FXR/NR1H4 Antibody

Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody [NB100-55416] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Flow Cytometry: FXR/NR1H4 Antibody [NB100-55416] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Bv
• Applications: ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

FXR/NR1H4 Antibody Summary

• Immunogen: Peptide with sequence KSCREKTELTPDQQ, from the internal region of the protein sequence according to NP_005114.1; NP_001193906.1; NP_001193922.1; NP_001193921.1; NP_001193907.1.
• Epitope: KSCREKTELTPDQQ
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NR1H4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary testing showed a band at approx 55kDa in Human Kidney, Colon, Duodenum and Ileum lysate after 0.5ug/ml antibody staining (calculated MW of 54.4kDa according to NP_005114.1). Primary incubation 1 hour at room temperature.
• Control:

  FXR/NR1H4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FXR/NR1H4 Antibody

• BAR
• bile acid receptor
• farnesoid X receptor
• Farnesoid X-activated receptor
• Farnesol receptor HRR-1
• FXR
• FXRBAR
• HRR-1
• HRR1MGC163445
• NR1H4
• Nuclear receptor subfamily 1 group H member 4
• nuclear receptor subfamily 1, group H, member 4
• Retinoid X receptor-interacting protein 14
• RIP14
• RIP14RXR-interacting protein 14

Background

Bile Acid Receptor NR1H4 is a receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. It represses the transcription of the cholesterol 7 alpha hydroxylase gene and activates the intestinal bile acid binding protein. The NR1H4 gene encodes a protein that has a central role in lipid homeostasis and also regulates carbohydrate metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FXR/NR1H4 Antibody (NB100-55416)(1)

Publication using NB100-55416

• Huang W, Ma K, Zhang J et al. Nuclear receptor-dependent bile acid signaling is required for normal liver regeneration. Science 2006-04-14 [PMID: 16614213]

Bioinformatics

• Gene Symbol: NR1H4
• Entrez:
  • 9971(Human)
• Uniprot:
  • NP_005114.1(Human)