TIM-1/KIM-1/HAVCR Antibody [Unconjugated]

Images

 
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse TIM‑1/KIM‑1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

TIM-1/KIM-1/HAVCR Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TIM‑1/KIM-1/HAVCR
Tyr22-Thr212
Accession # NP_001160104
Specificity
Detects mouse TIM‑1/KIM-1/HAVCR in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human TIM-1, recombinant mouse (rm) TIM-2, rmTIM-3, rmTIM-4, rmTIM-6, and rmTIM-7 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HAVCR1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
Publications
Read Publications using
AF1817 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-1/KIM-1/HAVCR Antibody [Unconjugated]

  • CD365
  • HAVCR1
  • HAVCR-1
  • HAVCRT cell immunoglobin domain and mucin domain protein 1
  • hepatitis A virus cellular receptor 1
  • Kidney injury molecule 1
  • KIM1
  • KIM-1
  • T-cell immunoglobulin and mucin domain-containing protein 1
  • TIM1
  • TIM-1
  • TIM-1TIM
  • TIM1TIMD-1
  • TIMD1T-cell membrane protein 1

Background

TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVCR) is a 70-80 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-4). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM genes (# 1-8) vs. only three genes in human (# 1, 3, and 4) (1, 2). Mouse TIM-1 and -2 are counterparts of human TIM-1 while mouse TIM-5 through 8 have no human counterparts (2). Mouse TIM-1 is synthesized as a 305 amino acid (aa) precursor that contains a 21 aa signal sequence, a 216 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (5, 6). The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple T-S-P motifs. The mucin region undergoes extensive O-linked glycosylation. The mouse TIM-1 gene is highly polymorphic and, based on rat, may undergo alternate splicing (4, 6). For instance, HBA mice show a 15 aa deletion in the mucin region that occurs in BALB/c mice (6). This difference is associated with a decreased susceptibility to asthma. Other polymorphisms are also documented (6). In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing a 75-85 kDa soluble molecule (5). The same thing might be expected in mouse. The ECD of mouse TIM-1 is 50%, 39%, and 80% aa identical to human, canine and rat TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (8, 9). However, others are believed to exist, and based on the ligand for TIM-3, one possibility might be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10). In particular, it induces IL-4 production, and requires the cytoplasmic tyrosine phosphorylation motif (5).

  1. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471. 
  2. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454. 
  3. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.  
  4. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.  
  5. de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113. 
  6. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
  7. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
  8. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
  9. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  10. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TIM-1/KIM-1/HAVCR Antibody (AF1817)(14)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Flow, IHC, IHC Frozen, IHC Paraffin-embedded, IHC-P, WB.


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Flow
(1)
IHC
(2)
IHC Frozen
(1)
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(1)
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(1)
WB
(8)
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(1)
Mouse
(13)
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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF1817 Applications Species
E Lazzeri, ML Angelotti, A Peired, C Conte, JA Marschner, L Maggi, B Mazzinghi, D Lombardi, ME Melica, S Nardi, E Ronconi, A Sisti, G Antonelli, F Becherucci, L De Chiara, RR Guevara, A Burger, B Schaefer, F Annunziato, HJ Anders, L Lasagni, P Romagnani Endocycle-related tubular cell hypertrophy and progenitor proliferation recover renal function after acute kidney injury Nat Commun, 2018;9(1):1344. 2018 [PMID: 29632300] (IHC,, Mouse) IHC Mouse
O Maydan, PG McDade, Y Liu, XR Wu, DG Matsell, AA Eddy Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy Physiol Rep, 2018;6(6):e13654. 2018 [PMID: 29595914] (WB,, Mouse) WB Mouse
Y Kim, SJ Park, SR Manson, CA Molina, K Kidd, H Thiessen-P, RJ Perry, H Liapis, S Kmoch, CR Parikh, AJ Bleyer, YM Chen Elevated urinary CRELD2 is associated with endoplasmic reticulum stress-mediated kidney disease JCI Insight, 2017;2(23):. 2017 [PMID: 29212948] (WB,, Mouse) WB Mouse
C Tang, H Han, M Yan, S Zhu, J Liu, Z Liu, L He, J Tan, Y Liu, H Liu, L Sun, S Duan, Y Peng, F Liu, XM Yin, Z Zhang, Z Dong PINK1-PRKN/PARK2 pathway of mitophagy is activated to protect against Renal ischemia-reperfusion injury Autophagy, 2017;0(0):1-45. 2017 [PMID: 29172924] (WB,, Mouse) WB Mouse
J Liu, S Kumar, E Dolzhenko, GF Alvarado, J Guo, C Lu, Y Chen, M Li, MC Dessing, RK Parvez, PE Cippà, AM Krautzberg, G Saribekyan, AD Smith, AP McMahon Molecular characterization of the transition from acute to chronic kidney injury following ischemia/reperfusion JCI Insight, 2017;2(18):. 2017 [PMID: 28931758] (IHC-P,, Mouse) IHC-P Mouse
L Li, R Zviti, C Ha, ZV Wang, JA Hill, F Lin Forkhead Box O3 (FoxO3) Regulates Kidney Tubular Autophagy Following Urinary Tract Obstruction J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28705935] (IHC,, Mouse) IHC Mouse
JB Collier, RM Whitaker, ST Eblen, RG Schnellman Rapid Renal Regulation of Peroxisome Proliferator-activated Receptor ? Coactivator-1? by Extracellular Signal-Regulated Kinase 1/2 in Physiological and Pathological Conditions J. Biol. Chem., 2016;291(52):26850-26859. 2016 [PMID: 27875304] (WB,, Mouse) WB Mouse
Rescue therapy with Tanshinone IIA hinders transition of acute kidney injury to chronic kidney disease via targeting GSK3? Sci Rep, 2016;6(0):36698. 2016 [PMID: 27857162] (IHC Frozen,, Mouse) IHC Frozen Mouse
Wnt4 is a novel biomarker for the early detection of kidney tubular injury after ischemia/reperfusion injury Sci Rep, 2016;6(0):32610. 2016 [PMID: 27600466] (WB,, Mouse) WB Mouse
Ishida R, Kami D, Kusaba T, Kirita Y, Kishida T, Mazda O, Adachi T, Gojo S Kidney-specific Sonoporation-mediated Gene Transfer. Mol Ther, 2016;24(1):125-34. 2016 [PMID: 26419704] (WB,, Mouse) WB Mouse
Show All 14 Publications.

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Bioinformatics

Gene Symbol HAVCR1
Entrez
Uniprot