Galectin-1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Galectin‑1 Antigen ...read more
Galectin‑1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 1.7 µg/mL Goat Anti-Human Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) ...read more
Galectin‑1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) at 5 µg/mL ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑1 at approximately ...read more
Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Normal Goat IgG Control (Catalog # AB-108-C) or (B) Goat Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, CyTOF-ready, ICFlow
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

Galectin-1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Galectin‑1
Ala2-Asp135
Accession # P09382
Specificity
Detects human Galectin-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse (rm) Galectin-1 is observed and less than 5% cross-reactivity with recombinant human (rh) Galectin‑2, rhGalectin‑3, rhGalectin-4, rhGalectin-7, rhGalectin-8, and rmGalectin-9 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LGALS1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Simple Western 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 3 Reviews rated 4.7
using
AF1152 in the following applications:

Publications
Read Publications using
AF1152 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-1 Antibody [Unconjugated]

  • 14 kDa laminin-binding protein
  • 14 kDa lectin
  • Beta-galactoside-binding lectin L-14-I
  • BHL
  • DKFZp686E23103
  • GAL1
  • gal-1
  • Galaptin
  • galectin 1
  • Galectin1
  • Galectin-1
  • GBP
  • HBL
  • HLBP14
  • HPL
  • L-14
  • Lactose-binding lectin 1
  • Lectin galactoside-binding soluble 1
  • lectin, galactoside-binding, soluble, 1
  • LGALS1
  • Putative MAPK-activating protein PM12
  • S-Lac lectin 1

Background

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins.

Galectin-1, also known as L-14, BHL and galaptin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, liver, prostate, lymph nodes, spleen, thymus, placenta, testis, retina, macrophages, B cells, T cells, dendritic cells, and tumor cells. It preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3, and ganglioside GM1. Galectin-1 modulates cell growth and proliferation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting pro-inflammatory cytokine production. Galectin-1 can also modulate cell-cell as well as cell-matrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and anti-inflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity. Human and mouse galectin-1 share about 88% amino acid sequence similarity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow

Publications for Galectin-1 Antibody (AF1152)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: ELISA Capture, Flow, IHC, IHC Paraffin-embedded, WB.


Filter By Application
ELISA Capture
(1)
Flow
(2)
IHC
(1)
IHC Paraffin-embedded
(2)
WB
(2)
All Applications
Filter By Species
Human
(8)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF1152 Applications Species
H Schulz, E Schmoeckel, C Kuhn, S Hofmann, D Mayr, S Mahner, U Jeschke Galectins-1, -3, and -7 Are Prognostic Markers for Survival of Ovarian Cancer Patients Int J Mol Sci, 2017;18(6):. 2017 [PMID: 28594391] (IHC,, Human) IHC Human
MN Andersen, M Ludvigsen, N Abildgaard, I Petruskevi, R Hjortebjer, M Bjerre, B Honoré, HJ Møller, NF Andersen Serum galectin-1 in patients with multiple myeloma: associations with survival, angiogenesis, and biomarkers of macrophage activation Onco Targets Ther, 2017;10(0):1977-1982. 2017 [PMID: 28435287] (ELISA Capture,, Human) ELISA Capture Human
Prototype and Chimera-Type Galectins in Placentas with Spontaneous and Recurrent Miscarriages Int J Mol Sci, 2016;17(5):. 2016 [PMID: 27136536] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Leskela S, Serrano A, de la Fuente H, Rodriguez-Munoz A, Ramos-Levi A, Sampedro-Nunez M, Sanchez-Madrid F, Gonzalez-Amaro R, Marazuela M Graves' disease is associated with a defective expression of the immune regulatory molecule galectin-9 in antigen-presenting dendritic cells. PLoS ONE, 2015;10(4):e0123938. 2015 [PMID: 25880730] (Flow,, Human) Flow Human
Yazawa E, Geddes-Sweeney J, Cedeno-Laurent F, Walley K, Barthel S, Opperman M, Liang J, Lin J, Schatton T, Laga A, Mihm M, Qureshi A, Widlund H, Murphy G, Dimitroff C Melanoma Cell Galectin-1 Ligands Functionally Correlate with Malignant Potential. J Invest Dermatol, 2015;135(7):1849-62. 2015 [PMID: 25756799] (Flow,, Human) Flow Human
Ryu K, Kim S, Kim Y, Woo S, Sung S, Kim H, Jung S, Jo I, Park J Tonsil-derived mesenchymal stem cells alleviate concanavalin A-induced acute liver injury. Exp Cell Res, 2014;326(1):143-54. 2014 [PMID: 24954408] (WB,, Human) WB Human
Suzuki O, Hirsch B, Abe M, Durkop H, Stein H Galectin-1-mediated cell death is increased by CD30-induced signaling in anaplastic large cell lymphoma cells but not in Hodgkin lymphoma cells. Lab. Invest., 2012;92(2):191-9. 2012 [PMID: 21986812] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Zhuo Y, Chammas R, Bellis SL Sialylation of beta1 integrins blocks cell adhesion to galectin-3 and protects cells against galectin-3-induced apoptosis. J. Biol. Chem., 2008;283(32):22177-85. 2008 [PMID: 18676377] (WB,, Human) WB Human

Reviews for Galectin-1 Antibody (AF1152) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using AF1152:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23849393
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23849393

Details

Detection NotesSee PMID 23849393

Comments

CommentsUsed also in cytochemistry.PMID 23849393
 
reviewed by:
Anonymous
WB Human 01/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 24012450
SpeciesHuman

Blocking

Blocking DetailsSee PMID 24012450

Details

Detection NotesSee PMID 24012450

Comments

CommentsPublished in PMID 24012450
 
reviewed by:
Anonymous
WB Human 01/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 19363525
SpeciesHuman

Blocking

Blocking DetailsSee PMID 19363525

Details

Detection NotesSee PMID 19363525

Comments

CommentsSee PMID 19363525

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

 
Anonymous
01/05/2015
Application: WB
Species: Human

 
Anonymous
01/05/2015
Application: WB
Species: Human

 
Anonymous
01/05/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol LGALS1
Entrez
Uniprot