FIH-1/HIF-1AN Antibody (162c)

Images

 
Western Blot: Factor Inhibiting HIF-1 Antibody (162c) [NBP1-30333] - Analysis in A431 cell lysates using NBP1-30333.
Immunocytochemistry/ Immunofluorescence: Factor Inhibiting HIF-1 Antibody (162c) [NBP1-30333] - FIH (162C) antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi ...read more
Immunohistochemistry: Factor Inhibiting HIF-1 Antibody (162c) [NBP1-30333] - Analysis of FIH in human renal cancer using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
162c
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

FIH-1/HIF-1AN Antibody (162c) Summary

Immunogen
Full length human Factor Inhibiting HIF-1 protein [Swiss-Prot# Q9NWT6]
Localization
Nuclear
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HIF1AN
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:25
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Application Notes
This Factor Inhibiting HIF-1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot analysis where a band can be seen at 40 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Jurkat Lysate (NB800-PC2)
A-431 Lysate (NB800-PC12)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-30333 in the following applications:

Publications
Read Publications using
NBP1-30333 in the following applications:

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for FIH-1/HIF-1AN Antibody (162c)

  • DKFZp762F1811
  • EC 1.14.11.16
  • factor inhibiting HIF1
  • Factor inhibiting HIF-1
  • FIH1
  • FIH-1
  • FIH1FIH-1
  • FLJ20615
  • FLJ22027
  • HIF1AN
  • HIF-1AN
  • hypoxia inducible factor 1, alpha subunit inhibitor
  • hypoxia-inducible factor 1-alpha inhibitor
  • Hypoxia-inducible factor asparagine hydroxylase
  • Peptide-aspartate beta-dioxygenase

Background

Factor inhibiting HIF1 (FIH1) is an asparaginyl hydroxylase enzyme that regulates HIF's transcriptional activity by hydroxylating HIF-1 alpha at 'Asp-803' in the CAD (C-terminal transactivation domain). Hydroxylation via PHDs followed by proteosome degradation is another mechanism that regulates HIF1, however, FIH1 is able to exercise the control even under severe hypoxic conditions, when PHD enzymes fail to do so. Localized mainly in cytoplasm, FIH1 functions as oxygen sensor and FIH1 interaction with NOTCH1 results in its nuclear localization whereas FIH1-ABPA3 interaction leads to its perinuclear localization. During normoxic conditions, FIH1 hydroxylates a conserved asparaginyl residue within CTAD of HIF-1 alpha/HIF-2 alpha, leading to steric clash that prevents the recruitment of the co-activators p300 and CBP. On the other hand, under hypoxic conditions, FIH1 is inactive, which results in activation of HIF-alpha signaling. FIH1 is involved in transcriptional repression through interaction with HIF1A, VHL and HDAC and hydroxylates specific Asn residues within ARD (ankyrin repeat domains) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A as well as other ARD-containing proteins. FIH1 also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. FIH1 has the ability to negatively regulate NOTCH1 activity thus accelerating myogenic differentiation, and positively regulates ASB4 activity which promotes vascular differentiation. FIH1-Mint3 binding affects FIH1's ability to modify HIF-1 alpha in an oxygen-independent manner and FIH1-Bax interaction plays inhibitory role in apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-105
Species: Hu, Mu, Rt, Po, Bv, Ft, Mk, Pm, Rb, Sh, Xp, Ye
Applications: WB, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, TCS, KO, LA
NB120-19347
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IF
NB100-137
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP
NB100-616
Species: Hu, Mu, Rt, Md, Pm
Applications: WB, ChIP, EM, ICC/IF, IP, In vitro, Flow-IC
NBP1-89843
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-485
Species: Hu
Applications: WB, IP
NB100-139
Species: Hu, Mu, Rt
Applications: WB, EM, ICC/IF, IHC, IHC-P, IP
NB100-417
Species: Hu, Mu, Rt, Ca, Pl
Applications: WB, Simple Western, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-381
Species: Hu
Applications: WB, ChIP, IP, PLA
NB500-487
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P, IP
H00005956-R02
Species: Hu
NBP1-76729
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB100-81898
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
AF6394
Species: Hu
Applications: WB, IP
AF5689
Species: Hu
Applications: WB
MAB9181
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready
NB100-122
Species: Hu, Mu, Rt, Fi, Ha, Pm, Sh
Applications: WB, Simple Western, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO
NBP1-30333
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for FIH-1/HIF-1AN Antibody (NBP1-30333)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(2)
WB
(5)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-30333 Applications Species
Mysore VS, Szablowski J, Dervan PB, Frost PJ. A DNA-binding Molecule Targeting the Adaptive Hypoxic Response in Multiple Myeloma Has Potent Antitumor Activity. Mol Cancer Res. [PMID: 26801054] (WB, Human) WB Human
Wollenick K, Hu J, Kristiansen G et al. Synthetic transactivation screening reveals ETV4 as broad coactivator of hypoxia-inducible factor signaling. Nucleic Acids Research 2011 Nov 10 [PMID: 22075993] (WB, Human) WB Human
Khan MN, Bhattacharyya T, Andrikopoulos P et al. Factor inhibiting HIF (FIH-1) promotes renal cancer cell survival by protecting cells from HIF-1alpha-mediated apoptosis Br J Cancer 2011 Mar 29 [PMID: 21386837] (WB, Human) WB Human
Yan M, Rayoo M, Takano EA et al. BRCA1 tumours correlate with a HIF-1alpha phenotype and have a poor prognosis through modulation of hydroxylase enzyme profile expression. Br J Cancer;101(7):1168-74. 2009 Oct 6. [PMID: 19724277] (IHC-P, Human) IHC-P Human
Cockman ME, Lancaster DE, Stolze IP, Hewitson KS, McDonough MA, Coleman ML, Coles CH, Yu X, Hay RT, Ley SC, Pugh CW, Oldham NJ, Masson N, Schofield CJ, Ratcliffe PJ. Posttranslational hydroxylation of ankyrin repeats in IkappaB proteins by the hypoxia-inducible factor (HIF) asparaginyl hydroxylase, factor inhibiting HIF (FIH). Proc Natl Acad Sci U S A;103(40):14767-72. 2006 Oct 3. [PMID: 17003112] (WB, Human) WB Human
Soilleux EJ et al. Use of novel monoclonal antibodies to determine the expression and distribution of the hypoxia regulatory factors PHD-1, PHD-2, PHD-3 and FIH in normal and neoplastic human tissues. Histopathology (47) 602-610. -2005 [PMID: 16324198] (IHC-P, Human) IHC-P Human
Stolze IP et al. Genetic analysis of the role of the asparaginyl hydroxylase factor inhibiting hypoxia-inducible factor (HIF) in regulating HIF transcriptional target genes. J Bio Chem 42719-42725. 2004 [PMID: 15302861] (WB, ICC/IF, Human) WB, ICC/IF Human

Review for FIH-1/HIF-1AN Antibody (NBP1-30333) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-30333:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot FIH-1/HIF-1AN NBP1-30333
Enlarge
reviewed by:
Anonymous
WB Human 10/23/2015
View

Summary

ApplicationWestern Blot
Sample TestedRPMI 8226 Cells, whole cell lysate
SpeciesHuman
LotA1

Blocking

Blocking Details5% milk in PBST, 30 minutes at Room Temperature

Primary Anitbody

Dilution Ratio1:1000, Overnight, 4 C, PBST with 5%milk

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG-HRP
Secondary Manufacturer Cat#SC-2005
Secondary Concentration1:2000

Details

Detection NotesChemiluminescence, G:Box Chemi XLT4, 10 sec, no positive and negative controls

Comments

CommentsPrimary antibody can be used at a dilution above 1:1000, since the signal was very bright just after 10 seconds.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FIH-1/HIF-1AN Antibody (NBP1-30333) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-30333AF405
Alexa Fluor 488 Labeled NBP1-30333AF488
Alexa Fluor 647 Labeled NBP1-30333AF647
Alexa Fluor 700 Labeled NBP1-30333AF700
Biotin Labeled NBP1-30333B
DyLight 350 Labeled NBP1-30333UV
DyLight 405 Labeled NBP1-30333V
DyLight 488 Labeled NBP1-30333G
DyLight 550 Labeled NBP1-30333R
DyLight 650 Labeled NBP1-30333C
DyLight 680 Labeled NBP1-30333FR
DyLight 755 Labeled NBP1-30333IR
FITC Labeled NBP1-30333F
HRP Labeled NBP1-30333H

Additional FIH-1/HIF-1AN Products

Bioinformatics Tool for FIH-1/HIF-1AN Antibody (NBP1-30333)

Discover related pathways, diseases and genes to FIH-1/HIF-1AN Antibody (NBP1-30333). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FIH-1/HIF-1AN Antibody (NBP1-30333)

Discover more about diseases related to FIH-1/HIF-1AN Antibody (NBP1-30333).
 

Pathways for FIH-1/HIF-1AN Antibody (NBP1-30333)

View related products by pathway.

PTMs for FIH-1/HIF-1AN Antibody (NBP1-30333)

Learn more about PTMs related to FIH-1/HIF-1AN Antibody (NBP1-30333).
 

Research Areas for FIH-1/HIF-1AN Antibody (NBP1-30333)

Find related products by research area.

Blogs on FIH-1/HIF-1AN

There are no specific blogs for FIH-1/HIF-1AN, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
10/23/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol HIF1AN
Entrez
Uniprot