Galectin-8 Antibody [Unconjugated]

Images

 
Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) followed by ...read more
Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑8 at approximately 40 kDa (as indicated) using 10 µg/mL of Goat ...read more
Galectin‑8 was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human Galectin‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) at 15 µg/mL overnight at 4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price

Galectin-8 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Galectin-8
Met1-Trp317
Accession # O00214
Specificity
Detects human Galectin-8 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) rhGalectin-1 and rhGalectin-3 is observed, and less than 1% cross-reactivity with rhGalectin-2, rhGalectin-4 and rhGalectin-7 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LGALS8
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF1305 in the following applications:

Publications
Read Publications using
AF1305 in the following applications:

  • ICC
    2 publications
  • IP
    1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-8 Antibody [Unconjugated]

  • GAL8
  • Gal-8
  • galectin 8
  • Galectin8
  • Galectin-8
  • galectin-8g
  • lectin, galactoside-binding, soluble, 8
  • LGALS8
  • PCTA1
  • PCTA-1Po66 carbohydrate-binding protein
  • Po66 carbohydrate binding protein
  • Po66-CBP
  • Prostate carcinoma tumor antigen 1

Background

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer. The chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain, and the tandem-repeat Galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1‑4).

Galectin-8, also known as prostate carcinoma tumor antigen 1 (PCTA1) in human, is a tandem repeat-type galectin. Prototype (single CRD) isoforms arising through alternate gene splicing have also been identified (5). Galectin-8 is highly expressed in lung carcinomas, certain forms of prostate carcinomas, as well as other tumor cells. It binds to a subset of cell surface integrins to modulate ECM-integrin interactions. As a soluble ligand, Galectin-8 can inhibit cell adhesion (6). Immobilized Galectin-8, however, has also been shown to promote cell adhesion (7). Human and mouse Galectin-8 share approximately 80% amino acid homology (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Galectin-8 Antibody (AF1305)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC, IP, WB.


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ICC
(2)
IP
(1)
WB
(3)
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Human
(5)
Mouse
(1)
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Showing Publications 1 - 6 of 6.
Publications using AF1305 Applications Species
K63-Linked Ubiquitination Targets Toxoplasma gondii for Endo-lysosomal Destruction in IFN?-Stimulated Human Cells PLoS Pathog, 2016;12(11):e1006027. 2016 [PMID: 27875583] (ICC,, Human) ICC Human
Meunier E, Dick M, Dreier R, Schurmann N, Kenzelmann Broz D, Warming S, Roose-Girma M, Bumann D, Kayagaki N, Takeda K, Yamamoto M, Broz P Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases. Nature, 2014;509(7500):366-70. 2014 [PMID: 24739961] (ICC,, Mouse) ICC Mouse
Diskin S, Chen WS, Cao Z, Gyawali S, Gong H, Soza A, Gonzalez A, Panjwani N Galectin-8 Promotes Cytoskeletal Rearrangement in Trabecular Meshwork Cells through Activation of Rho Signaling. PLoS ONE, 2012;7(9):e44400. 2012 [PMID: 22973445] (WB,, Human) WB Human
Delgado VM, Nugnes LG, Colombo LL, Troncoso MF, Fernandez MM, Malchiodi EL, Frahm I, Croci DO, Compagno D, Rabinovich GA, Wolfenstein-Todel C, Elola MT Modulation of endothelial cell migration and angiogenesis: a novel function for the "tandem-repeat" lectin galectin-8. FASEB J., 2011;25(1):242-54. 2011 [PMID: 20876211] (WB,, Human) WB Human
Cueni LN, Detmar M Galectin-8 interacts with podoplanin and modulates lymphatic endothelial cell functions. Exp. Cell Res., 2009;315(10):1715-23. 2009 [PMID: 19268462] (IP,, Human) IP Human
Thijssen VL, Hulsmans S, Griffioen AW The galectin profile of the endothelium: altered expression and localization in activated and tumor endothelial cells. Am. J. Pathol., 2008;172(2):545-53. 2008 [PMID: 18202194] (WB,, Human) WB Human

Review for Galectin-8 Antibody (AF1305) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1305:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/05/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 20876211
SpeciesHuman

Blocking

Blocking DetailsSee PMID 20876211

Details

Detection NotesSee PMID 20876211

Comments

CommentsVery SpecificSee PMID 20876211

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Recent Reviews

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Anonymous
01/05/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol LGALS8
Entrez
Uniprot