Galectin-8 Antibody [Unconjugated]

Images

 
Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) followed by ...read more
Galectin-8 was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human Galectin-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) at 15 µg/mL overnight at 4 °C. ...read more
Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑8 at approximately 40 kDa (as indicated) using 10 µg/mL of Goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Galectin-8 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Galectin-8
Met1-Trp317
Accession # O00214
Specificity
Detects human Galectin-8 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) rhGalectin-1 and rhGalectin-3 is observed, and less than 1% cross-reactivity with rhGalectin-2, rhGalectin-4 and rhGalectin-7 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LGALS8
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF1305 in the following applications:

Publications
Read Publications using
AF1305 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-8 Antibody [Unconjugated]

  • GAL8
  • Gal-8
  • galectin 8
  • Galectin8
  • Galectin-8
  • galectin-8g
  • lectin, galactoside-binding, soluble, 8
  • LGALS8
  • PCTA1
  • PCTA-1Po66 carbohydrate-binding protein
  • Po66 carbohydrate binding protein
  • Po66-CBP
  • Prostate carcinoma tumor antigen 1

Background

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer. The chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain, and the tandem-repeat Galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1‑4).

Galectin-8, also known as prostate carcinoma tumor antigen 1 (PCTA1) in human, is a tandem repeat-type galectin. Prototype (single CRD) isoforms arising through alternate gene splicing have also been identified (5). Galectin-8 is highly expressed in lung carcinomas, certain forms of prostate carcinomas, as well as other tumor cells. It binds to a subset of cell surface integrins to modulate ECM-integrin interactions. As a soluble ligand, Galectin-8 can inhibit cell adhesion (6). Immobilized Galectin-8, however, has also been shown to promote cell adhesion (7). Human and mouse Galectin-8 share approximately 80% amino acid homology (4).

  1. Rabinovich, A. et al. (2002) TRENDS in Immunol. 23:313.
  2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
  3. Hughes, R.C. (2002) Biochimie 83:667.
  4. R&D Systems’ Cytokine Bulletin, Summer, 2002.
  5. Bidon, N. et al. (2001) Gene 274:253.
  6. Hadari, Y. et al. (1995) J. Biol. Chem. 270:3447.
  7. Levy, Y. et al. (2001) J. Biol. Chem. 276:31285.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Galectin-8 Antibody (AF1305)(30)

We have publications tested in 4 confirmed species: Human, Mouse, Cercopithecus aethiops, Complex Species Category.

We have publications tested in 6 applications: ELISA Development (Detection), Flow Cytometry, ICC, IHC-P, Immunoprecipitation, Western Blot.


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ELISA Development (Detection)
(1)
Flow Cytometry
(2)
ICC
(13)
IHC-P
(2)
Immunoprecipitation
(1)
Western Blot
(6)
All Applications
Filter By Species
Human
(14)
Mouse
(2)
Cercopithecus aethiops
(1)
Complex Species Category
(1)
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Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using AF1305 Applications Species
Bass, AR;Egan, MS;Alexander-Floyd, J;Lopes Fischer, N;Doerner, J;Shin, S; Human GBP1 facilitates the rupture of the Legionella-containing vacuole and inflammasome activation mBio 2023-09-22 [PMID: 37737612] (ICC, Human) ICC Human
Keith B Boyle, Cara J Ellison, Paul R Elliott, Martina Schuschnig, Krista Grimes, Marc S Dionne et al. TECPR1 conjugates LC3 to damaged endomembranes upon detection of sphingomyelin exposure The EMBO Journal 2023-09-04 [PMID: 37409490]
Antonio J. Pagán, Lauren J. Lee, Joy Edwards-Hicks, Cecilia B. Moens, David M. Tobin, Elisabeth M. Busch-Nentwich et al. mTOR-regulated mitochondrial metabolism limits mycobacterium-induced cytotoxicity Cell 2022-09-29 [PMID: 36103894]
Noémie Pied, Coralie F. Daussy, Zoé Denis, Jessica Ragues, Muriel Faure, Richard Iggo et al. TBK1 is part of a galectin 8 dependent membrane damage recognition complex and drives autophagy upon Adenovirus endosomal escape PLOS Pathogens 2022-07-20 [PMID: 35857795]
MM Osman, JK Shanahan, F Chu, KK Takaki, ML Pinckert, AJ Pagán, R Brosch, WH Conrad, L Ramakrishn The C terminus of the mycobacterium ESX-1 secretion system substrate ESAT-6 is required for phagosomal membrane damage and virulence Proceedings of the National Academy of Sciences of the United States of America, 2022-03-10;119(11):e2122161119. 2022-03-10 [PMID: 35271388] (ICC, Human) ICC Human
I Pablos, Y Machado, HCR de Jesus, Y Mohamud, R Kappelhoff, C Lindskog, M Vlok, PA Bell, GS Butler, PM Grin, QT Cao, JP Nguyen, N Solis, S Abbina, W Rut, JC Vederas, L Szekely, A Szakos, M Drag, JN Kizhakkeda, K Mossman, JA Hirota, E Jan, H Luo, A Banerjee, CM Overall Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CLpro substrate degradome Cell Reports, 2021-10-09;37(4):109892. 2021-10-09 [PMID: 34672947] (Western Blot, Cercopithecus aethiops, Human) Western Blot Cercopithecus aethiops, Human
Vinay V Eapen, Sharan Swarup, Melissa J Hoyer, Joao A Paulo, J Wade Harper Quantitative proteomics reveals the selectivity of ubiquitin-binding autophagy receptors in the turnover of damaged lysosomes by lysophagy eLife 2021-09-29 [PMID: 34585663]
EG Otten, E Werner, A Crespillo-, KB Boyle, V Dharamdasa, C Pathe, B Santhanam, F Randow Ubiquitylation of lipopolysaccharide by RNF213 during bacterial infection Nature, 2021-05-19;0(0):. 2021-05-19 [PMID: 34012115] (ICC, Complex Species Category) ICC Complex Species Category
DC Badgujar, A Anil, AE Green, MV Surve, S Madhavan, A Beckett, IA Prior, BK Godsora, SB Patil, PK More, SG Sarkar, A Mitchell, R Banerjee, PS Phale, TJ Mitchell, DR Neill, P Bhaumik, A Banerjee Structural insights into loss of function of a pore forming toxin and its role in pneumococcal adaptation to an intracellular lifestyle PloS Pathogens, 2020-11-20;16(11):e1009016. 2020-11-20 [PMID: 33216805] (ICC, Human) ICC Human
D Fisch, B Clough, MC Domart, V Encheva, H Bando, AP Snijders, LM Collinson, M Yamamoto, AR Shenoy, EM Frickel Human GBP1 Differentially Targets Salmonella and Toxoplasma to License Recognition of Microbial Ligands and Caspase-Mediated Death Cell Rep, 2020-08-11;32(6):108008. 2020-08-11 [PMID: 32783936] (ICC, Western Blot, Human) ICC, Western Blot Human
Show All 30 Publications.

Review for Galectin-8 Antibody (AF1305) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1305:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Human 01/05/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 20876211
SpeciesHuman

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Verified Customer
01/05/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol LGALS8
Uniprot