G9a/EHMT2 Antibody

Images

 
Western Blot: G9a/EHMT2 Antibody [NBP2-55881] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: G9a/EHMT2 Antibody [NBP2-55881] - Staining of human cell line MCF7 shows localization to nucleoplasm & nuclear speckles. Antibody staining is shown in green.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

G9a/EHMT2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: PRSEETLPKATPDSLEPAGPSSPASVTVTVGDEGADTPVGATPLIGDESENLEGDGDLRGGRILLGHATKSFPSS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EHMT2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
G9a/EHMT2 Recombinant Protein Antigen (NBP2-55881PEP)

Reactivity Notes

Mouse 85%, Rat 87%

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for G9a/EHMT2 Antibody

  • ankyrin repeat-containing protein
  • BAT8
  • C6orf30
  • chromosome 6 open reading frame 30
  • DKFZp686H08213
  • EC 2.1.1.-
  • EC 2.1.1.43
  • EHMT2
  • euchromatic histone-lysine N-methyltransferase 2HLA-B-associated transcript 8
  • FLJ35547
  • G9A histone methyltransferase
  • G9a
  • G9AEm:AF134726.3
  • H3-K9-HMTase 3
  • Histone H3-K9 methyltransferase 3
  • histone-lysine N-methyltransferase, H3 lysine-9 specific 3
  • HLA-B associated transcript 8
  • KMT1CNG36/G9a
  • Lysine N-methyltransferase 1C
  • NG36
  • Protein G9a

Background

G9A antibody contains ankyrin repeats, suggesting involvement in intracellular protein-protein interaction. G9A is a histone methyltransferase that preferentially methylates Lys-9 of histone H3 and Lys-27 of histone H3 (in vitro). H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. G9A is probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol EHMT2