Galectin-3 Antibody [Unconjugated]

Images

 
Western blot shows lysates of MCF‑7 human breast cancer cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, C6 rat glioma cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane ...read more
Galectin‑3 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c., cross-section through the eye) using Goat Anti-Human Galectin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1197 ...read more
Simple Western lane view shows lysates of HT‑2 mouse T cell line and Balb/3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑3 at approximately 44 kDa (as ...read more
Western blot shows lysates of 4T1 mouse breast cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line and HT‑2 mouse T cell line. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Mouse Galectin‑3 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Galectin-3 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse Galectin‑3
Ala2-Ile264
Accession # NP_034835
Specificity
Detects human, mouse, and rat Galectin-3 in Western blots. Detects mouse Galectin-3 in direct ELISAs. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Galectin-3 is observed and less than 1% cross-reactivity with recombinant mouse (rm) Galectin-4 and rmGalectin-9 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LGALS3
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.05-0.2 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF1197 in the following applications:

Publications
Read Publications using
AF1197 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-3 Antibody [Unconjugated]

  • AGE-R3
  • Carbohydrate-binding protein 35
  • CBP 35,35 kDa lectin
  • CBP35
  • GAL3
  • Gal-3
  • galactin-3
  • Galactose-specific lectin 3
  • Galactoside-binding protein
  • GALBPCBP35
  • Galectin3
  • Galectin-3
  • GALIG
  • IgE-binding protein
  • L29
  • L31
  • L-31
  • Laminin-binding protein
  • Lectin L-29
  • lectin, galactoside-binding, soluble, 3
  • LGALS2
  • LGALS3
  • Mac-2 antigen
  • Mac-2
  • MAC2GAL3

Background

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4).

Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is a chimera galectin that has a tendency to dimerize. Besides the soluble protein, alternatively spliced forms of chicken Galectin-3 containing a transmembrane-spanning domain and a leucine zipper motif have been reported. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells, and fibroblasts. It binds various matrix glycoproteins including laminin, fibronectin, LAMPS, 90K/Mac-2BP, MP20, and CEA. Galectin-3 promotes cell growth and proliferation for many cell types. Galectin-3 acts intracellularly to prevent apoptosis. Depending on the cell types, Galectin-3 exhibits pro- or anti-adhesive properties. Galectin-3 has proinflammatory activities in vitro and in vivo. It induces pro-inflammatory and inhibits Th2 type cytokine production. Galectin-3 chemoattracts monocytes and macrophages. It activates and degranulates basophils and mast cells. Elevated circulating levels of Galectin-3 has been show to correlate with the malignant potential of several types of cancer, suggesting that Galectin-3 is also involved in tumor growth and metastasis. Human and mouse Galectin-3 shares approximately 80% amino acid sequence similarity (1-5).

  1. Rabinovich, A. et al. (2002) Trends in Immunol. 23:313.
  2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
  3. Hughes, R.C. (2001) Biochimie 83:667.
  4. R&D Systems Cytokine Bulletin; Summer 2002.
  5. Gorski, J.P. et al. (2002) J. Biol. Chem. 277:18840.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Galectin-3 Antibody (AF1197)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, IHC-P, Western Blot.


Filter By Application
IHC
(3)
IHC-P
(1)
Western Blot
(3)
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Human
(1)
Mouse
(6)
All Species
Showing Publications 1 - 9 of 9.
Publications using AF1197 Applications Species
T Iwanaga, J Nio-Kobaya, H Takahashi- Bush-like integrin filament networks associated with hyaloid vasculature in murine neonate eyes Biomed. Res., 2019;40(2):79-85. 2019 [PMID: 30982803] (IHC, Mouse) IHC Mouse
I García-Dom, K Veselá, J García-Rev, A Carrillo-J, MA Roca-Cebal, M Santiago, RM de Pablos, JL Venero Peripheral Inflammation Enhances Microglia Response and Nigral Dopaminergic Cell Death in an in vivo MPTP Model of Parkinson's Disease Front Cell Neurosci, 2018;12(0):398. 2018 [PMID: 30459561]
A Alawieh, M Andersen, DL Adkins, S Tomlinson Acute Complement Inhibition Potentiates Neurorehabilitation and Enhances tPA-Mediated Neuroprotection J. Neurosci., 2018;38(29):6527-6545. 2018 [PMID: 29921716]
A Kishimoto, S Kimura, J Nio-Kobaya, H Takahashi-, AM Park, T Iwanaga Histochemical characteristics of regressing vessels in the hyaloid vascular system of neonatal mice: Novel implication for vascular atrophy Exp. Eye Res., 2018;172(0):1-9. 2018 [PMID: 29596849] (IHC, Mouse) IHC Mouse
A Alawieh, EF Langley, S Weber, D Adkins, S Tomlinson Identifying the role of complement in triggering neuroinflammation after traumatic brain injury J. Neurosci., 2018;0(0):. 2018 [PMID: 29437855] (IHC, Mouse) IHC Mouse
Kr�ppel-like factor 3 (KLF3/BKLF) is required for widespread repression of the inflammatory modulator galectin-3 (Lgals3) J Biol Chem, 2016;0(0):. 2016 [PMID: 27226561] (Western Blot, Mouse) Western Blot Mouse
Tsai CM, Guan CH, Hsieh HW, Hsu TL, Tu Z, Wu KJ, Lin CH, Lin KI Galectin-1 and galectin-8 have redundant roles in promoting plasma cell formation. J. Immunol., 2011;187(4):1643-52. 2011 [PMID: 21753146] (Western Blot, Mouse) Western Blot Mouse
Buhimschi CS, Baumbusch MA, Dulay AT, Oliver EA, Lee S, Zhao G, Bhandari V, Ehrenkranz RA, Weiner CP, Madri JA, Buhimschi IA Characterization of RAGE, HMGB1, and S100beta in inflammation-induced preterm birth and fetal tissue injury. Am. J. Pathol., 2009;175(3):958-75. 2009 [PMID: 19679874] (IHC-P, Human) IHC-P Human
Papaspyridonos M, McNeill E, de Bono JP, Smith A, Burnand KG, Channon KM, Greaves DR Galectin-3 is an amplifier of inflammation in atherosclerotic plaque progression through macrophage activation and monocyte chemoattraction. Arterioscler. Thromb. Vasc. Biol., 2007;28(3):433-40. 2007 [PMID: 18096829] (Western Blot, Mouse) Western Blot Mouse

Reviews for Galectin-3 Antibody (AF1197) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.

Reviews using AF1197:
Filter by Applications
ICC
(1)
IHC-P
(1)
All Applications
Filter by Species
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ICC Rat 01/05/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 22981630
SpeciesRat

Blocking

Blocking DetailsSee PMID 22981630

Details

Detection NotesSee PMID 22981630

Comments

Comments"Galectin-3 antibody dilution: 1:400 in 5% Rabbit serum.Incubated with anti-Galectin ab overnight at 4 deg C. The staining was visualized using Vectastain Elite kit and DAB as peroxidase substrate."PMID 22981630
 
reviewed by:
Anonymous
IHC-P Mouse 01/05/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 23175698
SpeciesMouse

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Biotin BAF1197

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
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Anonymous
01/05/2015
Application: ICC
Species: Rat

 
Anonymous
01/05/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol LGALS3
Uniprot