FoxP3

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FoxP3 [NBP2-29545] - FOXP3 [NBP2-29545] - Schematic representation

Product Details

Summary
Reactivity HuSpecies Glossary
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Order Details

FoxP3 Summary

Immunogen
Foxp3, human (full length cDNA) in PCLXSN Retroviral expression vector
Gene
FOXP3

Applications/Dilutions

Application Notes
The RetroMax system is designed for maximal virus titer in 293 cells. It takes advantages of two properties of 293 cells, i) high level of transfectibility, ii) strong E1A-mediated stimulation of CMV promoter controlled transcription. 293 cells are nonmurine in origin, hence avoiding the problem of selective packaging and transfer of VL30 genomes (present in all murine packaging cells). Vector supernatants are free of helper virus and are of sufficiently high titer within 2 days of transient transfection in 293 cells to permit infection of more than 50% of dividing target cells in culture.
Amplify the plasmid DNA by transforming on to a suitable competent bacteria, e.g. DH5alpha or XL-1 Blue. Plate on LB agar plate containing 50ug/ml ampicillin. Start a culture from a single colony for plasmid DNA preparation.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
20 ul 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Alternate Names for FoxP3

  • AIID
  • AIIDMGC141961
  • DIETER
  • forkhead box P3
  • Forkhead Box Protein P3
  • FoxP3
  • FOXP3delta7
  • immune dysregulation, polyendocrinopathy, enteropathy, X-linked
  • Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
  • IPEX
  • JM2
  • MGC141961
  • MGC141963
  • PIDX
  • PIDXMGC141963
  • SCURFIN
  • XPID
  • XPIDpolyendocrinopathy, enteropathy, X-linked

Background

20 ul 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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FAQs for FoxP3 (NBP2-29545). (Showing 1 - 2 of 2 FAQ).

  1. Does FoxP3 antibodies comes in lyophilized form?
    • FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
  2. What the theoretical molecular weight for FoxP3 antibodies?
    • The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.

Additional FoxP3 Products

Bioinformatics Tool for FoxP3 (NBP2-29545)

Discover related pathways, diseases and genes to FoxP3 (NBP2-29545). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for FoxP3 (NBP2-29545)

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Pathways for FoxP3 (NBP2-29545)

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PTMs for FoxP3 (NBP2-29545)

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Research Areas for FoxP3 (NBP2-29545)

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Blogs on FoxP3.

Meeting Report: 2nd International Antibody Validation Meeting
Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde...  Read full blog post.

FOXP3
Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function.  Distinctively,...  Read full blog post.

FOXP3 is a Master Regulator of T Regulatory (Treg) Cells
FOXP3, a member of forkhead/winged-helix family of transcription factors acts as a "master" regulator for the development and suppressive function of regulatory T cells (Tregs). Its constitutive expression is necessary for the suppressive function of...  Read full blog post.

FOXP3: Master Regulatory Transcriptional Factor
FOXP3, a forkhead family transcription factor specially expressed in regulatory T (Treg) cells, controls the expression of many key immune-regulatory genes. Treg cells are a population of T lymphocytes that have critical roles in the immune system hom...  Read full blog post.

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Bioinformatics

Gene Symbol FOXP3