Galectin-8 Antibody [Unconjugated]

Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Galectin‑8 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Galectin‑8 was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human Galectin‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑8 at approximately 40 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Galectin-8 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Galectin-8
  Met1-Trp317
  Accession # O00214
• Specificity: Detects human Galectin-8 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) rhGalectin-1 and rhGalectin-3 is observed, and less than 1% cross-reactivity with rhGalectin-2, rhGalectin-4 and rhGalectin-7 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LGALS8
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-8 Antibody [Unconjugated]

• GAL8
• Gal-8
• galectin 8
• Galectin8
• Galectin-8
• galectin-8g
• lectin, galactoside-binding, soluble, 8
• LGALS8
• PCTA1
• PCTA-1Po66 carbohydrate-binding protein
• Po66 carbohydrate binding protein
• Po66-CBP
• Prostate carcinoma tumor antigen 1

Background

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer. The chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain, and the tandem-repeat Galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1‑4).

Galectin-8, also known as prostate carcinoma tumor antigen 1 (PCTA1) in human, is a tandem repeat-type galectin. Prototype (single CRD) isoforms arising through alternate gene splicing have also been identified (5). Galectin-8 is highly expressed in lung carcinomas, certain forms of prostate carcinomas, as well as other tumor cells. It binds to a subset of cell surface integrins to modulate ECM-integrin interactions. As a soluble ligand, Galectin-8 can inhibit cell adhesion (6). Immobilized Galectin-8, however, has also been shown to promote cell adhesion (7). Human and mouse Galectin-8 share approximately 80% amino acid homology (4).

1. Rabinovich, A. et al. (2002) TRENDS in Immunol. 23:313.
2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
3. Hughes, R.C. (2002) Biochimie 83:667.
4. R&D Systems’ Cytokine Bulletin, Summer, 2002.
5. Bidon, N. et al. (2001) Gene 274:253.
6. Hadari, Y. et al. (1995) J. Biol. Chem. 270:3447.
7. Levy, Y. et al. (2001) J. Biol. Chem. 276:31285.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Galectin-8 Antibody (AF1305)(17)

Publications using AF1305

• MM Osman, JK Shanahan, F Chu, KK Takaki, ML Pinckert, AJ Pagán, R Brosch, WH Conrad, L Ramakrishn The C terminus of the mycobacterium ESX-1 secretion system substrate ESAT-6 is required for phagosomal membrane damage and virulence Proceedings of the National Academy of Sciences of the United States of America, 2022;119(11):e2122161119. 2022 [PMID: 35271388] (ICC, Human)
• I Pablos, Y Machado, HCR de Jesus, Y Mohamud, R Kappelhoff, C Lindskog, M Vlok, PA Bell, GS Butler, PM Grin, QT Cao, JP Nguyen, N Solis, S Abbina, W Rut, JC Vederas, L Szekely, A Szakos, M Drag, JN Kizhakkeda, K Mossman, JA Hirota, E Jan, H Luo, A Banerjee, CM Overall Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CLpro substrate degradome Cell Reports, 2021;37(4):109892. 2021 [PMID: 34672947] (Western Blot, Cercopithecus aethiops)
• EG Otten, E Werner, A Crespillo-, KB Boyle, V Dharamdasa, C Pathe, B Santhanam, F Randow Ubiquitylation of lipopolysaccharide by RNF213 during bacterial infection Nature, 2021;0(0):. 2021 [PMID: 34012115] (Western Blot, Human)
• DC Badgujar, A Anil, AE Green, MV Surve, S Madhavan, A Beckett, IA Prior, BK Godsora, SB Patil, PK More, SG Sarkar, A Mitchell, R Banerjee, PS Phale, TJ Mitchell, DR Neill, P Bhaumik, A Banerjee Structural insights into loss of function of a pore forming toxin and its role in pneumococcal adaptation to an intracellular lifestyle PloS Pathogens, 2020;16(11):e1009016. 2020 [PMID: 33216805] (ICC, Human)
• D Fisch, B Clough, MC Domart, V Encheva, H Bando, AP Snijders, LM Collinson, M Yamamoto, AR Shenoy, EM Frickel Human GBP1 Differentially Targets Salmonella and Toxoplasma to License Recognition of Microbial Ligands and Caspase-Mediated Death Cell Rep, 2020;32(6):108008. 2020 [PMID: 32783936] (ICC, Human)
• D Fisch, B Clough, MC Domart, V Encheva, H Bando, AP Snijders, LM Collinson, M Yamamoto, AR Shenoy, EM Frickel Human GBP1 Differentially Targets Salmonella and Toxoplasma to License Recognition of Microbial Ligands and Caspase-Mediated Death Cell Rep, 2020;32(6):108008. 2020 [PMID: 32783936] (Western Blot, Human)
• MM Osman, AJ Pagán, JK Shanahan, L Ramakrishn Mycobacterium marinum phthiocerol dimycocerosates enhance macrophage phagosomal permeabilization and membrane damage PLoS ONE, 2020;15(7):e0233252. 2020 [PMID: 32701962] (ICC, Human)
• Ž Boji?-Trbo, M Jovanovi?, A Viloti?, N Kolundži?, I Stefanoska, F Zetterberg, UJ Nilsson, H Leffler, L Vi?ovac Human trophoblast requires galectin-3 for cell migration and invasion Sci Rep, 2019;9(1):2136. 2019 [PMID: 30765738] (ELISA Development (Detection), Human)
• TR Lerner, CJ Queval, A Fearns, U Repnik, G Griffiths, MG Gutierrez Phthiocerol dimycocerosates promote access to the cytosol and intracellular burden of Mycobacterium tuberculosis in lymphatic endothelial cells BMC Biol., 2018;16(1):1. 2018 [PMID: 29325545] (ICC, Human)
• Falcon B, Noad J, McMahon H, Randow F, Goedert M Galectin-8-mediated selective autophagy protects against seeded tau aggregation. J Biol Chem, 0;293(7):2438-2451. 0 [PMID: 29282296] (ICC, Human)
• L Schnettger, A Rodgers, U Repnik, RP Lai, G Pei, M Verdoes, RJ Wilkinson, DB Young, MG Gutierrez A Rab20-Dependent Membrane Trafficking Pathway Controls M.�tuberculosis Replication by Regulating Phagosome Spaciousness and Integrity Cell Host Microbe, 2017;21(5):619-628.e5. 2017 [PMID: 28494243] (ICC, Mouse)
• K63-Linked Ubiquitination Targets Toxoplasma gondii for Endo-lysosomal Destruction in IFN?-Stimulated Human Cells PLoS Pathog, 2016;12(11):e1006027. 2016 [PMID: 27875583] (ICC, Human)
• Meunier E, Dick M, Dreier R, Schurmann N, Kenzelmann Broz D, Warming S, Roose-Girma M, Bumann D, Kayagaki N, Takeda K, Yamamoto M, Broz P Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases. Nature, 2014;509(7500):366-70. 2014 [PMID: 24739961] (ICC, Mouse)
• Diskin S, Chen WS, Cao Z, Gyawali S, Gong H, Soza A, Gonzalez A, Panjwani N Galectin-8 Promotes Cytoskeletal Rearrangement in Trabecular Meshwork Cells through Activation of Rho Signaling. PLoS ONE, 2012;7(9):e44400. 2012 [PMID: 22973445] (Western Blot, Human)
• Delgado VM, Nugnes LG, Colombo LL, Troncoso MF, Fernandez MM, Malchiodi EL, Frahm I, Croci DO, Compagno D, Rabinovich GA, Wolfenstein-Todel C, Elola MT Modulation of endothelial cell migration and angiogenesis: a novel function for the "tandem-repeat" lectin galectin-8. FASEB J., 2011;25(1):242-54. 2011 [PMID: 20876211] (Western Blot, Human)
• Cueni LN, Detmar M Galectin-8 interacts with podoplanin and modulates lymphatic endothelial cell functions. Exp. Cell Res., 2009;315(10):1715-23. 2009 [PMID: 19268462] (Immunoprecipitation, Human)
• Thijssen VL, Hulsmans S, Griffioen AW The galectin profile of the endothelium: altered expression and localization in activated and tumor endothelial cells. Am. J. Pathol., 2008;172(2):545-53. 2008 [PMID: 18202194] (Western Blot, Human)

Review for Galectin-8 Antibody (AF1305) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous - WB Human 01/05/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 20876211
• Species: Human

Bioinformatics

• Gene Symbol: LGALS8
• Uniprot:
  • O00214()