FIH-1/HIF-1AN Antibody (162c) [DyLight 650]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
162c
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 650

FIH-1/HIF-1AN Antibody (162c) [DyLight 650] Summary

Immunogen
Full length human Factor Inhibiting HIF-1 protein [Swiss-Prot# Q9NWT6]
Localization
Nuclear
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HIF1AN
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This Factor Inhibiting HIF-1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot analysis where a band can be seen at 40 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for FIH-1/HIF-1AN Antibody (162c) [DyLight 650]

  • DKFZp762F1811
  • EC 1.14.11.16
  • factor inhibiting HIF1
  • Factor inhibiting HIF-1
  • FIH1
  • FIH-1
  • FIH1FIH-1
  • FLJ20615
  • FLJ22027
  • HIF1AN
  • HIF-1AN
  • hypoxia inducible factor 1, alpha subunit inhibitor
  • hypoxia-inducible factor 1-alpha inhibitor
  • Hypoxia-inducible factor asparagine hydroxylase
  • Peptide-aspartate beta-dioxygenase

Background

Factor Inhibiting HIF1 (FIH) protein facilitates repression of HIF-1 transcriptional activity by binding to VHL, which acts as a transcriptional corepressor. VHL inhibits HIF-1 alpha transactivation function by recruiting histone deacetylases. Involvement of VHL in association with FIH provides a unifying mechanism for the modulation of HIF-1 alpha protein stabilization and transcriptional activation in response to changes in cellular oxygen concentration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FIH-1/HIF-1AN Antibody (NBP1-30333C) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP1-30333B
DyLight 350 Labeled NBP1-30333UV
DyLight 405 Labeled NBP1-30333V
DyLight 488 Labeled NBP1-30333G
DyLight 550 Labeled NBP1-30333R
DyLight 680 Labeled NBP1-30333FR
DyLight 755 Labeled NBP1-30333IR
FITC Labeled NBP1-30333F
HRP Labeled NBP1-30333H

Additional FIH-1/HIF-1AN Products

Bioinformatics Tool for FIH-1/HIF-1AN Antibody (NBP1-30333C)

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Diseases for FIH-1/HIF-1AN Antibody (NBP1-30333C)

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PTMs for FIH-1/HIF-1AN Antibody (NBP1-30333C)

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Research Areas for FIH-1/HIF-1AN Antibody (NBP1-30333C)

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Bioinformatics

Gene Symbol HIF1AN
Entrez