Galectin-4 Antibody [Unconjugated]


Western blot shows lysates of human, mouse, and rat colon tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Galectin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # more
Simple Western lane view shows lysates of human and mouse colon tissue, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑4 at approximately 44 kDa (as indicated) using 5 µg/mL of Goat more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC

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Galectin-4 Antibody [Unconjugated] Summary

E. coli-derived recombinant human Galectin-4
Accession # P56470
Detects human Galectin-4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) Galectin-8 and rhGalectin-9 is observed.
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  • Western Blot 0.5 ug/mL
  • Simple Western 5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
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AF1227 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-4 Antibody [Unconjugated]

  • Antigen NY-CO-27
  • GAL4
  • gal-4
  • galectin 4
  • Galectin4
  • Galectin-4
  • L-36 lactose-binding protein
  • L36LBP
  • Lactose-binding lectin 4
  • lectin, galactoside-binding, soluble, 4
  • LGALS4


Galectins are a family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins share structural similarities in their carbohydrate recognition domains (CRD), forming three groups often termed prototype (one CRD), tandem-repeat (two CRDs) and chimeric (one CRD, unique N-terminus) (1, 2). All lack classical signal peptides, but are present and active both within and outside of the cell. Galectins are involved in cell adhesion, migration, survival and apoptosis, and are often up- or down-regulated in cancer (1‑3). Galectin-4 is a 36 kDa tandem-repeat galectin found throughout the gastrointestinal tract, but also present in well-differentiated breast and liver carcinomas (3, 4). Each CRD binds a different set of carbohydrate groups, including those found on red cell blood group antigens (3, 5). CRD1 also binds cholesterol 3-sulfate and other sulfatides, which are concentrated within lipid raft membrane microdomains (6, 7). Endocytosed Galectin-4 is thought to play a role in forming the rafts, delivering them to the intestinal apical membrane, and stabilizing highly detergent-resistant "superrafts" (7, 9). Human Galectin-4 shares 76%, 77%, 78% and 80% aa identity with mouse, rat, bovine and porcine Galectin-4, respectively, with the highest identity occurring within the CRDs. A potential splice variant begins at aa 132 and lacks most of the first CRD (10). Galectin-4 expression is concentrated within microvilli in the gastrointestinal epithelium, where it can interact with CD3 and bind activated T cells in the lamina propria during intestinal inflammation (11, 12). Either pro- or anti-inflammatory activity has been shown, depending on the mouse model used. Galectin-4 can also bind lung, spleen and kidney macrophages, although its expression is normally low in these tissues (5).


  1. Yang, R-Y. et al. (2008) Expert Rev. Mol. Med. 10:e17.
  2. Elola, M. T. et al. (2007) Cell. Mol. Life Sci. 64:1679.
  3. Huflejt, M. E. & H. Leffler (2004) Glycoconj. J. 20:247.
  4. Recreche, H. et al. (1997) Eur. J. Biochem. 248:225.
  5. Markova, V. et al. (2006) Int. J. Mol. Med. 18:65.
  6. Ideo, H. et al. (2007) J. Biol. Chem. 282:21081.
  7. Delacour, D. et al. (2005) J. Cell Biol. 169:491.
  8. Braccia, A. et al. (2003) J. Biol. Chem. 278:15679.
  9. Stechly, L. et al. (2009) Traffic Jan 24 e-pub ahead of print.
  10. Entrez Accession # EAW56820.
  11. Hokama, A. et al. (2004) Immunity 20:681.
  12. Paclik, D. et al. (2008) PloS ONE 3:e2629.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Galectin-4 Antibody (AF1227)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA Development.

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Secondary Antibodies


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Biotin BAF1227

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Gene Symbol LGALS4
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