FGF basic/FGF2/bFGF Antibody - Azide and BSA Free

• Reactivity: Hu, Rt, Bv, Eq
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

FGF basic/FGF2/bFGF Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit FGF basic/FGF2/bFGF Antibody - Azide and BSA Free (NB600-1536) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-FGF basic/FGF2/bFGF Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide conjugated to KLH, corresponding to amino acids 1-24 of Cow FGF2.
• Specificity: Does not cross react with bovine FGF Acidic or bovine serum albumin.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FGF2
• Purity: Sterile filtered

Applications/Dilutions

• Dilutions:
  • ELISA indirect
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:1000
• Application Notes: Bioactivity: Anti-FGF basic (1-24) was tested in cell culture using fetal bovine heart endothelial cells (ATCC CRL 1395) plated at low cell density. Various dilutions of Anti-FGF basic(1-24) were pre incubated at 37 C with FGF basic or FGF acidic for 2 hours. Anti-FGF basic (1-24) caused a dose-dependent decrease in the mitogenic activity of FGF basic such that a final dilution of 1:50 neutralized the bioactivity of 1 unit of FGF basic.

Reactivity Notes

Equine reactivity reported in scientific literature (PMID: 24035195).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.2 um filtered solution in 10mM PBS (pH 7.2)
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Sterile filtered
• Reconstitution Instructions: For the 0.1ml size we recommend reconstitution with 0.1ml deionized water. Rotate vial gently until powder dissolves. The 0.025ml size is provided in liquid format.

Alternate Names for FGF basic/FGF2/bFGF Antibody - Azide and BSA Free

• basic fibroblast growth factor bFGF
• Basic fibroblast growth factor
• bFGF
• FGF basic
• FGF2
• FGF-2
• FGFBprostatropin
• fibroblast growth factor 2 (basic)
• HBGF-2
• heparin-binding growth factor 2
• Prostatropin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGF basic/FGF2/bFGF Antibody (NB600-1536)(3)

Publications using NB600-1536

• Hu H, Li W, Wei Y et al. Garcinoic Acid Improves Cardiac Function and Enhances Angiogenesis Following Myocardial Infarction Research Square 2021-02-12
• Katta K, Boersema M, Adepu S et al. Renal Heparan Sulfate Proteoglycans Modulate Fibroblast Growth Factor 2 Signaling in Experimental Chronic Transplant Dysfunction. Am J Pathol. 2013-09-11 [PMID: 24035513] (ELISA, Rat)
• de Ruijter-Villani M, van Boxtel PR, Stout TA. Fibroblast growth factor-2 expression in the preimplantation equine conceptus and endometrium of pregnant and cyclic mares. Theriogenology. 2013-09-11 [PMID: 24035195] (IHC-P, Horse)

FAQs for FGF basic/FGF2/bFGF Antibody (NB600-1536). (Showing 1 - 2 of 2 FAQs).

1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
   • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we
2. What is the catalog of the blocking peptide or protein for this antibody? Or How can I find the blocking peptide or protein for this antibody?
   • Unfortunately we do not have a blocking peptide for this antibody in our catalogue. I am very sorry that we do not have what you are looking for. If you are experiencing non specific bands I would recommend blocking for longer with BSA.

Bioinformatics

• Gene Symbol: FGF2
• Entrez:
  • 281161(Bovine)
  • 2247(Human)
  • 54250(Rat)
• Uniprot:
  • P03969()