Galectin-8 Antibody [Unconjugated]


Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) followed by more
Galectin‑8 was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human Galectin‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) at 15 µg/mL overnight at 4 more
Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑8 at approximately 40 kDa (as indicated) using 10 µg/mL of Goat more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC

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Galectin-8 Antibody [Unconjugated] Summary

E. coli-derived recombinant human Galectin-8
Accession # O00214
Detects human Galectin-8 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) rhGalectin-1 and rhGalectin-3 is observed, and less than 1% cross-reactivity with rhGalectin-2, rhGalectin-4 and rhGalectin-7 is observed.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
AF1305 in the following applications:

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AF1305 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-8 Antibody [Unconjugated]

  • GAL8
  • Gal-8
  • galectin 8
  • Galectin8
  • Galectin-8
  • galectin-8g
  • lectin, galactoside-binding, soluble, 8
  • LGALS8
  • PCTA1
  • PCTA-1Po66 carbohydrate-binding protein
  • Po66 carbohydrate binding protein
  • Po66-CBP
  • Prostate carcinoma tumor antigen 1


The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer. The chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain, and the tandem-repeat Galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1‑4).

Galectin-8, also known as prostate carcinoma tumor antigen 1 (PCTA1) in human, is a tandem repeat-type galectin. Prototype (single CRD) isoforms arising through alternate gene splicing have also been identified (5). Galectin-8 is highly expressed in lung carcinomas, certain forms of prostate carcinomas, as well as other tumor cells. It binds to a subset of cell surface integrins to modulate ECM-integrin interactions. As a soluble ligand, Galectin-8 can inhibit cell adhesion (6). Immobilized Galectin-8, however, has also been shown to promote cell adhesion (7). Human and mouse Galectin-8 share approximately 80% amino acid homology (4).

  1. Rabinovich, A. et al. (2002) TRENDS in Immunol. 23:313.
  2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
  3. Hughes, R.C. (2002) Biochimie 83:667.
  4. R&D Systems’ Cytokine Bulletin, Summer, 2002.
  5. Bidon, N. et al. (2001) Gene 274:253.
  6. Hadari, Y. et al. (1995) J. Biol. Chem. 270:3447.
  7. Levy, Y. et al. (2001) J. Biol. Chem. 276:31285.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Galectin-8 Antibody (AF1305)(17)

We have publications tested in 3 confirmed species: Human, Mouse, Cercopithecus aethiops.

We have publications tested in 4 applications: ELISA Development (Detection), ICC, Immunoprecipitation, Western Blot.

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ELISA Development (Detection)
Western Blot
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Cercopithecus aethiops
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Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using AF1305 Applications Species
MM Osman, JK Shanahan, F Chu, KK Takaki, ML Pinckert, AJ Pagán, R Brosch, WH Conrad, L Ramakrishn The C terminus of the mycobacterium ESX-1 secretion system substrate ESAT-6 is required for phagosomal membrane damage and virulence Proceedings of the National Academy of Sciences of the United States of America, 2022;119(11):e2122161119. 2022 [PMID: 35271388] (ICC, Human) ICC Human
I Pablos, Y Machado, HCR de Jesus, Y Mohamud, R Kappelhoff, C Lindskog, M Vlok, PA Bell, GS Butler, PM Grin, QT Cao, JP Nguyen, N Solis, S Abbina, W Rut, JC Vederas, L Szekely, A Szakos, M Drag, JN Kizhakkeda, K Mossman, JA Hirota, E Jan, H Luo, A Banerjee, CM Overall Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CLpro substrate degradome Cell Reports, 2021;37(4):109892. 2021 [PMID: 34672947] (Western Blot, Cercopithecus aethiops) Western Blot Cercopithecus aethiops
EG Otten, E Werner, A Crespillo-, KB Boyle, V Dharamdasa, C Pathe, B Santhanam, F Randow Ubiquitylation of lipopolysaccharide by RNF213 during bacterial infection Nature, 2021;0(0):. 2021 [PMID: 34012115] (Western Blot, Human) Western Blot Human
DC Badgujar, A Anil, AE Green, MV Surve, S Madhavan, A Beckett, IA Prior, BK Godsora, SB Patil, PK More, SG Sarkar, A Mitchell, R Banerjee, PS Phale, TJ Mitchell, DR Neill, P Bhaumik, A Banerjee Structural insights into loss of function of a pore forming toxin and its role in pneumococcal adaptation to an intracellular lifestyle PloS Pathogens, 2020;16(11):e1009016. 2020 [PMID: 33216805] (ICC, Human) ICC Human
D Fisch, B Clough, MC Domart, V Encheva, H Bando, AP Snijders, LM Collinson, M Yamamoto, AR Shenoy, EM Frickel Human GBP1 Differentially Targets Salmonella and Toxoplasma to License Recognition of Microbial Ligands and Caspase-Mediated Death Cell Rep, 2020;32(6):108008. 2020 [PMID: 32783936] (ICC, Human) ICC Human
D Fisch, B Clough, MC Domart, V Encheva, H Bando, AP Snijders, LM Collinson, M Yamamoto, AR Shenoy, EM Frickel Human GBP1 Differentially Targets Salmonella and Toxoplasma to License Recognition of Microbial Ligands and Caspase-Mediated Death Cell Rep, 2020;32(6):108008. 2020 [PMID: 32783936] (Western Blot, Human) Western Blot Human
MM Osman, AJ Pagán, JK Shanahan, L Ramakrishn Mycobacterium marinum phthiocerol dimycocerosates enhance macrophage phagosomal permeabilization and membrane damage PLoS ONE, 2020;15(7):e0233252. 2020 [PMID: 32701962] (ICC, Human) ICC Human
Ž Boji?-Trbo, M Jovanovi?, A Viloti?, N Kolundži?, I Stefanoska, F Zetterberg, UJ Nilsson, H Leffler, L Vi?ovac Human trophoblast requires galectin-3 for cell migration and invasion Sci Rep, 2019;9(1):2136. 2019 [PMID: 30765738] (ELISA Development (Detection), Human) ELISA Development (Detection) Human
TR Lerner, CJ Queval, A Fearns, U Repnik, G Griffiths, MG Gutierrez Phthiocerol dimycocerosates promote access to the cytosol and intracellular burden of Mycobacterium tuberculosis in lymphatic endothelial cells BMC Biol., 2018;16(1):1. 2018 [PMID: 29325545] (ICC, Human) ICC Human
Falcon B, Noad J, McMahon H, Randow F, Goedert M Galectin-8-mediated selective autophagy protects against seeded tau aggregation. J Biol Chem, 0;293(7):2438-2451. 0 [PMID: 29282296] (ICC, Human) ICC Human
Show All 17 Publications.

Review for Galectin-8 Antibody (AF1305) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1305:
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Images Ratings Applications Species Date Details
reviewed by:
Anonymous -
WB Human 01/05/2015


ApplicationWestern Blot
Sample TestedSee PMID 20876211

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Recent Reviews


Anonymous -
Application: WB
Species: Human


Gene Symbol LGALS8