FIH-1/HIF-1AN Antibody (162c)


Western Blot: Factor Inhibiting HIF-1 Antibody (162c) [NBP1-30333] - Analysis in A431 cell lysates using NBP1-30333.
Immunocytochemistry/ Immunofluorescence: Factor Inhibiting HIF-1 Antibody (162c) [NBP1-30333] - FIH (162C) antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi more
Immunohistochemistry: Factor Inhibiting HIF-1 Antibody (162c) [NBP1-30333] - Analysis of FIH in human renal cancer using DAB with hematoxylin counterstain.

Product Details

Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
1 mg/ml

FIH-1/HIF-1AN Antibody (162c) Summary

Full length human Factor Inhibiting HIF-1 protein [Swiss-Prot# Q9NWT6]
Protein G purified
Innovator's Reward
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
1 mg/ml
Protein G purified


  • Western Blot 5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:25
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Application Notes
This Factor Inhibiting HIF-1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot analysis where a band can be seen at 40 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
40 kDa
Reviewed Applications
Read 1 Review rated 5
NBP1-30333 in the following applications:

Read Publications using
NBP1-30333 in the following applications:

Reactivity Notes


Alternate Names for FIH-1/HIF-1AN Antibody (162c)

  • DKFZp762F1811
  • EC
  • factor inhibiting HIF1
  • Factor inhibiting HIF-1
  • FIH1
  • FIH1FIH-1
  • FLJ20615
  • FLJ22027
  • HIF1AN
  • HIF-1AN
  • hypoxia inducible factor 1, alpha subunit inhibitor
  • hypoxia-inducible factor 1-alpha inhibitor
  • Hypoxia-inducible factor asparagine hydroxylase
  • Peptide-aspartate beta-dioxygenase


Factor inhibiting HIF1 (FIH1) is an asparaginyl hydroxylase enzyme that regulates HIF's transcriptional activity by hydroxylating HIF-1 alpha at 'Asp-803' in the CAD (C-terminal transactivation domain). Hydroxylation via PHDs followed by proteosome degradation is another mechanism that regulates HIF1, however, FIH1 is able to exercise the control even under severe hypoxic conditions, when PHD enzymes fail to do so. Localized mainly in cytoplasm, FIH1 functions as oxygen sensor and FIH1 interaction with NOTCH1 results in its nuclear localization whereas FIH1-ABPA3 interaction leads to its perinuclear localization. During normoxic conditions, FIH1 hydroxylates a conserved asparaginyl residue within CTAD of HIF-1 alpha/HIF-2 alpha, leading to steric clash that prevents the recruitment of the co-activators p300 and CBP. On the other hand, under hypoxic conditions, FIH1 is inactive, which results in activation of HIF-alpha signaling. FIH1 is involved in transcriptional repression through interaction with HIF1A, VHL and HDAC and hydroxylates specific Asn residues within ARD (ankyrin repeat domains) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A as well as other ARD-containing proteins. FIH1 also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. FIH1 has the ability to negatively regulate NOTCH1 activity thus accelerating myogenic differentiation, and positively regulates ASB4 activity which promotes vascular differentiation. FIH1-Mint3 binding affects FIH1's ability to modify HIF-1 alpha in an oxygen-independent manner and FIH1-Bax interaction plays inhibitory role in apoptosis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FIH-1/HIF-1AN Antibody (NBP1-30333)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.

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Showing Publications 1 - 7 of 7.
Publications using NBP1-30333 Applications Species
Mysore VS, Szablowski J, Dervan PB, Frost PJ. A DNA-binding Molecule Targeting the Adaptive Hypoxic Response in Multiple Myeloma Has Potent Antitumor Activity. Mol Cancer Res. [PMID:26801054] (WB, Human) WB Human
Wollenick K, Hu J, Kristiansen G et al. Synthetic transactivation screening reveals ETV4 as broad coactivator of hypoxia-inducible factor signaling. Nucleic Acids Research 2011 Nov 10 [PMID:22075993] (WB, Human) WB Human
Khan MN, Bhattacharyya T, Andrikopoulos P et al. Factor inhibiting HIF (FIH-1) promotes renal cancer cell survival by protecting cells from HIF-1alpha-mediated apoptosis Br J Cancer 2011 Mar 29 [PMID:21386837] (WB, Human) WB Human
Yan M, Rayoo M, Takano EA et al. BRCA1 tumours correlate with a HIF-1alpha phenotype and have a poor prognosis through modulation of hydroxylase enzyme profile expression. Br J Cancer;101(7):1168-74. 2009 Oct 6. [PMID:19724277] (IHC-P, Human) IHC-P Human
Cockman ME, Lancaster DE, Stolze IP, Hewitson KS, McDonough MA, Coleman ML, Coles CH, Yu X, Hay RT, Ley SC, Pugh CW, Oldham NJ, Masson N, Schofield CJ, Ratcliffe PJ. Posttranslational hydroxylation of ankyrin repeats in IkappaB proteins by the hypoxia-inducible factor (HIF) asparaginyl hydroxylase, factor inhibiting HIF (FIH). Proc Natl Acad Sci U S A;103(40):14767-72. 2006 Oct 3. [PMID:17003112] (WB, Human) WB Human
Soilleux EJ et al. Use of novel monoclonal antibodies to determine the expression and distribution of the hypoxia regulatory factors PHD-1, PHD-2, PHD-3 and FIH in normal and neoplastic human tissues. Histopathology (47) 602-610. -2005 [PMID:16324198] (IHC-P, Human) IHC-P Human
Stolze IP et al. Genetic analysis of the role of the asparaginyl hydroxylase factor inhibiting hypoxia-inducible factor (HIF) in regulating HIF transcriptional target genes. J Bio Chem 42719-42725. 2004 [PMID:15302861] (WB, ICC/IF, Human) WB, ICC/IF Human

Reviews for FIH-1/HIF-1AN Antibody (NBP1-30333) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-30333:
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Images Ratings Applications Species Date Details
Western Blot FIH-1/HIF-1AN NBP1-30333
reviewed by:
WB Human 10/23/2015


ApplicationWestern Blot
Sample TestedRPMI 8226 Cells, whole cell lysate


Blocking Details5% milk in PBST, 30 minutes at Room Temperature

Primary Anitbody

Dilution Ratio1:1000, Overnight, 4 C, PBST with 5%milk

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG-HRP
Secondary Manufacturer Cat#SC-2005
Secondary Concentration1:2000


Detection NotesChemiluminescence, G:Box Chemi XLT4, 10 sec, no positive and negative controls


CommentsPrimary antibody can be used at a dilution above 1:1000, since the signal was very bright just after 10 seconds.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FIH-1/HIF-1AN Antibody (NBP1-30333) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NBP1-30333AF405
Alexa Fluor (R) 488 Labeled NBP1-30333AF488
Alexa Fluor (R) 647 Labeled NBP1-30333AF647
Alexa Fluor (R) 700 Labeled NBP1-30333AF700
Allophycocyanin Labeled NBP1-30333APC
Biotin Labeled NBP1-30333B
DyLight 350 Labeled NBP1-30333UV
DyLight 405 Labeled NBP1-30333V
DyLight 405LS Labeled NBP1-30333V3
DyLight 488 Labeled NBP1-30333G
DyLight 550 Labeled NBP1-30333R
DyLight 650 Labeled NBP1-30333C
DyLight 680 Labeled NBP1-30333FR
DyLight 755 Labeled NBP1-30333IR
FITC Labeled NBP1-30333F
HRP Labeled NBP1-30333H
PE Labeled NBP1-30333PE
PerCP Labeled NBP1-30333PCP

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Contact Information

Product PDFs


Gene Symbol HIF1AN

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