cytometry, Mouse)
• Iqbal AJ, Sampaio AL, Maione F, Greco KV, Niki T, Hirashima M, Perretti M, Cooper D Endogenous galectin-1 and acute inflammation: emerging notion of a galectin-9 pro-resolving effect. Am. J. Pathol., 2011-03-01;178(3):1201-9. 2011-03-01 [PMID: 21356371] (IHC-P, Western Blot, Mouse)

Reviews for Galectin-9 Antibody (AF3535) (3)

Average Rating: 4

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 01/07/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23836896
• Species: Mouse

reviewed by: Verified Customer WB Mouse 01/07/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 19851072
• Species: Mouse

reviewed by: Verified Customer WB Mouse 01/07/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 19670381
• Species: Mouse

Bioinformatics

• Gene Symbol: LGALS9
• Uniprot:
  • O08573()