E. coli-derived recombinant human Galectin-4 (R&D Systems, Catalog # 1227-GA) Ala2-Ile323 Accession # P56470
Detects human Galectin-4 in Western blots. In this format, approximately 50% cross-reactivity with recombinant mouse Galectin-4 is observed and less than 2% cross-reactivity with recombinant human (rh) Galectin-1, rhGalectin-2, rhGalectin-3, rhGalectin-7 and rhGalectin-8 is observed.
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Galectin-4 Antibody [Biotin]
L-36 lactose-binding protein
Lactose-binding lectin 4
lectin, galactoside-binding, soluble, 4
Galectins are a family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins share structural similarities in their carbohydrate recognition domains (CRD), forming three groups often termed prototype (one CRD), tandem-repeat (two CRDs) and chimeric (one CRD, unique N-terminus) (1, 2). All lack classical signal peptides, but are present and active both within and outside of the cell. Galectins are involved in cell adhesion, migration, survival and apoptosis, and are often up- or down-regulated in cancer (1‑3). Galectin-4 is a 36 kDa tandem-repeat galectin found throughout the gastrointestinal tract, but also present in well-differentiated breast and liver carcinomas (3, 4). Each CRD binds a different set of carbohydrate groups, including those found on red cell blood group antigens (3, 5). CRD1 also binds cholesterol 3-sulfate and other sulfatides, which are concentrated within lipid raft membrane microdomains (6, 7). Endocytosed Galectin-4 is thought to play a role in forming the rafts, delivering them to the intestinal apical membrane, and stabilizing highly detergent-resistant "superrafts" (7, 9). Human Galectin-4 shares 76%, 77%, 78% and 80% aa identity with mouse, rat, bovine and porcine Galectin-4, respectively, with the highest identity occurring within the CRDs. A potential splice variant begins at aa 132 and lacks most of the first CRD (10). Galectin-4 expression is concentrated within microvilli in the gastrointestinal epithelium, where it can interact with CD3 and bind activated T cells in the lamina propria during intestinal inflammation (11, 12). Either pro- or anti-inflammatory activity has been shown, depending on the mouse model used. Galectin-4 can also bind lung, spleen and kidney macrophages, although its expression is normally low in these tissues (5).
Yang, R-Y. et al. (2008) Expert Rev. Mol. Med. 10:e17.
Elola, M. T. et al. (2007) Cell. Mol. Life Sci. 64:1679.
Huflejt, M. E. & H. Leffler (2004) Glycoconj. J. 20:247.
Recreche, H. et al. (1997) Eur. J. Biochem. 248:225.
Markova, V. et al. (2006) Int. J. Mol. Med. 18:65.
Ideo, H. et al. (2007) J. Biol. Chem. 282:21081.
Delacour, D. et al. (2005) J. Cell Biol. 169:491.
Braccia, A. et al. (2003) J. Biol. Chem. 278:15679.
Stechly, L. et al. (2009) Traffic Jan 24 e-pub ahead of print.
Entrez Accession # EAW56820.
Hokama, A. et al. (2004) Immunity 20:681.
Paclik, D. et al. (2008) PloS ONE 3:e2629.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.