Mouse CCL11/Eotaxin Quantikine ELISA Kit, 2 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse CCL11/Eotaxin Quantikine ELISA Kit, 2 Plate Summary

• Background: The Quantikine Mouse Eotaxin Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Eotaxin in cell culture supernates, serum, and plasma. It contains recombinant mouse Eotaxin and antibodies raised against the recombinant protein. This immunoassay has been shown to quantitate the recombinant protein accurately. Results obtained using natural mouse Eotaxin showed dose curves t...hat were parallel to the standard curves obtained using the E. coli-expressed kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Eotaxin.
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• Specificity: Natural and recombinant mouse Eotaxin. This assay cross-reacts 0.062% with Human Eotaxin and recognizes Rat Eotaxin.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Ccl11

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse CCL11/Eotaxin Quantikine ELISA Kit, 2 Plate

• C-C motif chemokine 11
• CCL11
• chemokine (C-C motif) ligand 11
• Eosinophil chemotactic protein
• Eotaxin
• eotaxin-1
• MGC22554
• SCYA11Small-inducible cytokine A11
• small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

Background

CCL11/Eotaxin is a chemokine that interacts with CCR3 to induce eosinophil, basophil, dendritic cell, and Th2 cell chemoattraction during inflammatory and allergic reactions. Its production is upregulated in airway epithelium, alveolar macrophages, eosinophils, smooth muscle cells, chondrocytes, and fibroblasts following allergen or inflammatory challenge. CCL11 is also involved in the growth of myeloid cell progenitors and the differentiation of mast cells.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CCL11/Eotaxin (MME00)(58)

Publications using MME00

• G Scabia, G Testa, M Scali, S Del Turco, G Desiato, N Berardi, A Sale, M Matteoli, L Maffei, M Maffei, M Mainardi, “Train the Reduced ccl11/eotaxin mediates the beneficial effects of environmental stimulation on the aged hippocampus Brain, Behavior, and Immunity, 2021-08-19;98(0):234-244. 2021-08-19 [PMID: 34418501] (Mouse)
• W Gao, Z Jin, Y Zheng, Y Xu Psoralen inhibits the inflammatory response and mucus production in allergic rhinitis by inhibiting the activator protein�1 pathway and the downstream expression of cystatin?SN Molecular Medicine Reports, 2021-07-19;24(3):. 2021-07-19 [PMID: 34278468] (Human)
• SJ Wu, CJ Liou, YL Chen, SC Cheng, WC Huang Fucoxanthin Ameliorates Oxidative Stress and Airway Inflammation in Tracheal Epithelial Cells and Asthmatic Mice Cells, 2021-05-25;10(6):. 2021-05-25 [PMID: 34070405] (Mouse)
• ME Kotas, J Dion, S Van Dyken, RR Ricardo-Go, CJ Danel, C Taillé, L Mouthon, RM Locksley, B Terrier A role for IL-33-activated ILC2s in eosinophilic vasculitis JCI Insight, 2021-06-22;0(0):. 2021-06-22 [PMID: 33974563] (Mouse)
• S Piñeiro-He, P Martínez, MA Blasco Short and dysfunctional telomeres protect from allergen-induced airway inflammation Aging Cell, 2021-05-04;20(5):e13352. 2021-05-04 [PMID: 33942458] (Mouse)
• R Wang, K Huang CCL11 increases the proportion of CD4+CD25+Foxp3+ Treg cells and the production of IL?2 and TGF?&beta by CD4+ T�cells via the STAT5 signaling pathway Mol Med Rep, 2020-04-01;0(0):. 2020-04-01 [PMID: 32323817] (Mouse)
• HS Choi, T Won, X Hou, G Chen, W Bracamonte, MV Talor, I Jur?ová, O Szárszoi, L ?urnova, I St?íž, JE Hooper, V Melenovský, D ?iháková Innate Lymphoid Cells Play a Pathogenic Role in Pericarditis Cell Rep, 2020-03-03;30(9):2989-3003.e6. 2020-03-03 [PMID: 32130902] (Mouse)
• Brittany Woods, Wei Chen, Sophia Chiu, Christian Marinaccio, Chunling Fu, Lilly Gu et al. Activation of JAK/STAT Signaling in Megakaryocytes Sustains Myeloproliferation In Vivo Clinical Cancer Research 2019-10-01 [PMID: 31217200] (Transgenic Mouse)
• I Sulaiman, K Tan, N Mohtarrudi, JC Woei Lim, J Stanslas Andrographolide prevented toluene diisocyanate-induced occupational asthma and aberrant airway E-cadherin distribution via p38 MAPK-dependent Nrf2 induction Pulm Pharmacol Ther, 2018-09-21;0(0):. 2018-09-21 [PMID: 30244166] (Mouse)
• A Aguilar-Va, N Haji, D De Gregori, E Matta-Cama, MJ Eslamizade, J Popic, V Sharma, R Cao, C Rummel, A Tanti, S Wiebe, N Nuñez, S Comai, R Nadon, G Luheshi, N Mechawar, G Turecki, JC Lacaille, G Gobbi, N Sonenberg Translational control of depression-like behavior via phosphorylation of eukaryotic translation initiation factor 4E Nat Commun, 2018-06-25;9(1):2459. 2018-06-25 [PMID: 29941989] (Mouse)
• Macrophages and cardiac fibroblasts are the main producers of eotaxins and regulate eosinophil trafficking to the heart Eur J Immunol, 2016-10-25;0(0):. 2016-10-25 [PMID: 27621211] (Mouse)
• Increased mitochondrial arginine metabolism supports bioenergetics in asthma J Clin Invest, 2016-05-23;0(0):. 2016-05-23 [PMID: 27214549] (Mouse)
• Itoga M, Konno Y, Moritoki Y, Saito Y, Ito W, Tamaki M, Kobayashi Y, Kayaba H, Kikuchi Y, Chihara J, Takeda M, Ueki S, Hirokawa M G-protein-coupled estrogen receptor agonist suppresses airway inflammation in a mouse model of asthma through IL-10. PLoS ONE, 2015-03-31;10(3):e0123210. 2015-03-31 [PMID: 25826377] (Mouse)
• Medina , Jorge L, Coalson , Jacqueli, Brooks , Edward G, Le Saux , Claude J, Winter , Vicki T, Chaparro , Adriana, Principe , Molly F, Solis , Laura, Kannan , T R, Baseman , Joel B, Dube , Peter H Mycoplasma pneumoniae CARDS toxin exacerbates ovalbumin-induced asthma-like inflammation in BALB/c mice. PLoS ONE, 2014-07-24;9(7):e102613. 2014-07-24 [PMID: 25058417] (Mouse)
• Erickson , Michelle, Morofuji , Yoichi, Owen , Joshua B, Banks , William Rapid transport of CCL11 across the blood-brain barrier: regional variation and importance of blood cells. J Pharmacol Exp Ther, 2014-04-04;349(3):497-507. 2014-04-04 [PMID: 24706984] (Mouse)
• Johnson T, Rangel D, Graham B, Brough D, Gall J Genetic vaccine for respiratory syncytial virus provides protection without disease potentiation. Mol Ther, 2013-06-10;22(1):196-205. 2013-06-10 [PMID: 23752342] (Mouse)
• Cho SH, Oh SY, Zhu Z, Lee J, Lane AP Spontaneous eosinophilic nasal inflammation in a genetically-mutant mouse: comparative study with an allergic inflammation model. PLoS ONE, 2012-04-11;7(4):e35114. 2012-04-11 [PMID: 22509389] (Mouse)
• Barlow JL, Flynn RJ, Ballantyne SJ, McKenzie AN Reciprocal expression of IL-25 and IL-17A is important for allergic airways hyperreactivity. Clin. Exp. Allergy, 2011-07-04;41(10):1447-55. 2011-07-04 [PMID: 21722219] (Mouse)
• Yamamoto Y, Otani S, Hirai H, Nagata K, Aritake K, Urade Y, Narumiya S, Yokozeki H, Nakamura M, Satoh T Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2. Am. J. Pathol., 2011-05-10;179(1):302-14. 2011-05-10 [PMID: 21703412] (Mouse)
• Besnard AG, Togbe D, Guillou N, Erard F, Quesniaux V, Ryffel B IL-33-activated dendritic cells are critical for allergic airway inflammation. Eur. J. Immunol., 2011-05-25;41(6):1675-86. 2011-05-25 [PMID: 21469105] (Mouse)
• Louten J, Rankin AL, Li Y Endogenous IL-33 enhances Th2 cytokine production and T-cell responses during allergic airway inflammation. Int. Immunol., 2011-03-21;23(5):307-15. 2011-03-21 [PMID: 21422152] (Mouse)
• Zhu M, Hug C, Kasahara DI Impact of adiponectin deficiency on pulmonary responses to acute ozone exposure in mice. Am. J. Respir. Cell Mol. Biol., 2009-11-13;43(4):487-97. 2009-11-13 [PMID: 19915153] (Mouse)
• De Alba J, Raemdonck K, Dekkak A, Collins M, Wong S, Nials AT, Knowles RG, Belvisi MG, Birrell MA House dust mite induces direct airway inflammation in vivo: implications for future disease therapy? Eur. Respir. J., 2009-10-19;35(6):1377-87. 2009-10-19 [PMID: 19840954] (Rat)
• Gueders MM, Paulissen G, Crahay C, Quesada-Calvo F, Hacha J, Van Hove C, Tournoy K, Louis R, Foidart JM, Noel A, Cataldo DD Mouse models of asthma: a comparison between C57BL/6 and BALB/c strains regarding bronchial responsiveness, inflammation, and cytokine production. Inflamm. Res., 2009-06-09;58(12):845-54. 2009-06-09 [PMID: 19506803] (Mouse)
• Johnson TR, Rao S, Seder RA, Chen M, Graham BS TLR9 agonist, but not TLR7/8, functions as an adjuvant to diminish FI-RSV vaccine-enhanced disease, while either agonist used as therapy during primary RSV infection increases disease severity. Vaccine, 2009-04-03;27(23):3045-52. 2009-04-03 [PMID: 19428918] (Mouse)
• Shinagawa K, Ploplis VA, Castellino FJ A severe deficiency of coagulation factor VIIa results in attenuation of the asthmatic response in mice. Am. J. Physiol. Lung Cell Mol. Physiol., 2009-03-13;296(5):L763-70. 2009-03-13 [PMID: 19286924] (Mouse)
• Claudio E, Sonder SU, Saret S, Carvalho G, Ramalingam TR, Wynn TA, Chariot A, Garcia-Perganeda A, Leonardi A, Paun A, Chen A, Ren NY, Wang H, Siebenlist U The adaptor protein CIKS/Act1 is essential for IL-25-mediated allergic airway inflammation. J. Immunol., 2009-02-01;182(3):1617-30. 2009-02-01 [PMID: 19155511] (Mouse)
• Miyazaki D, Nakamura T, Ohbayashi M, Kuo CH, Komatsu N, Yakura K, Tominaga T, Inoue Y, Higashi H, Murata M, Takeda S, Fukushima A, Liu FT, Rothenberg ME, Ono SJ Ablation of type I hypersensitivity in experimental allergic conjunctivitis by eotaxin-1/CCR3 blockade. Int. Immunol., 2009-01-15;21(2):187-201. 2009-01-15 [PMID: 19147836] (Mouse)
• Medoff BD, Seung E, Hong S, Thomas SY, Sandall BP, Duffield JS, Kuperman DA, Erle DJ, Luster AD CD11b+ myeloid cells are the key mediators of Th2 cell homing into the airway in allergic inflammation. J. Immunol., 2009-01-01;182(1):623-35. 2009-01-01 [PMID: 19109196] (Mouse)
• Ryman-Rasmussen JP, Tewksbury EW, Moss OR, Cesta MF, Wong BA, Bonner JC Inhaled multiwalled carbon nanotubes potentiate airway fibrosis in murine allergic asthma. Am. J. Respir. Cell Mol. Biol., 2008-09-11;40(3):349-58. 2008-09-11 [PMID: 18787175] (Mouse)
• Ben S, Li X, Xu F, Xu W, Li W, Wu Z, Huang H, Shi H, Shen H Treatment with anti-CC chemokine receptor 3 monoclonal antibody or dexamethasone inhibits the migration and differentiation of bone marrow CD34 progenitor cells in an allergic mouse model. Allergy, 2008-09-01;63(9):1164-76. 2008-09-01 [PMID: 18699933] (Mouse)
• Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection. J. Exp. Med., 2008-08-11;205(9):2075-84. 2008-08-11 [PMID: 18695004] (Mouse)
• Lam D, Ng N, Lee S, Batzer G, Horner AA Airway house dust extract exposures modify allergen-induced airway hypersensitivity responses by TLR4-dependent and independent pathways. J. Immunol., 2008-08-15;181(4):2925-32. 2008-08-15 [PMID: 18684984] (Mouse)
• Johnson TR, Rothenberg ME, Graham BS Pulmonary eosinophilia requires interleukin-5, eotaxin-1, and CD4+ T cells in mice immunized with respiratory syncytial virus G glycoprotein. J. Leukoc. Biol., 2008-06-02;84(3):748-59. 2008-06-02 [PMID: 18519743] (Mouse)
• O'Brien LM, Fitzpatrick E, Baird AW, Campion DP Eosinophil-nerve interactions and neuronal plasticity in rat gut associated lymphoid tissue (GALT) in response to enteric parasitism. J. Neuroimmunol., 2008-05-20;197(1):1-9. 2008-05-20 [PMID: 18495257] (Rat)
• Munitz A, Brandt EB, Mingler M, Finkelman FD, Rothenberg ME Distinct roles for IL-13 and IL-4 via IL-13 receptor alpha1 and the type II IL-4 receptor in asthma pathogenesis. Proc. Natl. Acad. Sci. U.S.A., 2008-05-14;105(20):7240-5. 2008-05-14 [PMID: 18480254] (Mouse)
• Voskas D, Babichev Y, Ling LS, Alami J, Shaked Y, Kerbel RS, Ciruna B, Dumont DJ An eosinophil immune response characterizes the inflammatory skin disease observed in Tie-2 transgenic mice. J. Leukoc. Biol., 2008-05-01;84(1):59-67. 2008-05-01 [PMID: 18443190] (Mouse)
• Cho YS, Oh SY, Zhu Z Tyrosine phosphatase SHP-1 in oxidative stress and development of allergic airway inflammation. Am. J. Respir. Cell Mol. Biol., 2008-04-25;39(4):412-9. 2008-04-25 [PMID: 18441283] (Mouse)
• Therien AG, Bernier V, Weicker S, Tawa P, Falgueyret JP, Mathieu MC, Honsberger J, Pomerleau V, Robichaud A, Stocco R, Dufresne L, Houshyar H, Lafleur J, Ramachandran C, O'Neill GP, Slipetz D, Tan CM Adenovirus IL-13-induced airway disease in mice: a corticosteroid-resistant model of severe asthma. Am. J. Respir. Cell Mol. Biol., 2008-02-07;39(1):26-35. 2008-02-07 [PMID: 18258919] (Mouse)
• Kudlacz E, Conklyn M, Andresen C, Whitney-Pickett C, Changelian P The JAK-3 inhibitor CP-690550 is a potent anti-inflammatory agent in a murine model of pulmonary eosinophilia. Eur. J. Pharmacol., 2007-12-28;582(1):154-61. 2007-12-28 [PMID: 18242596] (Mouse)
• Zheng T, Liu W, Oh SY, Zhu Z, Hu B, Homer RJ, Cohn L, Grusby MJ, Elias JA IL-13 receptor alpha2 selectively inhibits IL-13-induced responses in the murine lung. J. Immunol., 2008-01-01;180(1):522-9. 2008-01-01 [PMID: 18097054] (Mouse)
• Maret M, Ruffie C, Periquet B, Campo AM, Menevret M, Phelep A, Dziewiszek K, Druilhe A, Pretolani M Liposomal retinoic acids modulate asthma manifestations in mice. J. Nutr., 2007-12-01;137(12):2730-6. 2007-12-01 [PMID: 18029491] (Mouse)
• Hiromura Y, Kishida T, Nakano H, Hama T, Imanishi J, Hisa Y, Mazda O IL-21 administration into the nostril alleviates murine allergic rhinitis. J. Immunol., 2007-11-15;179(10):7157-65. 2007-11-15 [PMID: 17982108] (Mouse)
• Esnault S, Rosenthal LA, Shen ZJ, Sedgwick JB, Szakaly RJ, Sorkness RL, Malter JS A critical role for Pin1 in allergic pulmonary eosinophilia in rats. J. Allergy Clin. Immunol., 2007-08-27;120(5):1082-8. 2007-08-27 [PMID: 17720236] (Rat)
• Simeone-Penney MC, Severgnini M, Tu P, Homer RJ, Mariani TJ, Cohn L, Simon AR Airway epithelial STAT3 is required for allergic inflammation in a murine model of asthma. J. Immunol., 2007-05-15;178(10):6191-9. 2007-05-15 [PMID: 17475846] (Mouse)
• Martinez de la Torre Y, Buracchi C, Borroni EM, Dupor J, Bonecchi R, Nebuloni M, Pasqualini F, Doni A, Lauri E, Agostinis C, Bulla R, Cook DN, Haribabu B, Meroni P, Rukavina D, Vago L, Tedesco F, Vecchi A, Lira SA, Locati M, Mantovani A Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6. Proc. Natl. Acad. Sci. U.S.A., 2007-02-05;104(7):2319-24. 2007-02-05 [PMID: 17283337] (Mouse)
• Oh SY, Zheng T, Bailey ML, Barber DL, Schroeder JT, Kim YK, Zhu Z Src homology 2 domain-containing inositol 5-phosphatase 1 deficiency leads to a spontaneous allergic inflammation in the murine lung. J. Allergy Clin. Immunol., 2006-10-23;119(1):123-31. 2006-10-23 [PMID: 17208593] (Mouse)
• Kitamura K, Takeda K, Koya T, Miyahara N, Kodama T, Dakhama A, Takai T, Hirano A, Tanimoto M, Harada M, Gelfand EW Critical role of the Fc receptor gamma-chain on APCs in the development of allergen-induced airway hyperresponsiveness and inflammation. J. Immunol., 2007-01-01;178(1):480-8. 2007-01-01 [PMID: 17182587] (Mouse)
• Lee JS, Lee CM, Jeong YI, Jung ID, Kim BH, Seong EY, Kim JI, Choi IW, Chung HY, Park YM D-pinitol regulates Th1/Th2 balance via suppressing Th2 immune response in ovalbumin-induced asthma. FEBS Lett., 2006-12-11;581(1):57-64. 2006-12-11 [PMID: 17174308] (Mouse)
• Fulkerson PC, Fischetti CA, Hassman LM, Nikolaidis NM, Rothenberg ME Persistent effects induced by IL-13 in the lung. Am. J. Respir. Cell Mol. Biol., 2006-04-27;35(3):337-46. 2006-04-27 [PMID: 16645178] (Mouse)
• Shibamori M, Ogino K, Kambayashi Y, Ishiyama H Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice. Life Sci., 2005-10-17;78(9):987-94. 2005-10-17 [PMID: 16229861] (Mouse)
• Oumouna M, Mapletoft JW, Karvonen BC, Babiuk LA Formulation with CpG oligodeoxynucleotides prevents induction of pulmonary immunopathology following priming with formalin-inactivated or commercial killed bovine respiratory syncytial virus vaccine. J. Virol., 2005-02-01;79(4):2024-32. 2005-02-01 [PMID: 15681404] (Mouse)
• Johnson TR, Varga SM, Braciale TJ, Graham BS Vbeta14(+) T cells mediate the vaccine-enhanced disease induced by immunization with respiratory syncytial virus (RSV) G glycoprotein but not with formalin-inactivated RSV. J. Virol., 2004-08-01;78(16):8753-60. 2004-08-01 [PMID: 15280483] (Mouse)
• Chang EE, Chung LY, Yen CM Kinetics of change in the eotaxin concentration in serum and cerebrospinal fluid of mice infected with Angiostrongylus cantonensis. Parasitol. Res., 2003-11-27;92(2):137-41. 2003-11-27 [PMID: 14648203] (Mouse)
• O'Byrne PM The effects of intranasal budesonide on allergen-induced production of interleukin-5 and eotaxin, airways, blood, and bone marrow eosinophilia, and eosinophil progenitor expansion in sensitized mice. Am. J. Respir. Crit. Care Med., 2002-07-15;166(2):146-53. 2002-07-15 [PMID: 12119225] (Mouse)
• Hanisch UK, Prinz M, Angstwurm K, Hausler KG, Kann O, Kettenmann H, Weber JR The protein tyrosine kinase inhibitor AG126 prevents the massive microglial cytokine induction by pneumococcal cell walls. Eur. J. Immunol., 2001-07-01;31(7):2104-15. 2001-07-01 [PMID: 11449364] (Mouse)
• Guo RF, Lentsch AB, Warner RL, Huber-Lang M, Sarma JV, Hlaing T, Shi MM, Lukacs NW, Ward PA Regulatory effects of eotaxin on acute lung inflammatory injury. J. Immunol., 2001-04-15;166(8):5208-18. 2001-04-15 [PMID: 11290805] (Mouse)
• Knott PG, Gater PR, Dunford PJ, Fuentes ME, Bertrand CP Rapid up-regulation of CXC chemokines in the airways after Ag-specific CD4+ T cell activation. J. Immunol., 2001-01-15;166(2):1233-40. 2001-01-15 [PMID: 11145706] (Mouse)

FAQs for CCL11/Eotaxin (MME00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Ccl11