TIM-1/KIM-1/HAVCR Antibody [Unconjugated]

Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse TIM‑1/KIM‑1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1817) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TIM‑1/KIM‑1/HAVCR at approximately70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TIM-1/KIM-1/HAVCR Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TIM‑1/KIM-1/HAVCR
  Tyr22-Thr212
  Accession # NP_001160104
• Specificity: Detects mouse TIM‑1/KIM-1/HAVCR in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human TIM-1, recombinant mouse (rm) TIM-2, rmTIM-3, rmTIM-4, rmTIM-6, and rmTIM-7 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HAVCR1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-1/KIM-1/HAVCR Antibody [Unconjugated]

• CD365
• HAVCR1
• HAVCR-1
• HAVCRT cell immunoglobin domain and mucin domain protein 1
• hepatitis A virus cellular receptor 1
• Kidney injury molecule 1
• KIM1
• KIM-1
• T-cell immunoglobulin and mucin domain-containing protein 1
• TIM1
• TIM-1
• TIM-1TIM
• TIM1TIMD-1
• TIMD1T-cell membrane protein 1

Background

TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVCR) is a 70-80 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-4). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM genes (# 1-8) vs. only three genes in human (# 1, 3, and 4) (1, 2). Mouse TIM-1 and -2 are counterparts of human TIM-1 while mouse TIM-5 through 8 have no human counterparts (2). Mouse TIM-1 is synthesized as a 305 amino acid (aa) precursor that contains a 21 aa signal sequence, a 216 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (5, 6). The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple T-S-P motifs. The mucin region undergoes extensive O-linked glycosylation. The mouse TIM-1 gene is highly polymorphic and, based on rat, may undergo alternate splicing (4, 6). For instance, HBA mice show a 15 aa deletion in the mucin region that occurs in BALB/c mice (6). This difference is associated with a decreased susceptibility to asthma. Other polymorphisms are also documented (6). In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing a 75-85 kDa soluble molecule (5). The same thing might be expected in mouse. The ECD of mouse TIM-1 is 50%, 39%, and 80% aa identical to human, canine and rat TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (8, 9). However, others are believed to exist, and based on the ligand for TIM-3, one possibility might be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10). In particular, it induces IL-4 production, and requires the cytoplasmic tyrosine phosphorylation motif (5).

1. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471. 
2. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454. 
3. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.  
4. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.  
5. de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113. 
6. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
7. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
8. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
9. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
10. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIM-1/KIM-1/HAVCR Antibody (AF1817)(39)

Publications using AF1817

• K Hoeft, GJL Schaefer, H Kim, D Schumacher, T Bleckwehl, Q Long, BM Klinkhamme, F Peisker, L Koch, J Nagai, M Halder, S Ziegler, E Liehn, C Kuppe, J Kranz, S Menzel, I Costa, A Wahida, P Boor, RK Schneider, S Hayat, R Kramann Platelet-instructed SPP1+ macrophages drive myofibroblast activation in fibrosis in a CXCL4-dependent manner Cell Reports, 2023-02-17;42(2):112131. 2023-02-17 [PMID: 36807143] (IHC, Mouse)
• Z Zhao, G Li, Y Wang, Y Li, H Xu, W Liu, W Hao, Y Yao, R Zeng Cytoplasmic HMGB1 induces renal tubular ferroptosis after ischemia/reperfusion International immunopharmacology, 2023-02-01;116(0):109757. 2023-02-01 [PMID: 36731154] (IHC, Transgenic Mouse)
• X Cao, J Wang, T Zhang, Z Liu, L Liu, Y Chen, Z Li, Y Zhao, Q Yu, T Liu, J Nie, Y Niu, Y Chen, L Yang, L Zhang Chromatin accessibility dynamics dictate renal tubular epithelial cell response to injury Nature Communications, 2022-11-28;13(1):7322. 2022-11-28 [PMID: 36443310] (IHC, Mouse)
• S Ide, K Ide, K Abe, Y Kobayashi, H Kitai, J McKey, SA Strausser, LL O'Brien, A Tata, PR Tata, T Souma Sex differences in resilience to ferroptosis underlie sexual dimorphism in kidney injury and repair Cell Reports, 2022-11-08;41(6):111610. 2022-11-08 [PMID: 36351395] (IHC, Transgenic Mouse)
• LS Dalbøge, M Christense, MR Madsen, T Secher, N Endlich, V Drenic', A Manresa-Ar, HH Hansen, I Rune, LN Fink, MV Østergaard Nephroprotective Effects of Semaglutide as Mono- and Combination Treatment with Lisinopril in a Mouse Model of Hypertension-Accelerated Diabetic Kidney Disease Biomedicines, 2022-07-11;10(7):. 2022-07-11 [PMID: 35884965] (IHC, Mouse)
• MJ Livingston, S Shu, Y Fan, Z Li, Q Jiao, XM Yin, MA Venkatacha, Z Dong Tubular cells produce FGF2 via autophagy after acute kidney injury leading to fibroblast activation and renal fibrosis Autophagy, 2022-05-18;0(0):. 2022-05-18 [PMID: 35491858] (IHC, Mouse)
• T Eckes, S Patyna, A Koch, A Oftring, S Gauer, N Obermüller, S Schwalm, L Schaefer, J Chun, HJ Gröne, J Pfeilschif Sphingosine 1-Phosphate Receptor 5 (S1P5) Knockout Ameliorates Adenine-Induced Nephropathy International Journal of Molecular Sciences, 2022-04-02;23(7):. 2022-04-02 [PMID: 35409312] (Western Blot, Mouse)
• TH Shen, J Stauber, K Xu, A Jacunski, N Paragas, M Callahan, R Banlengchi, AD Levitman, B Desanti de, A Beenken, MS Grau, E Mathieu, Q Zhang, Y Li, T Gopal, N Askanase, S Arumugam, S Mohan, PI Good, JS Stevens, F Lin, SK Sia, CS Lin, V D'Agati, K Kiryluk, NP Tatonetti, J Barasch Snapshots of nascent RNA reveal cell- and stimulus- specific responses to acute kidney injury JCI Insight, 2022-03-22;0(0):. 2022-03-22 [PMID: 35230973] (IHC, Mouse)
• EA Telford, AJ Sanders, S Owen, F Ruge, GM Harrison, WG Jiang, TA Martin Hepatitis A Virus Cellular Receptor 1 (HAVcr-1) Initiates Prostate Cancer Progression in Human Cells via Hepatocyte Growth Factor (HGF)-Induced Changes in Junctional Integrity Biomolecules, 2022-02-21;12(2):. 2022-02-21 [PMID: 35204839] (Western Blot, Human)
• S Wang, K Jing, H Wu, X Li, C Yang, T Li, H Tang, T Zou, Y She, HF Liu Activation of Transcription Factor EB Alleviates Tubular Epithelial Cell Injury via Restoring Lysosomal Homeostasis in Diabetic Nephropathy Oxidative Medicine and Cellular Longevity, 2022-01-12;2022(0):2812493. 2022-01-12 [PMID: 35082964] (IHC, Mouse)
• N An, C Yang, HL Wu, Y Guo, XJ Huang, TS Huang, ZH Wu, J Xue, RH Chen, ZH Li, QJ Pan, HF Liu Hydroxychloroquine administration exacerbates acute kidney injury complicated by lupus nephritis Arthritis Research & Therapy, 2022-01-03;24(1):6. 2022-01-03 [PMID: 34980245] (IHC, Mouse)
• AR Schiazza, EG Considine, M Betcher, BD Shepard Loss of renal olfactory receptor 1393 leads to improved glucose homeostasis in a type 1 diabetic mouse model Physiological Reports, 2021-12-01;9(23):e15007. 2021-12-01 [PMID: 34877823] (Western Blot, Mouse)
• N Ma, Z Wei, J Hu, W Gu, X Ci Farrerol Ameliorated Cisplatin-Induced Chronic Kidney Disease Through Mitophagy Induction via Nrf2/PINK1 Pathway Frontiers in Pharmacology, 2021-11-11;12(0):768700. 2021-11-11 [PMID: 34858188] (Western Blot, Mouse, Transgenic Mouse)
• L Mulderrig, JI Garaycoech, ZK Tuong, CL Millington, FA Dingler, JR Ferdinand, L Gaul, JA Tadross, MJ Arends, S O'Rahilly, GP Crossan, MR Clatworthy, KJ Patel Aldehyde-driven transcriptional stress triggers an anorexic DNA damage response Nature, 2021-11-24;600(7887):158-163. 2021-11-24 [PMID: 34819667] (IHC, Mouse)
• YK You, WF Wu, XR Huang, HD Li, YP Ren, JC Zeng, H Chen, HY Lan Deletion of Smad3 protects against C-reactive protein-induced renal fibrosis and inflammation in obstructive nephropathy International journal of biological sciences, 2021-09-21;17(14):3911-3922. 2021-09-21 [PMID: 34671208] (Western Blot, Mouse)
• JC Lee, DM Yotis, JY Lee, MA Sarabusky, B Shrum, A Champagne, OZ Ismail, E Tutunea-Fa, HS Leong, L Gunaratnam Kidney injury molecule-1 inhibits metastasis of renal cell carcinoma Scientific Reports, 2021-06-04;11(1):11840. 2021-06-04 [PMID: 34088927] (Western Blot, Human)
• M Arita, S Watanabe, N Aoki, S Kuwahara, R Suzuki, S Goto, Y Abe, M Takahashi, M Sato, S Hokari, A Ohtsubo, S Shoji, K Nozaki, K Ichikawa, R Kondo, M Hayashi, Y Ohshima, H Kabasawa, M Hosojima, T Koya, A Saito, T Kikuchi Combination therapy of cisplatin with cilastatin enables an increased dose of cisplatin, enhancing its antitumor effect by suppression of nephrotoxicity Scientific Reports, 2021-01-12;11(1):750. 2021-01-12 [PMID: 33437029] (IHC, Mouse)
• Z Li, M Murakoshi, S Ichikawa, T Koshida, E Adachi, C Suzuki, S Ueda, T Gohda, Y Suzuki The sodium-glucose cotransporter 2 inhibitor tofogliflozin prevents diabetic kidney disease progression in type 2 diabetic mice FEBS Open Bio, 2020-11-10;0(0):. 2020-11-10 [PMID: 33098615] (IHC, Mouse)
• R Uni, T Inoue, Y Nakamura, D Fukaya, S Hasegawa, CH Wu, R Fujii, B Suratticha, W Peerapanya, A Ozeki, N Akimitsu, Y Wada, M Nangaku, R Inagi Vagus nerve stimulation even after injury ameliorates cisplatin-induced nephropathy via reducing macrophage infiltration Sci Rep, 2020-06-11;10(1):9472. 2020-06-11 [PMID: 32528023] (IHC, Mouse)
• M Zaidan, M Burtin, JD Zhang, T Blanc, P Barre, S Garbay, C Nguyen, F Vasseur, L Yammine, S Germano, L Badi, MC Gubler, M Gallazzini, G Friedlande, M Pontoglio, F Terzi Signaling pathways predisposing to chronic kidney disease progression JCI Insight, 2020-05-07;5(9):. 2020-05-07 [PMID: 32376805] (IHC, Mouse)
• MØ Nørgård, M Christense, H A M Mutsae, R Nørregaard Phenformin Attenuates Renal Injury in Unilateral Ureteral Obstructed Mice without Affecting Immune Cell Infiltration Pharmaceutics, 2020-03-26;12(4):. 2020-03-26 [PMID: 32224876] (IHC, Mouse)
• M Chang-Pane, FF Kadyrov, M Lalli, H Wu, S Ikeda, E Kefaloyian, MM Abdelmagee, A Herrlich, A Kobayashi, BD Humphreys FOXM1 drives proximal tubule proliferation during repair from acute ischemic kidney injury J. Clin. Invest., 2019-12-02;0(0):. 2019-12-02 [PMID: 31710314] (IHC, Western Blot, Mouse)
• WZ Ying, X Li, S Rangarajan, W Feng, LM Curtis, PW Sanders Immunoglobulin light chains generate proinflammatory and profibrotic kidney injury J. Clin. Invest., 2019-06-17;129(7):2792-2806. 2019-06-17 [PMID: 31205024] (IHC, Mouse)
• C Suzuki, I Tanida, M Ohmuraya, JA Oliva Trej, S Kakuta, T Sunabori, Y Uchiyama Lack of Cathepsin D in the Renal Proximal Tubular Cells Resulted in Increased Sensitivity against Renal Ischemia/Reperfusion Injury Int J Mol Sci, 2019-04-05;20(7):. 2019-04-05 [PMID: 30959855] (ICC, Mouse)
• E Lazzeri, ML Angelotti, A Peired, C Conte, JA Marschner, L Maggi, B Mazzinghi, D Lombardi, ME Melica, S Nardi, E Ronconi, A Sisti, G Antonelli, F Becherucci, L De Chiara, RR Guevara, A Burger, B Schaefer, F Annunziato, HJ Anders, L Lasagni, P Romagnani Endocycle-related tubular cell hypertrophy and progenitor proliferation recover renal function after acute kidney injury Nat Commun, 2018-04-09;9(1):1344. 2018-04-09 [PMID: 29632300] (IHC, Mouse)
• O Maydan, PG McDade, Y Liu, XR Wu, DG Matsell, AA Eddy Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy Physiol Rep, 2018-03-01;6(6):e13654. 2018-03-01 [PMID: 29595914] (IHC, Western Blot, Mouse)
• M Zhao, Y Zhou, S Liu, L Li, Y Chen, J Cheng, Y Lu, J Liu Control release of mitochondria-targeted antioxidant by injectable self-assembling peptide hydrogel ameliorated persistent mitochondrial dysfunction and inflammation after acute kidney injury Drug Deliv, 2018-11-01;25(1):546-554. 2018-11-01 [PMID: 29451033] (IHC-Fr, Mouse)
• Y Kim, SJ Park, SR Manson, CA Molina, K Kidd, H Thiessen-P, RJ Perry, H Liapis, S Kmoch, CR Parikh, AJ Bleyer, YM Chen Elevated urinary CRELD2 is associated with endoplasmic reticulum stress-mediated kidney disease JCI Insight, 2017-12-07;2(23):. 2017-12-07 [PMID: 29212948] (Western Blot, Mouse)
• C Tang, H Han, M Yan, S Zhu, J Liu, Z Liu, L He, J Tan, Y Liu, H Liu, L Sun, S Duan, Y Peng, F Liu, XM Yin, Z Zhang, Z Dong PINK1-PRKN/PARK2 pathway of mitophagy is activated to protect against Renal ischemia-reperfusion injury Autophagy, 2018-02-17;0(0):1-45. 2018-02-17 [PMID: 29172924] (Western Blot, Mouse)
• J Liu, S Kumar, E Dolzhenko, GF Alvarado, J Guo, C Lu, Y Chen, M Li, MC Dessing, RK Parvez, PE Cippà, AM Krautzberg, G Saribekyan, AD Smith, AP McMahon Molecular characterization of the transition from acute to chronic kidney injury following ischemia/reperfusion JCI Insight, 2017-09-21;2(18):. 2017-09-21 [PMID: 28931758] (IHC-P, Mouse)
• L Li, R Zviti, C Ha, ZV Wang, JA Hill, F Lin Forkhead Box O3 (FoxO3) Regulates Kidney Tubular Autophagy Following Urinary Tract Obstruction J. Biol. Chem., 2017-07-13;0(0):. 2017-07-13 [PMID: 28705935] (IHC, Mouse)
• JB Collier, RM Whitaker, ST Eblen, RG Schnellman Rapid Renal Regulation of Peroxisome Proliferator-activated Receptor ? Coactivator-1? by Extracellular Signal-Regulated Kinase 1/2 in Physiological and Pathological Conditions J. Biol. Chem., 2016-11-14;291(52):26850-26859. 2016-11-14 [PMID: 27875304] (Western Blot, Mouse)
• Rescue therapy with Tanshinone IIA hinders transition of acute kidney injury to chronic kidney disease via targeting GSK3? Sci Rep, 2016-11-18;6(0):36698. 2016-11-18 [PMID: 27857162] (IHC-Fr, Mouse)
• Wnt4 is a novel biomarker for the early detection of kidney tubular injury after ischemia/reperfusion injury Sci Rep, 2016-09-07;6(0):32610. 2016-09-07 [PMID: 27600466] (IHC, Western Blot, Mouse)
• Ishida R, Kami D, Kusaba T, Kirita Y, Kishida T, Mazda O, Adachi T, Gojo S Kidney-specific Sonoporation-mediated Gene Transfer. Mol Ther, 2015-09-30;24(1):125-34. 2015-09-30 [PMID: 26419704] (IHC, Western Blot, Mouse)
• Moller-Tank S, Albritton L, Rennert P, Maury W Characterizing functional domains for TIM-mediated enveloped virus entry. J Virol, 2014-04-02;88(12):6702-13. 2014-04-02 [PMID: 24696470] (Flow Cytometry, Human)
• Long-term rescue of dystrophin expression and improvement in muscle pathology and function in dystrophic mdx mice by peptide-conjugated morpholino. Am. J. Pathol., 2012-06-07;181(2):392-400. 2012-06-07 [PMID: 22683468] (Western Blot, Mouse)
• Humphreys BD, Czerniak S, DiRocco DP Repair of injured proximal tubule does not involve specialized progenitors. Proc. Natl. Acad. Sci. U.S.A., 2011-05-16;108(22):9226-31. 2011-05-16 [PMID: 21576461] (IHC-P, Mouse)
• Nakae S, Iikura M, Suto H, Akiba H, Umetsu DT, DeKruyff RH, Saito H, Galli SJ TIM-1 and TIM-3 enhancement of Th2 cytokine production by mast cells. Blood, 2007-07-09;110(7):2565-8. 2007-07-09 [PMID: 17620455] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: HAVCR1
• Uniprot:
  • NP_001160104()